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MOU Yong-gao XIE Hai-tao PENG Hui WANG Zhen-ning WEI Da-nian ZHANG Xiang-heng SAI Ke DENG Xing-hai ZHANG Guan-hua CHEN Zhong-ping 《园艺学报》2010,26(12):2342-2346
AIM: To investigate the expression of 4-1BBL(CD137L) protein in human glioma tissues, and to analyze its clinical significance. METHODS: The expression levels of 4-1BBL protein in 82 human glioma specimens were measured by the method of immunohistochemistry. The correlation of 4-1BBL protein level with histopathologic features and survival time was analyzed. RESULTS: 4-1BBL protein positive cells were observed in human brain astrocytic tumors, which were not found in meningioma and normal brain tissues. In our study, the expression of 4-1BBL protein in 3 cases of normal brain tissues was negative. The expression of 4-1BBL protein in 31 cases of glioma specimens were positive, and total positive rate was 37.8% (31/82). The expression of 4-1BBL protein was related to the survival time and age of the patients, but not related to the pathological grade of glioma. Statistically, the patients with positive expression of 4-1BBL protein had longer survival time (P<0.05). CONCLUSION: Expression of 4-1BBL protein may have reference value to predict the prognosis of patients with glioma. 相似文献
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AIM:To investigate the effects of microRNA (miRNA)-483-3p on the growth and migration of human glioma cell line A172 and its potential mechanisms.METHODS:The abundance of miRNA-483-3p in human embryonic kidney 293 cells and different human glioma cell lines (A172,U251 and SHG44) was measured by RT-qPCR.After down-regulation of miRNA-483-3p by transfection of inhibitor in the A172 cells,the cell viability,cell cycle distribution and cell migration were detected by CCK-8 assay,flow cytometry and Transwell assay,respectively.Furthermore,the protein levels of cell cycle-related molecules and epithelial-mesenchymal transition markers were measured by Western blot.Luciferase reporter assay was used to predict and verify the target gene of miRNA-483-3p.RESULTS:miRNA-483-3p was highly expressed in human glioma cells.Knockdown of miRNA-483-3p inhibited A172 cell viability,arrested cell cycle and decreased cell migration rate.Furthermore,the protein levels of cyclin D1,cyclin-dependent kinase 4,phoshorylated retinoblastoma protein,N-cadherin and vimentin were significantly decreased after knockdown of miRNA-483-3p,accompanied with the up-regulation of E-cadherin and β-catenin protein expression.Luciferase reporter assay demonstrated that Smad4 was a potential target gene of miRNA-483-3p.Down-regulation of Smad4 in the A172 cells transfected with miRNA-483-3p inhibitor partially reversed the effect of miRNA-483-3p on cell viability and migration.CONCLUSION:Knockdown of miRNA-483-3p restrains the growth and migration of A172 cells by targeting Smad4. 相似文献
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The tumor stem cell theory supposed that tumor stem cells are the origin of tumor abnormal proliferation, invasion, metastasis, drug resistance and recurrence. As the theory is put forward, it redefines the functions of classic stem cells in tumorigenesis. It is a great event for recent studies on glioma initiating cells as brain tumor stem cells were identified and isolated successfully. A lot of evidence from experiments in vivo and in vitro demonstrates that brain tumor stem cells might play an important role in glioma tumorigenesis. In this review, we discuss the relationship between tumor stem cells and tumorigenesis, and the research on the correlation between brain tumor stem cells and glioma genesis. 相似文献
