RAPD及其在果树上的应用     

李发芳  罗正荣  蔡礼鸿 《果树学报》1998,(3)

就RAPD的特点、操作及其在果树品种鉴定、遗传多样性检测、系谱解析和遗传图谱构建等方面的应用和进展进行了简要的阐述。  相似文献   

乌兰布和荒漠人工绿洲小气候效应研究   总被引：7，自引：0，他引：7  

王君厚  周士威 《干旱区研究》1998,15(1):27-34

通过对乌兰布和荒漠东北边缘人工绿洲内和荒漠对照区各小气候因子连续１２年的同步观察,采用模糊综合评判方法,全面定量分析了防护森体系不同时期的小气候效应及变化规律,评价结果表明,绿洲防护要体系对各气象因子有前显的影响,特别是降低风速,抑制蒸发,提高空气湿度等方面尤为突出,综合小气候效应明显,１２年间效益值增加了０．７４６,增长率为３１％,绿洲防护林体系对小气候的改善作用各时期不同,从综合效益值来看,初  相似文献   

乌鲁木齐河流域土地灌溉系统的灰色决策模型研究     

付炜 《干旱区资源与环境》1998,12(1):75-82

本文以乌鲁木齐河流域为试验区,介绍了干旱区流域土地灌溉系统的多目标灰色决策模型的构造原理和方法。利用灰色控制系统的理论和方法建立了灰色优势分析模型,并以该模型为基础建造了多目标灰色决策分析模型。对最优局势的效果测度与综合决策矩阵的构成原理进行了深入探讨,并由此构造了地下水位控制模型与土地灌溉系统的灰色决策模型,为流域地下水位的控制提供了一种新的研究方法。  相似文献   

乙脑病毒PrME基因在杆状病毒表达系统中的表达   总被引：2，自引：0，他引：2  

刘金华  高福 《畜牧兽医学报》1998,29(6):546-552

本试验将乙型脑炎病毒（ＪＥＶ）的ＰｒＭ／Ｅ基因的ＨｉｎｄⅢ－ＢｇｌⅡ片段（２．１ｋｂ）插入到杆状病毒载体ｐＡｃＵＷ３１的ＢａｍＨＩ位点,使外源基因置于多角体蛋白启动予下游,构建成转移载体ｐＡｃＵＷ３１ＪＥ,以Ｌｉｐｏｆｅｃｔｉｎ作为共转染试剂,将纯化的ｐＡｃＵＷ３１ＪＥ与Ｂｓｕ３６Ｉ线性化的杆状病毒ＡｃＭＮＰＶ．ＬａｃＺＤＮＡ共转染昆虫ｓｆ９经病毒蚀斑纯化技术和Ｘ－ｇａｌ与中性红双重染色技术,随机  相似文献   

用发酵罐制备嗜水气单胞菌疫苗用菌液培养条件的优化   总被引：5，自引：0，他引：5  

范红结  陈怀青  陆承平 《中国兽药杂志》1998,32(2):11-13

本试验探讨了用发酵罐培养嗜水气单胞菌灭活疫苗生产菌株Ｊ－１株菌液的最佳条件。分别以发酵罐和摇床在不同的培养条件下培养Ｊ－１株制备菌液,前者１２个批次,后者６个批次。比较不同的培养条件对细菌菌体数量及ＨＥＣ毒素产量的影响。结果表明：发酵罐培养优于摇床培养,以产毒素培养基于２８℃发酵罐通气培养Ｊ－１株,培养２８小时效果最佳,培养菌液含菌量达２．９×１０１０ｃｆｕ／ｍｌ,培养液上清的溶血价达２７,发酵液中残糖为０．１２ｇ／Ｌ,符合发酵工艺要求。  相似文献   

牧草抗旱性综合评价指标体系的AHP模型设计与应用的研究   总被引：18，自引：1，他引：17  

任Jun 《草业学报》1998,7(3):34-40

在查阅大量牧草抗旱性鉴定指标研究资料的基础上,应用层次分析法（ＡＨＰ模型,ＡｎａｌｙｔｉｃＨｉｅｒ－ａｒｃｈｙＰｒｏｃｅｓｓ）理论,根据专家咨询系统的反馈信息,对所选择的６类抗旱性鉴定指标及其２１个指标变量,进行比较判断矩阵的构建和计算,定量确定了各指标变量在牧草抗旱性综合评价体系中的权重及重要程度次序。  相似文献   

