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41.
为对发病鹅感染致病性大肠埃希菌的临床治疗提供指导并防控致病性大肠埃希菌感染,本研究无菌采集腹泻鹅及病死鹅脾脏、肝脏等组织器官进行病原菌分离培养、染色观察、生化鉴定、致病性试验,对菌株部分基因进行测序、构建遗传进化树并进行药敏试验。结果显示,普通琼脂培养基上生长出灰白色、圆形、凸起、边缘整齐菌落,麦康凯琼脂培养基上长出玫红色、光滑圆润菌落;分离菌株经革兰氏染色,镜下可见革兰氏阴性菌,两端钝圆、大小中等,多呈单个存在的杆状菌;致病性试验表明,该分离菌株对小鼠、雏鹅均有较高致死率;遗传进化树结果显示,分离菌株与患者粪便分离株(GenBank登录号:CP024992.1)同源性最高,达99.5%;体外抑菌试验发现菌株耐药情况较严重,对大多数抗菌药均有较强抗性,仅头孢噻呋对该分离株有较强的抑菌作用,合理用药后病情得到有效控制。本研究为有效防治鹅大肠埃希菌病提供了理论依据,对规模化鹅场防控大肠埃希菌等其他细菌性疾病具有重要价值。 相似文献
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XIE Quan-xi ZHANG Jian-mei YU Jia-min QI Xiu-ye XU Hui XU Hai-yan GU Wei 《中国畜牧兽医》2016,43(6):1523-1529
This experiment was designed to study the complex of Lactobacillus rhamnosus fermented herbal and Bacillus subtilis on White Feather broiler immunity performance and impact of Escherichia coli infection.360 one-day-old broiler chickens were randomly divided into 3 groups with 4 replicates in each group and 30 chickens per replicate.The pretrial period lasted for 7 d,and the experiment lasted for 35 d.The chickens in the group Ⅰ(positive control group) and group Ⅱ(negative control group)were all only fed a basal diet,group Ⅲ was test group,by additive 1% fermented herbal preparations,groups Ⅱ and Ⅲ were intraperitoneal injection of 1 mL E.coli at 35 d,broiler mortality,immune organ index,cecalmicroflora content,immunoglobulin levels,IL-2 and IL-6 content were tested.The results showed that injection of E.coli caused massive death of chickens,group Ⅱtook up to 75.00%,it was significantly higher than groups Ⅰ and Ⅲ (P < 0.05),the mortality in group Ⅲ was significantly lower than group Ⅱ(P < 0.05),was only 23.33%.Injection of E.coli maked spleen index and thymus index of group Ⅱincreased significantly (P < 0.05),the spleen index and thymus index of groups Ⅰ and Ⅲ were no significant difference (P > 0.05).E.coli counts was significantly decreased after injectionin group Ⅲ (P < 0.05),but the number of intestinal Lactobacilli of group Ⅲ was significantly increased (P < 0.05),and inhibited the propagation of E.coli,the counts of E.coli in groups Ⅰ and Ⅲ were no significant difference (P > 0.05).At 42 d,the sIgA of the intestinal fluid in group Ⅲ were higher than that of groups Ⅰ and Ⅱ with 11.99% and 36.56%,respectively(P < 0.05).The serum IgG concentrations of group Ⅲ was higher than that of groupsⅠand Ⅱwith 14.68% and 28.15%,respectively(P < 0.05).At 42 d,the IL-2 content of group Ⅱ was the lowest,it was significantly lower than group Ⅲ(P < 0.05),the IL-6 of group Ⅲ was significantly lower than group Ⅱ(P < 0.05). 相似文献
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YAO Wei YU Xue-wu CAO Dong ZHAO Xiao-tong GU Gui-bo CHEN Yao ZHANG Lei LIU Yao-chuan FENG Jun-ke 《中国畜牧兽医》2016,43(11):3080-3084
In order to understand the main bacterial pathogen species causing dairy cow mastitis in Liaoning, as well as the characteristics of drug sensitivity of the pathogenic E.coli,the milk samples from 75 dairy cows with clinical manifestations for mastitis in certain large-scale dairy farm in Liaoning were collected.The bacteria in milk were cultured and isolated with biochemical methods and in vitro drug sensitivity tests were processed with the isolated E.coli strains.The results showed