A streak disease of pearl millet caused by a leafhopper-transmitted geminivirus     

Rob W. Briddon  Patricia Lunness  Ian D. Bedford  Leony C. L. Chamberlin  Theo Mesfin  Peter G. Markham 《European journal of plant pathology / European Foundation for Plant Pathology》1996,102(4):397-400

The cause of a streak disease of pearl millet (Pennisetum glaucum), originating from Nigeria, has been attributed to a geminivirus belonging to the African streak virus cluster. A full-length, infectious clone of the virus was obtained which was transmissible by the vectorCicadulina mbila (Naudé). Analysis of the complete nucleotide sequence of the coat protein gene of this virus shows it to be most closely related to sugarcane streak virus. The possible evolutionary implications of this finding are discussed.  相似文献   

Characterisation of a maize-infecting potyvirus from Spain     

M. Angeles Achon  Vicente Medina  Michael Shanks  Peter Markham  George P. Lomonossoff 《European journal of plant pathology / European Foundation for Plant Pathology》1994,100(2):157-165

In order to characterise and classify an unknown maize-infecting potyvirus isolated from fields in northeast Spain, the entire coat protein gene and the C-terminal twothirds of the large nuclear inclusion protein (NIb) gene were cloned and sequenced. Protein sequencing enabled the cleavage site between the two proteins to be deduced and also revealed that on storage the viral coat protein undergoes a specific degradation in which the N-terminal 39 amino acids are removed. Comparison of the nucleotide sequence of the 3 non-coding region of the viral RNA and the predicted amino acid sequence of the coat protein with the equivalent regions of other members of the potyvirus group revealed that the Spanish virus is closely related to maize dwarf mosaic virus strain A.  相似文献   

关联度分析在农业生态系统技术调控上的应用     

文化  张壬午 《华北农学报》1990,5(3):94-99

农业生态系统是个“灰箱”.我们运用模糊数学的关联度分析,尝试对这类灰色系统的投入进行技术调控,并取得了良好的效果.通过对农田子系统投入的化肥(尤其是N化肥)、粪肥和还田秸秆进行技术调控的实例证明;它可以作为能、物流分析的辅助手段,互相取长补短.通过关联序分析和关联系数分析,来调整系统的投入结构和投入技艺(时、空、量级及方式的选择).本文提出了应用关联度分析的基本程序和要领以及“贡献曲线”、“潜力曲线”等概念.  相似文献   

应用灰色关联度分析法综合评价高羊茅品种初探     

田伯红徐玉鹏  李雅静刘全凤王秀领  赵忠祥王静 《四川草原》2005,(9):18-20,40

应用灰色关联度分析法,对2000～2002年本院引进的13个高羊茅品种的适应性鉴定结果进行评估,其评估结果与品种的实际表现基本一致,说明应用关联度分析法综合评价草坪品种是可行的。分析结果:皇后、贝克、上南都的关联系数与理想品种最接近,是种植高羊茅草坪的首选品种。  相似文献   

Growth loss of Lebanon cedar (Cedrus libani) stands as related to periodic outbreaks of the cedar shoot moth (Dichelia cedricola)     

Serdar Carus  Mustafa Avcı 《Phytoparasitica》2005,33(1):33-48

An outbreak ofDichelia cedricola (Diakonoff) (Lep.: Tortricidae), the cedar shoot moth (CSM), began in spring 1998 and lasted 3 years. This was the first monitored outbreak of the CSM in Isparta, Turkey. Tree crowns recovered to near normal condition by the middle of each growing season (in early June) during the outbreak. Tree volume and volume element increments were examined throughout the outbreak cycle from 1954 to 2001. In the past, CSM activity in stands of Lebanon cedar (Cedrus libani A. Rich.) was assessed through radial increment analyses. Cedar tree ring chronologies were analyzed for evidence of the CSM. Tree-ring chronologies from nonhost cedar (nondefoliated sample trees) were used to estimate potential growth in the host cedar (defoliated sample trees) during current and past outbreaks; all trees selected were the same subspecies and varieties. Regional outbreaks of the CSM were identified by synchronous and sustained growth periods of the trees. In 2001, increment cores were collected from 17 host and 16 nonhost dominant or codominant trees and annual radial growth indices from 1954–2001 were calculated for each of two host and two nonhost sample plots. Growth functions were defined as the cumulative sum of radial, height, and volume increment, and were graphically compared between CSM host cedar and nonhost cedar trees. Tree ring evidence suggests that a large-scale outbreak occurred in 1955 (from 1955 to 1966) and a small outbreak occurred in 1985 (1985–1990) and in 1998 (1998-continued) in the study area. The average diameter growth reductions around 1955, 1985 and 1998 were 40%, 46% and 7% of potential, respectively. It was concluded that a narrow latewood band is significant indicator of defoliation by the CSM and the outbreaks appear to be associated with dry winter and spring weather prior to the autumn and winter in which wood feeding occurred. http://www.phytoparasitica.org posting Nov. 24, 2004.  相似文献   

