有氧运动通过上调IGF1改善抑郁症大鼠的学习记忆能力     

刘红军  吕毓虎 《上海交通大学学报(农业科学版)》2014,(3):101-106

目的：分析4周有氧运动对抑郁症大鼠海马组织胰岛素样生长因子-1(insulin-like growth factor-1, IGF1) 信号通路的影响,探究长期有氧运动缓解抑郁症及改善机体记忆学习能力的分子生物学机制。方法：30只成年雄性清洁级SD大鼠（250g～300g）诱导慢性不可预见性应激模型。建模成功的SD大鼠随机分为抑郁安静组 (Con) 和抑郁有氧运动组 (ET) 各10只。ET 组小鼠进行为期4周的有氧跑台训练,抑郁安静组的大鼠笼内自由活动作为对照。4周后取两组大鼠海马组织,进行基因芯片分析;随机选取6个差异表达基因进行Real-time qPCR实验以验证基因芯片结果; Western Blot实验检测IGF1及其信号通路相关分子和细胞外信号调节激 酶 (extracellular signal-regulated kinase, ERK)、环磷酸腺苷反应元件结合蛋白(cAMP response element binding protein, CREB)的表达。结果：对比 Con 和 ET 组SD大鼠海马组织基因表达谱,筛选发现共有366个差异表达基因,其中表达上调的基因有 332 个,表达下调的基因有 34 个。 Real-time qPCR实验结果与基因芯片一致。通路 (Pathway) 分析结果显示,差异表达基因编码的蛋白共富集 61 条细胞信号通路,其中包括IGF1信号通路。采用Western Blot检测IGF1信号通路相关分子,发现ET 组IGF1,ERK及CREB表达上调。结论：4周有氧跑台训练引起抑郁症SD大鼠海马组织全基因表达谱发生变化,可能通过调控IGF1信号通路相关分子促进机体记忆学习能力的提高。  相似文献   

Promoter-specific expression of the imprinted IGF2 gene in bovine oocytes and pre-implantation embryos     

Bruna Rodrigues Willhelm  Elvis Ticiani  Karine Campagnolo  Gabriella Borba de Oliveira  Karine de Mattos  Camilo Andrés Peña Bello  Felipe Ledur Ongaratto  Paula Rodriguez-Villamil  Luciana Relly  Juliana Paula Martins Alves  Davide Rondina  José Luiz Rigo Rodrigues  Marcelo Bertolini 《Reproduction in domestic animals》2021,56(6):857-863

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胰岛素样生长因子—Ⅰ(IGF—Ⅰ)基因及其产物的表达调节     

刘晓辉 《山西农业大学学报(自然科学版)》1993,(1)

本文介绍了 IGF—I 的基因及其表达,体内 IGF—I 产生如何受发育、各种激素和营养的调节,以及这些调节途径的性质。  相似文献   

不同饲养水平对生长肥育猪生产性能和血清类胰岛素生长因子-I的影响     

邓跃林  N.OKSBJERG 《华南农业大学学报》2002,(4)

试验采用 2× 2因子设计 ,即生长期自由采食或喂自由采食量的 6 0 % ,肥育期自由采食或喂自由采食量的70 % .结果表明生长期限制饲养引起的生长受阻 ,在肥育期通过充分饲喂可以得到部分补偿 .在生长期 ,限制饲喂使猪血清中IGF I水平降低 2 9 4 % (P <0 0 1) ;而肥育期限制饲喂则对血清中IGF I含量没有影响 .与自由采食的猪血清相比 ,生长期限制采食的猪血清 ,可使C2 C12 细胞的增殖能力降低 18 8% (P <0 0 1) ;肥育期限制采食的猪血清 ,则使蛋白质的合成降低 11 5 % (P <0 0 1) ,使蛋白质的降解增加 5 5 % .试验结果说明肥育期的补偿生长与血清中IGF I水平无关 ,可能与血清中其他生长因子的增多有关 .  相似文献   

Development of a protein binding assay for teleost insulin-like growth factor (IGF)-like: relationships between growth hormone (GH) and IGF-like in the blood of rainbow trout (Oncorhynchus mykiss)     

Ping-De Niu  Jaime Perez-Sanchez  Pierre-Yves Le Bail 《Fish physiology and biochemistry》1993,11(1-6):381-391

