大豆脂肪酸脱氢酶GmFAD3C-1基因的克隆及功能分析     

焦苏淇  周俊名  尚雨晴  王佳欣  张爱晶  何浩博  赵秋竹  李越  姚丹 《中国油料作物学报》2022,44(5):1006

研究通过对大豆脂肪酸脱氢酶GmFAD3家族中4个关键酶基因序列进行比对,与对照JN18相比,大豆低亚麻酸突变体MT72中GmFAD3C-1基因在起始密码子后+966bp处存在一个碱基位点的缺失（G→?）,产生移码突变,导致氨基酸序列上产生较大变化（该突变基因命名为gmfad3c-1）。构建GmFAD3C-1基因超表达及CRISPR/Cas9编辑载体,利用花粉管通道法获得T₂转化植株。脂肪酸脱氢酶酶活测定结果显示,超表达植株T₂籽粒中,酶活与对照相比上升47.62%~78.85%,编辑载体T₂籽粒中,酶活与对照相比下降25.67%~47.11%。脂肪酸相对含量测定的结果进一步表明,GmFAD3C-1基因的表达与植株亚麻酸含量密切相关。  相似文献   

ω-黑麦碱基因表达缺失的1BL/1RS易位系种质创新     

张士昌  李亚青  张楠  彭义峰  李孟军 《麦类作物学报》2022,(1):11-16

1BL/1RS易位系是小麦育种的重要亲本,但1RS Sec-1位点表达的ω-黑麦碱是导致小麦加工品质不佳的重要因素。为消除ω-黑麦碱的不良影响,进一步挖掘1BL/1RS易位系的育种潜力,本研究利用低能N+离子束处理1BL/1RS易位系小麦干种子,利用醇溶蛋白酸性聚丙烯酰胺凝胶电泳(A-PAGE)筛选ω-黑麦碱表达缺失突变株系,1RS染色体特异引物鉴定携带1RS染色体的突变株系,结果共创制出9个ω-黑麦碱基因Sec-1位点表达缺失的新的1BL/1RS易位系种质。农艺性状和品质性状分析结果表明,这些1BL/1RS易位系新种质的籽粒蛋白含量、面筋指数、稳定时间等加工品质性状显著提高,株高、穗数、千粒重等农艺性状无显著变化。ω-黑麦碱表达缺失突变体的育成为培育优质抗逆小麦品种提供了新的方法。  相似文献   

硬枝碱蓬HPLC指纹图谱建立及模式识别研究     

马涛  赵宇  韩占江 《塔里木大学学报》2014,(4):96-104

为了对硬枝碱蓬样品质量进行评价,采用高效液相色谱法对9个不同采集地的硬枝碱蓬样品进行测定,建立指纹图谱,并结合中药相似度软件和主成分分析法进行模式识别研究。结果表明,试验确定的最佳条件为:提取溶剂为100%甲醇,用量为15 m L,流速为1.0 m L/min,色谱进样时间为20 min,流动相为甲醇(A)-水(B),采用100%甲醇等度洗脱,检测波长为254 nm。精密度试验、重现性试验、稳定性试验测定结果的相似度均在0.9以上,本研究所建立的基于主成分分析的模式识别方法准确可靠;中药相似度软件能够对不同地点的硬枝碱蓬材料进行区分,不同产地硬枝碱蓬的差异可清晰地显示出来。  相似文献   

改良S1选择法对12个玉米群体改良效果的比较分析     

孙峰成  冯勇  付增娟  苏二虎  张来厚  赵瑞霞  刘志雄  石海波  李美娜 《农业科学与技术》2014,(10):1687-1692

为了对玉米群体进行合理有效的改良利用,通过改良S1选择法对12个玉米群体改良一轮后进行效果比较分析。结果表明,穗行数、行粒数和百粒重是构成产量的三因素,遗传增益最大的群体为蒙群2,其它依次为蒙群4、蒙群1等7个群体,蒙群3及3个国外引进群体遗传增益为负值;玉米群体在穗部、植株性状以及遗传变异中均存在一定的变化。试验研究得出,蒙群2、蒙群4、中综7号、蒙 C群、蒙 A群产量遗传增益均较大,并在其他农艺性状中改良效果显著,可直接选择优株自交进行选系,而其余7个群体遗传增益小,改良利用潜力不大。  相似文献   

