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21.
Wei-Dong Sun Jin-Yong Wang Ke-Chun Zhang Xiao-Long Wang 《Research in veterinary science》2010,88(3):461-466
To learn more about the biomineralization process of struvite in ruminants, a seldom noticeable crystal, struvite-K, was investigated in six goats in which urolithiasis was induced by feeding a cottonseed meal and rice straw diet supplemented with MgO. The compositions of crystals and calculi were studied by chemical qualitative analysis and X-ray energy dispersive spectrometry (EDS). The activity product (AP) and relative supersaturation (RSS) of magnesium ammonium phosphate (MAP) and potassium magnesium phosphate (MKP) were calculated to compare the difference of crystals formed in this process. The results showed that calculi consisted of MAP with a little MKP. Crystals in the urine consisted of MAP and MKP before stone formation, but crystals in urine after stone formation mainly consisted of MKP. The AP and RSS of MAP and MKP significantly decreased after stone formation. It was concluded that MAP and MKP may coexist in the crystals of urine before struvite calculi formation but MKP did not precipitate to struvite calculi and was separated out as crystal sedimentation of urine. The changes of crystallization of MAP and MKP contributed partially to the supersaturation status of MAP and MKP during struvite stone formation. 相似文献
22.
影响产蛋下降综合征H_(91)疫苗株HA效价的因素 总被引：1，自引：0，他引：1
鸡产蛋下降综合征（ＥＤＳ－７６）Ｈ９１－１疫苗株ＨＡ效价受接种种毒的浓度和收获时间、鸡红细胞浓度、ＨＡ反应温度、灭活用甲醛溶液浓度、灭活尿囊液存放时间等因素影响，分析了提高ＥＤＳ－７６Ｈ９１株ＨＡ效价的方法 相似文献
23.
McAb捕获抗原检测EDS76病毒抗体的ELISA方法的建立和应用 总被引：1，自引：0，他引：1
将三株EDS76病毒单克隆抗体IgG混合,包被ELISA板,捕获病毒抗原后用于检测鸡血清中的EDS76抗体,以OD490nm＞0.3,P/N＞2.1判为阳性结果.经测定,待检血清做1100倍稀释时可以很好地区分阴阳性.所建方法与鸡的其它传染病血清无交叉反应,可被免抗EDS76病毒高免血清竞争性抑制.对11个鸡场330份未接种EDS76疫苗的产蛋鸡血清检测结果表明,血清阳性率最高可达93.33%. 相似文献
24.
用高压均质机制备的ＮＤ－ＥＤＳ７６二联油乳剂灭活疫苗,经３０００ｒｐｍ,１０ｍｉｎ离心不破乳,３７℃恒温保存３２ｄ以上未破乳,粘度为３～４Ｓｅｃ;用该油乳剂灭活疫苗接种商品蛋鸡,每羽经肌肉注射０．５ｍＬ,接种１４ｄ后即可产生足够保护力的中和抗体,２个月时间抗体可达高峰;接种５０周后,ＮＤＨＩ抗体效价仍维持在８．２ｌｏｇ２,ＥＤＳ７６ＨＩ抗体效价７．０ｌｏｇ２。在４～８℃冰箱中保存,有效期在一年以上,经８０万头份中间试验证明,该疫苗对外来品种鸡和地方品种鸡同样有效,能有效控制ＮＤ和ＥＤＳ７６两种疾病。 相似文献
25.
研究利用电子探针能谱（ＥＤＳ）检测薄膜厚度的技术。该技术的核心是：①确定特征ｘ射线的临界激发能Ｅｆ;②确定所采用的半经验公式。Ｅｆ方法是一种很好的估测膜厚的近似方法,具有很好的实用价值。文中还探讨了二种确定薄膜Ｅｆ的方法。 相似文献
26.
