Occurrence of reproductive disorders in pig herds with and without Chlamydia suis infection – statistical analysis of breeding parameters          下载免费PDF全文

Krzysztof Rypuła  Aleksandra Kumala  Katarzyna Płoneczka‐Janeczko  Paweł Lis  Ewa Karuga‐Kuźniewska  Krzysztof Dudek  Ireneusz Całkosiński  Patrycja Kuźnik  Paweł Chorbiński 《Animal Science Journal》2018,89(5):817-824

Chlamydiae are frequently encountered Gram‐negative intracellular eubacteria that can cause clear manifestations or clinically asymptomatic disorders. C. suis and other chlamydia are primarily isolated in cases of reproductive disorders. This study was performed to estimate the impact of Chlamydia suis infection on reproduction in sows by analyzing reproduction rates and breeding parameters. The test was conducted on first generation (F1) pigs from Polish Landrace (PL) × Polish Large White (PLW). Sixty‐four herds were investigated and 500 vaginal swabs were collected. Isolation of DNA was carried out directly from the swabs. All samples were analyzed for Chlamydia suis by real‐time PCR with a locked nucleic acid (LNA)‐containing probe. To analyze the impact of chlamydia infection on reproductive parameters, evaluation questionnaires were used. Reproductive problems were found in 77.3% of the farms tested. The receiver operating characteristic curve indicated that in the farms with 10 up to 120 sows, there were higher reproductive problems with chlamydia infection than in smaller and bigger pig farms. The most common problems were estrus repetition, which was reported by 57.81% of the surveyed farms, and the birth of dead piglets, which was reported by 31.25% of the investigated pig farms. Abortions, which were reported by 28.12% of the surveyed farms, were the least common reproductive disorders.  相似文献   

青海省英得尔种羊场种羊流产血清学调查   总被引：1，自引：0，他引：1  

冉双存  靳文忠  李长宏  田小龙  雅航前  董廷贵 《中国动物检疫》2010,27(6):54-55

通过间接血凝试验(IHA)和虎红平板凝集试验(RBPT),对来自青海省英得尔种羊场的353份羊血清进行布鲁氏菌、弓形虫和衣原体三种主要疫病的血清抗体检测。结果表明,18.9%(33/174)的山羊血清和3.9%(7/179)的绵羊血清其衣原体抗体血清凝集效价≥1:16(++),被判为阳性;抗布鲁氏菌和弓形虫的血清抗体均未检测到。  相似文献   

动物鹦鹉热衣原体疫苗研究现状   总被引：1，自引：0，他引：1  

杨琪  何诚  朱虹  端青 《动物医学进展》2004,25(5):8-11

鹦鹉热衣原体为多种人畜共患病的病原体。衣原体免疫学机制研究表明其主要引发Th1细胞调节的细胞免疫和体液免疫。减毒活疫苗、灭活疫苗的使用在一定程度上控制了其发病率。基因工程疫苗,如重组亚单位苗、核酸疫苗、细茵载体苗等也都在深入研究中。同时,人们也在筛选特异性的抗原,构建各种疫苗载体及选择免疫佐剂和免疫途径等方面做了大量的工作。  相似文献   

入境肉种鸡检出衣原体阳性及其处理     

金钺  苗丽  李平  王亚宾  张红英  陈丽颖 《中国动物检疫》2009,26(6):47-49

目的随着加入世界贸易组织,我国海关检疫压力增大,为了维护国家主权和国际声誉,加强出入境检验检疫势在必行。衣原体病是一种重要的人畜共患病,也给养禽业带来巨大的经济损失,必须给予足够的重视,鹦鹉热衣原体对多种动物宿主有广泛的寄生性引起不同动物的多症候群疾病,是世界动物卫生组织动物名录疾病。方法对入境的一批肉种鸡进行抽样检验,按照中华人民共和国出入境检验检疫行业标准用间接血凝检测鹦鹉热衣原体病,做出试验报告。结果经检测出现阳性,肉鸡携带有衣原体病毒。结论根据中华人民共和国进出境动植物检疫法第二章进境检疫第十六条检出二类传染病、寄生虫病的动物,退回或者扑杀,同群其他动物在隔离场或者其他指定地点隔离观察。  相似文献   

Baicalin suppresses expression of Chlamydia protease-like activity factor in Hep-2 cells infected by Chlamydia trachomatis     

Huang Hao  Yang Aixia  li Dan  Fu lei  Yu Nancai  Su Wen 《Fitoterapia》2009,80(7):448-452

In this study, we tested the ability of Baicalin to block Chlamydia trachomatis infection and found that the Baicalin blocked infection of Hep-2 cells. Then, we looked into the expression of RFX5 and CPAF gene in Chlamydia-infected cells. We found that RFX5 and CPAF were up-regulated and down-regulated respectively by Baicalin. Since CPAF is responsible for degrading RFX5, we suggest that CPAF was a primary target of Baicalin and played an important role in regulating RFX5. Our findings demonstrate that Baicalin can inhibit C. trachomatis effectively and therefore, can be considered as potential agents for therapy of Chlamydia infectious diseases.  相似文献   

