Cytokine and immunoglobulin isotype profiles during CP7_E2alf vaccination against a challenge with the highly virulent Koslov strain of classical swine fever virus     

P. Renson  M. Le Dimna  C. Gabriel  R. Levai  S. Blome  G. Kulcsar  F. Koenen  M.F. Le Potier 《Research in veterinary science》2014

CP7_E2alf is a promising marker vaccine candidate against classical swine fever (CSF). To better understand the mechanisms of protection, cytokine and isotype-specific antibody profiles were investigated in CP7_E2alf vaccinated pigs before and after challenge with the highly virulent CSFV strain “Koslov” at 14 days or 6 months post-vaccination. The interference of vaccination with CSFV pathogeny-related cytokine responses, previously described following a moderately virulent challenge, was confirmed. However, the levels of additional cytokines, TNF-α and IL-6, were significantly attenuated by vaccination following highly virulent challenge. This vaccine interference with cytokine response was not dependent on the immunization route or the consequence of competition between vaccine and challenge strain. Interestingly, IFN-γ enhancement and persistent high IgG2 levels suggested an important role of cell-mediated immunity in long-term protection against CSFV induced by CP7_E2alf vaccination. IgA production also revealed a stimulation of mucosal immunity, especially after oral administration of the vaccine.  相似文献   

Serological status of mares in parturition and the levels of antibodies (IgG) against protozoan family Sarcocystidae from their pre colostral foals     

Felipe Lamberti Pivoto  Arlindo Gomes de Macêdo Junior  Murilo Vieira da Silva  Flávia Batista Ferreira  Deise Aparecida Oliveira Silva  Endrigo Pompermayer  Luis Antônio Sangioni  Tiago Wilson Patriarca Mineo  Fernanda Silveira Flores Vogel 《Veterinary parasitology》2014,199(1-2):107-111

Protozoa from the family Sarcocystidae are agents of reproductive and neurological disorders in horses. The transmission of these protozoa may occur via horizontal or vertical means, and the frequency and potential of the later is not fully elucidated in horses. Thus, the aim of study was to correlation levels of antibodies in mares with pre colostral foals seropositive and assess the level and distribuition of antibodies against Neospora spp., Sarcocystis neurona and Toxoplasma gondii, in mares and pre colostral foals at the parturition. The blood samples were collected from mares immediately after parturition and from newborns before the ingestion of colostrum, and sera were analyzed for the presence of IgG by ELISA. It was found that 21.5%, 33.7% and 27.6% of mares were seropositive for Neospora spp., S. neurona and T. gondii, respectively; foals had antibodies at a rate of 8.3%, 6.6% and 6.6% for Neospora spp., S. neurona and T. gondii, respectively. Additionally, paired samples from mares and pre-colostral foals revealed an overall negative correlation between the serum reactivity against these three parasites and suggested that seronegative mares, or those with low to intermediate antibody levels, have a higher risk of giving birth to seropositive foals.  相似文献   

Usefulness of a commercial equine IgG test and serum protein concentration as indicators of failure of transfer of passive immunity in hospitalized foals     

Metzger N  Hinchcliff KW  Hardy J  Schwarzwald CC  Wittum T 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2006,20(2):382-387

Detection of failure of transfer of passive immunity (FTPI) is important in reducing morbidity and mortality in neonatal foals. We investigated the performance of a commercial equine IgG test (SNAP Foal IgG Test Kit) to diagnose FTPI in hospitalized foals. Furthermore, we evaluated the usefulness of serum total protein (STP) and serum globulin (SG) concentrations as indicators of FTPI. Serum IgG concentration was measured by means of the SNAP test and single radial immunodiffusion, and SG and STP concentrations were determined by means of a clinical chemistry analyzer. Subjects were 67 hospitalized foals <19 days old. The SNAP test was repeated on 37 samples from 29 foals, with identical results for 24 samples (kappa statistic, 0.64; 95% confidence interval [CI], 0.46-0.82). The sensitivity of the SNAP test to detect serum IgG concentration [IgG] < or =400 and < or =800 mg/dl was 90% (95% CI, 71-98%) and 95% (85-99%), respectively, and the specificity was 79% (71-82%) and 52% (39-57%), respectively. Sensitivity for detection of [IgG] < or =400 mg/dl was not affected (P > .05) by plasma fibrinogen concentration, sepsis score, or bacteremia. Specificity for detection of [IgG] < or = 800 mg/dl was lower (P < .05) in foals with sepsis score < or =11 (50% [31-60%] versus 100% [8-100%]) and bacteremia (25% [5-56%] versus 62% [45-62%]). Sensitivity and specificity of [STP] < or = 5.0 g/dl for [IgG] < or =800 mg/dl was 94% (83-99%) and 47% (30-56%), respectively. Performance of the SNAP test in hospitalized foals is impaired because of low specificity, but can have usefulness provided that the properties of the test and characteristics of the foal being examined are considered when interpreting the results. The STP and SG concentrations are poor sole indicators of FTPI in hospitalized foals, but may be useful adjunctive tests.  相似文献   

