Quality improvement by feeding wild-caught edible crab (Cancer pagurus L.): a pilot study     

Astrid K Woll  Gro I van der Meeren  & Stig Tuene 《Aquaculture Research》2006,37(14):1487-1496

High season for the Norwegian Brown crab fishery (Cancer pagurus L.) is from August to October when meat yield (MY) is high, especially for female crabs. However, the quality of hard‐shelled crabs may vary between and within regions also, at this time of the year. A pilot study was conducted to examine whether feeding could increase MY in wild‐caught medium‐quality females caught during four periods from July to November. Feed intake was compared for crabs fed at different temperatures (3, 5, 8, 10 and 12°C). The crabs were fed three times a week in periods from 15 to 20 days. Feed intake increased with temperatures. Quality, as assessed by MY, was higher for all fed groups compared with reference groups. A clear pattern in quality improvement was seen according to season. Hepatosomatic index increased more than the gonadosomatic in July, slightly more in August, while gonadosomatic development was higher in September. In October, some spawning occurred. The result indicates that feeding had a positive effect, which seemed to increase with increasing temperature. We suggest that the temperature should be at least 12°C in order to achieve an optimal gain in MY when the feeding period is 3 weeks.  相似文献   

What is your diagnosis? Multilobate nasal mass in a 5‐month‐old Sphynx cat          下载免费PDF全文

Veronika Simerdova  Milos Vavra  Misa Skoric  Ivo Hajek  Ondrej Skor 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2017,46(2):369-370

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Doxorubicin induces stemness of mouse TNBC 4T1 cells through Stat3-Oct-4/CD44 signaling pathway     

ZHONG Jin-yi  CHENG Cong-cong  XU Jin-yuan  LI Xue-song  ZHANG Sheng  ZHANG Bao-gang  WANG Li-hua  SHI Li-hong 《园艺学报》2019,35(2):360-364

AIM:To investigate the stemness of mouse triple-negative breast cancer (TNBC) 4T1 cells induced by doxorubicin (DOX) and the underlying mechanism. METHODS:The 4T1 cells and MDA-MB-468 cells were treated with DOX at different concentrations (0, 0.05, 0.1 and 0.5 μmol/L) for 24 h, and the shape and viability of the cells were observed. The concentration of DOX at 0.1 μmol/L was chosen as the optimal concentration for the following experiments. The 4T1 cells and MDA-MB-468 cells resistant to DOX were established by continuous stimulation with DOX for 4 weeks, and named as 4T1-DOX and MDA-MB-468-DOX. Sphere formation assay was used to detect the stemness of 4T1 cells and MDA-MB-468 cells. The expression of CD133 was observed by immunofluorescence staining. The expression of CD44 was analyzed by flow cytometry. The protein levels of Stat3, phosphorylated Stat3 (p-Stat3) and Oct-4 were determined by Western blot. RESULTS:The sphere formation ability of the 4T1-DOX cells was stronger than that of the 4T1 control cells. The 4T1-DOX cells expressed high levels of the stemness markers CD133 and CD44 as compared with the 4T1 cells (P<0.05). Furthermore, the 4T1-DOX cells exhibited enhanced activation of Stat3 (p-Stat3) and increased expression of Oct-4 (P<0.05), while the expression of total Stat3 had no obvious variation. In addition, when activation of Stat3 was inhibited by WP1066, the protein levels of p-Stat3, Oct-4 and CD44 were down-regulated (P<0.05). Furthermore, inhibition of Stat3 phosphorylation reduced the sphere formation ability of the 4T1-DOX cells (P<0.05). CONCLUSION:DOX induces the stemness of mouse TNBC 4T1 cells through Stat3-Oct-4 signaling pathway.  相似文献