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11.
2019年11月底,湖北某商品猪场保育猪只出现厌食、消瘦、呼吸困难、咳嗽等症状,偶有病猪急性死亡,为查找病因,控制疫情,减少损失,依次通过流行病学调查、病理剖检和实验室检测,最终确定该发病猪场为感染猪瘟病毒,进而继发感染Hps-4混合感染,并伴随蛔虫感染导致。通过筛选敏感药物治疗,优化生产管理,成功控制了此次疫情。该案例对此次疫情进行了分析总结,就加强生物安全建设,优化养殖管理,实现生产质量可控提供了参考建议。也为猪瘟混合感染呼吸系统细菌性疾病的防控提供参考。 相似文献
12.
猪繁殖与呼吸综合征·猪瘟和猪圆环病毒Ⅱ型混合感染的鉴定及病原特性分析 总被引：3，自引：0，他引：3
[目的]鉴定由猪瘟病毒（CSFV）、猪繁殖与呼吸综合征病毒（PRRSV）、猪圆环病毒Ⅱ型（PCV2）引发的猪疫病并进行病原特性分析。[方法]采集湖南湘潭（编号为HN/XT）发病猪的组织和脏器,进行DNA、RNA提取,然后进行PCR扩增、测序;同时采用细胞分离技术进行毒株的分离、鉴定。[结果]测序分析结果表明,CSFV、PRRSV和PCV2与目前流行毒序列及GenBank下载序列氨基酸同源性分别为90%、94%和96%左右,可见该次猪病疫情至少是由CSFV、PRRSV和PCV23种病毒混合感染引起。[结论]该研究可对当前危害养猪业混合感染疾病的流行病学研究和净化控制提供数据。 相似文献
13.
V.S. Mavrogianni E. Papadopoulos S.A. Spanos A. Mitsoura S. Ptochos D.A. Gougoulis M.S. Barbagianni I. Kyriazakis G.C. Fthenakis 《Research in veterinary science》2014
Objective was to investigate if trematode infections predispose ewes to mastitis and/or metritis. We used 80 trematode-infected ewes: primigravidae in group P-A and multigravidae in M-A remained untreated, primigravidae in P-B and multigravidae in M-B were drenched with netobimin and multigravidae in M-C were given rafoxanide. We collected faecal samples for parasitological examination, blood samples for β-hydroxybutyrate concentration measurement and uterine content, teat duct material and milk samples for bacteriological examination. We found significant differences in blood β-hydroxybutyrate concentrations between M-A, M-B and M-C during pregnancy (P ? 0.002). We did not observe significant differences between groups regarding development of metritis (P > 0.83). We found that for M-A, M-B and M-C ewes, respectively, median time to first case of mastitis was 5.75, 21 and 6.75 days after lambing (P = 0.003) and incidence risk of mastitis was 0.308, 0.069 and 0.222 (P = 0.047). We postulate that trematode infections predispose ewes to mastitis; perhaps, increased β-hydroxybutyrate blood concentrations adversely affect mammary cellular defences. This is the first report associating parasitic infections with mastitis in sheep. 相似文献
14.
Zhang X Jiang S Wu J Zhao Q Sun Y Kong Y Li X Yao M Chai T 《Veterinary microbiology》2009,133(3):252-256
The co-infection of duck circovirus (DuCV) with Riemerella anatipestifer (RA) or/and Escherichia coli (E. coli) or/and duck hepatitis virus I (DHV-I) in Cherry Valley ducks in China's Shandong Province was investigated by using polymerase-chain-reaction (PCR)-based methods. For this study, 742 ducks sampled at random from 70 duck farms during 2006-2007 were examined using PCR and dot-blot hybridisation (DBH) tests. Overall the DuCV infection rate was 33.29%. Compared with those at 2 weeks of age, the ducks at 3-4 weeks of age were more susceptible to DuCV infection. Compared with the DuCV-negative ones, the DuCV-positive ducks had a higher rate of infection by DHV-I (25.5% vs. 7.475%), RA (23.48% vs. 8.28%) and E. coli (16.19% vs. 4.85%). This investigation shows that DuCV infection is common in Cherry Valley ducks on some farms in Shandong Province. 相似文献
15.
[目的]鉴定由猪瘟病毒(CSFV)、猪繁殖与呼吸综合征病毒(PRRSV)、猪圆环病毒Ⅱ型(PCV2)引发的猪疫病并进行病原特性分析。[方法]采集湖南湘潭(编号为HN/XT)发病猪的组织和脏器,进行DNA、RNA提取,然后进行PCR扩增、测序;同时采用细胞分离技术进行毒株的分离、鉴定。CSFV和PCV2的鉴定:间接免疫荧光法操作。PRRSV的鉴定:由于PRRSV可致Marc-145细胞发生病变,故可由细胞病变与否作出初步鉴定。[结果]测序分析结果表明,CSFV、PRRSV和PCV2与目前流行毒序列及GenBank下载序列氨基酸同源性分别为90%、94%和96%左右,可见该次猪病疫情至少是由CSFV、PRRSV和PCV23种病毒混合感染引起。[结论]该研究可对当前危害养猪业混合感染疾病的流行病学研究和净化控制提供数据。 相似文献
16.
