Molecular detection of BHV-1 in artificially inoculated semen and in the semen of a latently infected bull treated with dexamethasone     

Grom J  Hostnik P  Toplak I  Barlic-Maganja D 《Veterinary journal (London, England : 1997)》2006,171(3):539-544

Two polymerase chain reaction (PCR) assays specific for glycoprotein B (gB) and glycoprotein E (gE) gene detection, respectively, were adopted for the detection of bovine herpesvirus-1 (BHV-1) in naturally infected bulls. The methods were tested on bovine semen artificially inoculated with BHV-1 and were compared with an optimised virus isolation method. Raw and extended semen samples were diluted in minimal essential medium (MEM) and spiked with equal dose of BHV-1. The extended semen was found to be more toxic for the cells than the raw semen, while the viral DNA could be detected by the PCR method in all tested dilutions of raw and extended semen samples. The sensitivity of both methods was compared also for BHV-1 detection in semen, nasal swabs and leucocytes of a seropositive bull in a different time period after virus reactivation with dexamethasone treatment. The sensitivity of virus detection by the PCR method was equivalent to that of virus isolation in cell culture. However, PCR was shown to be faster and easier to perform and may be a good alternative to virus isolation especially when bovine semen has to be screened for BHV-1 prior to artificial insemination.  相似文献   

陕西2009年初冬区域性暴雪诊断和中尺度分析     

胡皓  梅笑冬  许新田  张潇予 《安徽农业科学》2012,(30):14879-14882,14968

利用实况观测资料、NCEP 1°×1°6 h再分析数据,对2009年11月9～11日陕西近20年最大区域性暴雪过程进行了诊断分析,讨论天气尺度及中尺度系统在促进暴雪形成中的作用。结果表明,此次暴雪是由高空西风槽、中低层切变线、西南急流及地面倒槽、地形共同影响所致。700 hPa切变线使南下冷空气与西南急流在陕西交汇,且700、850 hPa分别有西南急流和偏东急流带来充沛水汽;地面有东北冷空气侵入,既对暖空气起抬升作用,同时配合陕西地形作用加强了地面中尺度辐合带的形成,为暴雪产生提供了有力的动力机制条件。  相似文献   

鸡新城疫的诊断与防治措施     

谢梅 《吉林农业科学》2012,37(4):45-47

鸡新城疫(newcastle disease,ND)是由新城疫病毒(newcastle disease virus,NDV)引起的一种急性、热性、败血性和高度接触性传染病,具有很高的发病率和病死率,是危害养禽业的一种主要传染病。本文将就ND的诊断和防治措施做一详细的综述。  相似文献   

家兔呼吸道传染病主要病因、鉴别诊断及防控     

鲍国连  刘燕  韦强  肖琛闻  季权安 《中国养兔杂志》2012,(3):29-31

兔呼吸道传染病是由多种病原菌单独或混合感染引起,近年来呈现上升的趋势。针对我国家兔呼吸道病高发现状,就呼吸道病的主要病因、主要病原鉴别诊断及防控技术进行论述。  相似文献   

卵黄抗体及其在重大动物疫病防治与诊断中的应用进展     

江馗语  赵刚 《现代畜牧兽医》2013,(10)

卵黄抗体（Immunoglobulin of yolk,IgY）又称卵黄免疫球蛋白,具有产量高、成本低、利于生产、产生有效免疫反应所需抗原量小、免疫检测时有较高的敏感性、无副作用、性质稳定等优点,近年来对其研究越来越多,认识也越来越深入。本文就卵黄抗体的产生、结构、特点及其在猪瘟、猪蓝耳病、禽流感和口蹄疫等重大动物疫病防治与诊断中的应用进展作以综述。  相似文献   

Microbial and nutritional aspects of feeding fermented feed (poultry by‐products) to pigs     

H.A.P. Urlings  A.J. Mug  A.Th. van ‘t Klooster  P.G.H. Bijker  J.G. van Logtestijn  L.G.M. van Gils 《The Veterinary quarterly》2013,33(4):146-151

Summary

Broiler by‐products (heads, feet, and viscera) mixed with 4% dextrose were pasteurized for 4 min at 90°C core temperature, cooled to 20°C, and fermented with Lactobacillus plantarum as starter culture. These fermented poultry by‐products were fed to 12 individually housed fattening pigs as part (17.6% of the dry matter) of their fattening ration, the remainder composed of compound pig feed. Control pigs received a compound pig feed only. Both groups of pigs were fed restrictively on the basis of body weight. The technical results of the pigs fed the experimental diet showed a significantly improved feed:gain ratio (2.46 vs 2.57), a significantly higher carcass weight (86.1 vs 81.8 kg), a lower meat percentage (50.9 vs 52.5%) and an increased backfat thickness (21.5 vs 18.7%). The bacterial flora in the intestinal tract of the pigs fed the experimental diet differed significantly from the control animals. Decreased colony counts of mesophilic aerobic bacteria, Enterobacteriaceae, enterococci and lactobacilli were found in the rectal content and the prevalence of salmonella was lower.

