Cloning of plasma membrane H-ATPase gene in Populus euphratica Oliv.     

Ning De-juan Hou Pei-chen Hu Zan-min Shen Xin Chen Shao-liang College of Biological Sciences  Biotechnology  Beijing Forestry University  Beijing  P. R. China Institute of Genetics  Developmental Biology  Chinese Academy of Sciences  Beijing  P. R. China 《中国林学(英文版)》2006,8(4):15-19

For this paper, the plasma membrane (PM) H -ATPase gene has been cloned from Populus euphratica Oliv. through a homology based strategy. The isolated 3,210 bp cDNA contains a single 2,862 bp open reading frame (ORF) which encodes a putative H -ATPase protein of 953 amino acid residues, with a significant homology to plasma membrane H -ATPase of Primus persica, Phaseolus vulgaris, Sesbania rostrata and Daucus carota. The predicted protein has a molecular weight of 104,553 Da. The copy number analysis revealed multiple copies of the PM H -ATPase in the P. euphratica genome after digestion of their genomic DNA by the restriction enzymes EcoRI, NdeⅠ, FbaⅠand BglⅡ, and Southern blot.  相似文献   

通过农杆菌介导法将口蹄疫结构蛋白全长基因P1导入大豆的研究     

王萍  郭永来  王罡  季静  余云舟  李昌  金宁一 《西南农业学报》2005,18(5):643-646

本研究以大豆体细胞胚为受体。用农杆菌介导法将口蹄疫病毒结构蛋白全长基因P1导入大豆基因组中．获得了抗性植株。经GUS染色、PCR及PCR-southern杂交等分子检测。证明目的基因已导入并整合到大豆基因组中。为利用大豆作为生物反应器生产口蹄疫新型口服疫苗奠定了基础。  相似文献   

应用积分GM(1,1)模型进行杉木炭疽病预测预报的研究   总被引：1，自引：0，他引：1  

朱建华  叶剑雄  张再福  陈红梅  傅瑞树 《福建林业科技》2002,29(1):23-25

以积分生成代替传统的累加生成方法 ,并用双向差分建立积分GM (1,1)灰色预测模型进行杉木炭疽病预测预报的研究 ,结果表明积分GM (1,1)模型能较好地预测杉木炭疽病在三明市的发生  相似文献   

Effect of Nitrogen Rate and Stubble Height on Dry Matter Yield,Crude Protein Content and Crude Protein Yield of a Sorghum–Sudangrass Hybrid[Sorghum bicolor (L.) Moench × Sorghum sudanense (Piper) Stapf.] in the Three‐Cutting System     

S. Iptas  A. R. Brohi 《Journal of Agronomy and Crop Science》2003,189(4):227-232

In this study, the effects of nitrogen (N) rate (60, 120, 180 and 240 kg N ha^?1 applied in three equal dressings at seeding and after the first and second cuttings) and stubble height (7, 14 and 21 cm) on the dry matter (DM) yield, crude protein (CP) content, and CP yield of a sorghum–sudangrass hybrid [Sorghum bicolor (L.) Moench × Sorghum sudanense (Piper) Stapf., cv. Pioneer 988] in the three‐cut system was investigated. The N rate had no significant effect in the first and third cuttings, but in the second cutting DM yields increased significantly with increase in N rate. The highest yield of 9.1 t ha^?1 was obtained with 80 kg N ha^?1 for the average of 2 years at the second cutting, but no significant difference was found among the 40, 60 and 80 kg N ha^?1 rates. CP content and yield were not significantly affected by N rate at the first and third cuttings, but CP content and yield were significantly affected by application of N at the second cutting. Stubble height had a significant effect on CP content at the third cutting. However, it had no significant effect on CP content at the first and second cuttings. Stubble height had a significant effect on the CP yield at the first cutting, but no significant effect on CP yield at the second and third cuttings.  相似文献   

干椒1号辣椒的选育     

陈炳金 《辣椒杂志》2003,(3):20-21

干椒1号是由母本二金条-12-6-4和父本羊角-2-3配制的一代杂交种。该品种早熟，高抗病毒病、疫病，耐寒、耐热、耐湿、耐瘠。果实细长羊角形，青椒绿色，老熟果鲜红色，果皮薄、籽少、味辣，易脱水，晒干率高，宜作晒干椒或鲜食。一般667m^2产鲜红椒2500kg，或干椒800kg。全国各地均宜种植，特别适宜嗜辣、习惯晒干椒的地区大面积推广。  相似文献   

