Factors affecting the transfer of immunoglobulin G1 into the milk of Holstein cows   总被引：2，自引：0，他引：2  

Liu GL  Wang JQ  Bu DP  Cheng JB  Zhang CG  Wei HY  Zhou LY  Zhou ZF  Hu H  Dong XL 《Veterinary journal (London, England : 1997)》2009,182(1):79-85

Immunoglobulin (Ig) G1 concentrations in milk from Holstein cows was measured to determine if transfer and concentration was influenced by production factors (lactation number, stage of lactation, daily milk production), milk composition (milk fat, protein, lactose, and total solids content) or by serum IgG1 concentration. Two hundred and ninety-nine Chinese Holstein cows were randomly selected from four herds containing a total of more than 1600 lactating animals. The concentration of IgG1 in the milk and serum was determined by ELISA.Milk IgG1 concentrations varied between 0.030 and 0.614 mg/mL and significantly correlated with lactation number, stage of lactation, daily milk production and somatic cell count. The IgG1 mass was found to highly correlate with lactation number, stage of lactation, daily milk production and milk protein content. Lactation number had the highest positive direct relationship with IgG1 concentration.  相似文献   

Evaluation of a commercially available enzyme-linked immunosorbent assay for the detection of allergen-specific IgE antibodies in dogs     

Saevik BK  Ulstein TL  Larsen HJ 《Research in veterinary science》2003,74(1):37-45

Twenty-eight atopic dogs, 22 pruritic, non-atopic dogs and 10 healthy dogs were ELISA tested. For calculations of diagnostic specificity and sensitivity, positive ELISA test results in non-atopic dogs were considered false positive results. The absence of any positive results in the atopic dogs was considered false negative results. The atopic dogs were tested both with ELISA and an intradermal test, utilising allergen extracts from the same manufacturer, to determine the frequency of positive allergen reactions in the ELISA test compared with the intradermal test. The Prausnitz-Küstner test was performed to evaluate the significance of a positive ELISA test result. Based on cross-tabulations with clinically defined atopic dermatitis, the ELISA test showed a sensitivity of 53.6% and a specificity of 84.4%. The correlation between the ELISA and the intradermal test was poor. Positive Prausnitz-Küstner tests were not obtained using sera from dogs that were intradermal test negative for the tested allergens, even though sera had high levels of IgE as measured by the ELISA. These findings question the significance of a positive ELISA test result and indicate that the test is not measuring functional allergen-specific IgE.  相似文献   

Polymorphisms in IL-4 and IL-4R genes in children with allergic asthma     

CUI Tian-pen  HU Li-hua  PAN Shi-xiu  XI Dong  WU Jian-min 《园艺学报》2005,21(1):125-128

AIM: To determine whether interleukin 4 and interleukin 4 receptor α chain are associated with allergic asthma in children and to study the impact of such polymorphism upon plasma IgE.METHODS: Two polymorphism sites of IL-4 and IL-4R were studied by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).RESULTS: (1)The results showed that the IL-4 promoter -589 was not associated with children allergic asthma, however, the IL-4R α chain 576RR genotype and R allele were significantly increased in the subjects with asthma in children compared with age-matched control subjects (χ2=11.84, P＜0.01; χ2=13.03, P＜0.01). The IL-4R α chain 576RR genotype was associated with higher plasma IgE. CONCLUSION: These date suggest that the IL-4R α chain R576 is a risk factor of allergic asthma in children in Chinese, and is also related with higher plasma IgE level.  相似文献   

Idiopathic hypereosinophilic syndrome in 3 Rottweilers     

Sykes JE  Weiss DJ  Buoen LC  Blauvelt MM  Hayden DW 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2001,15(2):162-166

Three Rottweilers with marked peripheral eosinophilia and infiltration of the liver, spleen, lungs, and bone marrow with eosinophils were diagnosed with idiopathic hypereosinophilic syndrome (IHES). Mean serum immunoglobulin E concentrations were markedly high. On cytogenetic analysis, no evidence of karyotypic abnormalities was found in bone marrow aspirates. Despite an extensive search, no underlying cause for the eosinophilia could be identified. In this study, cytogenetic analysis and measurement of serum IgE concentrations were used to differentiate IHES and eosinophilic leukemia.  相似文献   

Immunoglobulin M-capture enzyme-linked immunosorbent assay testing of cerebrospinal fluid and serum from horses exposed to west nile virus by vaccination or natural infection     