It is suggested that the improved feed:gain ratio and the reduced bacterial activity of the measured groups of bacteria is a result of 1) the higher energy content of the diet, and(or) 2) an assumed enhanced digestibility of nutritional components in the diet, and(or) 3) the lower incidence of diarrhea in the pigs fed with fermented poultry by‐products. This resulted in a lower contamination level of enteropathogenic bacteria like, salmonella and Escherichia coli, in the gastro‐intestinal tract of the pigs fed fermented poultry by‐products.  相似文献   

Plasma‐Free Metanephrine and Free Normetanephrine Measurement for the Diagnosis of Pheochromocytoma in Dogs     

R. Gostelow  N. Bridger  H.M. Syme 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2013,27(1):83-90

Background

Measurement of plasma‐free metanephrines is the test of choice to identify pheochromocytoma in human patients.

Objectives

To establish the sensitivity and specificity of plasma‐free metanephrine (fMN) and free normetanephrine (fNMN) concentrations to diagnose pheochromocytoma in dogs.

Animals

Forty‐five client‐owned dogs (8 dogs with pheochromocytoma, 11 dogs with adrenocortical tumors, 15 dogs with nonadrenal disease, and 11 healthy dogs.)

Methods

A prospective study. EDTA plasma was collected from diseased and healthy dogs and submitted for fMN and fNMN measurement by liquid chromatography‐tandem mass spectrometry (LC‐MS/MS).

Results

Free MN concentration (median [range]) was significantly higher in dogs with pheochromocytoma (8.15 [1.73–175.23] nmol/L) than in healthy dogs (0.95 [0.68–3.08] nmol/L; P < .01) and dogs with adrenocortical tumors (0.92 [0.25–2.51] nmol/L; P < .001), but was not different from dogs with nonadrenal disease (1.91 [0.41–6.57] nmol/L; P ≥ .05). Free NMN concentration was significantly higher in dogs with pheochromocytoma (63.89 [10.19–190.31] nmol/L) than in healthy dogs (2.54 [1.59–4.17] nmol/L; P < .001), dogs with nonadrenal disease (3.30 [1.30–10.10] nmol/L; P < .001), and dogs with adrenocortical tumors (2.96 [1.92–5.01] nmol/L); P < 0.01). When used to diagnose pheochromocytoma, a fMN concentration of 4.18 nmol/L had a sensitivity of 62.5% and specificity of 97.3%, and a fNMN concentration of 5.52 nmol/L had a sensitivity of 100% and specificity of 97.6%.

Conclusions and Clinical Importance

Plasma fNMN concentration has excellent sensitivity and specificity for the diagnosis of pheochromocytoma in dogs, whereas fMN concentration has moderate sensitivity and excellent specificity. Measurement of plasma‐free metanephrines provides an effective, noninvasive, means of identifying dogs with pheochromocytoma.  相似文献   

Urinary Biomarkers for Acute Kidney Injury in Dogs     

J. De Loor  S. Daminet  P. Smets  E. Meyer 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2013,27(5):998-1010

Routinely, kidney dysfunction and decreased glomerular filtration rate (GFR) are diagnosed by the evaluation of changes in the serum creatinine (SCr) and blood urea nitrogen (BUN) concentrations. However, neither of these tests is sensitive or specific enough for the early diagnosis of impaired kidney function because they are both affected by other renal and nonrenal factors. Furthermore, kidney injury can be present in the absence of kidney dysfunction. Renal reserve enables normal GFR even when nephrons are damaged. Renal biomarkers, especially those present in urine, may be useful for the study of both acute and chronic nephropathies. The aim of this review is to describe the current status of urinary biomarkers as diagnostic tools for kidney injury in dogs with particular focus on acute kidney injury (AKI). The International Renal Interest Society (IRIS) canine AKI grading system and the implementation of urinary biomarkers in this system also are discussed. The discovery of novel urinary biomarkers has emerged from hypotheses about the pathophysiology of kidney injury, but few proteomic urine screening approaches have been described in dogs. Lack of standardization of biomarker assays further complicates the comparison of novel canine urinary biomarker validation results among studies. Future research should focus on novel biomarkers of renal origin and evaluate promising biomarkers in different clinical conditions. Validation of selected urinary biomarkers in the diagnosis of canine kidney diseases must include dogs with both renal and nonrenal diseases to evaluate their sensitivity, specificity as well as their negative and positive predictive values.  相似文献   

Evaluation of cobas Influenza A/B & RSV Test for Diagnosis of Equine Influenza     

《Journal of Equine Veterinary Science》2020

  相似文献   

骆驼伊氏锥虫病LAMP实验诊断初报   总被引：2，自引：0，他引：2  

杨帆  王振宝  王常汉  巴音查汗 《中国兽医寄生虫病》2008,16(3):23-25

目的为了探索伊氏锥虫病诊断的新方法。方法采用环状介导等温DNA扩增法（LAMP）,分别对随即采样的14峰骆驼进行伊氏锥虫病诊断,并与血液涂片法对比。结果LAMP与血液涂片法检测得出的阳性率分别为35．71％和14．29％。结论LAMP可用来检测骆驼伊氏锥虫病,并且LAMP在等温条件下进行,不需要复杂的仪器,为临床检测锥虫病提供了一个快速简便的分子生物学方法。  相似文献