that the main bacterial pathogen for dairy cow mastitis were E.coli(separation rate 58.7%),S.aureus(64.0%)and S.agalactiae(54.7%),and multiple infection including double and triple infection were identified.The drug sensitivity tests on the isolated E.coli indicated that the E.coli isolates were highly resistant to sulfonamides(resistance rate>85%)and chloramphenicol(resistance rate>30%),and they were relatively low resistant to ampicillin(9.5%),ciprofloxacin(9.5%),ceftiofur(7.1%)and ofloxacin(4.8%).The results was able to provide reliable theoretical basis for prevention and control of dairy cow mastitis in Liaoning area. 相似文献
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为了研究噬菌体尾丝蛋白gp37在识别宿主菌大肠埃希菌K12过程中的作用,PCR扩增获得gp37基因C端1 161bp序列,以EGFP为荧光标签,构建重组表达质粒pET-28a-EGFP-gp37,在大肠埃希菌BL21(DE3)中IPTG诱导表达重组蛋白EGFP-gp37,亲和层析纯化蛋白。SDS-PAGE及Western blot检测结果表明,重组蛋白EGFP-gp37在大肠埃希菌BL21(DE3)为可溶性表达,表达量占菌体总蛋白量的23%。EGFP-gp37融合蛋白与大肠埃希菌K12相互作用,激光共聚焦显微镜检测结果显示,尾丝蛋白gp37能够吸附到宿主菌K12表面,表明尾丝蛋白gp37参与宿主菌受体的识别和吸附过程。 相似文献
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广东水禽源大肠埃希菌耐药性及氨基糖苷类耐药基因研究 总被引:1,自引:0,他引:1
为了调查广东地区水禽源大肠埃希菌对氨基糖苷类药物耐药现状和耐药基因的流行情况,探索大肠埃希菌的氨基糖苷类耐药基因型与耐药表型之间的关系,本研究采用琼脂梯度稀释法测定251株广东地区水禽源大肠埃希菌对氨基糖苷类药物的耐药性和采用PCR方法检测耐药基因。药敏试验结果显示,大肠埃希菌对链霉素耐药较严重,鸭源和鹅源的耐药率分别为81.58%和75.43%,对庆大霉素和卡那霉素较敏感,耐药率分别为25%和54.86%,对阿米卡星最为敏感,其中鸭源的菌株耐药率仅为1.32%。PCR方法检测显示,aadA1和aph(3′)-1检出率较高,为84.6%和91.9%,表明携带aadA1和aph(3′)-1耐药基因的水禽源大肠埃希菌在广东地区呈流行趋势。耐药基因型与耐药表型相关性分析结果显示,耐药基因rmtB的携带与4种药物(庆大霉素、阿米卡星、卡那霉素和壮观霉素)耐药株的产生具有显著相关性,表明耐药基因rmtB对大肠埃希菌耐药株的产生起重要的作用。本试验结果可为指导广东地区水禽大肠埃希菌病的临床用药提供理论基础和研究大肠埃希菌的耐药基因提供相关的数据。 相似文献
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Yeon Soo Chung Jae Won Song Dae Ho Kim Sook Shin Young Kyung Park Soo Jin Yang Suk Kyung Lim Kun Taek Park Yong Ho Park 《Journal of veterinary science (Suw?n-si, Korea)》2016,17(2):199-206
Limited information is available regarding horse-associated antimicrobial resistant (AR) Escherichia (E.) coli. This study was designed to evaluate the frequency and characterize the pattern of AR E. coli from healthy horse-associated samples. A total of 143 E. coli (4.6%) were isolated from 3,078 samples collected from three national racetracks and 14 private horse-riding courses in Korea. Thirty of the E. coli isolates (21%) showed antimicrobial resistance to at least one antimicrobial agent, and four of the AR E. coli (13.3%) were defined as multi-drug resistance. Most of the AR E. coli harbored AR genes corresponding to their antimicrobial resistance phenotypes. Four of the AR E. coli carried class 1 integrase gene (intI1), a gene associated with multi-drug resistance. Pulsed-field gel electrophoretic analysis showed no genetic relatedness among AR E. coli isolated from different facilities; however, cross-transmissions between horses or horses and environments were detected in two facilities. Although cross-transmission of AR E. coli in horses and their environments was generally low, our study suggests a risk of transmission of AR bacteria between horses and humans. Further studies are needed to evaluate the risk of possible transmission of horse-associated AR bacteria to human communities through horse riders and horse-care workers. 相似文献
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