鲤鱼源粪肠球菌的体外抑菌试验研究     

单晓枫  钱爱东 《中国兽药杂志》2008,42(6):4-7

为证明益生菌候选菌株具有益生特性,将筛选的鲤鱼源粪肠球菌YL-1进行体外抑菌试验。结果发现,YL-1对病原菌嗜水气单胞菌有较强的抑菌作用;通过对YL-1上清液进行热处理,蛋白酶处理,中和酸等方式分析发现,其抑菌物质主要是对有机酸和胰蛋白酶敏感的一类物质。  相似文献   

乳酸菌对Caco-2细胞分泌APRIL和IL-10的影响     

黄怡  黄琴  李雅丽  崔志文  李卫芬  余东游 《中国农业科学》2012,45(9):1881-1886

【目的】通过检测促炎细胞因子APRIL和抗炎细胞因子IL-10的分泌水平来观察屎肠球菌和乳酸乳球菌对肠上皮细胞先天性免疫应答的调节作用。【方法】Caco-2细胞分别和PBS（CT组,阴性对照组）、Escherichia coli K88（EC组,阳性对照组）,Enterococcus faecium（EF组）或Lactococcus lactis（LL组）共孵育2 h,以及先分别和Enterococcus faecium或Lactococcus lactis共孵育1 h,再和Escherichia coli K88共孵育2 h（EF-EC组和LL-EC组）。试验结束时,用ELISA方法检测细胞培养上清中APRIL和IL-10的含量。【结果】结果表明,2株乳酸菌都促进正常状态下的肠上皮细胞分泌促炎细胞因子APRIL和抗炎细胞因子IL-10,抑制Escherichia coli K88对APRIL分泌的诱导作用,促进Escherichia coli K88感染的肠上皮细胞分泌IL-10。【结论】体外试验条件下,屎肠球菌和乳酸乳球菌能够诱导肠上皮细胞产生先天免疫应答,分泌APRIL和IL-10,抑制大肠杆菌K88引起的促炎反应,表现出抗炎作用。  相似文献   

Classification and Antimicrobial Susceptibilities of Enterococcus Species Isolated from Apparently Healthy Food‐Producing Animals in Japan     

A. Kojima  A. Morioka  M. Kijima  K. Ishihara  T. Asai  T. Fujisawa  Y. Tamura  T. Takahashi 《Zoonoses and public health》2010,57(2):137-141

Summary A total of 1024 enterococci were recovered from faeces of healthy animals from 178 cattle, 178 pig and 156 broiler farms. Enterococcus faecium was the predominant species recovered (35.8%), followed by E. faecalis (31.3%) and E. hirae (25.6%). Oxytetracycline resistance was most frequently found among E. faecalis (85.9%), E. faecium (58.8%) and E. hirae (48.1%). Resistance rates to almost all antimicrobials were higher in E. faecalis than E. faecium and E. hirae. Isolates from cattle were more susceptible to the antimicrobials studied than those from pigs and broilers. VanA‐ or VanB‐type vancomycin‐resistant enterococci have not been found since the ban of avoparcin use 5 years ago.  相似文献   

新疆动物源粪肠球菌的耐药性分析与耐药基因检测     

陈万昭  陈月月  易海波  秦蕾  徐琦琦  吴慧敏  夏利宁 《中国农业大学学报》2022,27(2):110-119

为了解新疆不同动物源粪肠球菌的耐药性和耐药基因携带情况,并指导临床针对不同动物合理用药,从新疆9个县区养殖场分别采集猪、牛、羊、骆驼、鸡和鸽的粪样共564份,进行粪肠球菌的分离鉴定.对分离得到的粪肠球菌采用CLSI推荐的琼脂稀释法进行11种抗菌药物最小抑菌浓度的测定,用PCR方法进行10种耐药基因的检测.结果显示:1)...  相似文献   

The Influence of Shock Treatment on Survival Rate of Intestinal Probiotics during Freezing     

