六氟化硫（SF6）示踪法测定反刍动物甲烷排放的技术   总被引：5，自引：0，他引：5  

董红敏  李玉娥 《中国农业气象》1996,17(4):44-46

详细描述了用六氟化硫示踪法测定反刍动物甲烷排放的技术，包括基本原理、仪器准备和测试条件。还研究了适用于中国的采气设备、示踪参数、测定条件等技术，本技术经济实用且易操作，现正在试用。  相似文献   

G418抗性筛选标记在板栗疫病菌中的应用     

蓝秀万陈敏玫  桂磊范云燕  甘文剑陈保善 《广西农业生物科学》2007,26(B06):7-11

板栗疫病菌一低毒病毒系统．是一个优良的研究植物病原真菌致病和病毒一宿主相互作用分子机制的模型。随着研究的深入，原有的苯菌灵和潮霉素两个遗传转化筛选标记已不能满足研究需要。文章报道构建一个由构巢曲trpC启动子驱动的G418抗性Neo基因盒，并成功应用于板栗疫病菌的遗传转化研究。  相似文献   

DA-6对温室桃树光合作用和叶绿体活性的影响   总被引：7，自引：0，他引：7  

单守明  刘国杰  李绍华 《植物营养与肥料学报》2008,14(6):1237-1241

以温室桃树为试材,研究不同浓度的DA-6对桃树叶片光合作用、叶绿体活性和果实品质的影响。结果表明,20 mg/L DA-6处理显著提高了温室桃树叶片的净光合速率、气孔导度和叶绿素含量,还显著提高了叶绿体Hill反应活力,非环式光合磷酸化活性、Rubisco和ATPase活性;而40 mg/L DA-6处理对它们有轻微的抑制作用。所有DA-6处理均提高了果实品质,其中20 mg/L DA-6处理显著提高了果实平均单果重和可溶性固形物含量,降低了可滴定酸含量,使果实品质得到显著提高。试验表明,适当浓度的DA-6处理可提高温室桃树的光合效率和果实品质。  相似文献   

大白菜细胞质雄性不育的分子鉴定及序列分析   总被引：4，自引：2，他引：2  

张德双  张凤兰  王永健  余阳俊  赵岫云  徐家炳  方智远 《华北农学报》2007,22(6):53-59

为了获得3种大白菜细胞质雄性不育系Ogu CMS,Pol CMS,CMS96和保持系间的多态性以及定位大白菜CMS96不育系所属的不育类型,利用设计的atp6,orf222单一和混合引物PCR扩增3组11份同核异质大白菜细胞质雄性不育系和保持系mtDNA。结果表明,atp6引物在大白菜Ogu CMS不育系扩增的200 bp片段为其特异带;orf222引物仅在大白菜Pol CMS和CMS96不育系有扩增产物,但二者有3点完全不同:大白菜Pol CMS不育系扩增产物为675bp,CMS96不育系扩增产物为669 bp,二者相差6个核苷酸,后者定名为大白菜CMS96-orf222。大白菜CMS96-orf222与甘蓝型油菜Nap CMS的nad5c基因和Nap-orf222基因同源性均为99%,E值为0.0;大白菜Pol CMS的675 bp序列具有ORF224开放阅读框,没有保守结构域,而大白菜CMS96的669 bp序列具有ORF222开放阅读框和保守结构域YMF19。另外,atp6和orf222混合引物多重PCR扩增产物存在明显多态性:800 bp为大白菜保持系的差异带型;2 300 bp和1 500 bp为大白菜Pol CMS不育系特异带型;200 bp为大白菜Ogu CMS不育系特异带型;690 bp为大白菜CMS96不育系特异带型。该方法仅用一次PCR反应快速地将3种大白菜细胞质雄性不育系和保持系一次性全部区分开,为大白菜分子育种和常规育种更好地相结合提供了简单、快速、准确和重复性好的方法和手段。  相似文献   

