Annual variations in brain serotonin and related compounds of domesticated rainbow trout (Oncorhynchus mykiss)     

G. Rozas  P. Rey  M. V. Rozados  E. Rebolledo  M. Aldegunde 《Fish physiology and biochemistry》1992,9(5-6):463-471

The levels of tryptophan (Trp), 5-hydroxytryptamine (serotonin, 5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in two brain regions (hypothalamus and medulla oblongata) of rainbow trout (Oncorhynchus mykiss) were measured throughout the year using a sensitive high performance liquid chromatographic method with electrochemical detection. Trp was also quantified in serum, liver and brain regions. Trp concentrations were higher in sera than in tissues (brain and liver) throughout the year. Hypothalamic and medullary Trp offered similar annual patterns showing two peaks, the first on 10 May and the second on 15 June. 5-HT levels were always higher in the hypothalamus than in the medulla and much higher than the 5-HIAA levels in both regions. Moreover hypothalamic and medullar serotonin patterns were very different, in the latter region few variations were observed. In addition, the 5-HIAA/5-HT ratio was different in the brain regions, the hypothalamus showed two peaks (April 8 and June 15) whereas the equivalent was not observed in the medulla. All measured compounds showed significant variations during the year.  相似文献   

猪对色氨酸需要量的研究     

沈俊  肖凯  胡金杰  陈明洪  彭易柱 《饲料博览》2014,(11):16-20

色氨酸是动物体内必需氨基酸之一,动物对色氨酸需要量的研究已成为当前热点。文章介绍了猪体内色氨酸的分解代谢途径以及色氨酸在体内与其他营养成分的相互作用。文章综述了国内外猪对色氨酸需要量的研究结果,并总结出不同生长阶段猪对色氨酸的需要量。  相似文献   

Growth performance responses to increased tryptophan supplementation in growing barrows fed three different very low crude protein corn and soybean meal-based diets fortified with essential amino acids     

Hiroyuki Sato  Makoto Miura  Takeshi Fujieda  Marcin Taciak  Motoi Kikusato  Kan Sato  Masaaki Toyomizu 《Animal Science Journal》2021,92(1):e13605

Seventy-five individually fed barrows averaging 35 kg were used in a series of three experiments for 6-week growth assays (25 barrows each) to determine the effects of l -tryptophan (Trp) supplementation on growth performance and to establish the lower limits of dietary crude protein (CP) levels. Corn and soybean meal (SBM)-based diets containing 9% (Experiment 1), 10% (Experiment 2), and 11% CP (Experiment 3) fortified with deficient essential amino acids (AAs) except Trp were used as basal diets for each experiment. The experimental diets were supplemented with 0.00%, 0.02%, 0.04%, or 0.06% Trp. A 16% CP corn–SBM-based diet was set as a positive control in each experiment, and feed and water were provided ad libitum. Average daily gain and gain-to-feed ratio improved quadratically (P < 0.05) as supplemented Trp increased in the 9% and 10% CP group, although these positive effects were not observed in the 11% CP group. Because the maximum performance parameters in 9%, 10%, and 11% CP groups were not different from that of the 16% CP positive control group, the marginally reduced level of dietary CP without growth performance being affected appears to be around 9% at most. A potential reduction of nitrogen intake was clearly indicated.  相似文献   

微波水解—氨基酸自动分析仪法测定饲料中色氨酸的含量     

宋洁  王丽芳  姚一萍  史培  钟华晨  郭晨阳  张欣昕  黄洁 《畜牧与饲料科学》2021,42(2):19-25

[目的] 建立微波水解—氨基酸自动分析仪快速测定饲料中色氨酸含量的方法。[方法] 以奶牛全混合日粮饲料（TMR）为研究对象,通过单因素试验和正交试验筛选微波碱水解最佳条件,联合氨基酸自动分析仪LCAK06/Na（4.6 mm×150 mm）色谱柱分离及快速测定饲料中色氨酸含量。[结果] 水解温度是影响饲料色氨酸含量的最主要因素。微波碱水解测定饲料中色氨酸含量的最佳条件是：水解温度170 ℃、水解时间30 min、碱浓度5 mol/L,通过氨基酸自动分析仪测定TMR饲料色氨酸含量为0.14%。色氨酸在0.025~0.600 μmol/mL峰面积与浓度线性关系良好,相关系数（r）为0.999 7。定量限（LOQ）为0.05%,色氨酸回收率93%~104%,精密度RSD为3.58%。[结论] 该方法高效、准确,检测结果与国家标准《饲料中色氨酸的测定》（GB/T 15400—2018）中的常规碱水解法对比无显著差异,对于饲料中色氨酸含量测定具有参考价值。  相似文献   

