国产杀螨新药双甲脒乳剂防治獭兔螨病试验及大面积推广应用   总被引：1，自引：0，他引：1  

李明忠  曾举华 《中国兽医寄生虫病》1997,5(4):10-12

为了验证国产杀螨新药12．5％双甲脒乳剂防治獭兔螨病的效果，本试验将12．5％双甲脒乳剂按1：250冲淡配成0．05％浓度，先对五通桥区及雅安市两地各50只病免进行小区典型治疗试验，用药浴、喷淋、涂抹法治疗。结果对怀孕母兔、带仔母兔及仔兔均极安全，治疗7d后镜检，螨虫死亡，痒感消失，体重增加，症状痊愈。7～10d再用同法治疗一次巩固疗效。同时配成0．1％乳剂喷洒兔舍，作环境消毒，连续观察43d，无一只复发。接着在五通桥区和雅安地区大面积推广应用，共治疗病兔93，750只，总有效率达99．12％，取得了满意的治疗效果。  相似文献   

兔胚胎培养液的血小板活化因子效应     

郑行  马从容 《中国兽医学报》1997,17(5):500-503

将兔２细胞期或８～１６细胞期胚胎培养２４ｈ后的胚胎培养液分别注入去脾兔或去脾小鼠体内。注射后１２０ｍｉｎ内，均出现外周血中血小板数的明显减少。此现象是由于胚胎释放的某种活性物质引起的，该活性物质可能是血小板活化因子，经冷冻保存不丧失生物活性，且不存在种属差异  相似文献   

桔皮油对家兔耳(足)螨病治疗试验     

刘俊华  王芳生  曹生福  张国军 《中国兽医学报》1986,(4)

用桔皮油对505只耳(足)螨病兔进行治疗,其中耳螨兔432只,耳、足螨混合感染兔73只,用药后7d病免全部冶愈,60～180d复查,未发现复发病例。离体杀螨试验,桔皮油经过5～10min将螨虫全部杀死。由此证明,桔皮油具有药效快、杀虫率高、对兔体无毒副作用等特点。  相似文献   

Postnatal Osteological Development of the Hyoid Bone in the New Zealand White Rabbit     

Atalgin H  Kürtül I  Bozkurt EU 《Veterinary research communications》2007,31(6):653-660

This study augments knowledge of bone growth by observing the development of the hyoid bone in the New Zealand White rabbit. Preserved hyoid bones representing five different age periods, each period including five individuals and the total number of animals being 25, were fixed in 3.5% formaldehyde solution and 95% ethanol, followed by a pure acetone bath. They were then stained with an alcian blue–alizarin red combination. The mode of bone formation was intracartilaginous type ossification. While the basihyoideum and thyrohyoideum were observed to start ossifying first at prenatal stage, indicating that they are the main skeletal structures of the hyoid apparatus, the ceratohyoideum and lingual process began to ossify in the second and third periods. The separately occurring primary ossification centres fused completely among themselves in the fifth period. Because no further ossification centers were observed and the ossified parts continued the development and growth, the research was terminated after 10 weeks of age.  相似文献   

美国加州葡萄生产与加工现状     

刘大鹏  王文生等 《保鲜与加工》2002,2(5):8-10

对美国加州近年来的葡萄生产和葡萄酒、葡萄干加工现状进行了概括总结,为推动我国葡萄业生产和加工的发展提供理论借鉴。  相似文献   

兔胰腺干/祖细胞的分离培养和鉴定   总被引：3，自引：1，他引：3  

张慧茹  冯若鹏  刘雨潇  庞全海  窦忠英 《农业生物技术学报》2005,13(6):768-771,i0011

采取1～9日龄的实验仔兔胰腺,1 mg/mLⅣ型胶原酶消化,低糖DMEM培养.细胞铺满后进行传代20代以上冻存.对2～3代的细胞进行免疫组化染色,诱导向β细胞分化并进行高糖刺激胰岛素分泌.结果表明,兔的胰腺干/祖细胞具有较强的增殖能力;免疫组化染色表现为PDX-1、CK19、nestin和胰高血糖素阳性;分化诱导2 d后,双硫腙染色（DTZ）20%阳性,6 d后迅速增加到80%;高糖刺激诱导细胞具有胰岛素释放能力.通过免疫组化染色和诱导细胞胰岛素分泌实验证明分离的是兔胰腺干/祖细胞.首次报道兔胰腺干/祖细胞分离培养方法.  相似文献   