Passive transfer of naturally acquired specific immunity against West Nile Virus to foals in a semi-feral pony herd     

Wilkins PA  Glaser AL  McDonnell SM 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2006,20(4):1045-1047

Horses naturally exposed to West Nile Virus (WNV) or vaccinated against WNV develop humoral immunity thought to be protective against development of clinical disease in exposed or infected animals. No reports evaluate the efficacy of passive transfer of naturally acquired specific WNV humoral immunity from dam to foal. The purpose of this study was to investigate passive transfer of naturally acquired immunity to WNV to foals born in a herd of semi-feral ponies, not vaccinated against WNV, in an endemic area, with many dams having seroconverted because of natural exposure. Microwell serum neutralization titers against WNV were determined in all mares and foals. Serum IgG concentration was determined in foals by serial radial immunodiffusion. Differences in IgG concentration between seropositive and seronegative foals were examined by means of the Mann-Whitney U-test. Linear regression was used to evaluate the association between mare and foal titers. Seventeen mare-foal pairs were studied; 1 foal had inadequate IgG concentration. IgG concentration was not different between seronegative and seropositive foals (P = .24). Mare and foal titers were significantly correlated in foals with adequate passive transfer of immunity (Spearman's rho = .84; P < .001); >90% of the foal's titer was explained by the mare's titer (R2 = 0.91; P < .001). Passive transfer of specific immunity to WNV is present in pony foals with adequate passive transfer of immunity born to seroconverted mares.  相似文献   

Dynamics of Leishmania-specific immunoglobulin isotypes in dogs with clinical leishmaniasis before and after treatment     

Rodríguez A  Solano-Gallego L  Ojeda A  Quintana J  Riera C  Gállego M  Portús M  Alberola J 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2006,20(3):495-498

Concentrations of Leishmania-specific immunoglobulin G (IgG), immunoglobulin M (IgM), and immunoglobulin A (IgA) isotypes were analyzed by enzyme-linked immunosorbent assay (ELISA) in 23 dogs naturally infected with Leishmania infantum before and 1 year after initiating drug therapy. Results showed a high expression and prevalence of Leishmania-specific IgG (176.4 +/- 89 ELISA units [EU]), IgM (105.3 +/- 95.5 EU), and IgA (153.6 +/- 98 EU) in dogs before treatment (median +/- interquartile range EU). One year after treatment was started, dogs were classified as responsive dogs (RDs; n = 13) or unresponsive dogs (UDs; n = 10) based on clinicopathologic findings. Both groups of dogs experienced a statistically significant decrease (P < .05) in Leishmania-specific IgG (RDs = 27%, UDs = 41%), IgM (RDs = 42%, UDs = 29%), and IgA (RDs = 56%, UDs = 46%). Concentrations of specific IgG and IgM were not different at diagnosis or after treatment between the 2 groups. However, the median value for Leishmania-specific IgA 1 year after treatment was significantly lower (P < .05) in RDs (60.8 +/- 67 EU) than in UDs (117 +/- 54 EU). Examination of our data indicates that both the IgA isotype, which is mostly produced by mucosal plasma cells, and the IgM isotype are increased in infected symptomatic dogs, as previously reported for IgG. These 3 isotypes decreased significantly 1 year after initiation of medical treatment.  相似文献   

家禽卵黄抗体作用机理及应用现状分析   总被引：2，自引：0，他引：2  

燕海峰  邓缘  朱立军  肖兵南  P.Trefil 《畜禽业》2006,(18):28-31

卵黄抗体(IgY)来源廉价、特异性好、见效快,其作为一种理想的绿色饲料(食品)添加剂的应用正倍受关注。本文就其作用机理、产业化研究与应用现状、存在的问题进行了综述,并提出了建议,以加速其在畜牧养殖业和食品添加剂行业的推广应用。  相似文献   

