The Occurrence of a Congenital Malformation in the Sixth and Seventh Cervical Vertebrae Predominantly Observed in Thoroughbred Horses     

《Journal of Equine Veterinary Science》2014,34(11-12):1313-1317

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Serum Biomarkers of Clinical and Cytologic Response in Dogs with Idiopathic Immune‐Mediated Polyarthropathy     

J.D. Foster  S. Sample  R. Kohler  K. Watson  P. Muir  L.A. Trepanier 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2014,28(3):905-911

Background

Immune‐mediated polyarthopathy (IMPA) is common in dogs, and is monitored by serial arthrocenteses.

Hypothesis/Objectives

Plasma C‐reactive protein (CRP), interleukin‐6 (IL‐6), and CXCL8 (interleukin‐8) would serve as noninvasive markers of joint inflammation in IMPA.

Animals

Nine client‐owned dogs with idiopathic IMPA; 6 healthy controls.

Methods

Prospective study. Plasma CRP, IL‐6, and CXCL8 were measured by ELISA at baseline, 2, and 4 weeks during treatment with prednisone at 50 mg/m²/day. Arthrocenteses, the canine brief pain inventory (CBPI), and accelerometry collars were used to assess joint inflammation, lameness, and mobility at all 3 time points.

Results

C‐reactive protein concentrations were higher in IMPA dogs (median 91.1 μg/mL, range 76.7–195.0) compared with controls (median <6.3 μg/mL, <6.3–13.7; P = .0035), and were significantly lower at week 2 (10.6 μg/mL, <6.3–48.8) and week 4 (<6.3 μg/mL, <6.3–24.4; P < .001).C‐reactive protein was correlated with median CBPI scores (r = 0.68; P = .0004), joint cellularity (r = 0.49, P = .011), and mobility by accelerometry (r = −0.42, P = .048). Plasma IL‐6 concentrations were also higher in IMPA dogs (median 45.9 pg/mL), compared with controls (median <15.7 pg/mL; P = .0008). IL‐6 was lower in IMPA dogs by week 4 (<15.7 pg/mL; P = .0099), and was modestly correlated with CBPI scores (r = 0.47, P = .023). CXCL8 did not differ significantly between IMPA and healthy dogs.

Conclusions

Plasma CRP and IL‐6 might be useful surrogate markers of synovial inflammation and disease activity in dogs with IMPA.  相似文献   

Expression of Krüppel-Like Factor 6, KLF6, in Rat Pituitary Stem/Progenitor Cells and Its Regulation of the PRRX2 Gene     

Hiroki UEHARU  Masashi HIGUCHI  Naoto NISHIMURA  Saishu YOSHIDA  Shiori SHIBUYA  Kenta SENSUI  Takako KATO  Yukio KATO 《The Journal of reproduction and development》2014,60(4):304-311

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产蛋循环过程中TRPV6、CaBP-D_（28K）和PMCA 1b在蛋壳腺的动态表达     

翟晓虎  ;贺卫华  ;侯加法  ;杨俊花 《兽医大学学报》2014,(9):1534-1539

将40羽220日龄ISA蛋鸡分为5组,于产蛋后0、2、4.5、8、16h断头处死,采集蛋壳腺组织,运用Real-time PCR和Western-blot方法检测产蛋循环过程中瞬时性受体单位香草精受体6（Transient receptor potential vanilloid receptor 6,TRPV6）、钙结合蛋白（calbindin D28K,CaBP-D28K）、质膜钙离子ATP酶1b（plasma membrane Ca2＋-ATPase 1b,PMCA 1b）mRNA和蛋白浓度的动态变化。结果显示,卵子未进入蛋壳腺前（产蛋后0~4.5h）,蛋壳腺内TRPV6、CaBP-D28K和PMCA 1bmRNA表达水平较低,随后表达量逐渐升高,在蛋壳钙化过程中达到最大值（产蛋后16h）,与0h相比,TRPV6和CaBP-D28KmRNA表达差异均极显著（P〈0.01）;另外,产蛋循环过程中,TRPV6、CaBP-D28K和PMCA 1b蛋白浓度变化与mRNA变化一致,产蛋后16h到达最大值,其中CaBP-D28K蛋白浓度与0h相比,差异显著（P〈0.05）。结果表明,产蛋循环可调控蛋壳腺内TRPV6、CaBP-D28K和PMCA 1b的表达,并提示钙离子跨细胞转运途径在钙离子进入蛋壳腺形成蛋壳过程中发挥重要作用。  相似文献   