Geographic associations of breeding bird distribution in an urban open space     

Daniel S Cooper 《Biological conservation》2002,104(2):205-210

The Puente-Chino Hills, extending west into the highly urbanized Los Angeles Basin, represent one of the largest expanses of lowland habitats in the region. During spring and early summer of 1997 and 1998, birds and vegetation surveys were conducted to clarify the influence of geographical position in the distribution of birds in the hills. Using logistic regression, the inclusion of longitudinal position as a variable is shown to make a statistically significant contribution to bird species presence beyond that of habitat alone for 12 of the 49 most commonly detected species. Species more common than would be expected based on habitat in the east were typical of grassland and open habitats, whereas those more common in the west were characteristic of tall scrub or urban habitats. Thus, species’ distributions in the hills are likely influenced by landscape-scale vegetation patterns and by the aggregate amount of urbanized areas in the west. This emphasizes the importance of using geographical position as a variable when analyzing patterns in bird distribution and siting conservation areas.  相似文献   

不同因素对乳突类圆线虫离体培养的影响     

黄占欣  张毅强 《广西农业生物科学》1995,(1)

采用生物统计的正交试验设计方法，研究了Ｍ１９９、犊血清（ＣＢＳ）、鸡胚提取物（ＣＥＥ５０）、兔肝提取物（ＲＬＥ５０）、丙酮酸钠（ＳＰ）、水解酪蛋白（ＣＨ）和酵母提取物（ＹＥ）在离体培养中对乳突类圆线虫第三期幼虫存活和生长发育的影响。结果表明，只有ＣＢＳ和ＲＬＥ５０。对第三期幼虫的存活和生长发育起着显著的促进作用、在试验得出的最适含量组合的培养基内，幼虫生长发育最好，存活时间最长为３７ｄ。对Ｍ１９９、ＥＭＥＭ、Ｆ１２和ＲＰＭＩ１６４０这４种商品组织培养基进行了筛选试验；结果显示Ｍ１９９最佳，表明脂溶性维生素可能是乳突类圆线虫的离体培养所需营养之一。  相似文献   

Effects of human activity on the foraging behavior of sanderlings Calidris alba     

Kate Thomas  Rikk G Kvitek  Carrie Bretz 《Biological conservation》2003,109(1):67-71

Urbanization and coastal development has dramatically reduced the beach habitat available for foraging shorebirds worldwide. This study tested the general hypothesis that recreational use of shorebird foraging areas adversely affects the foraging behavior of sanderlings Calidris alba. Observations conducted on two central California beaches from January through May and September through December of 1999 showed that number and activity of people significantly reduced the amount of time sanderlings spent foraging. Although the sample size was low, the most significant negative factor was the presence of free running dogs on the beach. The experimentally determined minimal approach distance did not vary significantly with the type of human activities tested. Based on these results, policy recommendations for minimizing the impact of human beach activities on foraging shorebirds include: (1) people maintain a minimum distance of 30 m from areas where shorebirds concentrate and (2) strict enforcement of leash laws.  相似文献   

家兔精子体外获能与体外受精试验   总被引：4，自引：0，他引：4  

徐君  钱菊芬  张涌 《西北农林科技大学学报(自然科学版)》1990,(1):19-24

本试验目的在于建立一种简便而有效的系统,能使家兔射出的精子完成体外获能,并获得体外受精、早期卵裂和正常产仔。精子体外获能采用三种方法:A.HIS(15min)+DM(1～2h);B.HIS(15min)+DM(11～12h);C.m-HIS(15min)+m-DM(2～4h)。经上述方法处理的精子与512枚输卵管卵母细胞进行体外受精和体外培养的结果表明,A组的受精和早期卵裂发育延迟;B组的受精卵大都停留在原核期;C组的受精和早期卵裂发育正常。将C组60枚2-4细胞移植至9只受体兔,5只妊娠,其中2只产4只试管仔兔,另外3只获得12个胎儿。  相似文献