猪肺炎支原体黏附因子基因R1R2区的克隆及表达   总被引：4，自引：0，他引：4  

张映  贾广乐  邵国青  聂向庭 《中国兽医学报》2003,23(4):338-341

根据GenBank登录的猪肺炎支原体232株P97基因和J株黏附因子基因设计了1对引物，以我国猪肺炎支原体Z株(强毒株)基因组DNA为模板，通过PCR方法扩增了该株黏附因子基因的部分序列。经序列分析后，重新设计了1对带有EcoRI和HindⅢ酶切位点的引物，并经引物的定点突变，PCR扩增了Z株黏附因子的R1R2区。扩增产物经双酶切后克隆到表达载体pET-32(a) 中。该重组质粒经酶切鉴定后，将其具有正确阅读框架的重组质粒转化到大肠杆菌BL21(DE3)感受态细胞，37℃下经IPTG诱导表达，得到相对分子质量约29000的融合蛋白，表达量约为11％。  相似文献   

木质纤维素的降解和五种酶的活性对香菇子实体形成的影响   总被引：6，自引：1，他引：5  

潘迎捷  郑志仁 《食用菌学报》1996,3(3):25-30

本文从生化角度研究了F型(形成子实体)和N型(不形成子实体)两类香菇菌株在生长发育过程中生化代谢活动上的差异。结果表明,在香菇的整个生长过程中,F型菌株的纤维素酶、半纤维素酶、漆酶、酸性蛋白酶和果胶酶的活性均高于N型菌株,尤其在形成子实体的第二和第三阶段,纤维素酶、半纤维素酶和酸性蛋白酶的活性均呈较大幅度的增加,这种趋势与胞外可溶性蛋白含量的增幅是一致的,同时F型菌株对培养基中木质素、纤维素和半纤维素的降解能力也高于N型菌株,说明在形成子实体的过程中,F型菌株具有比N型菌株相对更强的生化代谢能力;各种与物质降解有关酶的代谢活动在某些环节上受阻或不能有效地表达是N型菌株不能形成子实体的一个重要生理原因。  相似文献   

水分对硝酸磷肥中氮的扩散及其肥效的影响     

黄瑞珍  张胜 《中国农业大学学报》1989,(2):193-200

国产硝酸磷肥含氮27%,磷13.5%。所含速效氮中硝态氮均占一半。研究麦田不同水分对硝酸磷肥中硝态氮在土壤中扩散结果显示,硝态氮在土壤中下移深度与土壤湿润峰的深度基本一致,在小麦越冬期有表聚现象。当灌水量为田间持水量时,硝态氮移动深度为40～50cm,是在主要根系活动层内,这对小麦吸收有利,不会产生氮的流失。等氮磷条件下,硝酸磷肥与磷铵加尿素在北方地区有同样肥效。  相似文献   

夏大豆亩产262.1kg生理指标研究   总被引：4，自引：1，他引：3  

卢增辉  赵俊杰 《大豆科学》1994,13(3):185-192

研究了夏大豆中油－８９Ｄ亩产２６２．１ｋｇ各项生理指标：叶面积系数，开花期、结荚期、鼓粒期分别为３．９８、５．８０、４．９１；总光合势１８．２万ｍ＾２·日，平均净光合生产率４．８１ｇ／ｍ＾２·日，氮磷总积累量分别为２２．２８ｋｇ和３．１３ｋｇ，每生产１００ｋｇ籽粒需吸收氮８．５０ｋｇ，磷１．１９ｋｇ，总干物质积累８７６．４７ｋｇ，粒茎比０．５１，经济系数０．３０。  相似文献   

Effect of Integration of Fertilizer and Green Manure Nitrogen on Yield Attributes, Nitrogen Uptake and Yield of Lowland Rice (Oryza sativa L.)     

M. N. Budhar  S. P. Palaniappan 《Journal of Agronomy and Crop Science》1996,176(3):183-187

Field experiments were conducted at Tamil Nadu Rice Research Institute, Aduthurai, India, during the wet seasons of 1992 and 1993 to study the effect of full and partial substitution of fertiliser N with green manure N (Sesbania rostrata) on nitrogen uptake, yield attributes and yield of rice. The experiment consisted of eight treatments with two levels of N (100 and 200 kg ha⁻¹) and three sources of N application viz., fertilizer, integrated (1:1 fertilizer and green manure N) and green manure N compared to the recommended practice (150 kg fertilizer plus 6.25 t ha⁻¹ (72 kg N) green manure) and a no N control. Nitrogen application markedly increased the N uptake. Combined use of the two N sources at 200 and 222 kg N ha⁻¹ and of single fertilizer N at 200 kg N ha⁻¹ recorded the maximum N uptake, increased the yield attributes such as number of panicles per unit area, weight per panicle, number of total and filled grains per panicle and test weight. At 200 kg N ha⁻¹ full substitution of N by green manure reduced the grain yield but only partial substitution of N by green manure resulted in almost similar yield as single fertilizer N. Thus 200 kg N ha⁻¹ applied in equal proportions of fertilizer and green manure N can be recommended for medium duration rice cultivars.  相似文献