Identification of sperm morphometric subpopulations in cooled‐stored canine sperm and its relation with sperm DNA integrity          下载免费PDF全文

M Urbano  I Ortiz  J Dorado  M Hidalgo 《Reproduction in domestic animals》2017,52(3):468-476

The aims of this study were to (i) identify different morphometric subpopulations in cooled‐stored canine sperm and their patterns of distribution during cool‐storage for up to 240 hr and (ii) determine whether or not morphometric sperm subpopulations (sP) are related to sperm DNA integrity. For that purpose, morphometric parameters were analysed by computer‐assisted sperm analysis (CASA) and sperm DNA fragmentation (sDFi) using the sperm Halomax test. Four morphometric sperm heads subpopulations were identified: sP1 (large and rounded), sP2 (large and elongated), sP3 (small and rounded) and sP4 (small and elongated). sP1 was the most predominant subpopulation for up to 72 hr and thereafter sP3 increased progressively. sDFi increased after 48 hr of cool‐storage. Although sP3 showed a positive correlation with sDFi, and both increased over time, it could not be ensured that only the sperm with fragmented DNA are accumulated in sP3. In conclusion, sP3 and DNA fragmentation increased progressively during cool‐storage, becoming possible indicators of sperm damage. However, it cannot be concluded that sP3 only contains sperm with fragmented DNA.  相似文献   

Beneficial role of melatonin in protecting mammalian gametes and embryos from oxidative damage     

Yun-wei PANG  Xiao-long JIANG  Shan-jiang ZHAO  Zi-qiang HUANG  Hua-bin ZHU 《农业科学学报》2018,17(10):2320-2335

Mammalian gametes and embryos are particularly vulnerable to oxidative stress-induced damage, which is mainly caused by reactive oxygen species (ROS) originating from normal metabolism and/or the external environment. Several researchers have implicated the role of oxidative stress in the activation of apoptosis, causing peroxidative damage to sperms/oocytes and inducing embryo fragmentation, arrest, or demise. Melatonin is a tryptophan derivative that is known for its powerful free radical-scavenging activity and broad-spectrum antioxidant property. Numerous studies have shown that melatonin and its metabolic derivatives can sequentially detoxify ROS in an antioxidant cascade, and modulate various antioxidant enzymes via its receptors to prevent radical-mediated damage. The identification of melatonin receptors in cumulus/granulosa cells, oocytes, and epididymal tissues implies that melatonin has protective actions on gametes and embryos. Enriching the semen extender or culture medium with melatonin significantly benefits sperm characteristics, improves oocyte maturation potential and quality, and enhances the developmental competence of preimplantation embryos. Certainly, further comparative studies are needed to show the unique antioxidant role and the advantage of melatonin in this field. This review summarizes the harmful effects of ROS and the beneficial role of melatonin against oxidative damage of gametes and embryos.  相似文献   

高压静电场对鸡精子受精率及其胚胎发育影响的研究   总被引：1，自引：0，他引：1  

王全喜  布和 《内蒙古农业大学学报(自然科学版)》2000,21(2):24-27

选用５１８只母鸡、２０只公鸡（海赛克斯褐父父母代鸡）,公鸡精液用高压静电场处理后人工受精。分析研究高压静电场对鸡精子受精率及其胚胎发育的影响。结果表明用一定剂同压静电场处理鸡精液能提高精子受精率、缩短出雏持续时间,但剂量过大时降低受精率、增加鸡早期胚胎死亡,从而又影响孵化率。本次实验表明６００ｋｖ／ｍ＊１５ｍｉｎ剂量的高压静电场对鸡精子受精率和胚胎孵化率具有显著的提高作用。即受精率提高６．５６个百分点,孵化率提高１０．３３个百分点。这对养殖业生产有着积极的意义。  相似文献   

猪精液中式稀释液筛选研究   总被引：5，自引：0，他引：5  

张兆旺  吴永孝 《中国农业科学》2000,33(2):104-106

为克服基层单位设备不足 ,药品不全、称量不准、配制工序繁琐等精液稀释液的缺点 ,继吴永孝等用全天然中草药制备牛冻精解冻液获得成功后〔1〕,我们开展了中式精液稀释液的研究课题 ,现将有关猪精液中式稀释液的研究结果报道如下。1　材料与方法1 .1　试验组中式稀释液的制备供试的 4个方剂依次编号为 1号、2号、3号和 4号 ,均由市售常用中药组成。溶剂是天然饮用水。为了验证天然中草药复杂成分的整体效应和便于在生产中应用 ,本试验所用中药一律不用“提纯”工序 ,只按中药的常用水剂方式制备。1 .2　对照液对照液配方为葡萄糖 5g、柠檬酸…  相似文献   

