不同牛血清培养BHK—21细胞系的试验     

胡世华  陈应理 《中国兽医科技》1990,(7):25-28

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禽流感新城疫可目视化诊断蛋白芯片的制备及初步应用     

《中国畜禽种业》2009,(6):153-153

超速离心法和蔗糖密度梯度法分别纯化了2种病毒蛋白抗原．用点样缓冲液稀释后点于荃基修饰的片基上，制备蛋白芯片：将芯片与待检血清杂交后，再与胶体金标记的二抗杂交．银染显色后根据灰度值判定结果，从而建立了2种禽病的可视化蛋白芯片鉴别诊断方法。采用该方法对18份现地血清样品进行初步检测．结果显示该芯片可特异性的与相应的抗体杂交，无交叉反应，而且呈现较强的杂交信号，  相似文献   

马铃薯动态生理指标定点差异的比较     

严红  张宏 《马铃薯杂志》1997,(1):27-28

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黄褐天幕毛虫危害指标研究初报   总被引：1，自引：0，他引：1  

于国辉  王志林 《吉林林业科技》1997,(5):29-30

黄褐天幕毛虫在东北地区一年一代，分龄期记录幼虫食叶面积，根据杨树人工林的受害阈限为失叶40％，计算出每平方米面积食叶40％的老龄幼虫数，17条／m2为危害指标。  相似文献   

益生素对肉鸡生产性能和一些生理生化指标影响的研究   总被引：14，自引：0，他引：14  

傅义刚  郑诚 《饲料工业》1997,18(5):39-40

益生素对肉鸡生产性能和一些生理生化指标影响的研究华南农业大学傅义刚郑诚１材料与方法１．１试验用益生素采用广州景泰生物工程有限公司生产的强力益生素，其菌种是从国外引进的典型益生素菌株芽孢杆菌和乳酸菌。实测产品活菌总数为８×１０８ＣＦＵ／ｇ。１．２饲养试...  相似文献   

麻醉剂MS-222对鳙、草鱼效果的初步研究     

夏云麟 《淡水渔业》1985,(2):11-12,4

<正> MS—222(间氨基苯甲酸乙酯甲烷磺酸盐)国外早在五十年代就用于鲑鳟鱼类的短途麻醉运输并取得较好的成效。七十年代进而研究该药物在鱼体内的残留情况及鱼类血液生化指标的变化。但对于此种药物的适宜浓度与全剂量目前尚无一致意见。我国的淡水鱼类—鳙鱼及草鱼,经过MS  相似文献   

梨树盛果期的修剪技术     

谷丽敏 《河北农业科技》2007,(7):31-31

<正>1维持中庸健壮中庸健壮的树势是树体营养生长与生殖生长协调的表现,是保证稳产、高产、优质的前提。中庸健壮树体的树相指标为:树冠外围新梢生长量30厘米左右,且冠内中枝短壮;全树花芽量为全树总枝量的30%左右,且饱满;枝组年青,中小枝组占90%。  相似文献   

油菜高产形态指标与栽培技术     

周克定  周萍 《四川农业科技》1998,(4):11-12

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禽流感、新城疫可目视化诊断蛋白芯片的制备及初步应用     

《今日畜牧兽医》2009,(5)

超速离心法和蔗糖密度梯度法分别纯化了2种病毒蛋白抗原，用点样缓冲液稀释后点于醛基修饰的片基上．制备蛋白芯片；将芯片与待检血清杂交后，再与胶体金标记的二抗杂交，银染显色后根据灰度值判定结果，从而建立了2种禽病的可视化蛋白芯片鉴别诊断方法。采用该方法对18份现地血清样品进行初步检测，结果显示该芯片可特异性的与相应的抗体杂交，无交叉反应，而且呈现较强的杂交信号，其信号灰度值在阳性血清浓度为50－300微克／毫升范围内成线性关系（Y=0．426X＋4．225）。  相似文献   

Locomotion Score and Its Impacts on Production Performance and Blood Routine Indexes in Chinese Holstein     

GAO Qing  SHI Jizhen  YANG Zhichao  LUO Sunlin  ZHANG Siwei  HUANG Shangzhen  ZHANG Hailiang  LI Junwei  GUO Gang  WANG Yachun 《中国畜牧兽医》2007,47(12):4016-4023

To investigate the influencing factors of locomotion score (LS) and its impacts on production performance and blood routine index in Holstein,body condition score (BCS),LS and DHI (dairy herd improvement) of 1 486 lactating cows from two dairy farms,and the blood routine indexes of 101 lactating cows from one farm in Beijing were measured.The fixed model was used to analyze the impacts of herd,parity and lactation stage on LS,and the impacts of LS and BCS on daily milk yield,somatic cell score,milk composition and blood routine indexes in dairy cows.The results showed that both parity and lactation stage had significant impacts on LS.The LS increased with the increase of parit,the LS increased by 0.71 from first lactation to fifth and above lactation.The LS of cow in lactation stage Ⅲ(100-199 d) and Ⅵ (after 350 d) were significantly higher than cow in stage Ⅱ (45-99 d)(P<0.05),and both were higher than 2.The LS had no significant impacts on daily milk yield,milk composition and somatic cell score (P>0.05).However,the milk yield of the cow with LS≥4 was the lowest,and a decrease of 2.29 kg was found compared with the cow with LS=1.Among the various blood routine indexes,LS had significant impacts on red blood cell count,hemoglobin content,mean corpuscular hemoglobin concentration and lymphocyte count (P<0.05),and had no significant impacts on white blood cell count (P>0.05).With the increase of LS,the red blood cell count,hemoglobin content,mean corpuscular hemoglobin concentration and lymphocyte count of cows decreased with different extents.There were some problems on the body immunity and hematopoiesis of cows with poor LS.In dairy farm,regular evaluation of LS could be used to find the management problems of feet and legs,which had a great significance to reduce the loss of production performance and early culling caused by hoof disease.  相似文献