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AIM To investigate the effect of niflumic acid （NFA） on human glioma U87 cells and to clarify the potential mechanism. METHODS The U87 cells were cultured in vitro and divided into blank control group， and 50， 100 and 200 μmol/L NFA groups. MTT assay was performed to determine the viability of cells in various groups. Migration and invasion abilities were measured by real-time cell analysis （RTCA）. RESULTS The results of MTT assay showed that compared with blank control group， the viability of U87 cells was increased after treatment with NFA for 12 h （P <0.05 or P <0.01）， while the viability was significantly decreased after treatment with NFA for 24 and 48 h （P <0.05 or P <0.01） in a concentration-dependent manner. The results of RTCA showed that compared with control group， the cell migration and invasion abilities were inhibited in 100 and 200 μmol/L NFA groups （P <0.05 or P <0.01） and the inhibitory effects were more obvious in 200 μmol/L NFA group （P <0.01）. CONCLUSION NFA inhibits the viability， migration and invasion of human glioma U87 cells. 相似文献
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AIM: To explore the effect of Vaccinium vitis procyanidin on the growth of glioma cells. METHODS: Glioma C6 cells were cultured and divided into control and 10, 20 and 40 μg/L Vaccinium vitis procyanidin groups. The influence of Vaccinium vitis procyanidin on the growth of C6 cells was measured by MTT assay and the observation under inverted microscope. The apoptotic rate was detected by Annexin V/PI staining. The protein expression of Bcl-2 and Bax was determined by immunocytochemistry. The protein levels of Bcl-2, Bax and caspase-3 were also examined by Western blotting. RESULTS: The growth of C6 glioma cells was inhibited by Vaccinium vitis procyanidin at concentrations of 10, 20 and 40 μg/L. The growth was significantly inhibited in 40 μg/L Vaccinium vitis procyanidin group at 24 h and 48 h, and in 20 and 40 μg/L Vaccinium vitis procyanidin groups at 72 h (P<0.01). The density of the cells was decreased when the concentration of Vaccinium vitis procyanidin increased. The apoptotic rate was increased when the concentration of Vaccinium vitis procyanidin increased either. The expression of Bcl-2 was decreased and Bax was increased after 10, 20 and 40 μg/L Vaccinium vitis procyanidin treatments. The ratio of Bax/Bcl-2 was increased when the dose of Vaccinium vitis procyanidin increased (P<0.05 or P<0.01). The expression of Bcl-2 was decreased (P<0.01), and Bax and caspase-3 were increased after 10, 20 and 40 μg/L Vaccinium vitis procyanidin treatments. The ratio of Bax/Bcl-2 was increased when the dose of Vaccinium vitis procyanidin increased (P<0.01). CONCLUSION: Vaccinium vitis procyanidin inhibits the growth of glioma cells by down-regulating Bcl-2 protein and up-regulating Bax protein to activate caspase-3, thus inducing apoptosis. 相似文献
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CHEN Yu LI Zhen-ye ZHANG Xiang-heng XIANG Jin SAI Ke KE Chao YANG Qun-ying GUO Jian-gui CHEN Zhong-ping MOU Yong-gao 《园艺学报》2013,29(5):810-814
AIM: To investigate the expression of B7-H4 in human glioma tissues and its clinical significance. METHODS: The histological staging of 150 cases of human glioma tissues was determined by HE staining. Immunohistochemistry was performed to study the protein level of B7-H4 in 150 human glioma specimens. Furthermore, the relationships between the expression level of B7-H4 and clinicopathological parameters, as well as patients survival rate, were analyzed. RESULTS: HE staining result indicated that there were 12 cases staged as stage I, 50 cases stage II, 39 cases stage III and 49 cases stage IV in 150 glioma specimens. Ninety-seven cases highly expressed B7-H4 in total 150 glioma samples with a 64.7% high expression rate. The histological grade of the tissues with high B7-H4 expression was mostly III~IV. There were 53 cases with low B7-H4 expression in the total 150 glioma patients with a 35.3% low expression rate. The histological grade of the tissues with low B7-H4 expression was mostly I~II. The B7-H4 expression was related to the age of the patients (P<0.01) and the pathological grade of glioma (P<0.01), but not related to the location of glioma (P>0.05). Kaplan-Meier survival curves demonstrated that increased B7-H4 expression was associated with shorter overall survival time (P<0.01). Multivariate Cox regression analysis revealed that B7-H4, age, sex and pathological grade were independent prognostic factors in glioma patients. CONCLUSION: B7-H4 is expressed in most of glioma tissues. B7-H4 may be a novel prognostic biomarker and a new target of molecular therapy for gliomas. 相似文献