中国对虾卵水的特性和精子的应答   总被引：6，自引：0，他引：6  

孙修涛 《水产学报》2000,24(1):1-6

研究了中国对虾卵水的采集、保存方法、紫外吸收特性和有效期等方面的内容,并应用卵水研究了交尾期虾、雌虾及产卵期雌虾精荚或纳精囊中精子对卵水的应答开始时间、必要的反应时间等响应卵水的时间特性以及精子的存活期等。结果表明,卵水经液氮保存或先经液氮后转入普通冰柜保持冻结状态７个月后诱导精子激活效力无显著差异。精子在自然温度（１０℃）普通海水中可在１０ｈ内保持对卵水的响应能力。交尾期精子最初响应卵水有５￣１  相似文献   

不同活性化物质对精子运动性及受精率的影响   总被引：2，自引：0，他引：2  

张明新  梅东林  菊地武  夏彤 《扬州大学学报(农业与生命科学版)》2005,26(2):32-36

将用于体外受精的种公牛精液,分别添加咖啡因、茶碱和己酮可可碱3种黄嘌呤活性化物质,探讨其对精子运动性及受精率的影响。结果表明：精子的运动性,以添加己酮可可碱处理的较高,随着时间的推移,添加茶碱处理的相对较高,但试验组与对照组差异不显著。受精率除添加己酮可可碱处理与对照组有显著差异外,其他组间均无显著差异。不同种公牛的精子运动性存在差别,但受精率无显著差异。  相似文献   

SPERMATIC GRANULOMA IN THE RAM: DIAGNOSIS BY ULTRASONOGRAPHY AND SEMEN CHARACTERISTICS     

Fikret  Karaca  Melih  Aksoy  Abdullah  Kaya  Mehmet B.  Ataman  Tevfik  Tekeli 《Veterinary radiology & ultrasound》1999,40(4):402-406

Ultrasonographic appearance of the testis and epididymis, and seminal characteristics, with regard to localization of spermatic granuloma were studied. In rams with spermatic granuloma (n = 9), diagnosed by clinical or ultrasonographic examinations before histopathological confirmation, localization of each lesion was recorded. Epididymal granulomas, caput and cauda, were seen as anechoic or hyperechoic areas with a distinct margin with or without a hyperechoic capsule. Granulomas in the testis were microscopic and, therefore, could not be detected by ultrasonography. Enlargement in the mediastinum testis was detected in all rams when there were granulomas in the caput. Heterogeneous testis parenchyma invaded by numerous hyperechoic foci, representing testicular degeneration, was associated with granulomas both in the epididymis and testis. Ultrasonographic appearance of the lesions did not differ with regard to season. Seminal characteristics varied between rams. It was concluded that ultrasonographic evaluation may give valuable information in the diagnosis of sperm granuloma in the ram.  相似文献   

甲基黄嘌呤类生物碱在精子体外处理中的应用     

林金杏  阎萍 《黑龙江动物繁殖》2006,14(4):7-9

在人工授精及体外受精过程中,需要对精子进行活化处理.甲基黄嘌呤类生物碱是目前日益受到重视的精子体外处理剂.本文主要阐述了茶碱、己酮可可碱和咖啡因三种甲基黄嘌呤类生物碱的理化性质和在精液处理过程中对精子的受精力影响及其作用机理.  相似文献   

年龄对蓝白花种公牛冻精质量的影响     

王业炳  王业荣  李艳梅  赵凌平 《中国牛业科学》2006,32(6):9-11

以16头蓝白花种公牛为对象,对其采精适宜年龄及不同年龄对冻精质量的影响进行了研究。结果表明,蓝白花种公牛最初采精以20月龄为宜,3-6岁精液质量最好,7岁后精液质量呈下降趋势。  相似文献   

犬精液液态保存稀释液及温度筛选试验   总被引：4，自引：0，他引：4  

李彩霞  魏庆信  郑新民  崔岘  魏雁  陈永昌 《黑龙江动物繁殖》2004,12(2):5-7

探讨了8种稀释液在5℃、10℃、15℃和20℃下保存犬精液的效果。结果表明:15℃为犬精液液态保存的较适温度,3号液为优等液,其中3号液在15℃精子的存活时间为105±10h,与对照液在15℃的保存效果差异极显著(p<0.01)。2、4、5号液是良等液,在15℃精子的存活时间是64±8h,与对照液在15℃的保存效果差异显著(p<0.05)。中等液为6号液,在15℃、20℃保存时与对照液没有统计学上的差异(p<0.05),在其他2种温度保存时不及对照液。7、8号液为差等液,在15℃保存时与对照液差异不显著(p<0.05),在其他3种温度时不及对照液。  相似文献