引入科研前沿对植物生理学教学方法的改进和效果分析     

龚春梅  王俊惠 《安徽农业科学》2015,(2):373-376

大学生普遍存在科研创新意识差、缺乏科研热情的现象.补充相关科研进展,将科研前沿引进课堂成为一种趋势,笔者对植物生理学光合作用这一章关于补充光合碳同化科研进展与否的教学实践结果进行对比分析,采用调查问卷的形式,围绕已讲授和未讲授光合碳同化适应性进化这一科研前沿进行调查,旨在实现学生对光合碳同化途径多元化发展进化的理解和深化,启发学生对固有知识的质疑和求解热情,达到提升科研兴趣和创新能力,实现精品课程培养满足国家和地方发展需要的高素质人才的目的.  相似文献   

Limited effects of land use on soil dissolved organic matter chemistry as assessed by excitation–emission fluorescence spectroscopy and molecular weight fractionation          下载免费PDF全文

P. Roberts  J. M. Gibbons  P. W. Hill  D. L. Jones  M. Farrell 《Soil Use and Management》2016,32(4):662-665

Dissolved organic matter (DOM) in soil solution represents a complex mixture of organic molecules and plays a central role in carbon and nitrogen cycling in plant–microbial–soil systems. We tested whether excitation–emission matrix (EEM) fluorescence spectroscopy can be used to characterize DOM and support previous findings that the majority of DOM is of high molecular weight (MW). EEM fluorescence spectroscopy was used in conjunction with MW fractionation to characterize DOM in soil solution from a grassland soil land management gradient in North Wales, UK. Data analysis suggested that three distinct fluorescence components could be separated and identified from the EEM data. These components were identified as being of humic‐like or fulvic‐like origin. Contrary to expectations, the majority of the fluorescence signal occurred in the small MW (<1 kDa) fraction, although differences between soils from the differently managed grasslands were more apparent in larger MW fractions. We conclude that following further characterization of the chemical composition of the fluorophores, EEM has potential as a sensitive technique for characterizing the small MW phenolic fraction of DOM in soils.  相似文献   

对我国LULUCF碳计量监测方法的探讨     

高显连 《林业资源管理》2015,(2):46-50

围绕我国林业编制温室气体清单这一重要工作,介绍了IPCC关于LULUCF温室气体清单编制系列技术指南的技术要点和要求,阐明了我国林业温室气体清单编制工作的进展,提出了林业开展LULUCF碳监测工作未来面临的需求。同时,在分析美国、欧盟、加拿大等发达国家LULUCF温室气体清单编制做法和经验的基础上,提出了"在森林资源清查样地系统上构建"、"新布设LULUCF大样地系统"等2种开展LULUCF碳监测的思路,目的是为推动我国林业LULUCF碳监测达到国际先进水平提供建议。  相似文献   

化肥减氮及有机肥配施对水稻产量及土壤养分含量的影响     

解艳玲  贺萌萌  马玲芳  王娟  张骞  谢静波  陈萍 《现代农业科技》2023,(14)

本文旨在研究等磷、钾量条件下，化肥减氮及有机肥不同用量配施对水稻产量及土壤养分的影响，试验共设6个处理，分别为T1∶80%N+20%有机肥；T2∶80%N+40%有机肥；T3∶70%N+60%有机肥；T4∶70%N+80%有机肥；T5∶60%化肥+100%有机肥；CK：100%N+0，对水稻生育期株高、群体动态、产量、构产因子以及秋季土壤养分含量进行分析，结果表明：1化肥减氮20%时，不会对水稻造成明显减产，且当有机肥配施量为500kg·mu-2时，水稻籽粒产量最高，为775.19kg·mu-2，比CK增产17.20%；2有机肥的施用能提高水稻基本苗，对水稻穗长有显著提高效果，对空秕率影响较大，对枝梗数影响最小；3化肥减氮+有机肥配施可以提高土壤肥力，增加土壤有机质含量，提高土壤碱解氮、速效钾含量。  相似文献   

Evaluating biodegradability of soil organic matter by its thermal stability and chemical composition     

