养殖刺参溃疡病杀鲑气单胞菌的分离、致病性及胞外产物特性分析   总被引：3，自引：0，他引：3       下载免费PDF全文

杨嘉龙  周丽  邢婧  绳秀珍  战文斌 《中国水产科学》2007,14(6):981-989

从患溃疡病的养殖刺参(Apostichopus japonicus)病灶处分离出1株优势菌H1,以浸浴、创伤浸浴、体腔注射和体壁肌肉注射等方式进行感染实验,证实菌株H1为养殖刺参溃疡病病原菌,并证明该菌通过体表创伤侵入的方式感染刺参,以创伤浸浴和体壁肌肉注射感染的LD50(半数致死量)分别为2.26×107CFU/尾和1.80×107CFU/尾。经形态学观察、生理生化特性分析和mini API系统鉴定,确定菌株H1为杀鲑气单胞菌杀日本鲑亚种(Aeromonas salmonicida ma-soucida)。提取菌株H1的胞外产物(ECP)进行致病性实验,结果表明ECP可导致刺参死亡,其对刺参的LD50为5.24μg蛋白/g体质量。H1-ECP具有酪蛋白酶、明胶蛋白酶、几丁质酶和淀粉酶活性,并具有溶血素活性;对底物偶氮酪蛋白(Azocasin)作用的酶比活力可达到674.5活力单位/mg蛋白,最适作用温度为50℃;对热不稳定,70℃作用30 min时,酪蛋白酶活性降到0;100℃作用30 min,ECP对刺参的毒性消失;ECP酶活可被10 mmol/L EDTA完全抑制,可被5 mmol/L PMSF抑制98.8%,Ca2 和Mg2 可使酶活性分别提高约9%和4%。结论认为,该病原菌通过体表创伤侵入方式感染宿主刺参,菌株H1胞外产物是其对刺参致病的因子之一。  相似文献   

Characterization of MicroRNA* Species in Peking Duck Skin     

Li ZHANG  Xiu-juan XIE  Shan-gang JIA  Shu-dai LIN  Li-long AN  Wen LUO  Xin-zheng JIA  Qing-hua NIE  Xi-quan ZHANG 《农业科学学报》2013,12(9):1614-1619

A substantial fraction of miRNA* species are conserved in animals and can repress activities of target genes. This study aims to investigate the miRNA* species in duck skin by using Solexa sequencing. We obtained a total of 96 miRNA* species in two skin small RNA libraries and identified 56 miRNA/miRNA* (miR/miR*) pairs. Nucleotide bias of miRNA* indicated that the priority was C>A>U>G for the first nucleotide and U>C>A>G for the last nucleotide. Comparison analyses showed that 3′-U accounted for a higher proportion in the 56 miR/miR* pairs. Among the top 20 expressed miRNA* species, 17 were shared by two libraries and most of the miRNA* species were highly conservative, especially in the “seed region”. miR-199a* were expressed highly in our samples, which was also previously shown abundant in mouse hair follicle. Furthermore, four miRNA* species were predicted to target their genes in signal pathways of feather follicle development and feather morphogenesis despite very low levels.  相似文献   

H-Y抗血清的制备及检测研究   总被引：1，自引：0，他引：1  

杭苏琴  牛树理 《畜牧与兽医》1999,31(4):15-16

选用８ ～１２ 周龄纯系的 Ｂ Ａ Ｌ Ｂ／ Ｃ 母鼠， 用同日龄的 Ｂ Ａ Ｌ Ｂ／ Ｃ 公鼠采取皮肤移植和脾细胞注射２ 种方式免疫， 制备 Ｈ Ｙ 抗血清， 经精子微量细胞毒性试验， 筛选抗血清。皮肤移植５ 只小鼠中只有１ 只产生较好的抗血清， 脾细胞注射的４ 只小鼠都能产生较好的抗血清， 产生抗血清的小鼠占５５ ％ 。抗血清与昆明鼠正常８ｃｅｌｌ 早期桑椹胚培养５ ～６ 、１８ 和２４ ｈ 后， 退化率达４３３ ％ ， 经 Ｘ２ 检验差异不显著（ Ｐ ＞ ００５） ， 符合自然性比例。  相似文献   

不同处理条件下柔鱼胴体特性的变化     

张冬梅  俞鲁礼 《上海海洋大学学报》1999,(2):149-153

以北太平洋的柔鱼为原料，研究柔鱼的胴体在2℃、50℃和100℃的处理条件下，重量、水分含量及总氮量的变化。通过研究发现，去皮柔鱼胴体在2℃冷水中漂洗时，水分含量的变化是引起重量变化的主要原因，而在漂洗过程中总氮量变化不明显；带皮柔鱼胴体在50℃热水中漂洗时，漂洗水量的增加比时间的延长更有利于防止营养成分的损失；100℃漂烫时，为防止脱水及营养成分的流失，应控制在4分钟内完成。  相似文献   