Impact of porcine group A rotavirus co-infection on porcine epidemic diarrhea virus pathogenicity in piglets 总被引：2，自引：0，他引：2
Porcine epidemic diarrhea virus (PEDV) and porcine group A rotavirus (PGAR) are the main causative agents of acute diarrhea in piglets. In South Korea, PGAR is prevalent in piglets naturally infected with PEDV. Piglets naturally co-infected with PEDV and PGAR appeared to have severe and prolonged diarrhea that was distinct from that commonly observed. The aim of this study was to determine the impact of PGAR co-infection on PEDV pathogenicity in piglets. Thirty-six colostrum-deprived, one-day old, Large White-Duroc crossbred pigs were randomly divided into four equal groups: PEDV, PEDV/PGAR, PGAR, and control groups. The piglets were euthanized at 1, 2, or 3 days post-inoculation (DPI) to measure the villous height:crypt depth (VH:CD) ratio and to collect fecal samples for RT-PCR and virus isolation. No significant differences in mean VH:CD ratio and clinical symptoms (diarrhea, vomiting, dehydration, and anorexia) were observed between the PEDV/PGAR-infected and PEDV-infected groups of piglets at 1, 2 and 3 DPI; however, at 2 and 3 DPI, PGAR was detected in all fecal samples by RT-PCR and virus isolation. These findings failed to detect any interaction between PEDV and porcine rotavirus in the small intestines of piglets, suggesting that concurrent infection of PGAR may not synergistically enhance intestinal villous atrophy of piglets with PEDV disease. We propose that the severe diarrhea exhibited in PEDV and PGAR co-infected piglets may be more associated with the immunity level of the host rather than to any synergistic effect of PGAR on PEDV enteritis. 相似文献
17.
A novel avian polyomavirus was detected in peri-ocular skin lesions collected from a grey butcherbird (Cracticus torquatus), using a combination of multiply primed rolling circle amplification, nested PCR and long range PCR. The sequence of Butcherbird polyomavirus was determined by combining next generation sequencing and primer walking techniques. The circular double-stranded DNA genome of Butcherbird polyomavirus consisted of 5084 bp, and encoded six open reading frames (ORF-X, VP2, VP3, VP1, small T-antigen and large T-antigen). Phylogenetic analysis placed it amongst other members of the genus Avipolyomavirus, most closely related to Crow polyomavirus. Next generation sequencing enabled the detection of DNA fragments similar to, but distinct from, Canarypox virus within the same lesion from which Butcherbird polyomavirus was amplified, thus confirming an avipolyomavirus-avipoxvirus co-infection in the peri-ocular skin lesions of this grey butcherbird. 相似文献
18.
山东某父母代肉种鸡开产后死淘率突然升高,死亡鸡肝脏、脾脏、腺胃等内脏器官肿大,高峰期周产蛋率仅为72%,明显低于同场区未发病鸡群的83%,更显著低于其标准产蛋率（87%）。利用RT-PCR技术对肿大的脏器分别进行分子病原学诊断,禽网状内皮增生症病毒、戊型肝炎病毒和马立克氏病毒等PCR检测结果阴性,而ALV pol基因检测阳性,经测序与GenBank 已发表的ALV E 亚群毒株的核苷酸同源性高达97.5%-99.5%。血清学检测证实, ALV-AB亚群在发病前阳性率为8.3%,而发病后的抗体阳性率则攀升为70%。鸡群发病后的种蛋蛋清p27抗原检测ALV阳性率为5.3%。综上所述,鸡群可能混合感染ALV。 相似文献
19.
于2017～2019年在我国6个省份的番茄主产区，采集疑似番茄褪绿病毒（tomato chlorosis virus，ToCV）和（或）番茄黄化曲叶病毒（tomato yellow leaf curl virus，TYLCV）侵染的番茄样本，利用特异性引物进行RT-PCR 或PCR 扩增，将符合预期条带大小的PCR 产物测序后进行系统发育分析。结果表明，我国6 个省份番茄ToCV 和TYLCV 的复合侵染率为25.29%，其中山东省两种病毒复合侵染率最高，为46.43%。序列分析结果表明，测定的ToCV CP 基因序列与已报道的对应序列同源性在98.30% 以上，ToCV CP 基因序列没有发生明显的遗传分化；TYLCV 基因组部分序列与已报道的对应序列同源性在96.00% 以上，没有发生明显的遗传分化。 相似文献
20.
猪流感病毒与繁殖与呼吸综合征病毒、猪瘟病毒、圆环病毒、伪狂犬病病毒、副猪嗜血杆菌混合感染的情况调查 总被引：3，自引：1，他引：2
应用套式PCR方法对采自广西境内13个市122个不同规模猪场及农村散养户的126份病料,进行了SIV的检测,并对鉴定为SIV阳性的15份病料,再分别进行PRRSV、CSFV、PCV-2、PRV、HPS的检测,以调查广西猪群中SIV与PRRSV、CSFV、PCV-2、PRV、HPS混合感染的情况。结果发现：猪群中SIV感染率为11.9%(15/126),而在所检测的15份阳性病料中,SIV混合感染十分严重,感染率为73.3%(11/15)。混合感染病毒种类最多达四重感染（SIV+PRRSV+CSFV+PCV-2）,占6.67%(1/15);三重感染(SIV+PRRSV+PCV 2、SIV+PRRSV+CSFV) 占40%(6/15);二重感染（SIV+PRRSV,SIV+PCV-2）占26.6%(4/15)。调查结果表明广西发病猪群中SIV与PRRSV、CSFV、PCV-2混合感染普遍存在。 相似文献