慢病毒生产卵泡抑素转基因绵阳PCR检测方法的建立(英文)     

张宁  王晨懿  张雪梅  刘晨曦  贺三刚  李文蓉  刘明军 《农业科学与技术》2014,(7):1095-1099

[目的]建立稳定快速的慢病毒转基因羊DNA水平检测方法。[方法]采集初生转基因羔羊的子叶、脐带、尾组织以及死亡羔羊的全身各组织,提取基因组DNA作为模板进行PCR检测,以Follistatin基因N+D1片段特异性引物进行PCR检测;同时,对慢病毒载体的CMV启动子、5’-LTR等结构元件进行PCR检测;提取死亡羔羊的全身组织以及转基因羔羊的活体肌肉组织总RNA,进行RTPCR检测。[结果]采用试剂盒提取的基因组DNA比常规方法提取的DNA快速、高效,且更易被检测,扩增时的引物采用载体上游与目标基因下游的组合大大增加了检测的特异性,避免了内源性目标基因的干扰而造成假阳性;初生转基因羔羊的尾组织可作为PCR检测的组织样品,其结果可靠、准确;对CMV启动子及5’-LTR等结构元件作PCR检测为转基因动物的安全性考察提供了数据,同时验证了转基因羔羊目的基因的检测结果;对转基因羔羊肌肉组织提取总RNA后进行RT-PCR检测,其中有3只羔羊没有检测到转录产物,其他均有转录。[结论]该研究建立的转基因绵羊PCR检测方法高效、快速且准确,为进一步蛋白水平检测提供试验依据,也为建立转基因绵羊系统性多层次的检测方法奠定基础,还为转基因绵羊的安全监测提供了稳定的技术平台。  相似文献   

台农1号芒果一次花和二次花的特性研究     

邢福甫  吉福桑  王令霞  李新国  曾丽萍 《安徽农学通报》2014,(22):62-64

为了探讨比较台农1号芒果“一年两熟”栽培过程中一次花和二次花的形态特性,对其一次花和二次花的花序长度、花朵直径、两性花比率、花粉活性、花粉量及花粉形态、大小进行了试验研究。结果表明：一次花和二次花的花序平均长度分别为30.74cm和20.67cm;一次花和二次花的直径平均长度分别为7.30mm和6.55mm;一次花和二次花的两性花比率较低,一次花的两性花比率为20.06%,二花的两性花比率为28.79%;一次花的花粉活性比二次花的花粉活性高,一次花的花粉活性为12.90%,二次花的花粉活性为7.63%;一次花的每花花药含花粉量为500个,二次花的每花花药含花粉量为333个;台农1号一次花和二次花的花粉形态可分为长椭圆形花粉、椭圆形花粉、近圆形花粉和不规则形花粉4个类型。  相似文献   

胰腺炎型鸭1型甲肝病毒结构蛋白VP1基因的克隆和表达     

傅秋玲  傅光华  陈红梅  施少华  陈珍  程龙飞  万春和  林建生  黄瑜 《福建农业学报》2014,(5):409-412

通过RT-PCR方法扩增出MPZJ1206株胰腺炎型鸭1型甲肝病毒结构蛋白VP1基因,将其与原核表达载体pGEX-6P-1连接获得重组表达质粒pGEX-VP1,进行条件优化诱导表达,将表达的重组蛋白经SDS-PAGE分析。结果表明,分子量约为52 kDa的重组目的蛋白得到表达。该研究为开发胰腺炎型鸭1型甲肝病毒诊断方法和研究VP1蛋白功能奠定基础。  相似文献   

Relationship Between Leaf C/N Ratio and Insecticidal Protein Expression in Bt Cotton as Affected by High Temperature and N Rate     

ZHANG Xiang  Lü Chun-hua  CHEN Yuan  WANG Gui-xia  CHEN Yuan  CHEN De-hua 《中国农业科学(英文版)》2014,(1):82-88