Seroprevalence of Antibodies to Chlamydophila psittaci in Zoo Workers in Brazil     

T. F. Raso  A. O. T. Carrasco  J. C. R. Silva  M. F. V. Marvulo  A. A. Pinto 《Zoonoses and public health》2010,57(6):411-416

To evaluate the prevalence of antibodies to Chlamydophila psittaci 364 serum samples were collected from veterinarians, biologists, animal scientists, veterinary students, animal keepers and others employees in 20 zoos, and from veterinary practitioners in 10 Brazilian states. Subjects ranged from 15 to 64 years of age, with 268 (74%) males and 96 (26%) females. Chlamydial antibodies were determined by the complement fixation test (CFT) and specific anti‐C. psittaci IgG antibodies were determined by the microimmunoflurescence (MIF) test. Complement fixation test showed 23.9% (87/364) and MIF test showed 4.7% (17/364) positive serum samples. Titres ranged from 16 to 256 in both assays, demonstrating evidence of recent or current infection. Although chlamydial antibodies were detected in workers of seventeen zoos, MIF test only detected specific C. psittaci antibodies in seven of them. Previous psittacosis infection was suspected in eight workers of two zoos, five of whom reported having pneumonia, while employed at the zoos. However, diagnosis was not established in any of these cases in the past. Results indicated the occurrence of infection and previous contact of Brazilian zoo workers with C. psittaci, as well as the zoonotic potential of psittacosis in this risk population. Other studies are necessary to evaluate the risk factors of infection in this population. This seroepidemiological survey confirmed the need to adopt preventive measures to control avian chlamydiosis and protect the health of zoo workers in the country.  相似文献   

广东省东莞市犬、猫衣原体血清学调查     

黄炳炽 《中国兽药杂志》2010,44(7):9-10

应用正向间接血凝试验对东莞市采集的977份犬血清和126份猫血清进行了衣原体抗体检测,检测结果为犬抗体阳性28份,抗体阳性率2.87%;其中散养犬只抗体阳性数24份,抗体阳性率2.96%,三鸟批发市场肉用犬抗体阳性数3份,抗体阳性率3.61%,犬养殖抗体阳性数1份,抗体阳性率1.2%。猫抗体阳性3份,抗体阳性率2.38%。结果表明我市的犬、猫和外地输入东莞市的肉用犬都存在衣原体的感染。  相似文献   

Chlamydia psittaci: a suspected cause of reproductive loss in three Victorian horses     

R Akter  AW Stent  FM Sansom  JR Gilkerson  C Burden  JM Devlin  AR Legione  CM El-Hage 《Australian veterinary journal》2020,98(11):570-573

Chlamydia psittaci was detected by PCR in the lung and equine foetal membranes of two aborted equine foetuses and one weak foal from two different studs in Victoria, Australia. The abortions occurred in September 2019 in two mares sharing a paddock northeast of Melbourne. The weak foal was born in October 2019 in a similar geographical region and died soon after birth despite receiving veterinary care. The detection of C. psittaci DNA in the lung and equine foetal membranes of the aborted or weak foals and the absence of any other factors that are commonly associated with abortion or neonatal death suggest that this pathogen may be the cause of the reproductive loss. The detection of C. psittaci in these cases is consistent with the recent detection of C. psittaci in association with equine abortion in New South Wales. These cases in Victoria show that C. psittaci, and the zoonotic risk it poses, should be considered in association with equine reproductive loss in other areas of Australia.  相似文献   

猪流产鹦鹉衣原体的抗原结构及抗原性和免疫原性分析     

段跃进  杨学军 《畜牧兽医学报》1994,25(6):551-554

本试验用ＳＤＳ－ＰＡＧＥ技术对分离自青海、湖北、广西省流产猪的鹦鹉衣原体抗原结构进行分析，发现这些菌株的抗原结构图谱相似，即共同含有主外膜蛋白ＭＯＭＰ和其他一些主多肽。并以Ｄ１３株免疫兔子制备的抗血清作为探针，用免疫印迹试验检查其与各株鹦鹉衣原体抗原分子的反应关系。证实Ｄ１３株的主要抗原３８ＫＤ（ＭＯＭＰ）、６０ＫＤ、８０ＫＤ和１１０ＫＤ具有抗原性，它们可刺激机体产生抗体免疫应答。该抗血清不仅可与  相似文献   

猪衣原体感染的血清学调查报告     

赵志伟  张山林  郝群  苏玉贤 《河南畜牧兽医(综合版)》2002,23(6):7-7

通过对平顶山地区6个县(市)29个猪场的3000头种猪血清进行鹦鹉热衣原体的间接血凝(IHA)抗体的检测。结果显示,血清的阳性率为3.2%～8%,平均为4.8%,猪场的阳性率为65%,该病在本地区较为普遍。  相似文献