乳酸乳球菌乳脂亚种IMAU60064增殖培养基的优化     

徐洁  张青  杨洁  薛建岗  伊利那·塞日波纳·哈玛耶娃  张家超  陈永福  张和平 《乳业科学与技术》2011,34(4):151-157

以分离自西藏那曲县罗玛镇传统发酵酸牦牛奶中的乳酸乳球菌乳脂亚种（Lactococcus lactis subsp．Cremoris）IMAU60064为试验菌株,研究其最佳的培养条件。对碳源、氮源、缓冲盐等培养基成分及培养条件进行优化,并采用响应面法对优选的碳源、氮源和缓冲盐类的组成含量进行优化,得到IMAU60064的增殖培养基为：葡萄糖23g／L、大豆蛋白胨11g／L、牛肉膏11g／L、胰蛋白胨5g／L、NaAC1．8g／L、K2HP041．2g／L、柠檬酸钠1．2g／L、MgSO4·7H200．4g／L、MnSO4·5H2O54mg／L、L-半胱氨酸盐酸盐0．5g/L、吐温80为1g／L。Lactococcus lactis subsp．cremorisIMAU60064在此增殖培养基中经30℃,14h培养活菌数可达到3．26×10^8cFu／mL,比在MRS中（6．54×10^7CFU／mL）提高近5倍。  相似文献   

三种原产中国的野生稻和栽培稻外稃表面乳突结构的比较观察研究   总被引：11，自引：1，他引：11  

汤圣祥  张文绪 《中国水稻科学》1996,10(1):19-22

 用扫描电镜对原产中国的瘤粒野生稻（Oryza meyeriana Baill. subsp. tuberculata Wu），药用野生稻（Oryza officinalis Wall. ex Watt.），普通野生稻（Oryza rufipogon Griff. ）的外稃表面的显微结构与栽培稻（Oryza sativa L.）进行了比较研究。观察到栽培稻与瘤粒野生稻在一系列性状上差异最大，其次是药用野生稻，而最相似的是普通野生稻。应用性状数码的方法对种间的相似性作了比较。  相似文献   

Differential clustering of Mycoplasma mycoides subsp. mycoides SC strains by PCR-REA of the bgl locus     

Vilei EM  Frey J 《Veterinary microbiology》2004,100(3-4):283-288

In order to develop a specific tool differentiating the African field strains of Mycoplasma mycoides subsp. mycoides SC from other potentially less virulent strains, including the vaccine strains, we have developed a PCR followed by a restriction enzyme analysis (PCR–REA). This approach also differentiates the African field strains from the Australian strains and the type strain PG1. The genomic marker detected by the PCR–REA is based on a single nucleotide change in the bgl gene that codes for 6-phospho-β-glucosidase (Bgl), an enzyme that is involved in sugar metabolism.  相似文献   

In vitro susceptibility of field isolates of Francisella tularensis subsp. holarctica recovered in Spain to several antimicrobial agents     

García del Blanco N  Gutiérrez Martín CB  de la Puente Redondo VA  Rodríguez Ferri EF 《Research in veterinary science》2004,76(3):195-198

Forty-two recent (1997-1999) Spanish isolates of Francisella tularensis subsp.holarctica were tested in a broth microdilution method for their susceptibilities to 29 antimicrobial agents, including penicillins, cephalosporins, cephamicins, monobactams, penems, aminoglycosides, tetracyclines, macrolides, quinolones, chloramphenicol and fosfomycin. The isolates were resistant to beta-lactam antibiotics and susceptible to chloramphenicol, ciprofloxacin, levofloxacin and norfloxacin.  相似文献   

Characterisation of protein and antigen variability among Mycoplasma mycoides subsp. mycoides (LC) and Mycoplasma agalactiae field strains by SDS-PAGE and immunoblotting     

de la Fe C  Assunção P  Rosales RS  Antunes T  Poveda JB 《Veterinary journal (London, England : 1997)》2006,171(3):532-538

Mycoplasma mycoides subsp. mycoides (LC) (Mmm LC) and Mycoplasma agalactiae are the most important mycoplasma species involved in the contagious agalactia syndrome. A total of 25 field strains from Spain and the two type strains were analysed by SDS-PAGE and immunoblotting. Two polyclonal antisera (PAbs) raised against a pool of strains of each mycoplasma species were used. The results revealed a high degree of protein variability among the field strains. The type strain of Mmm LC appeared to be representative of the field strains of this species, whereas this was not the case with the M. agalactiae type strain. Whereas M. agalactiae is known to possess a gene family regulating surface antigen diversity, there is a need to study the mechanisms used byMmm LC to generate antigenic variability in more detail.  相似文献   