Porter MB  Long M  Gosche DG  Schott HM  Hines MT  Rossano M  Sellon DC 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2004,18(6):866-870

The West Nile (WN) virus, present in the United States since 1999, is a cause of encephalomyelitis in birds, alligators, humans, and horses. No data exist regarding detection of anti-WN virus immunoglobins in equine cerebrospinal fluid (CSF). The aims of this study were to evaluate the blood-brain barrier (BBB) in WN virus-infected (WNE) horses, to compare diagnostic testing in serum and CSF, and to describe the immunoglobulin M (IgM) response in serum and CSF of vaccinated horses. CSF was collected from the lumbosacral (LS) space (n = 13) or the allanto-occipital (AO) space (n = 14) of WNE horses. The albumin quotient (AQ) and IgG index were calculated, and the IgM-capture-enzyme-linked immunosorbent assay (MAC-ELISA) was used to detect anti-WN virus IgM in serum and CSF. CSF collected from the LS site had a higher (P < .02) IgG index compared to the AO site (0.34 +/- 0.04 versus 0.22 +/- 0.04 [mean +/- SE], respectively). The mean AQ, irrespective of collection site, did not exceed reference values. There was 100% agreement between CSF and serum testing for IgM by MAC-ELISA testing. However, the positive to negative antigen ratios were higher (P < .001) in CSF (34.5) versus serum (8.5), indicating lower nonspecific reactivity in CSF samples. Horses vaccinated against WN virus did not develop an IgM response at 1:400 mg/dL in serum; however, a few horses developed a weak IgM response in serum but not in CSF. In conclusion, MAC-ELISA testing of serum and CSF were equivocal. Also, examination of CSF data from WNE horses suggests a normal BBB integrity and increased intrathecal production of antibodies.  相似文献   

鸡贫血因子病的免疫病理学变化   总被引：6，自引：2，他引：6  

刘忠贵  郑世民 《中国农业科学》1997,30(4):74-82

 用鸡贫血病毒（CAV）感染1日龄健康AA雏鸡，以未感染同龄雏鸡为对照，在感染后7、14、21、28、35、42和49d检测其免疫器官与体重比值，胸腺、脾脏T细胞白细胞介素2（IL-2）和干扰素（IFN）诱生活性，胸腺、法氏囊、脾脏T、B细胞增殖反应，胸腺、法氏囊、脾脏、盲肠扁桃体及哈德尔腺中α-萘酚酯酶阳性[ANAE#+（+）]T细胞、IgG、IgM、IgA抗体生成细胞数量，外周血液中T、B细胞数量，血清、泪液、气管液、肠液、胆汁中IgG、IgM、IgA含量的动态变化。揭示了感染鸡细胞因子IL-2、IFN的免疫调节发生障碍、中枢与外周免疫器官的细胞免疫和体液免疫功能呈现抑制，呼吸道与消化道的局部免疫防御功能降低。  相似文献   

A mouse model of upper respiratory tract mucosal immunity dysfunction induced by cold stimulation     

LI Xian  LEI Na  DUAN Xiao-hua  WU Sheng-you  LI Xiu-fang  LIN Qing 《园艺学报》2011,27(8):1662-1664

AIM: To establish a mouse model of upper respiratory tract mucosal immunity dysfunction induced by cold stimulation. METHODS: Mice were stimulated with cold by placing the animals in-20 ℃ environment for 5 min, 10 min, 15 min or 20 min. The secretory immunoglobulin A(SIgA) level and lysozyme activity in the mouse saliva were determined for qualification. RESULTS: The mortality rate was 50% in the group of cold stimulation for 20 min. Compared with the control mice, the SIgA level and lysozyme activity significantly decreased in the group of cold stimulation for 15 min (P<0.05 and P<0.01,respectively). Decreased lysozyme activity was only observed in the group of cold stimulation for 10 min (P<0.05). The data in the group of cold stimulation for 5 min were almost similar to those in control group. CONCLUSION: A mouse model of upper respiratory tract mucosal immunity dysfunction was successfully established by cold stimulation.  相似文献   

Cloning and characterization of cDNAs encoding four different canine immunoglobulin γ chains     