LI Chun ZHANG Lanwei LIU Libo FENG Zhen 《东北农业大学学报(英文版)》2006,13(1):36-40

Probiotics which can provide potential health benefits for consumers and prevent disease, is the most important research field for functional food in the future. It is the key point that how much the count of bacteria and how long it can preserve. Freeze-drying skill is one of the best preservation methods, but it's defect that damage to biological systems can be attributed to changes in the physical state of membrane lipids or/and changes in the structure of sensitive proteins and the decreased survival rate. This work used pretreatment methods-cold shock and hot shock to reduce the damage to bacteria during freeze-drying. Using unpretreated samples as control, aspects of cold shock or heat shock. The response of Enterococcous faecalis A31 was investigated during lag phase, the middle of exponential growth phase and the terminal of exponential growth phase. The results suggested that when cold shock, the shock protein concentration produced by treated sample within 2 h during the middle of exponential growth phase was higher than 4, 8, 24 h, The concentration of shock protein produced by treated sample at 10 ℃ was higher than 20℃, The concentration of sample protein at the middle of exponential growth phase was higher than that at the terminal of exponential growth phase. After one-month storage, the survival rate at 10℃ was better than 20 ℃ and 4 ℃ comparatively. The survival rate at (6 h) 4 ℃/24 h (lag period) was higher than (10 h) 4 ℃/24 h (the middle of exponential growth phase), and the effect of 10 ℃ /8 h shock treatments was best during the middle of exponential growth phase, when hot shock, The concentration of heat shock proteins produced was not obvious, and survival rate was higher at 45℃, 30 min than at 45 ℃, 60 min.  相似文献   

粪肠球菌对吉富罗非鱼的生长、体组成、消化酶活性及血液生理生化指标的影响     

黄旺  李洪琴  罗莉  陈拥军  白富瑾  林肯 《水产学报》2017,41(11):1756-1765

为研究饲料中添加不同浓度的粪肠球菌对吉富罗非鱼生长、体组成、消化能力及血液生理生化指标的影响,实验选用初始体质量为(50.59±0.59)g的吉富罗非鱼300尾,随机分成5组,每组设3个重复,每个重复20尾鱼,养殖实验期60 d。分别投喂实测含1.3×10~2(对照组)、1.4×10~5、1.7×10~6、1.5×10~7和1.8×10~8 CFU/g粪肠球菌的5种等氮(36%)等脂(6.75%)的实验饲料。结果显示:(1)罗非鱼的末均重(FBW)、增重率(WGR)、特定生长率(SGR)和尾均摄食量(FI)均在1.5×10~7 CFU/g组达到最大且显著高于对照组,而饲料系数(FCR)显著低于对照组。以FBW、WGR和SGR为评价指标,通过二次回归分析得出,罗非鱼饲料的粪肠球菌适宜添加浓度范围为7.5×10~7~1.1×10~8 CFU/g。(2)各添加组的全鱼粗蛋白含量均显著高于对照组,1.5×10~7 CFU/g组的粗脂肪含量显著高于对照组,各组间全鱼水分和粗灰分含量无显著差异。1.5×10~7 CFU/g组的干物质、蛋白质和脂肪沉积率均达到最大值且显著高于对照组。(3)1.5×10~7和1.8×10~8 CFU/g组的肠脂肪酶活性均显著高于对照组,而1.8×10~8 CFU/g组的肠道蛋白酶活性显著低于对照组;各组间肠道淀粉酶活性无显著差异。(4)1.5×10~7 CFU/g组的平均红细胞体积、血红蛋白浓度和血小板数均显著低于对照组,各组间红细胞数无显著差异。(5)1.8×10~8 CFU/g组的血清中胆固醇、葡萄糖和丙二醛含量显著低于对照组;1.5×10~7 CFU/g组血清中碱性磷酸酶活性显著高于对照组,而谷丙转氨酶和谷草转氨酶活性显著低于对照组。综上所述,吉富罗非鱼(50~210 g)饲料中粪肠球菌的适宜添加浓度范围为7.5×10~7~1.1×10~8 CFU/g,但添加粪肠球菌对血液载氧能力有负面影响。  相似文献   

Dietary supplement of Enterococcus faecalis on digestive enzyme activities,short‐chain fatty acid production,immune system response and disease resistance of Javanese carp (Puntius gonionotus,Bleeker 1850)          下载免费PDF全文