Enantioselective degradation kinetics of metalaxyl in rabbits     

Jing Qiu 《Pesticide biochemistry and physiology》2005,83(1):1-8

Metalaxyl [methyl-N-(2′-methoxyacetyl)-N-(2,6-dimethylphenyl)-d,l- alaninate] is a potent phenylamide fungicide. The (−)-(R)-isomer accounts for most of the fungicidal activity. A possible stereo and/or enantioselective kinetics of metalaxyl in rabbits was investigated by intravenous injection. The concentrations of (−)-(R)- and (+)-(S)-metalaxyl in plasma, liver, and kidney tissue were determined by HPLC with a cellulose-Tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase and gas chromatography-mass spectroscopy. After intravenous administration of racemic metalaxyl (40 mg/kg), the (+)-(S)-enantiomer levels in plasma, liver, and kidney decreased more rapidly than the (−)-(R)-isomer. The area ratio of the (−)-(R)-/(+)-(S)-enantiomer under the concentration-time curve (AUC_0 → ∞) in plasma after drug application was 1.62. The total plasma clearance value of the (+)-(S)-enantiomer was 1.53 and higher than that of the (−)-(R)-enantiomer. The [R]/[S] ratio in plasma was >1 for standard rac-metalaxyl at each time point. The other pharmacokinetic parameters of the enantiomers were also different. The results indicate substantial stereoselectivity in the degradation of metalaxyl enantiomers in rabbits.  相似文献   

Molecular cloning, expression, and characterization of a phi-type glutathione S-transferase from Oryza sativa     

Hyun-Young Cho 《Pesticide biochemistry and physiology》2005,83(1):29-36

The structural gene for glutathione S-transferase in Oryza sativa was successfully cloned from a cDNA library by the polymerase chain reaction method. The deduced amino acid sequence of this gene showed 44-66% similarity to the sequences of the class phi GSTs from Arabidopsis thaliana and Zea mays. This gene was expressed in Escherichia coli with the pET vector system and the gene product was purified to homogeneity by GSH-Sepharose affinity column chromatography. The expressed OsGSTF3-3 was a homo-dimer composed of 24 kDa subunit and its pI value was approximately 7.3. The OsGSTF3-3 was retained on GSH affinity column and its K_m value for GSH was 0.28 mM. The OsGSTF3-3 displayed high activity toward 1-chloro-2,4-dinitrobenzene, a general GST substrate and also had high activities towards acetanilide herbicides, alachlor, and metolachlor. The OsGSTF3-3 was highly sensitive to inhibition by benastatin A and S-hexyl-GSH. From these results, the expressed OsGSTF3-3 is a phi class GST and seems to play an important role in the conjugation of the chloroacetanilide herbicides.  相似文献   

食药用菌多糖的提取、分离纯化与结构分析   总被引：5，自引：0，他引：5  

张安强  张劲松  潘迎捷 《食用菌学报》2005,12(2):62-68

随着对食药用菌多糖研究的不断深入，多糖的生物活性引起了人们的广泛关注，揭示多糖的生物活性已显得愈来愈重要。本文综述了食药用菌多糖提取、分离纯化与结构分析方面的技术与方法。  相似文献   