水牛RNA聚合酶Ⅲ启动子的克隆与鉴定     

张晓溪  刘庆友  邓彦飞  罗婵  崔奎青  石德顺 《广西农业科学》2014,(5):858-863

[目的]获得有效的水牛RNA聚合酶Ⅲ启动子序列,为开展水牛源细胞的基因特异沉默研究奠定基础.[方法]通过启动子上、下游保守序列对水牛源启动子7SK、U6进行克隆和启动子关键顺式作用元件识别,利用一段针对EGFP的shRNA片段（shEGFP）对水牛7SK、U6启动子进行功能性分析,然后分别在水牛源及鼠源细胞中与pEGFP-N1共转染,转染48h后用荧光显微镜检测EGFP的表达情况,并用流式细胞仪和荧光实时定量PCR检测EGFP沉默表达情况.[结果]克隆获得水牛7SK、U6启动子序列分别为430和357 bp,其OCT-1（或CACCC盒）及TATA盒高度保守.连接shEGFP后与pEGFP-N1共转染细胞,通过荧光显微镜可观察到细胞发生了明显的荧光表达沉默现象;通过流式细胞分析,发现水牛7SK和U6启动子引导的shEGFP在水牛源细胞中沉默效率高达93.82％和87.45％;荧光实时定量PCR检测结果显示,在转染bu7SK-shEGFP和buU6-shEGFP的水牛源BFF细胞中,EGFP表达水平均显著低于其他物种启动子的细胞转染组（P＜0.05）,而在鼠源PT67细胞中,水牛启动子的启动效率与其他物种启动子差异不显著（P＞0.05）.[结论]水牛7SK和U6启动子可高效启动shRNA表达,且具有一定的物种特异性.  相似文献   

四倍体少籽西瓜新品种兴桂六号的选育     

何毅  ;李文信  ;陆杰思  ;李桂芬  ;李智  ;覃斯华  ;黄金艳  ;周思菊  ;洪日新  ;柳唐镜  ;李天艳  ;樊学军 《广西农业科学》2014,(12):2203-2207

【目的】选育适合露地爬地栽培和大棚立架栽培的四倍体西瓜新品种,提高广西西瓜产业竞争力。【方法】于2008年利用四倍体西瓜403作母本,四倍体西瓜JL作父本,配组选育四倍体一代杂交种兴桂六号,2009-2010年,连续两年进行露地爬地栽培品比试验,2011-2012年,连续两年进行大棚立架栽培品比试验。【结果】兴桂六号全生育期春造90-100 d,秋造65-80 d,果实发育期29-35 d。单果重3.5-4.5 kg,果实圆形,表皮深绿覆盖墨绿条带,有光泽,皮厚1.0-1.2 cm,耐贮运。果肉大红色,剖面光滑,纤维黄筋少,种籽少,汁多,质实、细密、清甜爽口,品质优,中心含糖11.5%。2009-2010年,连续两年露地爬地栽培品比试验,平均产量分别为38081.3和34602.8 kg/ha,分别比对照增产15.6%和13.7%。2011-2012年,连续两年大棚立架栽培品比试验平均产量分别为66577.5和63169.5 kg/ha,分别比对照增产18.1%和15.0%。2012年广西西瓜区域试验平均产量34182.0 kg/ha,比对照增产10.8%;2010-2012年,露地爬地生产栽培示范试验平均产量32550.0-36585.0 kg/ha,比对照增产10.6%-15.5%;大棚立架生产栽培试验平均产量60178.5-63168.0 kg/ha,比对照增产12.6%-15.2%。【结论】四倍体杂交西瓜新品种兴桂六号适宜露地栽培和大棚立体栽培,在广西西瓜种植区均可推广种植。  相似文献   

Optimization of triploidy induction in the blacklip abalone, Haliotis rubra (Leach, 1814), using 6-dimethylaminopurine     

Wenshan Liu  Michael Heasman  & Rod Simpson 《Aquaculture Research》2004,35(11):1076-1085

Optimal conditions of 6‐dimethylaminopurine (6‐DMAP) for triploidy induction in the blacklip abalone Haliotis rubra (Leach, 1814) were investigated, targeting inhibition of second polar body (PB2) formation. Two experiments were conducted at a water temperature of 17.5–18.56;C where factorial combination of (1) four dosages (DSs) of 100, 150, 200 and 250 μM 6‐DMAP, four starting times (STs) of 15, 20, 25 and 30 min post fertilization, and two treatment durations (TDs) of 20 and 30 min and (2) three DSs of 50, 100 and 150 μM 6‐DMAP, three STs of 15, 20 and 25 min post fertilization, and three TDs of 10, 20 and 30 min, were applied respectively. Day 3 larvae were sampled for triploidy and survival. Percent triploidy was verified using flow cytometry (FCM). Results show that optimal inductions that combine both high rates of triploidy and reasonable survival were those treatments commenced 15 or 20 min post fertilization and continued for 20 or 30 min, using 100 μM 6‐DMAP. These conditions induced rates of triploidy and relative survival of 80.5–93.3% and 36.5–40.2%, respectively, in the first experiment, and corresponding rates were 79.1–93.6% and 20.7–43.0% in the second experiment. High percent triploidy were also obtained in a number of treatments using 150 μM 6‐DMAP, but with overall survival rates generally lower than those using 100 μM 6‐DMAP.  相似文献   

