Comparative pathogenicity study of ten different betanodavirus strains in experimentally infected European sea bass,Dicentrarchus labrax (L.)     

N Vendramin  A Toffan  M Mancin  E Cappellozza  V Panzarin  G Bovo  G Cattoli  I Capua  C Terregino 《Journal of fish diseases》2014,37(4):371-383

Viral encephalopathy and retinopathy (VER), otherwise known as viral nervous necrosis (VNN), is a severe pathological condition caused by RNA viruses belonging to the Nodaviridae family, genus Betanodavirus. The disease, described in more than 50 fish species worldwide, is considered as the most serious viral threat affecting marine farmed species in the Mediterranean region, thus representing one of the bottlenecks for further development of the aquaculture industry. To date, four different genotypes have been identified, namely red‐spotted grouper nervous necrosis virus (RGNNV), striped jack nervous necrosis virus (SJNNV), tiger puffer nervous necrosis virus and barfin flounder nervous necrosis virus, with the RGNNV genotype appearing as the most widespread in the Mediterranean region, although SJNNV‐type strains and reassortant viruses have also been reported. The existence of these genetically different strains could be the reason for the differences in mortality observed in the field. However, very little experimental data are available on the pathogenicity of these viruses in farmed fish. Therefore, in this study, the pathogenicity of 10 isolates has been assessed with an in vivo trial. The investigation was conducted using the European sea bass, the first target fish species for the disease in the Mediterranean basin. Naive fish were challenged by immersion and clinical signs and mortality were recorded for 68 days; furthermore, samples collected at selected time points were analysed to evaluate the development of the infection. Finally, survivors were weighed to estimate the growth reduction. The statistically supported results obtained in this study demonstrated different pathogenicity patterns, underlined the potential risk represented by different strains in the transmission of the infection to highly susceptible species and highlighted the indirect damage caused by a clinical outbreak of VER/VNN.  相似文献   

Seasonal Changes in Rigor Development and Flesh Texture of Wild Japanese Sea Bass (Lateolabrax japonicus)     

Teruo Nakayama  Keiko Hatae  Midori Kasai  Atsushi Ooi 《Journal Of Aquatic Food Product Technology》2017,26(5):578-592

The season for finding the firmest sashimi made from wild Japanese sea bass was investigated. Flesh rigor tension and contraction were measured by a “new” device with isometric (muscle length kept constant) and isotonic (balance beam moves freely) transducers. Both flesh rigor tension and contraction peaks appeared at the same postmortem times, with attainment of ultimate pH and adenosine triphosphate/inosine monophosphate ratio in four seasons. Seasonal seawater temperature correlated negatively to seasonal maximum rigor contraction and positively to seasonal flesh breaking strength at 72 h postmortem. After rigor peak tension attainment, the tension relaxation was much lower than that of other wild fish species, especially in summer. The sea bass muscle resisted structure disruption in rigor isometric tension generation. Wild sea bass sashimi was firmest in breaking strength in summer.  相似文献   

淡水池塘主养鲈试验     

薄学峰  张汉珍 《齐鲁渔业》1998,15(6):26-27

报道了首次利用淡水鱼塘,主养鲈兼养鲢,鲤,草鱼等试验,每亩放苗５１５尾,鲈占放养总重的７１．７％;主喂冷冻青鳞鱼,约占７７．７％,辅喂人工配饵;前期只充渗漏水,中后期透明度小于３０ｃｍ时,大量换水。结果：共产商品鱼８８０２ｋｇ,其中鲈３７４０ｋｇ,折合亩产１２４．７ｋｇ,成活率８９％。总产值１２８０４６．８地,纯利７６９６９．８地,平均亩纯利２５６５元,投入产出比１：２．５。  相似文献   

Abnormalities in the juvenile stage of sea bass (Dicentrarchus labrax L.) reared at different temperatures: types, prevalence and effect on growth     

I. Abdel  E. Abellán  O. López-Albors  P. Valdés  M.J. Nortes  A. García-Alcázar 《Aquaculture International》2005,12(6):523-538

