卷丹保健饮料的研制     

钟海雁  李忠海  王纯荣  农全畅 《中南林业科技大学学报(自然科学版)》2003,23(1):28-31

根据卷丹富含淀粉、蛋白质的特点 ,以干片及制淀粉所得的清汁为原料 ,进行了保健饮料的加工工艺研究 .结果表明 :采用 0 .0 2 %的黄原胶、0 .1%的 CMC—Na和 0 .1%的海藻酸钠作为稳定剂 ,使卷丹奶有较好的稳定性 ;卷丹奶饮料最佳配方为 :1∶ 9的卷丹与水混合 ,6%的白砂糖 ,4%的脱脂乳 ,0 .1%的柠檬酸 .清汁饮料的最佳配方为 :6%的白砂糖 ,0 .1%的柠檬酸  相似文献   

虾塘养殖对红树林沉积物和间隙水理化性质的影响     

覃盈盈  罗万次  梁铭忠  魏明月  高长颢  郑海雷 《农业现代化研究》2021,42(2):357-366

红树林湿地在近海水体营养控制和营养盐循环过程中扮演着重要的角色。沉积物、水体和植被相互作用共同维持其生态功能的运转,了解虾塘养殖对林下沉积物和间隙水养分特征影响对评价红树林生态功能具有重要指导意义。本研究以受虾塘养殖废水排放不同影响程度的红树林（无排放区、直排区、修复区）为研究样地,通过不同生境下沉积物与间隙水养分特征的分析,探讨虾塘养殖对他们的影响。结果如下：1）受虾塘养殖废水排放影响区中（直排区、修复区）红树林下沉积物TN与OM、TP呈正相关关系,虾塘养殖废水直排对林下沉积物具有明显酸化作用;2)NH4+-N是红树林下间隙水可溶性无机氮（DIN）的主要组成形式,无排放区沉积物还原性较强,更利于NH4+-N累积在间隙水中;NO3--N与DIN呈极显著相关,虾塘养殖废水排放增强了NO3--N、NO2--N和DIN的累积效应。研究结果表明沉积物和间隙水的理化性质可作为评价虾塘养殖区边邻红树林生境变化规律的定量指标之一。  相似文献   

致对虾肝胰腺坏死病副溶血弧菌荧光定量PCR检测方法的建立     

陈平亚  张亮亮  吴少荣  黄纪徽  王绥家  吴山楠 《广东农业科学》2021,48(11):119-125

【目的】建立一种对虾急性肝胰腺坏死病（Acute Hepatopancreatic Necrosis Disease）病原菌副溶血弧菌（Vibrio parahaemolyticus, VpAHPND）荧光定量 PCR 检测方法,对该病进行快速检测。【方法】根据副溶血弧菌基因保守序列设计特异性引物和探针,经优化反应体系及条件,建立检测 VpAHPND 的荧光定量 PCR 方法;以重组质粒为标准品制作标准曲线,并进行特异性、敏感性、重复性及临床应用试验。【结果】该方法定量范围宽,起始模板浓度范围为 2.3×101 ~2.3×107 拷贝 /μL 时,标准曲线具有良好的线性关系;最低检测限为 2.3 拷贝/μL,与对虾其他病原菌及病毒无交叉反应,重复试验变异系数为 0.45%~0.69%,可在 1 h 内完成样品检测;对临床对虾样品的检测结果表明,该方法与世界动物卫生组织（OIE）推荐的套式 PCR 法检测结果一致。【结论】应用 TaqMan 探针建立的检测 VpAHPND 的荧光定量 PCR 方法具有灵敏度高、特异性强、重复性好的特点,可有效用于临床对虾样品的 VpAHPND 检测。  相似文献   

卤虫卵化学成分的分离与分析   总被引：1，自引：0，他引：1       下载免费PDF全文

陶海荣  阮栋梁  戴宇容  李和 《水产科技情报》2004,31(2):59-61

本试验对卤虫卵的化学成分进行分离和分析。卤虫卵的含油量为7．1％，油中不皂化物的含量为4．9％，主要为胆甾醇。脂肪酸中含有较大量的油酸、棕榈酸、棕榈油酸、花生四烯酸和二十碳五烯酸。卤虫卵含蛋白质为20．9％，还含有多种氨基酸。  相似文献   

The effects of variation in feed protein level on the culture of white shrimp, Litopenaeus vannamei (Boone) in low-water exchange experimental ponds     

L R Martinez-Cordova  A Campaña-Torres  & M A Porchas-Cornejo 《Aquaculture Research》2002,33(12):995-998

The present study was conducted to evaluate the effect of varying dietary protein level on pond water quality and production parameters of white shrimp Litopenaeus vannamei (Boone). Experimental units consisted of nine 400‐m² earthen ponds with a low water exchange. Two treatments were tested: treatment HP consisted of shrimp fed a high‐protein diet (40%) during the whole grow‐out, and treatment LP consisted of the use of a low‐protein diet for the complete farming period. No differences on any of the water quality parameters were observed among treatments. Excellent survival (over 85%) and feed conversion ratios (around 1.6), and acceptable growth (over 12 g) and biomass (from 1721 to 1793 kg ha^?1) were recorded in all experimental ponds. No significant differences in any of the production parameters were found among treatment groups.  相似文献   

