三株不同形状伴孢晶体的苏芸金杆菌对几种害虫的毒力测定     

戴莲韵  麻左力 《林业科学》1989,25(1):29-32

我们从落叶松毛虫(Dendrolimus sibiricus)、印度谷斑螟(Pyralis farinali)幼虫和森林土壤中分离到产生不同形状伴孢晶体的苏芸金杆菌(Bacillus thuringiensis),对三株菌进行了形态特征的电子显微镜观察、生理生化特性的测定及营养细胞的酯酶型分析,并对几种昆虫进行了室内生物测定,表现出不同的毒力。  相似文献   

三种抗旱植物叶片解剖结构的对比观察   总被引：39，自引：0，他引：39  

王怡 《四川林业科技》2003,24(1):64-67

通过制作石蜡切片对沙棘、文冠果、虎榛子叶片的解剖结构进行了观察 ,以角质层厚度 ,栅栏组织厚度/叶肉组织厚度 ,第一层栅栏组织细胞密集度 ,侧维管束密度 ,主脉厚度这几项旱性结构的主要指标进行检验比较 ,结果表明这三种植物都具备抗旱结构但又有各自的抗旱特点 ,综合各抗旱指标对三种植物的抗旱性排序 ,其抗旱能力为 :文冠果 >沙棘 >虎榛子  相似文献   

An enrichment microsphere immunoassay for the detection of <Emphasis Type="Italic">Pectobacterium atrosepticum</Emphasis> and <Emphasis Type="Italic">Dickeya dianthicola</Emphasis> in potato tuber extracts     

Jeroen Peters  Wojciech Sledz  Jan H. W. Bergervoet  Jan M. van der Wolf 《European journal of plant pathology / European Foundation for Plant Pathology》2007,117(2):97-107

An enrichment microsphere immunoassay (MIA) was developed, based on the Luminex xMAP® technology, for the simultaneous (duplex) detection of Pectobacterium atrosepticum (former name Erwinia carotovora subsp. atroseptica) (Pca) and Dickeya dianthicola (former name Erwinia chrysanthemi) (Dcd) in potato plant extracts. Target bacteria in the extracts were enriched for 48 h in a semi-selective broth containing polypectate under low oxygen conditions. Samples were subsequently incubated with antibody-coated colour-coded microspheres (beads) and with secondary antibodies conjugated with Alexa Fluor® 532, a reporter dye. Samples were analyzed with the Luminex analyzer, in which one laser identified each microsphere and another laser the reporter dye conjugated to the secondary antibodies. The assay required minimal sample preparation, could be completed in 1 h, was performed in 96 wells microtitreplates and required no wash steps. The limit of detection for the duplex enrichment MIA was 100–1000 cfu ml^?1, which was a hundred times lower than of an enrichment-ELISA. Without enrichment, the sensitivity of MIA and ELISA was largely similar and ranged between 10⁶ and 10⁷ cells ml^?1. No difference in sensitivity was found between a MIA in a single or duplex format. In a comparative test with non-infected potato plant extracts and extracts from plants infected with Pca or Dcd, results of the enrichment MIA correlated well with those of the enrichment ELISA and enrichment PCR. These results indicate that MIA can be reliably used for multiplex detection of soft rot Enterbacteriaceae in crude potato plant extracts. The technology is an attractive and cost-effective alternative to other detection methods, including ELISA.  相似文献   

中国沙棘克隆生长对不同光照强度的响应     

唐翠平  郭峰  徐德兵  贺斌  李甜江  李根前 《云南林业科技》2014,(3):123-128

利用遮阳网产生的不同光照强度（100％、70％、50％和20％的自然光照）,研究了中国沙棘克隆生长对不同光照强度的响应规律。结果表明,（1）当光照强度从20％逐渐增加到100％时,种群生长量、生物量积累与分配、地下克隆器官参数等增幅均呈二次抛物线变化,相对光照强度为50％～70％是其克隆生长的最佳光照强度。（2）种群生长量、种群及各器官生物量、子株数量及萌蘖根生长量增幅最大时的光强分别为48.0％～61.7％、52.8％～53.6％、55.6％～57.6％,且分株生长量和种群生物量、萌蘖根生长量和子株数量之间具有协同作用,而营养生长和克隆繁殖之间存在权衡关系。（3）在适宜的光照强度下,种群以分株比较高大且分布相对密集为特征,生长格局倾向于集团型;在光照强度过大或过小的情况下,种群以分株比较矮小且分布相对稀疏为特征,生长格局倾向于游击型。当光照强度由低到高变化时,生长格局将由游击型到集团型再到游击型,形成一个觅养对策连续体。  相似文献   

玉米细菌性枯萎病和玉米内州萎焉病的酶联免疫—实时荧光PCR检测     

陈曦  张明哲  林晓佳  吴志毅  陈吴健  吴蓉  夏拯 《浙江农业学报》2014,(5)

