Cytological grading of canine cutaneous mast cell tumours          下载免费PDF全文

Filippo Scarpa  Silvia Sabattini  Giuliano Bettini 《Veterinary and comparative oncology》2016,14(3):245-251

A cytological grading for mast cell tumours (MCTs) would be highly desirable, allowing to select the most appropriate therapeutic intervention prior to surgery. This study evaluates the applicability on fine‐needle aspirations (FNAs) of the novel Kiupel grading system, based on number of mitoses, multinucleated cells, bizarre nuclei and presence of karyomegaly. Fifty consecutive cases with pre‐operative cytological diagnosis were included. In cytological specimens, approximately 1000 cells were evaluated, and the histological grade was assessed on the corresponding resected specimens. On cytology, the above parameters were significantly different between histologically low‐grade and high‐grade tumours (P < 0.001). The cytograding correctly predicted the histological grade in 47 cases (accuracy, 94%; sensitivity, 84.6%; specificity, 97.3%). Two high‐grade MCTs (4%) were not detected on cytology. The cytograding can provide helpful insights to assist clinical decisions in most cases. However, the risk of underestimation in a minority of patients represents a limit to the overall utility of the technique.  相似文献   

Acute‐phase proteins as diagnostic markers in horses with colic          下载免费PDF全文

Tina H. Pihl DVM  PhD  Elrien Scheepers BVSc  MSc  Macarena Sanz DVM  MS  DACVIM  Amelia Goddard BVSc  MMed Vet  Nils Toft Cand Sci  PhD  Mads Kjelgaard‐Hansen DVM  PhD  Pia H. Andersen DVM  PhD  DVSc  Stine Jacobsen DVM  PhD 《Journal of Veterinary Emergency and Critical Care》2016,26(5):664-674

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Equine platelet lysate as an alternative to fetal bovine serum in equine mesenchymal stromal cell culture – too much of a good thing?          下载免费PDF全文

K. A. Russell  T. G. Koch 《Equine veterinary journal》2016,48(2):261-264

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Surgery of the Sinuses and Eyes     

《Veterinary Clinics of North America: Food Animal Practice》2016,32(3):571-591

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A spontaneously occurring malignant ovarian Sertoli cell tumor in a young Sprague Dawley rat     

Yuichi Kinoshita  Katsuhiko Yoshizawa  Yuko Emoto  Michiko Yuki  Takashi Yuri  Nobuaki Shikata  Susan A. Elmore  Airo Tsubura 《Journal of toxicologic pathology》2016,29(1):53-59

Primary ovarian tumors are generally uncommon in rats used in toxicologic studies. A malignant Sertoli cell tumor was present in the ovary of a 19-week-old female Sprague Dawley rat. Macroscopically, the mass was white and firm, 10 × 13 × 17 mm in size, and located in the right ovary. Histopathologically, the mass was composed of nests of pleomorphic cells, which formed seminiferous-like tubules separated by a thin fibrovascular stroma. The tubules were lined by tumor cells, which had basally located nuclei and abundant eosinophilic and vacuolated cytoplasm. In some areas, the tumor cells were arranged in a retiform growth pattern, mimicking a rete testis/ovarii. Disseminated metastases to the surfaces of the mesentery, spleen and liver were also present. Immunohistochemically, many tumor cells were strongly positive for vimentin, estrogen receptor α and Ki 67. Some tumor cells were positive for pancytokeratin and inhibin α. These findings closely resemble those of an ovarian-derived human malignant Sertoli cell tumor. From our review of the literature, we believe this is the first report of a spontaneous malignant Sertoli cell tumor in the ovary of a young laboratory rat. This case might provide useful historical control information for rat toxicity studies.  相似文献   

CoCl2对Na2CO3胁迫下苜蓿幼苗叶片细胞膜的保护作用   总被引：6，自引：3，他引：3  

李朝周 《草业学报》2007,16(3):49-54

本研究报道了CoCl2对Na2CO3胁迫下苜蓿幼苗叶片细胞膜的保护作用及机制,研究结果表明,在120mmol/L Na2CO3胁迫的前期,苜蓿幼苗叶片多胺含量及抗氧化酶活性显著升高,过氧化物酶除外,而活性氧水平、脂质过氧化程度和细胞膜相对透性无显著变化;当120 mmol/L Na2CO3胁迫延长至24~28 d时,乙烯产生速率大幅度上升,多胺氧化酶及二胺氧化酶活性显著升高,精胺、亚精胺和腐胺含量显著下降;抗氧化酶活性显著下降,活性氧水平与脂质过氧化程度显著提高,细胞膜稳定指数大幅度下降。在120 mmol/L Na2CO3溶液中加入CoCl2至50μmol/L,在胁迫的整个过程中乙烯产生被显著抑制,同时相对提高了抗氧化酶的活性,并在胁迫后期显著减轻了多胺氧化酶活性升高、多胺含量下降及脂质过氧化程度的增加,减缓了细胞膜稳定指数的下降,从而对Na2CO3胁迫下苜蓿幼苗叶片细胞膜表现出一定的保护作用。  相似文献   