减蛋综合征油佐剂灭活疫苗的研制 总被引：1，自引：0，他引：1
减蛋综合征（ＥＤＳ）病毒南京毒株（ＮＥ４）在鸭胚中增殖后，用甲醛灭活，加入７号白油，制成ＥＤＳ油佐剂灭活疫苗，抗原量为每头份５０００血凝（ＨＡ）单位。经１２００万只鸡的试用表明，该苗能够有效地预防ＥＤＳ的发生。 相似文献
27.
分别用三个滴度的ＮＤＶ、ＥＤＳ_７６Ｖ、ＩＢＶ作正交配比，制成八组不同配比的三联油佐剂灭活苗，鸡免疫后每月采血分离血清，应用ＨＩ监测其抗体，结果表明，三种病毒适当配比，不产生干扰，疫苗的免疫效果与抗原量有关。本疫苗与Ｉｎｔeｒｖｃｔ公司生产的ＮＤ－ＩＢ－ＩＢＤ三联油佐剂灭活苗作比较试验，免疫鸡所产生的ＮＤ、ＩＢ抗体无显著性差异。应用含抗原量最低口比的疫苗，对不明原因的有产蛋率下降疫病感染史的鸡群作免疫接种，冬季该场蛋鸡群普遍发生产蛋率明显下降的疫病，用本苗免疫的鸡群产蛋享未受影响。 相似文献
28.
ABSTRACTUltrafine fish bone powder (UFBP) from silver carp (Hypophthalmichthys moli trix) was prepared using dry media milling. The characteristics of UFBP were investigated by a laser particle size analyzer, Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), energy dispersive X-ray spectroscopy (EDS), and inductively coupled plasma (ICP) spectrometry. The size and specific surface area of UFBP particles were significantly (p < 0.05) affected by rotation speed, media/powder weight ratio, media diameter, and milling time. With the following conditions: rotation speed of 400 revolutions per minute, ratio of media to fish bone powder at 4:1, media diameter of 2 mm and 2.5 h of milling time, the optimal values of particle size (1.75 μm) and specific surface area (7.75 m2/g) were obtained. The SEM results confirmed that the particle became smaller and more uniform after milling treatment. Ash (63.71%) and protein (20.52%) were the main components of UFBP, and total calcium content was determined to be 236.90 mg/g in dried UFBP. As UFBP particle size decreased, calcium solubility significantly increased (p < 0.05), calcium content on the surface layer decreased, but the chemical compositions and structure did not change. 相似文献
29.
Scanning Electron Microscope and Energy Dispersive Analysis (SEM EDS) were used to study the elements of particles in drinking water treatment effluents, to conduct the distribution characteristics of particles and distribution probability of main elements, and to analyse the possible structure of individual element in the Granular Activated Carbon(GAC) effluents particles. It was indicated that there were more elements in particles at GAC inlet and outlet, in which the major elements were Ca、Fe、Al 、Si 、C、O. And the concentration of non metal elements was absolutely higher than that of metal elements. The shapes of particles were various, in which most of them were irregular and very few were baculiform, spherical or flocculent. The molar ratio of (C+O) to (Ca+Fe+Al+Mg) in particles had a wide range, from 1.0 to 40, in eight samples, in which ratios of two samples were 1.0-3.0 and five were over 7.0. 相似文献
30.
前期研究表明,茄子 Enhanced disease susceptibility 1（SmEDS1）正调控茄子青枯病抗性,但是其抗病机理有待进一步研究。为筛选 SmEDS1 的互作蛋白,本研究构建 pGBK7-SmEDS1 诱饵载体,并利用酵母双杂交技术对相应的均一化 cDNA 酵母文库进行筛选。从 2 次独立的实验中筛选到 9 个克隆,其非冗余地编码 6 种蛋白,即 TCP 转录因子、DNAJ 分子伴侣、SnRK1 蛋白 α 亚基、氰酸酯酶、CIP4 蛋白和 1 个未知蛋白。基于这些蛋白的功能,推测 SmEDS1 可能通过与本研究筛选到 TCP 转录因子互作调控水杨酸的合成,进而调控茄子的青枯病抗性。 相似文献