Using rainbow trout plasma protein (IGF-BP) which specifically binds human insulin-like growth factor (IGF) (Niu and Le Bail 1993), we have developed an assay to measure plasma IGF-like levels in different teleost species. Before the assay and to prevent interference by IGF-BP, IGF-like was extracted from all samples, using SP Sephadex C-25 in acidic conditions. After this treatment, contamination of the IGF fraction by IGF-BP which was estimated by binding assay, was approximately 5%, and was not detectable by western ligand blot. Human IGF-I was used as standard and labelled hormone. Sensitivity of the assay was 0.15–0.40 ng/ml (ED90) and ED50 was 1–3 ng/ml. hIGF-II crossreaction was partial and no significant displacement was observed with Insulin from different species or with other hormones. Inhibition curves were obtained with plasma IGF fractions (but not with tissue extracts) from teleost and mammals and are parallel to the standard curve. These results suggest that the protein binding assay can quantify an IGF-like factor in the plasma of teleost and that the binding sites of IGF are well conserved during vertebrate evolution. Using this IGF binding assay, IGF-like was measured in parallel with growth hormone (GH) in plasma from young rainbow trout killed every 1.5h throughout one day. The daily profiles for both hormones, which appear pulsatile, are similar. A significant correlation was observed between GH levels and IGF-like levels with a 1.5h delay. Analogous observations were obtained in individual catheterized adult rainbow trout. Although plasma GH levels differ greatly between fish, less variability is found with IGF-like. In a third experiment, rainbow trout were starved or submitted to bovine GH treatment for four weeks. Starved fish, in which plasma GH levels increased, had plasma IGF-like level significantly lower than in fed fish. In bGH injected fish, plasma IGF-like level was significantly higher than in non-injected fish. These results suggest that, as in mammals, IGF-like secretion depends on plasma GH level and could be modulated by the nutritional status of fish.  相似文献   

IGF1-PI3K-Akt信号通路相关基因在黄羽肉鸡肌肉和肝脏中的表达     

束婧婷  姬改革  单艳菊  章明  巨晓军  刘一帆  屠云洁  盛中伟  唐燕飞  蒋华莲  邹剑敏 《中国农业科学》2021,54(9):2027-2038

【目的】IGF1-PI3K-Akt信号通路是调控生长发育的关键通路,选择慢速型的广西麻鸡和中速型的花山麻鸡作为研究对象,探究IGF1-PI3K-AKT信号通路相关基因胰岛素样生长因子1( Insulin-like growth factors, IGF1)、胰岛素样生长因子1受体( Insulin- like growth factor receptor, IGF1R)和胰岛素受体底物1 (Insulin receptor substrate1, IRS1)在骨骼肌和肝脏中的发育性表达规律,为阐明黄羽肉鸡生长发育调控机理提供参考。【方法】采用SPSS20.0比较广西麻鸡和花山麻鸡在胚胎期至出生后早期的生长发育差异,采用实时荧光定量PCR方法检测上述3个基因在两个品种鸡胚胎期（9、12、16胚龄）和出生后（0、7、21、35、49和63日龄）胸肌、腿肌和肝脏中的表达差异,并将其与体重和组织重进行相关性分析。【结果】鸡生长发育早期体重、骨骼肌重和肝脏重变化呈现显著的品种和时间特异性,经过选育的花山麻鸡体重和组织重的增长在胚胎发育后期已经远远超过广西麻鸡。IGF1、IGF1R和IRS1基因表达存在显著的品种、组织和发育时段特异性,总体上,在胚胎期肌肉中的表达量高于肝脏组织,出生后则为肝脏中的表达量高于肌肉组织,广西麻鸡肌肉中的表达量高于花山麻鸡,而花山麻鸡肝脏中的表达量要高于广西麻鸡。IGF1基因在两个品种胸腿肌中均在9胚龄即可检测到表达,腿肌中表达量均是9胚龄时最高,除与12胚龄差异不显著外,与其他各阶段差异显著,表达量最低点出现在出雏0日龄时;胸肌中则是12胚龄时表达量最高,63日龄时最低;肝脏中则出现品种差异,广西麻鸡胚胎期未检测到表达,从出雏0日龄开始表达,表达高峰出现在21日龄时,而在花山麻鸡中虽然胚胎期检测到表达但表达量极低,49日龄时达到表达高峰。两品种鸡肌肉和肝脏各个阶段均检测到IGF1R mRNA的表达,在胸肌和腿肌中的表达量均表现为9胚龄最高,广西麻鸡9胚龄与其他各阶段差异显著,花山麻鸡9胚龄除与12胚龄差异不显著外,与其他各阶段均差异显著;肝脏中,广西麻鸡表达高峰出现在21日龄,且与9、12、16胚龄和0日龄差异显著,花山麻鸡则是63日龄时最高,与其他各阶段差异显著。两品种鸡肌肉和肝脏各个阶段均能检测到IRS1 mRNA的表达,在两品种肌肉中的表达量均表现为9胚龄或者12胚龄最高,与其他各阶段差异显著,随后下降,并在出雏后维持在较低水平;肝脏中IRS1的表达模式与IGF1R一致。IGF1、IGF1R和IRS1基因的表达两两之间均表现出显著或极显著的正相关关系,同时,肌肉中3个基因的表达与体重、胸（腿）肌重存在显著的负相关,而肝脏中3个基因的表达与体重、胸（腿）肌重、肝脏重均存在显著的正相关。【结论】IGF1-PI3K-AKT信号通路相关基因之间存在一致性趋势,品种间和组织间表达量的差异可能正是不同类型黄羽肉鸡生长发育差异的主要原因之一。  相似文献   

