传染性牛鼻气管炎亚单位疫苗免疫原性的研究     

刘发志  陈毓川  赵青松  刘方玉 《中国预防兽医学报》2001,23(3):206-208

用犊牛睾丸细胞培养传染性牛鼻气管炎病毒（IBRV），反复冻融，差速离心提取病毒，用Triton X-100溶解，超声波处理，制成IBRV TritonX-100亚单位抗原，经SDS－PAGE电泳，其分子量在176kD-53kD之间，其中有6条清晰的蛋白带。该抗原与一定比例的白油－司盘佐剂乳化，接种育成年，经间接ELISA检测，可使接种的育成牛产生高效价的血清抗体（OD492mm=1.57)。2种不同剂量（80mg/头，40mg／头）的IBRV亚单位疫苗接种育成牛，体内抗体效价相差不显著，抗体可在体内持续12周，用IBRV TrionX-100亚单位疫苗2次接种育成牛，其体内抗体效价显著高于用灭活疫苗2次接种育成牛体内的效价。试验结果表明：提取的IBRV多肽制作的亚单位疫苗具有良好的免疫原性，且抗体持续时间较长。  相似文献   

间接持有体制下证券投资者的财产权性质论     

鲁世平  李希平 《湖南农业大学学报(自然科学版)》2016,(4):141-144

20世纪末以来，经济和科技两方面的发展使证券交易体制发生了从直接持有体制到间接持有体制的革命性转换。在证券的间接持有体制下，证券投资者与中介机构之间的法律关系是行纪关系；证券投资者对证券享有的权属的性质是“所有权”。由于同一托管人为众多的证券投资者托管，并且证券投资者对证券享有的所有权是通过支配不包含有体物的财产权行使的，所以证券投资者对证券享有的是一种“按份共有”形式的“准所有权”。  相似文献   

枣树间接地下滴灌根区土壤盐分运移规律研究   总被引：7，自引：0，他引：7  

孙三民  安巧霞  蔡焕杰  姚宝林  乔英 《农业机械学报》2015,46(1):160-169

通过小区试验研究了间接地下滴灌条件下密植枣树根区不同方向(水平及垂直方向)、不同土层盐分质量比的变化特征,及导水装置直径(50、75、90 mm)和灌水量(9、13、17 L/(棵·次))对枣树根区土壤盐分运移的影响。结果表明:经过一段时间后,垂直方向10~40 cm土层盐分质量比较低,0~10 cm土层盐分质量比最高;水平方向10~50 cm各土层盐分质量比随与出水口水平距离的增加而增大。在单次灌水后导水装置直径一定时各土层脱盐率随灌水量的增加而增大。当灌水量一定时,10~40 cm土层脱盐率随导水装置直径的增加呈减小的趋势,0~10 cm、40~50 cm脱盐率随导水装置直径增大呈低-高-低的趋势。在7—9月份,当导水装置直径为75 mm,灌水量为13、17 L/(棵·次)时,0~50 cm土层土壤平均盐分质量比均较低而且变化幅度较小。综合考虑节水、抑盐效果,确定导水装置直径为75 mm,灌水量为13 L/(棵·次)为适宜的间接地下滴灌灌溉模式。  相似文献   

山羊感染伊氏锥虫诊断方法的研究     

廖圣法  汪志楷  施宝坤 《安徽农业大学学报》1988,(2)

本研究结果表明,山羊感染伊氏锥虫(Trypanosoma evansi)后,用血液压滴标本法查虫无效:小白鼠接种法在感染的检查上具有一定的价值;而间接血凝试验(IHA),因其检查结果具有早期性、重复性和可靠性,故被认为在疾病的诊断和带虫的普查上是较为理想的方法。笔者根据183头份血样的IHA测定結果,发现山羊感染锥虫6天以后,血凝效价最低为1:10,最高为1:1280;未感染锥虫的山羊血凝效价始终小于或等于1:10。因此可将1:20及其以上的血凝滴度定为感染的阳性标准,1:10为可疑,小于1:10则属阴性。  相似文献   

LH抗体间接ELISA检测方法的建立   总被引：4，自引：2，他引：4  

崔贞亮  李红飞  吴庆侠  靳亚平  刘璇 《西北农业学报》2007,16(3):26-28

以高纯度的促黄体素（LH）作为包被抗原,建立了检测LH抗体的间接ELISA法,该法的最适抗原包被浓度为1μg/mL,最佳酶标山羊抗小鼠抗体稀释度为1∶1500.该法只与LH阳性小鼠血清呈现阳性反应,而与LH阴性小鼠血清和小鼠促卵泡素（FSH）阳性血清呈现阴性反应.结果表明,该法具有良好的特异性和重复性,可用于LH免疫后抗体水平检测.  相似文献   

豫西脂尾羊体尺与体重的相关性分析   总被引：1，自引：0，他引：1  

田亚磊  高腾云  陈碾管  刘贤  孙宇  付彤  李改英 《江西农业学报》2009,21(5)