中华富铁康900对断奶仔猪的饲喂效果试验     

《安全饲料添加剂产业化技术研究与应用》课题组 《四川畜牧兽医》2004,31(3):26-26,28

试验选用体重约8kg的三元杂交(杜长大)猪60头,随机分成3组,每组2个重复,分别饲喂含不同剂量中华富铁康900的饲粮,研究中华富铁康900对仔猪生产性能、肤色的影响。结果表明,以中华富铁康900取代饲粮中部分硫酸亚铁,显著提高仔猪平均日增重(P<0.05),显著降低料重比(P<0.05),明显改善仔猪肤色。根据试验效果,仔猪饲粮中中华富铁康900的添加量为2×10-4～4×10-4。  相似文献   

Changes in meat texture of three varieties of squid in the early stage of cold storage     

MIEKO KAGAWA  MISUZU MATSUMOTO  CHIE YONEDA  TOMIKO MITSUHASHI  KEIKO HATAE 《Fisheries Science》2002,68(4):783-792

ABSTRACT: The present study examined the changes in texture and protein components during cold storage of different squid varieties. Raw oval squid, Japanese common squid and arrow squid were sliced fresh and the muscles were stored at 4°C for 0, 4, 8, 12, 18, 24, 48 and 120 h. The rheological measurements, protein components and amounts of collagen were examined. The adhesiveness of each squid increased significantly in the early stage of cold storage. In all varieties, penetration decreased at 4 h, which is considered to be rigor mortis, then increased. The amounts of total collagen, 20°C water-soluble collagen and 70°C water-soluble collagen did not change significantly in each variety during cold storage. Sodium dodecylsulfate–polyacrylamide gel electrophoresis (SDS-PAGE) pattern showed that the 580 kDa component gradually disappeared up to 48 h. The correlations between the amounts of 580 kDa component and adhesiveness or firmness were high. Models of fit based on chemical kinetics accurately expressed the behavior of adhesiveness, firmness and penetration showing that 63.2% of adhesiveness changes occurred in 13–19 h and that 63.2% of firmness changes occurred in 18–24 h.  相似文献   

Studies on the Pathogenesis of Shimao Zheng (Fleece-eating) in Sheep and Goats     

Youde  Huang  Huaitao  Chen 《Veterinary research communications》2001,25(8):631-640

Research on the disease called shimao zheng, which had resulted in widespread fleece-eating and shedding by sheep and goats in the Haizi area of Akesa County of Gansu Province of China, was carried out by both laboratory studies and pathological observations. There was a marked deficiency of sulfur in the wool and tissues of the affected animals. The pathological changes were mainly loss of fleece, exposure of the skin, keratinization of the epidermal cells, narrowing and reduced numbers of the fleece follicles, and fewer sebaceous glands and sweat glands. Histologically, there was atrophy of the striated and cardiac muscles and of the epithelium of the convoluted tubules of the kidney, together with a glomerulonephritis. The disease was considered to be a local nutritional and metabolic disorder, probably mainly caused by sulfur deficiency.  相似文献   

几种鸡胚原代细胞的制备方法   总被引：1，自引：0，他引：1  

缪从容  吴贤福 《中国兽药杂志》1997,31(1):14-16

在病毒分离、繁殖，中和试验及药物毒理试验中，细胞培养是经常被选用的技术。本文描述通过选择不同日龄的鸡胚，分别对皮肤上皮细胞、肝细胞和肾细胞进行了组织培养，确定了生长所需的培养液要求。认为１２日龄鸡胚适于皮肤上皮细胞的制备，１７～２０日龄鸡胚适合于肝细胞、肾细胞的制备，并确定了细胞生长的ｐＨ条件。  相似文献   

被毛突变无毛小鼠皮肤及毛结构观察   总被引：6，自引：1，他引：5  

遇透玲  李善如 《中国兽医学报》1996,16(3):299-301

对国内首次发现并培育成群的ＢＡＬＢ／ｃ突变小鼠的３种表型（无毛、全毛、中间态）的皮肤组织及毛结构进行了观察。结果表明，无毛小鼠仅具有非常短（１～２ｍｍ）、细且弯曲的绒毛，其毛于中心为空泡状，缺乏髓质。无毛小鼠表皮、真皮、皮下组织结构基本与全毛、中间态小鼠相同，但其毛生长周期不健全；毛球位置浅表，数目少，排列不规则；皮脂腺异常增生。推测其被毛结构异常是由毛自身结构异常所致。该小鼠外观似裸鼠，能在普通条件下生长繁殖，可望成为某些皮肤病等研究的动物模型。  相似文献   

柔鱼不同部位的呈味物质含量比较     

石建高  钟文珠  陶礼民 《上海海洋大学学报》2002,(1):58-61

将柔鱼可食部分分成胴体，鳍和头足三部分，用80％乙醇抽提法分别对其其味物质作提取试验，测定了不同部位的原料和抽提物中主要呈味物质的含量，并对其中的甜菜碱，氨基酸及无机离子K^ ,Na^ ,Ca^2 ,Fe^2 ,Zn^2 ,PO4^3-,Cl^-等作比较研究。结果表明：无论是原料还是抽提物中，胴体部分甜菜碱和氨基酸含量都最高，而鳍和头足中的含量相对偏低且相差不大；无机离子在胴体，头足和鳍中的含量则依次减少，抽提物中牛磺酸的含量胴体比鳍和头足低。此外，对柔鱼中呈味成分的应用进行了可行性的初步探索。  相似文献