Expression of insecticidal protein for transgenic Bacillus thuringiensis （Bt） cotton is unstable and related to nitrogen metabolism. The objective of this study was to investigate the relationship between leaf carbon nitrogen ratio （C/N） and insecticidal efficacy of two Bt cotton cultivars. C/N ratio and Bt protein content were both measured at peak square period and peak boll period respectively under 5-7 d high temperature and different nitrogen fertilizer rates on the Yangzhou University Farm and the Ludong Cotton Farm, China. All plants were grown in field. The results showed that the C/N ratio enhanced slightly and the Bt protein content remained stable at peak square period, but significant increases for the C/N ratio and decreases markedly for the leaf Bt protein concentration were detected at the peak boll period. The similar patterns at the two growth periods were found for the leaf C/N ratio and Bt protein content by different N fertilizer treatments. When nitrogen rate was from 0 to 600 kg ha-l, the C/N ratio was reduced by 0.017 and 0.006 for Sikang 1 and Sikang 3 at peak square period, compared to the 1.350 to 1.143 reduction for Sikang 1 and Sikang 3 at peak boll period, respectively. Correspondingly, the leaf Bt protein contents were bolstered by 2.6-11.8 and 26.9-36.9% at the two different growth periods, respectively. The results suggested that enhanced C/N ratio by high temperature and nitrogen application may result in the reduction of inseetiocidal efficacy in Bt cotton, especially in peak boll period.  相似文献   

Effect of Biogas Digested Liquid on OH4 and N2O Flux in Paddy Ecosystem     

Ankit Singla  Kazuyuki Inubushi 《中国农业科学(英文版)》2014,(3):635-640

Biogas production generates digested slurry as a byproduct. It can be used as a fertilizer especially after its conversion into digested liquid. A pot based study was conducted in order to evaluate the effect of the application of digested liquid on CH4 and N2O flux, and plant biomass in paddy. Analysis revealed that digested liquid treated soils released more CH4 compared to ammonium sulphate and the control. Ammonium sulphate treated soil emitted the highest N20 whereas digested liquid application decreased its emission significantly. Further, the cumulative emission over 101 d of the experiment was found to be higher for CHa （16.9 to 29.9 g m^-2） compared to N20 （-49.3 to 18.9 mg m^-2） for all treatments. Digested liquid application had positive impact on plant variables such as panicle number and weight of panicles. This study suggests that digested liquid application significantly decrease N20 emission and increase CH4 emission possibly due to affecting the availability of organic C in the soil to microbial activity for methanogenesis. Another possibility for enhancing CH4 emission by following biogas digested liquid could be attributed to the increase in plant biomass.  相似文献   

Nitrogen Deficiency Limited the Improvement of Photosynthesis in Maize by Elevated CO₂ Under Drought     

ZONG Yu-zheng  SHANGGUAN Zhou-ping 《中国农业科学(英文版)》2014,(1):73-81

Global environmental change affects plant physiological and ecosystem processes. The interaction of elevated CO2, drought and nitrogen （N） deficiency result in complex responses of C4 species photosynthetic process that challenge our current understanding. An experiment of maize （Zea mays L.） involving CO2 concentrations （380 or 750 μmol mol1, climate chamber）, osmotic stresses （10% PEG-6000, -0.32 MPa） and nitrogen constraints （N deficiency treated since the 144th drought hour） was carried out to investigate its photosynthesis capacity and leaf nitrogen use efficiency. Elevated CO2 could alleviate drought-induced photosynthetic limitation through increasing capacity of PEPC carboxylation （Vp~,x） and decreasing stomatal limitations （SL）. The N deficiency exacerbated drought-induced photosynthesis limitations in ambient CO2. Elevated CO2 partially alleviated the limitation induced by drought and N deficiency through improving the capacity of Rubisco carboxylation （Vmax） and decreasing SL. Plants with N deficiency transported more N to their leaves at elevated CO2, leading to a high photosynthetic nitrogen-use efficiency but low whole-plant nitrogen-use efficiency. The stress mitigation by elevated CO2 under N deficiency conditions was not enough to improving plant N use efficiency and biomass accumulation. The study demonstrated that elevated CO2 could alleviate drought-induced photosynthesis limitation, but the alleviation varied with N supplies.  相似文献