Liang Tang  Craig Sampson  Matthew J. Dreitz  Catherine McCall 《Veterinary immunology and immunopathology》2001,80(3-4):259-270

cDNAs encoding four different canine immunoglobulin G (caIgG) γ chains were identified in this study. One of these IgG γ chain cDNAs, (caIgG-A), represents 92.5% of the IgG γ chain cDNAs in a dog spleen cell cDNA library; a second partial IgG γ chain cDNA (caIgG-B) was also identified in the library. The other two IgG γ chain cDNAs (caIgG-C and caIgG-D) were RT-PCR amplified from canine lymphoma samples. Comparison of the four different canine IgG γ chain cDNAs showed homologies from 83.6 to 89.2% and from 73.1 to 81.8% at nucleotide and amino acid sequence levels, respectively. Despite the high similarity in CH1, CH2 and CH3 domains among the different caIgG γ chains, the hinge regions were distinct, sharing only 19.0–35.2% homology at the amino acid level. No multiple duplication of the hinge region, as reported for human IgG1 and IgG3, was detected in any of the canine IgG γ chains. The numbers of cysteines in the putative hinge regions were found to be 3, 2, 7 and 3 for the four canine IgG heavy γ chains (A, B, C and D), respectively. Specific primers were designed based on caIgG γ chain hinge region DNA sequences and were used in RT-PCR for measuring different caIgG γ chain mRNA levels in canine PBMC samples.  相似文献   

Use of Fourier-transform infrared spectroscopy to quantify immunoglobulin G concentration and an analysis of the effect of signalment on levels in canine serum     

《Veterinary immunology and immunopathology》2015,163(1-2):8-15

Deficiency in immunoglobulin G (IgG) is associated with an increased susceptibility to infections in humans and animals, and changes in IgG levels occur in many disease states. In companion animals, failure of transfer of passive immunity is uncommonly diagnosed but mortality rates in puppies are high and more than 30% of these deaths are secondary to septicemia. Currently, radial immunodiffusion (RID) and enzyme-linked immunosorbent assays are the most commonly used methods for quantitative measurement of IgG in dogs. In this study, a Fourier-transform infrared spectroscopy (FTIR) assay for canine serum IgG was developed and compared to the RID assay as the reference standard. Basic signalment data and health status of the dogs were also analyzed to determine if they correlated with serum IgG concentrations based on RID results.Serum samples were collected from 207 dogs during routine hematological evaluation, and IgG concentrations determined by RID. The FTIR assay was developed using partial least squares regression analysis and its performance evaluated using RID assay as the reference test. The concordance correlation coefficient was 0.91 for the calibration model data set and 0.85 for the prediction set. A Bland–Altman plot showed a mean difference of −89 mg/dL and no systematic bias. The modified mean coefficient of variation (CV) for RID was 6.67%, and for FTIR was 18.76%.The mean serum IgG concentration using RID was 1943 ± 880 mg/dL based on the 193 dogs with complete signalment and health data. When age class, gender, breed size and disease status were analyzed by multivariable ANOVA, dogs <2 years of age (p = 0.0004) and those classified as diseased (p = 0.03) were found to have significantly lower IgG concentrations than older and healthy dogs, respectively.  相似文献   

Purification of secretory immunoglobulin A from milk of the brushtail possum (Trichosurus vulpecula)     

EE Doolin  RG Midwinter  AR McCarthy  BM Buddle 《New Zealand veterinary journal》2013,61(5):181-186

AIM: To identify and purify secretory immunoglobulin A (sIgA), a key effecter molecule in mucosal immune responses, from milk of the brushtail possum (Trichosurus vulpecula).

METHODS: Milk samples were collected from female possums with pouch young, and clarified by centrifugation and precipitation methods. The clarified fraction was purified by gel filtration and affinity chromatography to yield sIgA. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting techniques were used to assess the purity of the final product, and to identify the heavy (H) chain, light (L) chain and secretory component (SC) of possum sIgA.

RESULTS: Immunoblotting, using antibodies raised against cloned possum sIgA SC and H-chain, and a synthetic peptide fragment of the H-chain, confirmed the identity of the purified protein. The N-terminal amino acid sequence of purified possum sIgA showed strong homology to reported sequences of H-chain variable regions of marsupial immunoglobulins.

CONCLUSIONS: Milk was shown to be a convenient source of mucosal secretion containing sIgA, and a process involving 2 precipitation and 2 chromatography steps produced purified sIgA. This IgA preparation will prove useful for the generation of sIgA-specific immunological reagents for measurement of immune responses in the development of mucosal-based vaccines for biological control of possums.  相似文献