Osteological malformations are always considered an important problem in intensive aquaculture. This work studies the effect of rearing temperature on malformations in sea bass, Dicentrarchus labrax. To this end, two batches of the species were subjected to the following incubation/cultivation temperatures: 15 °C/natural and 19/19 °C, from fertilization until 190 days after hatching. The different malformations were studied in 1643 juveniles at 190 days and classified by categories. The Chi-square statistic was calculated to verify the relationship between the presence of anomalies and the application of different temperatures. The percentage of anomalies observed in individuals reared at a high temperature (19/19 °C) was 66.44%. In both temperature systems, opercular malformations were those which caused a greater delay in growth. The results found indicated that temperature played a very important role in the development of deformations, which may be of interest from the viewpoint of aquaculture.  相似文献   

Nocturnal feeding reduces sea bass (Dicentrarchus labrax, L.) pellet-catching ability     

V. C. Rubio  F. J. Snchez-Vzquez  J. A. Madrid 《Aquaculture (Amsterdam, Netherlands)》2003,220(1-4):697-705

European sea bass exhibits a feeding rhythm with seasonal phase inversions: nocturnal feeding is predominant in winter, and diurnal feeding during the rest of the year. This explains the increased growth of sea bass fed at night during the winter months, but to fully assess such feeding practices, it is important to monitor food-catching efficiency in the dark. To evaluate the influence of nocturnal feeding on pellet-capture efficiency under laboratory conditions, we developed an infrared pellet detector linked to on-line recording with a microcomputer. Feed waste was recorded for eight groups of four sea bass (54.6±15.8 g body weight) fed either diurnal or nocturnal meals with long (>60 s) or short (<20 s) feed availability times. With short feed availability times, food-catching efficiency in complete darkness (0 lx) was reduced to 78.6%, compared to 93.5% in diurnally fed fish. This was confirmed in a second experiment, performed during winter, involving 4000 sea bass in four sea cages (1000 per cage) provided with self-feeders and pellets detectors. Feed waste amounted to less than 13% of the food distributed, but nocturnal demands (29% of total feed demands) were accompanied by higher levels of uneaten pellets (68% of the total feed waste) than diurnal demand. This suggests that if the aim is to reduce feed waste, nocturnal feeding should be restricted in sea cages.  相似文献   

A preliminary study on the utilization of hazelnut meal as a substitute for fish meal in diets of European sea bass (Dicentrarchus labrax L.)     

Y&#;lmaz Emre  Hüseyin Sevgili  & Murat &#;anl&#; 《Aquaculture Research》2008,39(3):324-328

  相似文献   

大口黑鲈肌肉生长抑制素基因单核苷酸多态性位点的筛选及其与生长性状关联性分析   总被引：4，自引：2，他引：4  

于凌云  白俊杰  樊佳佳  李小慧  叶星 《水产学报》2010,34(6):665-671

采用PCR-SSCP和PCR-RFLP技术对大口黑鲈肌肉生长抑制素(myostatin,MSTN)基因全序列进行了SNPs位点筛选和分型,共筛选到2个单核苷酸多态性(SNPs)位点(C-1453T和T+33C),其中C-1453T位于启动子E8 box和Octamer(＋)调控元件之间的区域,T+33C的突变位于第一外显子区域,属于同义突变,氨基酸没有发生变化;利用一般线性模型分析单标记位点与大口黑鲈生长性状(体重、体长、体高、体宽和眼间距)相关性,均未达到显著水平(P>0.05)。将2个SNPs位点不同基因型组合成6种双倍型(去掉频率小于3%的组合),关联分析表明,双倍型D2在体重、体长、体高、体宽和眼间距的均值均高于其它双倍型,而双倍型D5在体重、体长、体高、体宽和眼间距的均值均低于其它双倍型,双倍型D2与D5之间在5个主要生长性状均存在差异显著(P＜0.05),推测双倍型D2对生长性状起正相关,而双倍型D5与大口黑鲈的生长性状呈负相关,因此推断MSTN基因突变位点双倍型D2与D5可作为大口黑鲈生长性状的两个标记位点,用其分子标记位点来辅助大口黑鲈育种工作以期加快育种进程。  相似文献   