Effect of crude oil extracts from trout offal as a replacement for fish oil in the diets of the Australian native fish Murray cod Maccullochella peelii peelii     

Giovanni M Turchini  Rasanthi M Gunasekera  & Sena S De Silva 《Aquaculture Research》2003,34(9):697-708

The efficacy of trout oil (TO), extracted from trout offal from the aquaculture industry, was evaluated in juvenile Murray cod Maccullochella peelii peelii (25.4±0.81 g) diets in an experiment conducted over 60 days at 23.7±0.8 6;C. Five isonitrogenous (48% protein), isolipidic (16%) and isoenergetic (21.8 kJ g^?1) diets, in which the fish oil fraction was replaced in increments of 25% (0–100%), were used. The best growth and feed efficiency was observed in fish fed diets containing 50–75% TO. The relationship of specific growth rate (SGR), food conversion ratio (FCR) and protein efficiency ratio (PER) to the amount of TO in the diets was described in each case by second‐order polynomial equations (P<0.05), which were: SGR=–0.44TO²+0.52TO+1.23 (r²=0.90, P<0.05); FCR=0.53TO²–0.64TO+1.21 (r²=0.95, P<0.05); and PER=–0.73TO²+0.90TO+1.54 (r²=0.90, P<0.05). Significant differences in carcass and muscle proximate compositions were noted among the different dietary treatments. Less lipid was found in muscle than in carcass. The fatty acids found in highest amounts in Murray cod, irrespective of the dietary treatment, were palmitic acid (16:0), oleic acid (18:1n‐9), linoleic acid (18:2n‐6) and eicosapentaenoic acid (20:5n‐3). The fatty acid composition of the muscle reflected that of the diets. Both the n‐6 fatty acid content and the n‐3 to n‐6 ratio were significantly (P<0.05) related to growth parameters, the relationships being as follows. Percentage of n‐6 in diet (X) to SGR and FCR: SGR=–0.12X²+3.96X–32.51 (r²=0.96) and FCR=0.13X²–4.47X+39.39 (r²=0.98); and n‐3:n‐6 ratio (Z) to SGR, FCR, PER: SGR=–2.02Z²+5.01Z–1.74 (r²=0.88), FCR=2.31Z²–5.70Z+4.54 (r²=0.93) and PER=–3.12Z²–7.56Z+2.80 (r²=0.88) respectively. It is evident from this study that TO could be used effectively in Murray cod diets, and that an n‐3:n‐6 ratio of 1.2 results in the best growth performance in Murray cod.  相似文献   

Dramatic Changes of Matrix Metalloproteinases-7 and Lysozyme in the Ulcerative Colitis of Mice Induced by Dextran Sulfate Sodium     

KANG Jing-jing  ZHAO De-ming  TENG Ke-dao  JIAO Xi-lan  WANG Ping-li  SUN Zhe  NI Pei-pei  WANG Zhi-feng  ZHANG Rui  YANG Yu-rong  LIANG Hong-de 《农业科学学报》2014,13(4):858-869

Ulcerative colitis （UC） is a lifelong illness with profound emotional and social impacts, and could cause serious damage to large intestine, especially in colon. However, the pathogenesis of UC remained unclear. The present study attempts to find out the role of matrix metalloproteinases-7 （MMP-7） and lysozyme in the pathogenesis of UC through a mice model induced by dextran sulfate sodium （DSS）. The UC model was evaluated both by disease activity index （DAI） and the intestinal histopathology. The results show that there is a high correlation between the DAI score and the pathological changes of colon. Interleukin-6 （IL-6） serum levels and large intestinal fluids levels in UC mice are always higher than that of the control groups, which might be associated with the degree of the inflammation damage in the colon. The change tendency of the MMP-7 mRNA and protein expressions are both up-regulated firstly and then down-regulated from 1 to 5 d in the colon, but only the MMP-7 protein is up-regulated at 7 d again. The up-regulated MMP-7 levels in the early stage of UC may play a protective role through the activated defensins, while the down-regulated levels in the mid-later stage of UC may be connected with the severe lesions in the colon. However, the up-regulated MMP-7 levels in the later stage of UC in the colon may also contribute to the tissue repair or be served as a marker to CRC （colorectal cancer）. The distribution of lysozyme protein indicates that there may be Paneth-like cells in the colon. Both the changes of MMP-7 and lysozyme in the small intestine may play a protective role for the safe environment of the whole gut, especially to the colon of UC.  相似文献   

Isolation of Atypical Genotype Actinobacillus pleuropneumoniae Serotype 6 in Japan     

Hiroya ITO  Kiyohito KATSURAGI  Shunsuke AKAMA  Hirofumi YUZAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(4):601-604

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