普通荞麦染色体的原位PCR技术研究     

李分龙  陈庆富 《安徽农业科学》2011,(17):10135-10138

[目的]探索一种适合荞麦的简单易行的染色体原位PCR技术。[方法]采用16S套式引物、4．5S套式引物与psbA引物，以栽培甜荞为材料，进行染色体原位PCR、原位套式PCR与多次原位PCR试验。[结果]高温干燥可以起到与包埋类似的作用；染色体的原位套式PCR效果比原位PCR明显，多次原位PCR次数为5-6效果较佳。16S引物和4．5S引物均显示了4对信号，但位置不同；而psbA引物是单拷贝的，仅显示出1对信号。根据这些信号的位置差异可以区分普通荞麦的5对染色体。[结论]所使用的荞麦染色体原位PCR技术简单易行。  相似文献   

鲍曼不动杆菌雏鸡分离株CCGGD201101的分离、鉴定及致病性研究简     

邹德颖  刘东  郭兴  陈晓峰  胡盼  卢士英  周玉  柳增善  任洪林  于师宇 《中国畜牧兽医》2014,41(1):178-181

试验旨在鉴定吉林省某雏鸡孵育基地病死雏鸡组织中分离出的1株致病性菌CCGGD201101株并测定其致病性。对疑似致病菌进行生理生化试验、16S rDNA测序鉴定，并人工接种昆明鼠，测定其半数致死量，验证细菌毒力。经鉴定该菌为鲍曼不动杆菌（Acinetobacter baumannii）。以鲍曼不动杆菌CCGGD201101分离株为研究对象，并以鲍曼不动杆菌标准株（ATCC 19606）为对照，测得半数致死量，进一步证明鲍曼不动杆菌病死鸡分离株CCGGD201101具有较强致病性。  相似文献   

基于RFID技术的电动车充电系统     

《科技视界》2016,(3)

本文设计了基于RFID技术的一种刷卡式的充电服务终端硬件装置,实现刷卡自动计费。本程序以AT89S52单片机为主控制器,用MFRC500射频识别芯片进行识别,并与射频卡进行通信。  相似文献   

Sequence and Phylogenetic Analysis of rDNA ITS of Three Eimeria Species in Rabbit     

XU Jia-yuan  LIAO Gui-cheng  HU Hui-peng  CHEN Xiao-zhen  LIU Li-dan  WU Song-ming  WEN Yuan-hui  LIU Yuan  WENG Ya-biao  LIN Rui-qing 《中国畜牧兽医》2014,41(12):30-33

In order to study whether the internal transcribed spacers (ITS) sequence could be used as a molecular marker for the species identification of rabbit coccidian, the rDNA ITS of Eimeria intestinalis, Eimeria flavescens and Eimeria magna were amplified by polymerase chain reaction (PCR), and were cloned into pGEM-T Easy vector subsequently. The positive recombinant plasmids were identified by PCR and then sequenced. By sequence comparison and comparative analysis with the relative sequences of rabbit Eimeria spp. available in GenBank, the results showed that the lengths of Eimeria intestinalis, Eimeria flavescens and Eimeria magna were 1065, 1009 and 1047 bp, respectively, and the sequence homologies with the same species sequences were 99.2%, 99.0% and 94.5%, respectively, while were 55.3% to 82.1% compared with corresponding sequences of other different species sequences. The phylogenetic analysis using software Mega 5.0 showed that all rabbit coccidia clustered together in a clade, which was divided into two sister lineages, corresponding to the presence or absence of oocyst residuum. The result demonstrated ITS could be used as a molecular marker for the species identification of rabbit coccidia.  相似文献   

秸秆纤维素分解细菌的筛选与鉴定     

洪权春  李红雨  刘秀花 《湖北农业科学》2016,(13):3327-3330

利用纤维素琼脂选择培养基和刚果红染色方法,从不同来源的土样中分离出2个菌株,测定了其纤维素酶活性、小麦秸秆降解能力,并对纤维素降解能力强的两个菌株进行了分子鉴定。结果表明,两株高效纤维素分解菌在纤维素刚果红培养基上均能形成透明水解圈,利用16S r RNA序列测序并分析Genbank中的同源性,结合形态和理化性质确定为Bacillus thuringiensis和Bacillus anthracis。在37℃,p H为7的条件下测定了纤维素酶活力,SC1-3和XR2-5-4分别为1.41和1.59。这两株37℃下经15 d后小麦秸秆失重率达19.92%和22.83%。  相似文献   

Devastation of sesame (Sesamum indicum L.) crops in different agroclimatic zones of India by genetically diverse subgroups of phytoplasma     