针对玉米细菌性枯萎病和玉米内州萎焉病这两种检疫性病菌进行酶联免疫—实时荧光PCR检测研究,对该方法进行了特异性、灵敏度以及实际样品模拟试验。结果表明,该方法具有较好的特异性,检测低限在100~200 cfu·mL-1,同时可用于实际样品的检测鉴定。  相似文献   

β-1,3-葡聚糖及其羧甲基衍生物对海湾扇贝免疫功能的影响   总被引：3，自引：0，他引：3  

杨文鸽 《水产学报》2006,30(3):383-389

2003年3月和2004年4月，新疆某养殖场和西安灞桥区某养殖场的白斑狗鱼（Esox lucius Linnaeus）相继爆发“败血症”。从具有明显症状的濒死病鱼体表病灶和内脏组织分离得到12株优势菌株，经人工感染试验表明：其中10株分离菌为致病菌。对分离菌株进行形态、培养特征、生理生化试验鉴定，认为该病原菌为杀鲑气单胞菌史氏亚种（Aeromonas salmonida subsp．smith）。血清学试验表明各分离菌株具有相同的保护性抗原。组织病理学研究表明：患病狗鱼病灶肌纤维明显肿胀断裂，肌纤维束之间出现间隙；肝胰组织严重坏死，细胞核溶解消失，细胞浆崩解；肾小管绝大部分均已坏死，细胞破裂，胞浆呈红色团块状流失于管腔。脾脏细胞大量坏死，髓质内含铁血黄素沉积。代表菌株EL0304-1药敏试验结果为：对供试24种抗菌药物中的磺胺二甲嘧啶钠、磺胺二甲嚯唑钠等不敏感；庆大霉素、卡那霉素等中度敏感；对氧氟沙星、培氟沙星、新霉素等高度敏感。  相似文献   

Detection of quinolone-resistance genes in Photobacterium damselae subsp. piscicida strains by targeting-induced local lesions in genomes     

Kim MJ  Hirono I  Aoki T 《Journal of fish diseases》2005,28(8):463-471

Quinolone-resistant strains of the fish-pathogenic bacterium, Photobacterium damselae subsp. piscicida are distributed widely in cultured yellowtail, Seriola quinqueradiata (Temminck & Schlegel), in Japan. The quinolone resistance-determining region (QRDR) was amplified with degenerate primers, followed by cassette ligation-mediated PCR. Open reading frames encoding proteins of 875 and 755 amino acid residues were detected in the gyrA and parC genes, respectively. Resistant strains of P. damselae subsp. piscicida carried a point mutation only in the gyrA QRDR leading to a Ser-to-Ile substitution at residue position 83. No amino acid alterations were discovered in the ParC sequence. A mutation in the gyrA gene was also detected in nalidixic acid-resistant mutants of strain SP96002 obtained from agar medium containing increased levels of quinolone. These results suggest that GyrA, as in other Gram-negative bacteria, is a target of quinolone in P. damselae subsp. piscicida. Furthermore, we attempted to detect a point mutation using targeting-induced local lesions in genomes (TILLING), which is a general strategy used for the detection of a variety of induced point mutations and naturally occurring polymorphisms. We developed a new detection method for the rapid and large-scale identification of quinolone-resistant strains of P. damselae subsp. piscicida using TILLING.  相似文献   

马链球菌马亚种新疆株ZX表面蛋白的提取及免疫原性分析     

王国勤  姜慧娇  江航  苏艳 《新疆农业大学学报》2014,(3):181-184

为研究马链球菌马亚种新疆分离株 ZX 表面蛋白的免疫原性,用化学法提取该菌的表面蛋白,用 SDSG PAGE检测提取的表面蛋白,并将提取的表面蛋白与该菌免疫实验兔后产生的抗体及康复马血清反应,利用 WestG ernGblotting和 ELISA试验分析其免疫原性.结果表明,SDSGPAGE电泳可检测到分子量为45,34,33,30,27,20,16 kDa 7条主蛋白带.提取的7条蛋白带多数可与全菌免疫兔的抗体及康复马体内的抗体发生特异性的结合.用提取蛋白和全菌包被 ELISA对马血清的检测表明,二者的阳性吻合率在86．0％.表明提取的表面蛋白具有理想的免疫原性.  相似文献   

尾叶拉藤多糖含量测定     

高伟略  田仕勇  彭小冰  危莉  邵进明  杨茂忠  危英 《山地农业生物学报》2014,(5):92-94

本文采用苯酚-硫酸比色法,测定尾叶拉藤中多糖的含量。结果表明尾叶拉藤中多糖含量为3.85%,平均加样回收率101.38%。  相似文献   

凹叶厚朴叶的生长规律与开发利用   总被引：3，自引：0，他引：3  

史小刚  肖双燕 《江西林业科技》2001,(4):1-2,8

凹叶厚朴既是传统的药用植物，又是具备保健功能的食品资源。本文观察研究了凹叶厚朴叶的物修、生长规律、生物量、化学成分和浓缩汗的工艺小试，为可持续利用植物资源提供参考。  相似文献