2个桉树无性系微量元素叶片营养诊断初探   总被引：2，自引：0，他引：2  

李淑仪  蓝佩玲  廖新荣  杨国清  简明  徐胜光 《浙江林学院学报》2005,22(1):40-45

为了进一步提高桉树Eucalyptus产量,对桉树施微肥试验区的叶片进行了分析.采用叶片营养诊断临界值法探讨了2个桉树无性系的微量元素营养状况,初步获得了刚果12W5桉E.ABL 12W5和尾叶桉U6E. urophylla U6的部分微量元素营养的诊断指标,并进一步在中试区作诊断评价和检验.结果表明:桉树叶片的钼、锌、铜、锰的质量分数与一般植物的有差异.2个无性系的微量元素叶片营养诊断临界值,刚果12W5桉为硼27.90 mg·kg-1 ,锌45.00 mg·kg-1 ,铜1.08 mg·kg-1 ,铁288.00 mg·kg-1 ;尾叶桉U6为:硼13.50 mg·kg-1 ,锌10.80 mg·kg-1 ,钼0.15 mg·kg-1 ,锰216.00 mg·kg-1 ,铁117.00 mg·kg-1 ,其中桉树叶片养分诊断标准与微肥中试区的叶片分析数据相吻合的有这2种桉树叶片中的硼,尾叶桉中的钼,刚果桉中的铜;而中试区的桉树叶片锌、铁、锰的分析数据均未能与诊断标准相吻合,故这3种元素暂不适宜用临界值法诊断指导施肥.表6参22  相似文献   

植物生长调节剂处理对金桔幼果生长的影响     

陈棕林  周岐伟  崔芸瑜  蒙容君  李东旭  赖家业 《中国果业信息》2023,52(6)

为确定最适用于金桔幼果生长的植物生长调节剂种类及浓度,在金桔第二批花期后幼果膨大期,分别用4种浓度梯度的赤霉素（GA3）、6-苄氨基嘌呤（6-BA）和芸苔素（BR）对金桔果树进行喷施试验,结果表明：各处理果实的横径增加2.47%-12.21%,纵径增加6.33%-16.33%,可溶性蛋白含量增加1.07-1.87倍,过氧化物酶活性提高1.21-5.16倍,有9个处理可溶性糖含量增加,7个处理的还原糖含量提高,9个处理的果形指数增大,各处理对幼果果柄长度无显著增大。对处理后的8个相关品质指标进行主成分分析,结果表明T7(60 mg/L 6-BA)处理更有利于金桔幼果的生长。  相似文献   

6%精喹·嗪酸乳油防除花生田杂草     

刘可平  江海滨  郑志勇 《花生学报》2006,35(4):36-39

田间试验结果表明,6%精喹.嗪酸乳油作茎叶处理对马唐、牛筋草、稗草、反枝苋、藜等杂草均有较好的除草效果。推荐应用剂量为有效量45~54 g/hm2(商品量750~900 mL/hm2),药后20 d对杂草的鲜重防效在95%以上。在推荐剂量范围内对花生安全,一次施药即能有效防除花生田全生育期的杂草,除草效果好。  相似文献   

长沙黑麋峰冬季鸟类资源调查及多样性分析   总被引：3，自引：0，他引：3  

陈顺德  邓学建  李自君  郭克疾 《湖南林业科技》2006,33(5):44-46

通过对黑麋峰森林公园鸟类资源的初步调查,共鉴定鸟类28种,隶属于5个目,15个科,25个属。公园内的Shannon-Wiener指数为0.864 6,均匀度指数为0.597 5。同时,用G-F指数对该地区鸟类的科与属的多样性指数进行了分析,得出G指数为1.374 5,F指数为1.586 2,G-F指数为0.133 5。与湖南其它几个地区的鸟类多样性相比,黑麋峰的鸟类多样性较低,并进一步分析了其鸟类多样性低的原因。  相似文献   

A 13CO2 Enrichment Experiment to Study the Synthesis Pathways of Polyunsaturated Fatty Acids of the Haptophyte Tisochrysis lutea     