杨属( Populus)黑杨组(Aigeiros)种(品种)间核型比较     

张守攻  齐力旺  韩素英  陈成彬  李秀兰  宋文芹  陈瑞阳 《园艺学报》2005,32(1):70-73

 对杨属黑杨组(Aigeiros) 的15个种(品种) 核型进行了分析, 结果如下: 箭杆杨( Populusnigra var. thevestina) , 2n = 38 = 3M + 29m + 5 sm + 1 st; 盖杨( P. del.cv. ×Lux ×P.×canadensis cv. Shanhaiguanensis) , 2n = 38 = 2M + 27m + 4 sm + 5 st (4SAT) ; 欧美杨107 (P1 ×euram ericana cv. ‘74 /76’) , 2n = 38 = 1M + 29m (1SAT ) + 5 sm + 3 st (3SAT) ; 欧美杨13 ( P. ×euramericana CL 13) , 2n = 38 = 3M + 23m + 6 sm + 3 st (1SAT ) + 3 t (3SAT) ; 加杨( P. ×canadensis Moench) , 2n = 38 = 1M + 27m + 6 sm + 4 st ( 4SAT) ; 辽杨×美洲黑杨(P.maximowiczii Henry ×P.deltoids Bartr. ) , 2n = 38 = 1M + 24m + 9 sm (2SAT ) + 4 st; 中尚8号( P. ×Zhongshangnensis) , 2n = 38 = 4M + 27m (1SAT ) + 2 sm + 3 st (2SAT ) + 2 t; 北京杨( P. ×beijingensis) , 2n = 38 = 3M + 28m + 3 sm + 4 st (2SAT) ; 鲁山杨( P. ×Liaoningensis) , 2n = 38 = 1M + 27m + 3 sm + 6 st (1SAT ) + 1 t (1SAT) ; 廊坊1号( P. CL ‘Langfang 1’) , 2n = 38 = 3M + 28m + 3 sm + 4 st (2SAT) ; 圣山杨(盖杨类) (P. ×gaixianesis) , 2n = 38 = 2M + 27m + 4 sm (1SAT ) + 4 st (3SAT ) + 1 t; 抗- 2 〔P. deltoids CL 2 (GMO) 〕, 2n = 38 = 26m + 7 sm (2SAT ) + 4 st (1SAT ) + 1 t (1SAT) ; 群改3号( P. ×Qungainensis 3) , 2n = 38 = 1M + 27m + 8 st (2SAT ) + 2 t (2SAT) ; 中金2号( P. ×Zhongjinnensis 2) , 2n = 38 = 1M + 18m + 13 sm (1SAT ) + 5 st (2SAT) + 1 t (1SAT) ; 辽宁杨( P. ×eur. cv. San Martino ×P. ×canadensis cv. Shanhaiguanensis) , 2n = 38 = 2M + 26m + 6 sm + 4 st。黑杨各种(品种) 核型均为2B型, 种(品种) 间核型差异主要表现在中部着丝点(M、m) 染色体数目(25～32) 和端部着丝点( st、t) 染色体数目变化(1～6) 上, 核型特征为种(品种) 鉴定提供了一定参考。  相似文献   

香蕉ACC氧化酶基因(MAO3)的克隆及其表达特性分析   总被引：3，自引：0，他引：3  

黄俊生  王华  张世清 《园艺学报》2005,32(5):807-811

 根据同源扩增得到香蕉(Musa acuminata) ACC氧化酶基因(MAO3) 的核心部分, 再通过3'和5'RACE扩增上、下游序列以及Genome-Walker的方法得到启动子部分, 共获得3 718 bp长度的序列。将所得结果进行聚类分析, 发现香蕉中ACC氧化酶的氨基酸序列非常保守, 各序列间的同一性高达99% ,与单子叶植物和双子叶植物中ACC氧化酶的氨基酸序列的同源性在66.7%～71.8%之间。组织原位杂交试验表明MAO3基因的表达具有组织特异性, 初步认为是在韧皮部筛管组织中特异表达。运用实时荧光定量PCR技术, 实时监测到了MAO3基因和香蕉乙烯受体基因ERS2受机械伤诱导的定量变化。  相似文献