菜豆普通细菌性疫病菌在土壤和植株残体中的越冬能力   总被引：1，自引：1，他引：0  

徐新新  陈鸿宇  王述民  段灿星  朱振东 《植物保护学报》2012,39(3):231-236

为评估菜豆普通细菌性疫病菌地毯草黄单胞杆菌菜豆致病变种或褐色黄单胞菌褐色亚种在土壤及植物残体中的越冬能力,对采自黑龙江、内蒙古、山西、河北及新疆的18块菜豆生产田的20份土壤及14份植物残体样品进行病原菌分离和鉴定。在MT选择性培养基上有12个土壤样品和13个植株残体样品提取液产生典型的类似黄单胞菌菌落。选取29个分离物进行致病性测定,有27个分离物对菜豆品种"英国红"致病。利用地毯草黄单胞杆菌菜豆致病变种和褐色黄单胞菌褐色亚种的特异性引物X4c/X4e及褐色黄单胞菌褐色亚种特异性引物Xf1/Xf2对29个分离物进行多重PCR检测,其中17个分离物为地毯草黄单胞杆菌菜豆致病变种,10个分离物为褐色黄单胞菌褐色亚种。结果表明,菜豆普通细菌性疫病菌可以在黑龙江、内蒙古、山西、河北的一些菜豆种植区的土壤及植株残体中越冬存活。  相似文献   

番茄溃疡病菌的PCR-DHPLC快速检测     

吴兴海  陈鹏  左一鸣  张成标  封立平  魏晓棠 《植物检疫》2012,(1):18-23

根据番茄溃疡病菌ITS序列,设计并合成了PCR-DHPLC检测引物,对番茄溃疡病菌及其他病菌共10个标准菌株进行了PCR-DHPLC检测。结果表明,番茄溃疡病菌的PCR-DHPLC检测图谱出现了特异性吸收峰,而其他病菌均未在相同洗脱时间出现吸收峰,说明这种方法具有检测番茄溃疡病菌的特异性。灵敏度实验结果表明,PCR-DHPLC体系与PCR-琼脂糖凝胶电泳体系的检测灵敏度一致。研究表明,PCR-DHPLC方法是一种特异、灵敏、快速的番茄溃疡病菌检测方法。  相似文献   

Influence of Mycorrhizal Inoculation on the Salt Tolerance of Artichoke Hybrid Seedlings     

Angela Campanelli  Claudia Ruta  Irene  Morone-Fortunato Giuseppe  De Mastro 《农业科学与技术》2012,(9):1071-1079

  相似文献   

记述中国蝴蝶新记录亚种     

刘家柱  周雪松 《湖南林业科技》2013,(1):18-19,126

记述了中国2个蝴蝶新记录亚种,其中兰带枯叶蛱蝶云南亚种为枯叶蛱蝶属,采自云南省腾冲高黎贡山,研究标本保存于西南林业大学标本馆;云南矩环蝶马鞍底亚种为矩环蝶属,采自云南省红河州金平县马鞍底乡,研究标本保存于马鞍底中国·红河蝴蝶谷展览馆。  相似文献   

津田芜菁隐花色素CRY1 RNA干涉载体的构建     

孙梅  周波  马光  刘明雪  李玉花 《分子植物育种》2013,(3):437-442

隐花色素CRY1在拟南芥光形态建成及光信号转导中起重要作用,在津田芜菁(Brassica rapaL. subsp. rapa ‘Tsuda’)中是否起相同作用尚未报道。本研究利用实验室已克隆得到的津田芜菁CRY1基因的cDNA全长序列,在NCBI进行序列比对,选取特异的片段。同时根据Gateway克隆技术特点,设计含有attB接头的引物。利用高保真的LA Taq DNA聚合酶,通过PCR方法在目的片段的两端加上attB序列。通过BP反应和LR反应将CRY1基因片段克隆到pH7GWIWG2(I)双元干涉载体。对重组载体pH7GWIWG2(I)-CRY1的鉴定结果表明成功构建了CRY1基因的干涉载体。利用Gateway克隆技术构建植物干涉表达载体简便易行,该结果为遗传转化研究其功能奠定了基础。  相似文献