S. K. Allameh  E. Ringø  F. M. Yusoff  H. M. Daud  A. Ideris 《Aquaculture Nutrition》2017,23(2):331-338

This study investigated the effects of Enterococcus faecalis on digestive enzyme activities and short‐chain fatty acid production in fish intestine, resistance against Aeromonas hydrophila and humoral immunity response by 3 experiments on Javanes carp (Puntius gonionotus). The experiment 1 revealed that diet supplemented with E. faecalis significantly (P < 0.05) increased protease and lipase activities compared to control fed fish. Moreover, E. faecalis supplementation significantly enhanced the production of propionic and butyric acid in the intestine, while no significant difference (P > 0.05) in acetic acid production was observed. In the challenge study (experiment 2), fish were injected (intraperitoneal) with 10⁷ A. hydrophila per ml and survival was significantly improved when fish were fed diet supplemented with E. faecalis compared to control fish. In experiment 3, dietary E. faecalis affected immune system response as fish fed the probiont and exposed to 10⁶ A. hydrophila per ml displayed significantly elevated antibody levels compared to control fed fish. Fish fed diet supplemented with E. faecalis but not exposed to the pathogen revealed significantly higher antibody level than control fish (P < 0.05). Therefore, E. faecalis can be used as a probiotic in Javanese carp farming.  相似文献   

水稻根分泌物及其与粪产碱菌的相互作用   总被引：9，自引：0，他引：9  

林敏  尤崇杓 《中国农业科学》1989,22(6):6-12

水稻植株通过根系向根际分泌多种有机酸、糖、氨基酸和植物激素。平均每克鲜根分泌有机酸2.25mg,柠檬酸含量较高,其次是苹果酸、琥珀酸、乳酸;氨基酸2.61mg,大约有15种,碱性氨基酸含量最高;糖12.6mg,以蔗糖、葡萄糖、果糖为主;植物激素仅测到赤霉酸（GA#-3）。不同水稻品种的根分泌物在组成及含量上都有差异。粪产碱菌在纯培养条件下能分泌9种氨基酸,一种有机酸即苹果酸以及植物激素如生长素（IAA）、赤霉酸（GA#-3）,但不分泌糖。接种粪产碱菌能刺激水稻根的分泌作用,并对根分泌物的组成和含量有所影响。2.63%的水稻光合产物以根分泌物形式释放到根区,接种粪产碱菌后分泌量增至3.52%,并且大部分为粪产碱菌吸收利用。水稻的光合作用及根分泌能力影响根际固氮活性。具有较强分泌能力的植株,其根际固氮活性也较高。  相似文献   

粪肠球菌fsr群体感应系统的研究进展     

赵彬彬  高原 《中国农学通报》2015,31(2):49-54

群体感应系统是微生物间交流和适应周围环境的一种机制。粪肠球菌fsr群体感应系统与粪肠球菌致病性密切相关。综述了粪肠球菌fsr群体感应系统的组成,各部分特点,群体感应系统信号调控通路,fsr群体感应系统相关毒力基因,群体感应系统抑制剂等国内外研究进展,并对粪肠球菌所引起疾病的治疗提出新的建议。  相似文献   

屎肠球菌新序列型ST989菌株的分离鉴定及多重耐药性分析     

张芬芬  李学瑞  杨建设 《中国畜牧兽医》2015,42(5):1274-1282

试验从青海民和县某羊场致病性感染半月龄羔羊中分离出革兰氏阳性菌,感染羔羊临床症状有精神萎靡,采食减少,发病1~2 h后死亡。两只濒死羔羊剖检结果发现,其中一只出现肝脏肿大及肝包膜脱落,其余脏器无明显病理变化;另一只各脏器均未发现病理变化。分离病料,革兰氏染色发现,从病料中分离到的细菌均为革兰氏阳性球菌。对分离菌株进行Tuf基因PCR扩增,BLASTn比对结果显示与屎肠球菌AUS0085核苷酸序列相似性为99.8%,据此初步确定这些菌株为屎肠球菌。药敏试验结果表明,这些菌株可分为两类,第1类对β-内酰胺类、氨基糖苷类、大环内酯类、四环素类、喹诺酮类、利福霉素类抗生素及林可霉素和克林霉素耐药,对氯霉素和万古霉素敏感;第2类对β-内酰胺类、氨基糖苷类、四环素类、喹诺酮类、利福霉素类抗生素耐药,对红霉素和罗红霉素中度耐药,但对克拉霉素、阿奇霉素、克林霉素、林可霉素、氯霉素和万古霉素敏感。对第1类耐药性代表菌株lby1和第2类耐药性代表菌株lbg3进行多位点序列分析(MLST),结果发现这些菌株为一个屎肠球菌的新序列型,为ST989。系统进化树结果显示屎肠球菌lbg3与屎肠球菌ST468亲缘关系最近,致病力试验结果表明屎肠球菌lby1和lbg3均具有致病性。  相似文献