DEM空间尺度对可照时数模拟结果的影响 ——以浙江省仙居县为例     

李军  王超  赵玉竹 《中国农业气象》2019,40(4):250

以浙江省仙居县为实验区,基于4种不同空间分辨率DEM提取相关地形因子,结合可照时数分布式模型,利用数字地形分析和空间数据叠置分析等方法,模拟1月和7月4种空间分辨率下的可照时数,并定量分析DEM尺度效应对模拟结果的影响。结果表明：（1）模拟结果的空间异质性随分辨率减小而减小,其平均值逐渐增加,且1月增幅大于7月,最大值随分辨率的变化不大,而最小值差异较大,标准差逐渐减小。（2）受海拔和地形遮蔽影响,平地和山脊处可照时数最多,海拔200-400m区间最少,400-1100m区域可照时数随海拔增加而增加。以10m分辨率结果为参照,30m、90m和900m分辨率下的差值随分辨率减小而增加,海拔<100m处差值最小,700-900m区域差值最大,1月日均多算0.7、1.4和2.9h,7月日均多算0.5、0.9和2.3h。（3）坡度0-55°范围内,可照时数随坡度增加而减少,30m、90m和900m分辨率与10m分辨率的差值随分辨率降低而增加,且最小差值均在<5°区域,最大差值在不同坡度等级,1月日均多算2.1、1.8和1.7h,而7月日均多算0.3、0.6和1.2h。（4）受太阳高度角和方位角影响,可照时数在南坡-北坡间的差异较大,东南-西南坡、东坡-西坡以及东北-西北坡之间差异较小,30m、90m和900m分辨率与10m分辨率的差值随分辨率降低而增加,在偏北坡,1月差值大于7月,最大差值在1月的北坡上,日均多算1.4、2.5和4.8h,在偏南坡上,1月差值小于7月,最大差值在7月的南坡或西南坡上,日均多算0.5、0.9和2.1h。  相似文献   

通辽市气象因子对红薯产量的影响分析     

董佳蕊 《中国农学通报》2022,38(24):75-79

研究通辽市气象因子对红薯种植产量的影响,旨在为通辽市农业种植结构调整提供科学依据。以红薯推广种植基地的种植资料和气象观测资料为基础数据,对气象因子与红薯产量的相关性进行分析。结果表明,积温、日照时数与红薯产量相关显著,相关系数分别为0.741和0.837,日平均气温和降水量与红薯产量未达到显著水平。除发根返青期外,其他发育期与全生育期的积温变化特征近似,相关系数均在0.788以上;日照时数年际变化与全生育期变化特征一致;仅薯块膨大期与全生育期降水量和日平均气温的年际变化相关显著,相关系数分别为0.933和0.936。热量和光照条件对产量影响相对较大,是红薯种植的限制因素,降水对产量影响不明显。  相似文献   

1961—2015年安徽省春季日照时数变化特征     

伍晓玲  张脉惠  罗艳 《中国农学通报》2017,33(26):83-87

为了研究安徽省春季日照时数变化特征,为合理安排农事提供依据,使用近55a(1961—2015年)安徽省春季78个气象台站逐日日照时数资料,利用相关分析、线性趋势分析、Mann-Kendall以及Morlet小波变换分析等方法对安徽省春季日照时数进行了分析,结果表明：安徽省春季多年平均日照时数在381.55—626.75h之间,呈北多南少的空间分布特征;春季日照时数年际变化明显,日照时数最多年为最少年的1.7倍。在近55a中,日照时数整体上呈现出先减后增的变化趋势;在各个年代中日照时数周期存在差异;在21世纪初日照时数由减少转为增加,属于突变现象;安徽省春季日照时数变化趋势存在明显的空间差异,淮北主要以减少趋势为主,江淮之间增减参半,沿江江南则主要表现为增加趋势。  相似文献