Marine Remize  Frdric Planchon  Matthieu Garnier  Ai Ning Loh  Fabienne Le Grand  Antoine Bideau  Christophe Lambert  Rudolph Corvaisier  Aswani Volety  Philippe Soudant 《Marine drugs》2022,20(1)

The production of polyunsaturated fatty acids (PUFA) in Tisochrysis lutea was studied using the gradual incorporation of a ¹³C-enriched isotopic marker, ¹³CO₂, for 24 h during the exponential growth of the algae. The ¹³C enrichment of eleven fatty acids was followed to understand the synthetic pathways the most likely to form the essential polyunsaturated fatty acids 20:5n-3 (EPA) and 22:6n-3 (DHA) in T. lutea. The fatty acids 16:0, 18:1n-9 + 18:3n-3, 18:2n-6, and 22:5n-6 were the most enriched in ¹³C. On the contrary, 18:4n-3 and 18:5n-3 were the least enriched in ¹³C after long chain polyunsaturated fatty acids such as 20:5n-3 or 22:5n-3. The algae appeared to use different routes in parallel to form its polyunsaturated fatty acids. The use of the PKS pathway was hypothesized for polyunsaturated fatty acids with n-6 configuration (such as 22:5n-6) but might also exist for n-3 PUFA (especially 20:5n-3). With regard to the conventional n-3 PUFA pathway, Δ6 desaturation of 18:3n-3 appeared to be the most limiting step for T. lutea, “stopping” at the synthesis of 18:4n-3 and 18:5n-3. These two fatty acids were hypothesized to not undergo any further reaction of elongation and desaturation after being formed and were therefore considered “end-products”. To circumvent this limiting synthetic route, Tisochrysis lutea seemed to have developed an alternative route via Δ8 desaturation to produce longer chain fatty acids such as 20:5n-3 and 22:5n-3. 22:6n-3 presented a lower enrichment and appeared to be produced by a combination of different pathways: the conventional n-3 PUFA pathway by desaturation of 22:5n-3, the alternative route of ω-3 desaturase using 22:5n-6 as precursor, and possibly the PKS pathway. In this study, PKS synthesis looked particularly effective for producing long chain polyunsaturated fatty acids. The rate of enrichment of these compounds hypothetically synthesized by PKS is remarkably fast, making undetectable the ¹³C incorporation into their precursors. Finally, we identified a protein cluster gathering PKS sequences of proteins that are hypothesized allowing n-3 PUFA synthesis.  相似文献   

Generation of a uniform thymic malignant lymphoma model with C57BL/6J p53 gene deficient mice     

Susu Liu  Jianjun Lyu  Qianqian Li  Xi Wu  Yanwei Yang  Guitao Huo  Qingfen Zhu  Ming Guo  Yuelei Shen  Sanlong Wang  Changfa Fan 《Journal of toxicologic pathology》2022,35(1):25

Lymphoma is the third most common cancer diagnosed in children, and T-cell lymphoma has the worst prognosis based on clinical observations. To date, a lymphoma model with uniform penetrance has not yet been developed. In this study, we generated a p53 deficient mouse model by targeting embryonic stem cells derived from a C57BL/6J mouse strain. Homozygous p53 deficient mice exhibited a higher rate of spontaneous tumorigenesis, with a high spontaneous occurrence rate (93.3%) of malignant lymphoma. Because tumor models with high phenotypic consistency are currently needed, we generated a lymphoma model by a single intraperitoneal injection of 37.5 or 75 mg/kg N-methyl-N-nitrosourea to p53 deficient mice. Lymphoma and retinal degeneration occurred in 100% of p53^+/− mice administered with higher concentrations of N-methyl-N-nitrosourea, a much greater response than those of previously reported models. The main anatomic sites of lymphoma were the thymus, spleen, bone marrow, and lymph nodes. Both induced and spontaneous lymphomas in the thymus and spleen stained positive for CD3 antigen, and flow cytometry detected positive CD4 and/or CD8 cells. Based on our observations and previous data, we hypothesize that mice with a B6 background are prone to lymphomagenesis.  相似文献