饲粮色氨酸添加水平对育成期白水貂生长性能、氮代谢及氨基酸消化率的影响     

张雪蕾  崔虎  王静  李光玉  王峰  张铁涛 《动物营养学报》2017,29(11)

本试验旨在研究饲粮色氨酸添加水平对育成期白水貂生长性能、氮代谢及氨基酸消化率的影响。选取60只健康、体重[(0.83±0.09)kg]相近的(60±5)日龄雄性白水貂,随机分成6个组,每组10个重复,每个重复1只水貂。负对照组(Ⅰ组)饲喂粗蛋白质水平为34%的基础饲粮(色氨酸水平为0.22%),试验组分别饲喂在负对照组基础饲粮中添加0.1%(Ⅱ组)、0.3%(Ⅲ组)、0.5%(Ⅳ组)和0.7%色氨酸(Ⅴ组)的试验饲粮,正对照组(Ⅵ组)饲喂粗蛋白质水平为36%的基础饲粮(色氨酸水平为0.22%)。预试期7 d,正试期60 d。结果表明:1)各组水貂60~120日龄平均日增重和平均日采食量差异不显著(P0.05),Ⅲ组、Ⅳ组、Ⅵ组平均日增重略高于其他各组。2)Ⅰ组干物质消化率显著高于Ⅱ组、Ⅲ组、Ⅳ组、Ⅵ组(P0.05)。Ⅴ组、Ⅵ组粗脂肪消化率显著高于Ⅳ组(P0.05)。Ⅳ组、Ⅴ组、Ⅵ组尿氮显著高于Ⅲ组(P0.05)。Ⅰ组、Ⅲ组氮沉积显著高于Ⅳ组(P0.05)。Ⅲ组净蛋白质利用率和蛋白质生物学价值显著高于Ⅳ组、Ⅴ组、Ⅵ组(P0.05)。3)Ⅰ组谷氨酸、苯丙氨酸、赖氨酸、脯氨酸消化率显著高于Ⅲ组(P0.05)。Ⅰ组、Ⅵ组异亮氨酸消化率显著高于Ⅲ组(P0.05)。Ⅰ组、Ⅵ组亮氨酸消化率显著高于Ⅲ组(P0.05)。Ⅴ组丙氨酸消化率显著高于Ⅲ组(P0.05)。Ⅵ组蛋氨酸消化率显著高于其他各组(P0.05),Ⅰ组、Ⅳ组、Ⅴ组蛋氨酸消化率显著高于Ⅱ组、Ⅲ组(P0.05)。Ⅰ组、Ⅴ组、Ⅵ组酪氨酸消化率显著高于Ⅲ组、Ⅳ组(P0.05)。Ⅰ组、Ⅴ组、Ⅵ组半胱氨酸消化率显著高于Ⅳ组(P0.05)。Ⅴ组组氨酸消化率显著高于Ⅱ组、Ⅲ组(P0.05)。Ⅰ组、Ⅲ组、Ⅵ组色氨酸消化率显著高于Ⅱ组(P0.05)。Ⅰ组、Ⅴ组、Ⅵ组精氨酸消化率显著高于Ⅱ组、Ⅲ组(P0.05)。Ⅰ组总氨基酸消化率显著高于Ⅲ组(P0.05)。由此得出,综合生长性能、氮代谢及氨基酸消化率指标,饲粮粗蛋白质水平为34%时,水貂饲粮色氨酸适宜添加水平为0.3%(饲粮色氨酸水平为0.52%)。  相似文献