《Soil biology & biochemistry》2015

The stability of soil organic matter (SOM) as it relates to resistance to microbial degradation has important implications for nutrient cycling, emission of greenhouse gases, and C sequestration. Hence, there is interest in developing new ways to quantify and characterise the labile and stable forms of SOM. Our objective in this study was to evaluate SOM under widely contrasting management regimes to determine whether the variation in chemical composition and resistance to pyrolysis observed for various constituent C fractions could be related to their resistance to decomposition. Samples from the same soil under permanent pasture, an arable cropping rotation, and chemical fallow were physically fractionated (sand: 2000-50 μm; silt: 50-5 μm, and clay: <5 μm). Biodegradability of the SOM in size fractions and whole soils was assessed in a laboratory mineralization study. Thermal stability was determined by analytical pyrolysis using a Rock-Eval pyrolyser, and chemical composition was characterized by X-ray absorption near-edge structure (XANES) spectroscopy at the C and N K-edges. Relative to the pasture soil, SOM in the arable and fallow soils declined by 30% and 40%, respectively. The mineralization bioassay showed that SOM in whole soil and soil fractions under fallow was less susceptible to biodegradation than that in other management practices. The SOM in the sand fraction was significantly more biodegradable than that in the silt or clay fractions. Analysis by XANES showed a proportional increase in carboxylates and a reduction in amides (protein) and aromatics in the fallow whole soil compared to the pasture and arable soils. Moreover, protein depletion was greatest in the sand fraction of the fallow soil. Sand fractions in fallow and arable soils were, however, relatively enriched in plant-derived phenols, aromatics, and carboxylates compared to the sand fraction of pasture soils. Analytical pyrolysis showed distinct differences in the thermal stability of SOM among the whole soil and their size fractions; it also showed that the loss of SOM generally involved preferential degradation of H-rich compounds. The temperature at which half of the C was pyrolyzed was strongly correlated with mineralizable C, providing good evidence for a link between the biological and thermal stability of SOM.  相似文献   

Environmental stress response limits microbial necromass contributions to soil organic carbon     

《Soil biology & biochemistry》2015

The majority of dead organic material enters the soil carbon pool following initial incorporation into microbial biomass. The decomposition of microbial necromass carbon (C) is, therefore, an important process governing the balance between terrestrial and atmospheric C pools. We tested how abiotic stress (drought), biotic interactions (invertebrate grazing) and physical disturbance influence the biochemistry (C:N ratio and calcium oxalate production) of living fungal cells, and the subsequent stabilization of fungal-derived C after senescence. We traced the fate of ¹³C-labeled necromass from ‘stressed’ and ‘unstressed’ fungi into living soil microbes, dissolved organic carbon (DOC), total soil carbon and respired CO₂. All stressors stimulated the production of calcium oxalate crystals and enhanced the C:N ratios of living fungal mycelia, leading to the formation of ‘recalcitrant’ necromass. Although we were unable to detect consistent effects of stress on the mineralization rates of fungal necromass, a greater proportion of the non-stressed (labile) fungal necromass C was stabilised in soil. Our finding is consistent with the emerging understanding that recalcitrant material is entirely decomposed within soil, but incorporated less efficiently into living microbial biomass and, ultimately, into stable SOC.  相似文献   

44个紫花苜蓿品种的酸铝适应性与耐受性评价     

田政  杨正禹  陆忠杰  罗奔  张茂  董瑞 《草业学报》2023,32(3):142-151

为了解紫花苜蓿在贵州地区的适应性及耐酸铝胁迫机理，以44份紫花苜蓿品种为研究对象，研究紫花苜蓿处于酸铝胁迫下的生理变化，并揭示其生理变化与耐酸铝胁迫间的关系。利用基因与环境互作模型对两个地点1年的紫花苜蓿进行产量分析，筛选出阿尔冈金、新疆大叶苜蓿、Trifecta、Vernal和中牧1号苜蓿5个耐酸铝强适应品种。利用敏感型UC-1465和耐受型阿尔冈金进行酸铝胁迫试验。结果表明：相同处理下，耐受型紫花苜蓿的电导率、相对铝含量、死亡率显著低于敏感型；紫花苜蓿对酸铝胁迫的响应主要通过柠檬酸、苹果酸、乙酸、酒石酸、反丁烯二酸和草酸的显著（P<0.05）增加来体现，其中苹果酸的合成和分泌增多可能是其耐酸铝胁迫的重要原因。  相似文献   

Short‐term releases of CO2 from newly mixed biochar and calcareous soil          下载免费PDF全文