In vivo confocal microscopy in the normal corneas of cats, dogs and birds   总被引：1，自引：1，他引：0  

Kafarnik C  Fritsche J  Reese S 《Veterinary ophthalmology》2007,10(4):222-230

OBJECTIVE: To evaluate the applicability of in vivo confocal microscopy (IVCM) in veterinary ophthalmology and analyze the morphology of living, healthy cornea. ANIMALS EXAMINED: Thirty-seven dogs, 34 cats and five birds. PROCEDURE: Various corneal sublayers were visualized in the central region using an in vivo confocal corneal microscope (HRTII/RCM). RESULTS: An investigation method was developed and adapted for use on animals with varying skull forms and eye positions. Real-time images of the epithelial cells, the corneal stroma and the endothelial layer were obtained. The corneal stromal nerve trunks and the subepithelial and basal epithelial nerve plexus were visualized. In dogs, full corneal thickness (FCT) was 585 +/- 79 microm (mean +/- SD) and endothelial cell density (ECD) 3175 +/- 776 cells/mm(2) (mean +/- SD). In cats, FCT was 592 +/- 80 microm and ECD 2846 +/- 403 cells/mm(2). There were no significant differences between canine and feline FCT and ECD and no morphologic differences could be seen between dogs and cats. The bird images revealed a number of structural differences. CONCLUSION: Noninvasive IVCM allows accurate detection of corneal sublayers, corneal pachymetry, endothelial cell density and corneal innervation in various animal species. For clinical usage, patients must be under general anesthesia. The confocal images provided anatomic reference images of various healthy corneal structures in dogs, cats and birds.  相似文献   

苜蓿原生质培养及体细胞杂交技术的应用   总被引：1，自引：1，他引：0  

马晖玲  卢欣石  曹致中 《草业科学》2007,24(8):64-68

阐述了植物原生质体培养和体细胞杂交技术以及苜蓿Medicago spp.体细胞胚胎发生特性,综述了该技术在苜蓿育种研究中的应用状况,同时介绍了我国苜蓿研究领域原生质体培养和体细胞杂交技术的研究进展.  相似文献   

基于最大熵模型预测小火蚁在我国的适生区     

徐强  顾渝娟  李盼畔  武目涛  魏霜  刘海军  马骏 《植物保护》2023,49(4):101-107

小火蚁是中国大陆新发现的重要外来入侵害虫, 目前对该虫的传入来源和在我国的适生区范围尚不明确?为明确我国小火蚁适生区范围, 有效防控该虫在我国的扩散和蔓延, 本研究通过该虫全球已有的分布数据, 采用最大熵模型对其适生区进行了预测?研究表明, 最大熵模型预测小火蚁适生区精度较高?预测结果显示, 我国小火蚁的潜在适生区主要分布于南方, 其中, 高适生区分布在台湾?海南?云南南部边境?广西西南局部?福建西南部?广东南部及其沿海地区?预测结果与该物种现有地理范围的生态条件一致?年降水量对小火蚁的适生性影响最大, 理论年降水量为2 040 mm时小火蚁分布的概率最高?随着全球气候变暖, 未来我国小火蚁的适生区有向北扩大的趋势, 但主要适生区还是以南方为主?  相似文献   

两种沉水植物在不同水深和生长时期对入侵植物的响应     

阿斯哈  高海燕  沈一娈  孙凯  薛晶晶  蔡竟芳  程蕊  杨丽娟  李红丽 《植物保护学报》2023,50(6):1561-1570

为研究沉水植物在不同水深和生长时期对不同种类入侵植物的响应,选取2种湿地入侵植物凤眼莲Eichhornia crassipes、水盾草Cabomba caroliniana和2种常见本地沉水植物黑藻Hydrillaverticillata、菹草Potamogeton crispus为研究对象,通过模拟试验探究在不同水深（0.2、0.4 m）和生长时期（生长初期和生长旺期）的本地沉水植物对2种入侵植物的响应。结果显示,当水深为0.4 m时更有利于黑藻的生长,并可削弱入侵植物对黑藻生长的消极作用,而水深对菹草的生长无显著影响;入侵植物种类对本地沉水植物的生长无显著影响,但影响方式却存在差异,其中水盾草倾向于直接抑制本地沉水植物的生长,而凤眼莲可以通过降低水体透明度以及总磷含量进而间接抑制本地沉水植物的生长。此外,外来植物在本地沉水植物生长初期入侵对本地沉水植物产生的消极影响较生长旺期入侵时更显著。表明凤眼莲及水盾草入侵对本地沉水植物生长的影响机制存在差异,本地沉水植物在适宜水深和生长旺期对2种入侵植物具有更强的抵抗能力。  相似文献