水温和盐度对南美白对虾幼虾能量收支的影响   总被引：13，自引：0，他引：13  

王吉桥 《水产学报》2004,28(2):161-166

用室内生态实验法测定了南美白对虾幼虾在不同盐度(5,15或25)和水温(20～23℃,25℃,30℃或33℃)下摄食水丝蚓的能量收支方程。在盐度5～25时,随盐度降低,对虾的能量转换效率升高,同化率降低。在盐度为5时,K1最高(29.02%),同化率最低(77.89%)。对虾具补偿机制来补偿调节渗透压的能耗。盐度对同化率的影响,主要是通过影响呼吸代谢实现的。对虾的特定生长率(y)(%·d-1)随水温(X)(℃)上升而增加,其关系式为:y=0.0168LnX 0.0831(R2=0.9324)。对虾在较高温度下生长快,主要是增加绝对摄食量,提高吸收效率,减少粪便排泄量。在相同温度下,变温时对虾的摄食量高于恒温时。  相似文献   

Dietary potassium requirement of juvenile grass shrimp Penaeus monodon   总被引：2，自引：0，他引：2  

Shi-Yen Shiau  Jia-Fen Hsieh 《Fisheries Science》2001,67(4):592-595

ABSTRACT: A feeding trial was conducted to estimate the minimal dietary potassium (K) requirement for juvenile grass shrimp Penaeus monodon . Purified diets with seven levels (0, 0.3, 0.6, 0.9, 1.2, 1.5, 2.0 g/100 g) of supplemental potassium were fed to P. monodon (mean initial weight 0.75 ± 0.01 g) for 8 weeks. Each diet was fed to three replicate groups of shrimp. The rearing water contained 360 mg/L potassium. Weight gain and protein efficiency ratio (PER) of shrimp improved as dietary potassium supplementation level increased up to 1.5 g K/100 g diet and thereafter declined significantly ( P < 0.05). Feed efficiency (FE) of shrimp also showed a similar trend. However, whole body K concentration of the shrimp kept an increasing trend up to 2.0 g K/100 g diet. Analysis of the weight gain (percent) and PER of the shrimp by polynomial regression indicate that the minimal dietary potassium requirement in growing P. monodon is approximately 1.2 g/100 g.  相似文献   

Effects of crushed conspecifics on growth and survival of Penaeus monodon Fabricius post larvae     

Bui Tui Nga  Miquel Lürling  Edwin Peeters  Rudil Roijackers  Marten Scheffer  & Truong Trong Nghia 《Aquaculture Research》2006,37(3):224-232

In the present study, the hypothesis tested was that Penaeus monodon post‐larvae (PL) experience lower growth when exposed to crushed conspecifics, which was achieved by exposing individual P. monodon PL with abundant food for 4 weeks to a gradient from 0 to 100 crushed conspecific PL L^?1. Both dry weight (48.5±7.2 mg) and body size (28.0±1.3 mm) of animals exposed to 1 macerated PL L^?1 were significantly (P≤0.011) higher than those of animals in treatments with 0, 5 and 10 crushed PL L^?1 (average over treatments: 25.6±3.2 mg; 23.4±0.5 mm). All animals died within 1 week when exposed to 70 and 100 crushed PL L^?1, and within 3–4 weeks when exposed to 50 and 30 crushed PL L^?1. Exposure time affected mortality and it appeared that LC₅₀ values decreased from 60 to 13 crushed PL L^?1 from 1 to 4 weeks' exposure. Survival of P. monodon PL was negatively correlated to pH, biological oxygen demand, ammonia and nitrate. In conclusion, low dose of crushed conspecifics has a stimulatory effect on P. monodon PL, as larvae were heavier and larger, while high doses cause high mortality.  相似文献   

池塘闭路循环水养虾的研究     

徐明起  朱伯清 《海洋水产研究》1993,(14):71-77

阐述了池塘闭路循环水水质的变化及养虾效果,循环水开机4h后,氮氮和化学耗氧量,分别降低了11.09％和17.35％,下层水的溶解氧含量比对照池提高了1.22ml/L,并使上、下水层的水温和pH值趋向平衡。生产应用试验证明,使用闭路循环水比对照池的成活率提高了7.78％,降低投饵系数12.18％,提高产量19.21％,每公顷增加效益6354元。  相似文献   

对虾皮下及造血组织坏死杆状病毒的精细结构、核酸、多肽及血清学研究   总被引：26，自引：3，他引：26  

黄  于佳  宋晓玲  孔杰  杨丛海 《海洋水产研究》1995,16(1):1-23

对纯化的对虾皮下及造血组织坏死杆状病毒（ＨＨＮＢＶ）进行了超微结构、紫外吸收、核酸类型及多肽ＳＤＳ－ＰＡＧＥ的研究。同时用单克隆抗体对ＨＨＮＢＶ的两分离株和ＭＢＶ进行了血清学比较研究。用ＴＭＶ作为内标定物测得病毒粒子大小为１５０ｎｍ×４５０ｎｍ，其衣壳为两个帽状物端夹１３圈螺旋对称的园柱体结构。病毒在２６０ｎｍ处吸光系数为０．１１７ｍｇ／ｍＬ／ＯＤ２６０。病毒核酸为双链ＤＮＡ，分子量大于３５×１０６ｄ。病毒囊膜有１２种主要多肽。病毒衣壳有两种主要多肽。从寿光（ＨＨＮＢＶ—９３７）和青岛（ＨＨＮＢＶ—９３８）分离到的病毒种在ＳＤＳ—ＰＡＧＥ和抗ＨＨＮＢＶ—９３７单抗ＥＬＩＳＡ反应上没有显著差异。ＨＨＮＢＶ与斑节对虾杆状病毒（ＭＢＶ）在抗原决定簇上存在差别。  相似文献