Decorin activates Akt downstream of IGF‐IR and promotes myoblast differentiation     

Keisuke Suzuki  Yasuhiro Kishioka  Jun‐ichi Wakamatsu  Takanori Nishimura 《Animal Science Journal》2013,84(9):669-674

Decorin, a small leucine‐rich proteoglycan, plays an important role in cellular activities through modification of growth factors. It also acts as a signaling molecule to non‐muscle cells through epidermal growth factor receptor or insulin‐like growth factor I receptor (IGF‐IR). However, it is unclear if decorin acts as a signaling molecule to myogenic cells. In this study, we investigated the effect of decorin on the differentiation of myoblasts and the signaling via IGF‐IR to myogenic cells. C2C12 myoblasts cultured in media containing decorin for 72 h showed more extensive formation of multinucleated myotubes than control cells cultured in the same media without decorin. The protein expressions of myogenin and myosin heavy chian were higher in decorn‐treated cells than in control cells. These results suggest that decorin enhances the differentiation of myoblasts. Western blot analysis and immunocytochemistry showed that IGF‐IR was expressed in myoblasts and myotubes. Furthermore, Akt, which is downstream of IGF‐IR, was more phosphorylated in myoblasts cultured in media containing decorin than those in media without decorin. These results suggest that decorin activates Akt downstream of IGF‐IR and enhances the differentiation of myogenic cells.  相似文献   

草原红牛IGF2基因外显子4的遗传多态性及遗传效应分析     

牛伟萍  刘晶  张金玉  张明军  杨润军  赵志辉 《吉林农业大学学报》2011,33(3):324-326,331

运用PCR-SSCP方法检测草原红牛IGF2基因第4外显子的多态性,采用最小二乘法拟合线性模型将突变位点的不同基因型与牛胴体和肉质性状进行关联分析.结果表明:在外显子4上有1个SNP位点(C250G),该基因具有AA和AB 2种基因型,草原红牛AB基因型个体肋脂质量比AA基因型个体高3.1%(P＜0.05),骨质量和眼...  相似文献   

温度与光照周期对公子小丑鱼幼鱼生长及相关生长基因表达的影响     

李泽滨  李云  蔡生力  鞠晨曦 《上海海洋大学学报》2017,26(3):321-329

探究了不同温度、光照周期对公子小丑鱼(Amphiprion ocellaris)幼鱼成活率、饵料转化率及生长率的影响,并利用实时荧光定量PCR技术检测了50 d后幼鱼肝脏生长激素受体基因Ⅰ型(Growth hormone receptor1)、生长激素受体基因Ⅱ型(Growth hormone receptor 2)、类胰岛素生长因子基因(Insulin-like growth factor)和类胰岛素生长因子结合蛋白基因(Insulin-like growth factor-binding protein)相对表达量的变化情况。结果显示,不同温度、光照周期对公子小丑鱼幼鱼的成活率无显著影响(P0.05),但对其生长率、饵料转化率影响显著。幼鱼的生长率、饵料转化率在温度27℃时最大,22℃次之,17℃、32℃较低;光照周期方面,幼鱼的生长率、饵料转化率在24L∶0D组最大,且显著高于其他光照周期组。荧光定量PCR的结果显示,幼鱼肝脏各生长相关基因的相对表达量在温度27℃时最高,且显著高于其他温度组(P0.05);光照周期方面,各基因在24L∶0D时相对表达量最高,且显著高于其他光照周期组(P0.05)。研究结果表明,公子小丑鱼幼鱼生长的最适温度为27℃,最适光照周期为24L∶0D。  相似文献   

杜大长猪IGF-I基因的克隆及其序列分析   总被引：5，自引：0，他引：5  

奚刚  许梓荣  戚益军 《中国兽医学报》2000,20(1):74-76

采用ＴＲＩzol法从杜大长杂交猪的肝脏中提出总ＲＮＡ,将其纯化后作为ＰＣＲ扩增模板 ,以设计的Ｐ１（５′－ＣＴＡＣＡＴＴＣＴＧＴＡＧＴＴＣＴＴＧＴＴＴＣＣ－３′）为引物合成ＩＧＦ－Ｉ基因cＤＮＡ的第１链,再以Ｐ１和Ｐ２（５′－ＡＴＧＧＣＣＣＴＧＴＧＣＴＴＧＣＴＣＴＣＣＴＴ－３′）为引物扩增到大小约为３６０bp的产和,并将其达隆至pＧＥＭ－Ｔ载体上。经筛选、酶切、我分析,表明该片段为ＩＧＦ－Ｉ基因的cＤＮＡ  相似文献