《Crop Protection》2016

The sesame crop is highly susceptible to infection by phytoplasmas, a class of cell wall-less plant pathogenic bacteria (Mollicutes), which is responsible for widespread loss of sesame crops in both North and South India in recent years. Therefore, characterizing the pathogen population is required before the control measures can be devised and implemented. With molecular tools based on nested polymerase chain reaction (PCR) assays, sequencing, restriction profiling, and phylogenetic analysis, phyllody-affected sesame plants collected from nine different states of India were found to be infected by phytoplasmas belonging to two 16Sr groups, namely 16SrI and II. Two subgroups of phytoplasma −16SrI-B and 16SrII-D— were prevalent in symptomatic sesame samples collected from North India, whereas phytoplasma of only the 16SrII group was found in South India. However, the latter samples were diverse, belonging to three different subgroups (16SrII-A, II-C, and II-D). In addition, yearly phyllody-affected sesame samples from Delhi for 4 consecutive years (2007–2010) showed variation in the infecting phytoplasma: the subgroup 16SrII-D was detected in samples collected in 2007, and 16SrI-B was predominantly found in the samples collected in the subsequent years. The study also provides molecular evidence for the association between 16SrI-B phytoplasma and different symptoms in sesame crops such as fasciation, little leaf, and stunting. This is the first study to report the association of the phytoplasma subgroups 16SrII-A and II-D with sesame crops in India. This study provides a baseline for designing specific detection and molecular analysis strategies for quarantine purposes. It also highlights the need for examining the dynamics of seasonal or location-specific variation in vector populations to determine the pattern of infection outbreaks.  相似文献   

引入6S管理体系提高个体食品生产者职业素质     

樊军浩  李红利  孟宏昌  李东亚 《农产品加工．学刊》2011,(9):111-113,128

介绍了6S管理的内涵、实施方案,以及引入6S管理的必要性与可行性。针对当地个体食品生产者生产馒头的现状,引入6S管理体系,取得了良好的效果。  相似文献   

高校实验室信息管理系统的设计与实现     

寇宏达  靳志强 《河北农业大学学报》2013,(1):113-117

基于对高校实验室科学化、网络化管理的目的,采用查阅大量相关文献资料,分析国内外研究现状,进行系统设计、实现,并通过实践验证研究效果的技术路线,构建了基于B/S结构模式的高校实验室信息管理系统。通过在河北农业大学基础化学实验室的具体应用,达到了实验室无纸化办公、高效率管理的效果。  相似文献   

战后美国经济周期再考察     

刘军荣 《湖南农业大学学报(自然科学版)》2013,(5):120-124

二战后美国经济周期波动发生显著变化。相比二战之前，美国经济周期扩张期拉长，衰退期缩短，二者之间形成剪刀差。经济周期长度、扩张期跨度和紧缩期跨度的离散度远远大于战前。二战后至今，美国经济中周期出现（朱格拉周期）呈拉长的趋势，扩张期远远长于衰退期，中周期（朱格拉周期）和短周期（基钦周期）的波幅经历了从小到大的阶段性变化。  相似文献   

Bacterial community analysis of purulent material from liver abscesses of crossbred cattle and Holstein steers fed finishing diets with or without tylosin     

Raghavendra G Amachawadi  Wesley A Tom  Michael P Hays  Samodha C Fernando  Philip R Hardwidge  T G Nagaraja 《Journal of animal science》2021,99(4)

Liver abscesses in feedlot cattle are polymicrobial infections. Culture-based studies have identified Fusobacterium necrophorum as the primary causative agent, but a number of other bacterial species are frequently isolated. The incidence of liver abscesses is highly variable and is affected by a number of factors, including cattle type. Holstein steers raised for beef production have a higher incidence than crossbred feedlot cattle. Tylosin is the commonly used antimicrobial feed additive to reduce the incidence of liver abscesses. The objective of this study was to utilize 16S ribosomal RNA amplicon sequence analyses to analyze the bacterial community composition of purulent material of liver abscesses of crossbred cattle (n = 24) and Holstein steers (n = 24), each fed finishing diet with or without tylosin. DNA was extracted and the V3 and V4 regions of the 16S rRNA gene were amplified, sequenced, and analyzed. The minimum, mean, and maximum sequence reads per sample were 996, 177,070, and 877,770, respectively, across all the liver abscess samples. Sequence analyses identified 5 phyla, 14 families, 98 genera, and 102 amplicon sequence variants (ASV) in the 4 treatment groups. The dominant phyla identified were Fusobacteria (52% of total reads) and Proteobacteria (33%). Of the top 25 genera identified, 17 genera were Gram negative and 8 were Gram positive. The top 3 genera, which accounted for 75% of the total reads, in the order of abundance, were Fusobacterium, Pseudomonas, and Bacteroides. The relative abundance, expressed as percent of total reads, of phyla, family, and genera did not differ (P > 0.05) between the 4 treatment groups. Generic richness and evenness, determined by Shannon–Weiner and Simpson’s diversity indices, respectively, did not differ between the groups. The UniFrac distance matrices data revealed no clustering of the ASV indicating variance between the samples within each treatment group. Co-occurrence network analysis at the genus level indicated a strong association of Fusobacterium with 15 other genera, and not all of them have been previously isolated from liver abscesses. In conclusion, the culture-independent method identified the bacterial composition of liver abscesses as predominantly Gram negative and Fusobacterium as the dominant genus, followed by Pseudomonas. The bacterial community composition did not differ between crossbred and Holstein steers fed finishing diets with or without tylosin.  相似文献