运用SPSS软件对139只豫西脂尾羊体尺、体重的测定结果进行相关分析和通径分析,并建立最优回归方程。结果表明:豫西脂尾羊的体重(-Y)与体高(X1)、体长(X2)、胸围(X3)、胸宽(X4)、胸深(X5)、尾宽(X6)、尾长(X7)均呈极显著正相关。胸深、体高、胸宽的直接作用和间接作用对体重的影响都极大。得到最优回归方程为-Y=0.438X1+(-0.209)X2+0.554X5;经t检验,方程具有极显著的真实性和拟合度。  相似文献   

抗猪瘟免疫球蛋白在猪初乳中变化规律研究     

张媛 《吉林畜牧兽医》2007,28(11):7-9

以猪瘟弱毒疫苗免疫空怀母猪,待母猪分娩后,分别收集10d内的乳汁,以建立的间接ELISA法检测猪瘟IgA、IgG、IgM水平,并观察各种抗体动态变化规律。试验结果表明,猪初乳中的IgA、IgG、IgM抗体效价均在分娩当天达到最高值,随后迅速下降,7d后抗体水平与常乳基本一致(>0.05)。对照组母猪仅在分娩当天检测到较低的抗体水平。  相似文献   

应用间接荧光抗体技术快速检测花鲈病原菌——鳗弧菌   总被引：7，自引：2，他引：7  

余俊红  姚斐  俞勇  纪伟尚  徐怀恕 《海洋水产研究》2002,23(2):38-44

以花鲈（Lateolabrax japonicus)弧菌病的病原菌-鳗弧菌（Vibrio anguillarum)W-1为抗原，制备兔抗血清；利用异硫氰酸荧光素标记的羊抗兔免疫球蛋白（FITC-IgG）为荧光标记二抗，并以罗丹明标记的牛血清白蛋白为背景染色，建立检测鳗弧菌的间接荧光抗体快速检测技术。应用该技术对人工感染后的花鲈组织（肌肉、鳃、肠、肾）样品和养殖水体样品进行了鳗弧菌检测，结果显示间接荧光抗体技术不仅可以用于诊断发病的感染花鲈，也可用于检测带菌状态或未发病的感染花鲈。  相似文献   

Seroprevalence of chlamydial infection in dairy cattle in Guangzhou, southern China     

Dong-Hui Zhou  Fu-Rong Zhao  Hui-Yan Xia  Min-Jun Xu  Si-Yang Huang  Hui-Qun Song  Xing-Quan Zhu 《Irish veterinary journal》2013,66(1):1-4

Chlamydia spp. are obligate intracellular gram-negative bacteria that cause a wide range of significant diseases in humans and animals worldwide, resulting in significant economic losses. Chlamydial infection in cattle has been reported in many countries including China. However, there has been no survey of chlamydial infection of dairy cattle in Guangzhou, southern China. The objective of the present investigation was to examine the chlamydial seroprevalence in dairy cattle in Guangzhou, subtropical southern China by using an indirect hemagglutination assay (IHA). The overall seroprevalence of chlamydial infection in dairy cattle was 7.25% (29/400). Greater than or equal to eight-yr-old dairy cattle had the highest seroprevalence (10.34%), followed by those that were ≥ 6 years old or < 7 years old dairy cattle (10.20%), although there were no statistically significant differences among different groups (P > 0.05). Dairy cattle with 5 pregnancies had the highest seroprevalence (10.81%). These results indicate that chlamydial infection was present in dairy cattle in Guangzhou, subtropical southern China, and integrated strategies and measures should be executed to control and prevent chlamydial infection and disease outbreak in the study region.  相似文献   

Prevalence of bovine herpesvirus-4 infection in cats in Central Michigan     

Kruger JM  Venta PJ  Swenson CL  Syring R  Gibbons-Burgener SN  Richter M  Maes RK 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2000,14(6):593-597

The gammaherpesvirus bovine herpesvirus-4 (BHV-4) has been isolated from a wide variety of animals, including lions and domestic cats. Although BHV-4 antibodies have been detected in normal cats and cats with urinary disorders, the epidemiology and pathogenic role of BHV-4 in cats is unknown. The purpose of this study was to determine the prevalence of BHV-4 antibodies and viral nucleic acid in a population of free-roaming cats. Plasma and peripheral blood leukocyte samples were collected from 52 male and 52 female free-roaming cats impounded at a regional animal control facility in Central Michigan. Plasma concentrations of BHV-4 antibodies were measured with an indirect fluorescent antibody test. Peripheral blood leukocyte DNA was isolated, and a 2-stage polymerase chain reaction with heminested primers delineating a conserved portion of the BHV-4 glycoprotein B gene homologue was used to amplify BHV-4-specific DNA sequences. BHV-4 antibodies were detected in 38 (73%) male and 23 (44%) female cats. Seropositive cats were significantly more likely to be male than female (odds ratio = 3.22; P = .007). Cell-associated viremia was detected in 17 (33%) male and 11 (21%) female cats. Of the 61 seropositive cats, 23 (38%) had a detectable viremia; only 5 (12%) seronegative cats had detectable viremia. Seropositive cats were significantly more likely to be viremic than seronegative cats (OR = 4.30: P = .009). Our results suggest that BHV-4 infection may be more widespread in certain cat populations than previously reported. Furthermore, many cats seropositive for BHV-4 antibodies have a concurrent cell-associated viremia.  相似文献