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WANG Kui DU Xiu-lian CHANG Yan-zhong LI Rong-chang SZETO Ke Yee Katie SUN Hong-zhe QIAN Zhong-ming 《园艺学报》2003,19(7):902-904
AIM:To investigate the role of transferrin/transferrin receptor system in transferrin-bound Yb2 (Yb2Tf) uptake by U-87 MG cells and the effect of transferrin-bound and -free Yb2 on proliferation of U-87 MG cells.METHODS:Cell culture and ICP-MS measurement of Yb2.RESULTS:Yb2Tf uptake by U-87 MG cells increased with the concentrations of Yb2Tf, and reached saturation as the concentration in the incubation medium was raised to about 2 μmol/L. Also, Yb2 uptake by the cells increased with increase of the mole ratio (Yb2: apoTf), reaching a maximum at 1.5 mole ratio. Yb2Tf in 0.4 μmol/L significantly inhibited proliferation of U-87 MG cells, however, 10 μmol/L Yb3+ had no significant effect on proliferation of the cells.CONCLUSION:The uptake of Yb2 by U-87 MG cells might be mediated by transferrin/transferrin receptor system. Transferrin-bound but not transferrin-free Yb2 could significantly inhibit proliferation of U-87 MG cells. 相似文献
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AIM:Three different antisense oligonucleotides complementary to basic fibroblast growth factor (bFGF) mRNA were compared in inhibitory effect on gene targeted expression.METHODS:After transfecting bFGF antisense oligonucleotides (asODN) into SWO-38 cells by lipofectin, the proliferation of cells was identified by MTT method, apoptosis was examined by flow cytometric cell cycle analysis and the expression levers of bFGF were detected by Western-blotting.RESULTS:There were 49%, 33%, 51% inhibition of cell growth and 35%, 27%, 18% cell apoptosis after asODN1, asODN2 and asODN3 treatment.In addition, the decrease in bFGF protein was 63%, 42%, 11%, respectively.CONCLUSION:The data suggeste that asODN1 is a potent target to bFGF mRNA, which inhibits cell growth and induces apoptosis in SWO-38 cells. 相似文献
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AIM: To study the effects of VEGF over-expressed C6 glioma cells on the expression of Flk-1and Flt-1in cocultured microvascular endothelial cells using the rat hepatic cells BRL 3A as control. METHODS: Cocultured systems of rat pulmonary microvascular endothelial cells with C6 and endothelial cells with BRL 3A were established. Immunocytochemical method was used to investigate the expression change of Flk-1and Flt-1protein in cocultured microvascular endothelial cells. The expression of Flt-1and Flk-1mRNA was analyzed by RT-PCR and Northern blot. RESULTS: The microvascular endothelial cells cocultured with C6 showed increased expression of Flk-1and Flt-1protein (P <0.05), while that cocultured with BRL 3A, showed decreased expression of Flt-1and Flk-1protein(P <0.01). The results of RT-PCR and Northern blot showed that the Flk-1, Flt-1mRNA of microvascular endothelial cells were markedly up-regulated after coculture with C6 glioma cells (P <0.01), while the endothelial cells cocultured with BRL 3A had down-regulated expression of Flk-1, Flt-1mRNA (P <0.01). CONCLUSION: VEGF over-expressed C6 glioma cells may markedly up-regulate the expression of Flt-1and Flk-1in cocultured microvascular endothelial cells. These results suggest that this effect could be one of the important mechanisms in glioma angiogenesis in vivo. 相似文献