Improving the specificity of immunodiagnosis for porcine brucellosis     

Thirlwall RE  Commander NJ  Brew SD  Cutler SJ  McGiven JA  Stack JA 《Veterinary research communications》2008,32(3):209-213

This report describes the use of cell mediated immunity to improve specificity of current diagnosis for Brucella suis. Diagnosis is problematic due to cross reactions that lead to false positive serological reactions (FPSR) in the standard diagnostic tests. A common cause of this cross reactivity is infection with the organism Yersinia enterocolitica O:9. Gottingen™ mini-pigs were experimentally infected with B. suis biovar I field strain or Y. enterocolitica serotype O:9 biotype 3. Infection was followed for 70 days. During this time whole blood stimulation assays were set up using Brucella specific antigen. IFNγ was measured in the supernatants (SN) from these assays by ELISA. Concurrent standard serological tests were carried out. The results indicate that the IFNγ assay is specifically able to distinguish Y. enterocolitica O:9 infection from a B. suis infection in experimentally infected mini-pigs. These results represent an improvement in diagnostic specificity compared to currently used serological tests. Thus suggesting that in a surveillance setting this test could be applied as a confirmatory test in the face of FPSR. The authors are British Civil Servants and as such their work is subject to British Crown Copyright. This means the exclusive copyright for the article cannot be transferred.  相似文献   

一例山羊胰阔盘吸虫病的诊治     

李中波  尧国民  罗世明  罗维  胡辉  罗春光  侯强红 《湖南畜牧兽医》2019,(3):32-34

胰阔盘吸虫病主要由胰阔盘吸虫寄生于牛、羊等反刍动物的胰脏及胰管内,引起机体的胰腺坏死或胰管阻塞的一类疾病。动物感染此病后,主要表现出消瘦、贫血、水肿及消化功能障碍等一系列临床症状。在本次诊治过程中,通过临床症状与病理剖解相结合的方式确定该病病原为胰阔盘吸虫。我们采用吡喹酮+氯氰碘柳胺+安乃近+林可霉素等四种药物对羊群进行驱虫,该养殖场的病情得到基本控制。  相似文献   

Inappropriate Urination     

《Veterinary Clinics of North America: Small Animal Practice》2019,49(2):141-155

  相似文献   

奶牛乳房炎诊断技术的研究进展     

张楠楠  张佩华  高明  刘光磊  叶耿坪  王传蓉 《中国奶牛》2019,(3):24-28

奶牛乳房炎是影响奶牛生产的主要疾病之一。病原菌的免疫抑制以及日趋严重的耐药性,致使乳房炎的发病率增高且治愈困难,给世界奶牛养殖业造成了巨大的经济损失。实践表明,疾病的诊断是疾病病因和治疗之间最重要的一步。经济、快速、可靠的诊断工具是乳房健康管理的基础。本文主要论述了奶牛乳房炎不同诊断方法的优缺点以及新兴诊断技术的研究进展。  相似文献   

Comparison of polymerase chain reaction methods for the detection of <Emphasis Type="Italic">Theileria equi</Emphasis> infection using whole blood compared with pre-extracted DNA samples as PCR templates     

Alhassan A  Iseki H  Kim C  Yokoyama N  Igarashi I 《Tropical animal health and production》2007,39(5):369-374

Rapid, efficient, and reproducible procedures for isolating DNA before PCR gene amplification are essential for the diagnosis of piroplasms. In this study, we evaluated the ease and reliability of detecting Theileria equi by PCR using pre-extracted DNA samples (by QIAamp DNA Mini Kit and phenol-chloroform methods) compared with blood spotted on FTA cards as PCR templates. Although minimal variations in limit of detection were observed among the methods compared, overall, the use of pre-extracted DNA samples and blood spotted on FTA cards had comparable detection limits. These results indicate that T. equi infection can be efficiently detected directly from FTA cards by PCR without the need for pre-extraction of DNA from blood samples.  相似文献   

Getting a grip on strangles: recent progress towards improved diagnostics and vaccines   总被引：1，自引：0，他引：1  

Waller AS  Jolley KA 《Veterinary journal (London, England : 1997)》2007,173(3):492-501

'Strangles', caused by infection with the bacterium Streptococcus equi, remains one of the most commonly diagnosed and important infectious diseases of horses world-wide. This review discusses the diagnosis and pathogenesis of strangles with particular attention to the significance of persistent infections in disease transmission and the rapid progress now being made towards the development of effective preventative vaccines. It is now possible combine recent sequence data from the N-terminal region of the SeM protein and reassign the SeM alleles using the on-line database http://pubmlst.org/szooepidemicus/seM/. Hypotheses concerning the origin of this variation and the potential for its exploitation for the epidemiological analysis of outbreaks are proposed. Advances in understanding of the molecular evolution of S. equi highlight the role played by phage-mediated acquisition of virulence factors and suggest new avenues for prophylactic intervention.  相似文献   

Update on the diagnosis of Haemophilus parasuis infection in pigs and novel genotyping methods   总被引：1，自引：0，他引：1  

Olvera A  Segalés J  Aragón V 《Veterinary journal (London, England : 1997)》2007,174(3):522-529

Haemophilus parasuis causes Gl?sser's disease as well as a number of other diseases in pigs. The diagnosis of H. parasuis-associated disease is usually established by clinical signs, pathological findings and bacterial isolation but diagnosis is complicated by the existence of non-virulent strains and the early colonisation of the upper respiratory tract of healthy piglets. Moreover, several strains can be found on a farm and even within a single animal so it is important to determine the specific strain that is causing the clinical outbreak. Recently, genotyping methods have been developed with the goal of correlating genotype with the degree of virulence of H. parasuis strains. The association between genotype and virulence in H. parasuis is challenging due to the lack of knowledge of the complete genomic sequence and virulence factors of this bacterium.  相似文献   

鸡腺胃型传染性支气管炎的调查研究     

张安国  刘彦  孙颐龄  王余  单景龙 《天津农学院学报》1999,(3)

１９９７ 年５ 月以来,天津市部分养鸡场鸡群连续发生一种以腺胃肿大为特征的新型传染病。经流行病学、临床症状、病理剖检、实验室排异诊断、病毒分离、病毒生物学特性、回归试验等一系列研究,确诊该病为鸡腺胃型传染性支气管炎。分离病毒株定名为 Ｊ Ｂ９７０２株。  相似文献   

汽车主动阻尼减振系统诊断检测研究     

蒙留纪  蒙倩娜 《拖拉机与农用运输车》2010,37(3):27-28

在运行与道路试验检测中,若主动阻尼系统发生故障,控制单元可存储并显示故障码,可按代码及症状对控制电路、电器元件实施诊断与检测;若无故障码显示,需多次实施道路试验,使故障显示症兆,研究其症侯,然后对电路及元器件实行诊断检测。  相似文献   

犬尿石症发病原因及现状分析进展     

李阳枫  张垚 《农业科学与技术》2016,(12):2828-2833

犬尿石症是临床上常见的一种泌尿系统疾病,指在犬矿物质代谢紊乱情况下,尿液中析出的盐类结晶以脱落的上皮细胞等异物为核心逐渐形成的矿物质凝结物,积滞于尿路并刺激尿路黏膜引起出血、炎症和阻塞的一种泌尿系统疾病。笔者就犬尿结石症的结石成因、分类、治疗、预防等方面进行了综述。  相似文献