R. Cardelli  M. Becagli  F. Marchini  A. Saviozzi 《Soil Use and Management》2016,32(4):543-545

The work aimed to quantify native organic C mobilized in one calcareous soil in the 21 days after addition of biochar at a range of large to very large applications. The experiment was carried out in unplanted microcosms, and CO₂ flux was used as a measure of net mineralization. A rapid methodological approach, which does not require ¹³C as a tracer, was used to assess any priming effects induced by the biochar. The amount of CO₂‐C mobilized was small relative to the amount of biochar C and proportional to the amount of the biochar added. The additional CO₂‐C was similar to the content of the water‐soluble organic carbon in the biochar added with each application. No interaction with native soil C, that is priming effect, was observed.  相似文献   

Contribution of Different Organs to Grain Filling in Durum Wheat under Mediterranean Conditions I. Contribution of Post‐Anthesis Photosynthesis and Remobilization          下载免费PDF全文

O. Merah  P. Monneveux 《Journal of Agronomy and Crop Science》2015,201(5):344-352

Under Mediterranean conditions, drought affects cereals production principally through a limitation of grain filling. In this study, the respective role of post‐anthesis photosynthesis and carbon remobilization and the contribution of flag leaf, stem, chaff and awns to grain filling were evaluated under Mediterranean conditions in durum wheat (Triticum turgidum var. durum) cultivars. For the purpose, we examined the effects of shading and excision of different parts of the plant and compared carbon isotope discrimination (Δ) in dry matter of flag leaf, stem, chaff, awns and grain at maturity and in sap of stem, flag leaf, chaff and awns, this last measurement providing information on photosynthesis during a short period preceding sampling. Source–sink manipulations and isotopic imprints of different organs on final isotope composition of the grain confirmed the high contribution of both carbons assimilated by ears and remobilized from stems to grain filling, and the relatively low contribution of leaves to grain filling. Grain Δ was highly and significantly associated with grain yield across treatments, suggesting the utilization of this trait as an indicator of source–sink manipulations effects on grain yield. Chaff and awns Δ were better correlated with grain Δ than stem and leaf Δ, indicating that chaff were more involved in grain filling than other organs. Moreover, in chaff, sap Δ was highly significantly correlated with dry matter Δ. These results suggest the use of Δ for a rapid and non‐destructive estimation of the variation in the contribution of different organs to grain filling.  相似文献   

Soil organic carbon stock variability in the Northern Gangetic Plains of India: interaction between agro‐ecological characteristics and cropping systems          下载免费PDF全文

V. K. Singh  M. Rani  B. S. Dwivedi  S. K. Singh  V. K. Gupta  K. Majumdar  R. P. Mishra 《Soil Use and Management》2015,31(4):461-473

Soil organic carbon (SOC) content and its spatial distribution in the Northern Gangetic Plain (NGP) Zone of India were determined to establish the cause–effect relationship between agro‐ecological characteristics, prevailing crop management practices and SOC stock. Area Spread Index (ASI) approach was used to collect soil samples from the NGP areas supporting predominant cropping systems. Exponential ordinary kriging was found most suitable geo‐statistical model for developing SOC surface maps of the NGP. Predicted surface maps indicated that 43.7% area of NGP had 0.5–0.6% SOC, while the rest of the area was equally distributed with high (0.61–0.75%) and low (< 0.5%) SOC content levels. Averaged across cropping systems, maximum SOC content was recorded in Bhabar and Tarai Zone (BTZ), followed by Central Plain Zone (CPZ), Mid‐Western Plain Zone (MWPZ), Western Plain Zone (WPZ) and South‐Western Plain Zone (SWPZ) of the NGP. The SOC stock was above the optimum threshold (> 12.5 Mg/ha) in 97.8, 57.6 and 46.4% areas of BTZ, CPZ and MWPZ, respectively. Only 9.8 and 0.4% area of WPZ and SWPZ, respectively, had SOC stock above the threshold value. The variation in SOC stock was attributed largely to carbon addition through recycling of organic sources, cropping systems, tillage intensity, crop or residue cover and land‐use efficiency, nutrient‐use pattern, soil texture and prevailing ecosystem. Adoption of conservation agriculture, balanced use of nutrients, inclusion of legumes in cropping systems and agro‐forestry were suggested for enhancing SOC stock in the region.  相似文献