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91.
把 2 0只健康家兔随机分成 4组 ,第 组为对照组 ,正常饲喂 ,第 、 及 组作为中毒试验组 ,分别经口灌服 1 .5mg/ kg、2 .0 mg/ kg、1 0 .0 mg/ kg体重的氟乙酰胺溶液 ( 56 .8mg/m L) ;各中毒组家兔死亡前从心脏采取血样 ,未死亡家兔和对照组家兔在实验第 3天从心脏采取血样 ,测定血清中 AST、AL T、CK、L DH活力和 CRE、Urea含量。结果显示 :血清中 AST、AL T、CK、L DH活力明显升高 ( P <0 .0 5或 P<0 .0 1 ) ; 组家兔血清 Urea含量显著升高 ( P<0 .0 5) , 、 组与对照组差异极显著 ( P <0 .0 1 ) ;各中毒组家兔血清 CRE含量也有不同程度升高 ,但只有 组与对照组有显著性差异 ( P<0 .0 5)。实验说明家兔氟乙酰胺中毒后心脏、肝脏、肾脏等组织器官受到损伤 相似文献
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A sensitive gel-diffusion assay for determination of phospholipase A2 was developed. PLA2 standards, serum, faecal and pancreas homogenate samples with PLA2-activity were allowed to diffuse from wells into agar-gels containing lecithin-membranes. The turbidity cleared radially upon PLA2-activity. The diameters of the cleared zones showed a linear relationship with the log of the enzyme concentration. Serum samples resulted in some turbidity within the cleared zones. This interference originating from serum lipoproteins could be abolished by hydrophobic absorption. The gel-diffusion method was compared with two other methods for PLA2, titrimetric and radiometric techniques. Analysis on 37 human patients with acute pancreatitis showed close interrelationship between these methods. The phospholipase A2 activity in sera from man, the dog, the horse, the cow, the pig and the cat were almost equal, but much less than in the albino rat. No significant differences between PLA2 activities in pancreatic samples were obtained in different animal species. Of the faecal samples, the cow had the lowest PLA2 activity. Dogs suffering from pancreatic degenerative atrophy (PDA), had significantly reduced PLA2 activity both in their pancreas and faeces but not in serum. 相似文献
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三种绿化树种叶片中氟含量的累积 总被引:1,自引:0,他引:1
[目的]筛选对氟污染抗性强的绿化树种。[方法]采集化肥厂周围柳树、松树及榆树的树叶,用氟离子选择电极法测定叶片中氟含量,了解不同树种对氟的富集累积情况。[结果]用高氯酸浸提法提取并用标准曲线回归方程计算的柳树、松树及榆树叶片中氟含量分别为13.38、14.31、15.32 mg/kg,硝酸-氢氧化钠浸提法的柳树、松树及榆树叶片中氟含量分别为11.90、13.28、14.40 mg/kg。试验加标回收率是97%~103%,相对标准偏差是4.19%,表明该方法测定氟含量准确性高,达到质控要求。[结论]3种绿化植物氟的富集能力大小为榆树>松树>柳树。 相似文献
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D.J. Undersander 《Journal of plant nutrition》2013,36(11):977-988
Winter wheat (Triticum aestivum L.) is an important source of forage for cattle in the southern Plains. While high in quality, wheat pasture is prone to cause thousands of cattle deaths annually from nitrate poisoning, bloat, and wheat pasture poisoning, a syndrome of unknown cause. A large portion of the stocker cattle deaths on nitrogen fertilized pastures occur from unknown causes that appear to be related to forage composition. The objectives of this study were to determine seasonal trends in nutrient composition and to determine the effect of nitrapyrin, a nitrification inhibitor, on the change in nutrient composition of wheat forage over winter. The wheat was planted the last week of August on a Pullman clay loam soil (fine, mixed, thermic Torrertic Paleustol). Clip samples were collected periodically and analyzed for nutrient composition. Nitrogen, phosphorus, and potassium concentrations of the wheat forage varied during the season. Levels tended to be highest with the onset of growth and to decline as growth was decreased by cold weather. Nitrapyrin did not affect the concentration of any of the nutrients examined in the wheat forage. Concentration of nutrients, other than potassium, tended to be highly correlated with each other in both years of the study. 相似文献