球根鸢尾的病毒鉴定及试管脱毒成球技术   总被引：7，自引：0，他引：7  

袁梅芳 《园艺学报》1998,25(2):175-178

对上海地区球根鸢尾进行了病毒病害调查。经检测初步确定球根鸢尾普遍存在鸢尾重花叶病毒、鸢尾轻花叶病毒、水仙潜隐病毒３种病毒的复合侵染。对带毒种球进行茎尖组培脱毒,得出了茎尖诱导成芽、芽成球及小球生根的培养基配方。将组培苗在试管苗、成球苗、移栽苗等不同时期进行病毒检测,结果表明,小于１．０ｍｍ的茎尖诱导的幼苗可脱去上述３种病毒。  相似文献   

建筑围护结构动态热特性辨识及其系统     

陈友明  张国强  陈在康 《湖南农业大学学报(自然科学版)》1999,26(4)

提出了建筑围护结构动态热特性辨识的概念，阐述了围护结构动态热特性辨识的方法及其作用，介绍了一种新的围护结构动态热特性辨识系统的构成和特点。  相似文献   

规模种植条件下玉米茬地平翻整地机组经济性评价   总被引：1，自引：0，他引：1  

乔金友  张雪珍  王文富  郝雨萱  洪魁  张健 《农机化研究》2017,(10):67-71

玉米后茬耕整地是玉米机械化生产过程中的重要环节之一,评价玉米茬地机械化耕整地机组的经济性能对指导农业及农业机械化生产具有重要意义。根据实际调研结果,确定了规模化生产条件下玉米茬地平翻整地工艺过程及完成各项作业的10种适用机组;选择年最大盈利值、投资回收期和内部收益率评价玉米茬地平翻整地各机组的经济性能,给出了各指标的计算模型。评价结果表明:东方红1304拖拉机灭茬机组、CASE210拖拉机耙地机组年收益最大;东方红1304拖拉机起垄机组、CASE210拖拉机平翻机组可获得较高的投资效率。  相似文献   

Diversity of methodologies to experiment Integrated Pest Management in arable cropping systems: Analysis and reflections based on a European network     

《European Journal of Agronomy》2017

Integrated Pest Management (IPM) aims to promote physical and biological regulation strategies that help farmers contain populations of pests (pathogens, animal pests and weeds) and to finally reduce the reliance on pesticides. It is based on the holistic combination of multiple management measures rather than on the sum of single methods, each of them having only small effects on pests reduction. Thus, to analyse the interactions between IPM measures and to evaluate the sustainability of their implementation, we require an approach considering the whole cropping system (CS), i.e. a functional entity whose complexity is more than the sum of its parts. A network of European experiments at the CS level was set up recently, and aimed at sharing data and expertise to enhance knowledge of IPM. Comparison of existing methodologies highlighted a diversity of CS designs and experimental layouts. We deduced that the concept of CS itself was viewed differently among scientists, and this affected experimental protocols. Other differences were related to the research context and objectives. Some experiments aimed to explore very innovative strategies and generated knowledge on both their effects on the agroecosystem and their ability to satisfy a set of performance targets, while others aimed to provide quickly adoptable solutions for local farmers in line with the current socio-economic constraints. In some research programmes, the experiment was part of the CS design process — and tested CS were regularly revised based on an continuous improvement loop — while in other cases CS were kept stable across years so as to enable the evaluation of their long-term cumulative effects. A critical aspect contributing to the diversity among CS experiments was the distinction between a factorial design of experimental CS and systemic approaches: factorial experiments allowed quantification of the effects of each IPM component regardless of the consistency between components defining the CS. In contrast, systemic approaches focused on the overall evaluation of CS designed with consideration of their consistency, hence maximising their ability to meet the objectives. Because CS experiments represent a huge investment in terms of economics and time, preliminary reflections of the relevance of the experimental strategy is of critical importance.  相似文献