肌注手足口病(EV71型)人免疫球蛋白层析工艺研究     

梁小明  周星  何淑琴  黄璠  刘敏亮 《安徽农业科学》2013,(31):12262-12265

[目的]优化手足口病（EV71型）人免疫球蛋白的层析工艺条件,为制备治疗手足口病（Ev71型）的人免疫球蛋白提供依据。[方法]采用单因素试验和响应面分析法,研究样品pH值、蛋白质浓度和电导率对层析后流穿液中免疫球蛋白纯度的影响。[结果]影响层析流穿液中免疫球蛋白纯度的主要因素为电导率,其次是蛋白质浓度,pH值对层析流穿液中免疫球蛋白蛋白纯度影响最小。手足口病（EV71型）人免疫球蛋白的最佳层析工艺参数为：pH5．28、蛋白质浓度41．62g／L、电导率为0．24m／s。[结论]层析优化后的流穿液中手足口病（EV71型）人免疫球蛋白纯度可达到99．60％,可用于制备高纯度的手足口病（EV71型）人免疫球蛋白。  相似文献   

Effects of immunoglobulin on circulating proinflammatory cytokines in children with hand-foot-mouth disease     

CHENG Hong-qiu  HUANG Cai-hua  LIU Wei-dong  YAO Zhan-cheng  YU Gui-qin 《园艺学报》2013,29(3):546-548

AIM：To study the mechanism of intravenous administration of immunoglobulin for treatment of hand-foot-mouth disease. METHODS：Sixty-four children with hand-foot-mouth disease were randomly divided into treatment group and control group. The children in control group (n=32) were treated with ribavirin and given normal supportive therapy. The children in treatment group (n=32) were given ribavirin and normal supportive therapy plus intravenous administration of immunoglobulin (500 mg·kg-1·d-1) for 3 d. The levels of TNF-α, C-reactive protein (CRP) and IL-6 in venous blood were observed. RESULTS：The levels of TNF-α, CRP and IL-6 in treatment group and control group were all higher than those in the normal children. The levels of TNF-α, CRP and IL-6 in treatment group were similar to those in control group before treatment. The levels of TNF-α, CRP and IL-6 in treatment group were significantly lower than those in control group after treatment with immunoglobulin for 3 d. CONCLUSION：The systemic inflammatory response syndrome is one of the possible mechanisms of hand-foot-mouth disease. Intravenous administration of immunoglobulin decreases the proinflammatory cytokines in the patients with hand-foot-mouth disease.  相似文献   

IgA₁ from patients with IgA nephropathy induced release of calcium,up-regulation of TGF-β mRNA expression and secretion of fibronectin in human glomerular mesangial cells     

WANG Yue  ZHAO Ming-hui  ZHANG You-kang  LI Xiao-mei  WANG Hai-yan 《园艺学报》2004,20(6):1025-1029

AIM: To study and compare the pathophysiological effects of serum IgA₁ from both the patients with IgA nephropathy (IgAN) and healthy controls on human mesangial cells (HMC). METHODS: Serum IgA₁ was isolated with Jacalin affinity chromatography and heated to form aggregates (aIgA₁). Primary HMC were cultured and passage 3 and passage 4 of the cells were used. Intracellular calcium release was assayed with confocal analysis. Expression of TGF-β mRNA and the content of supernatant fibronectin were tested by RT-PCR and indirect competitive ELISA, respectively. RESULTS: aIgA₁ from patients with IgAN was shown to induce release of intracellular calcium, up-regulation of expression of TGF-β mRNA and secretion of fibronectin in HMC in a similar time-dependent manner as aIgA₁ from healthy controls, but the effects of the former were much stronger and the durations was much longer (P<0.05, respectively). CONCLUSION: IgA₁ from patients with IgAN was shown to be able to stimulate HMC with a stronger biological effects than that from healthy controls. It is suggested that direct interaction between IgA₁ from patients with IgAN and HMC may be another mechanism in the pathogenesis of IgAN.  相似文献   

Evaluation of Digital and Optical Refractometers for Assessing Failure of Transfer of Passive Immunity in Dairy Calves          下载免费PDF全文

I. Elsohaby  J.T. McClure  G.P. Keefe 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2015,29(2):721-726

Background

Failure of transfer of passive immunity (FTPI) is the underlying predisposing risk factor for most early losses in dairy calves. Refractometers, either optical or digital, can be used to assess FTPI as a part of calf health monitoring program on dairy operations.

Objectives

To evaluate the performance of and differences between digital Brix and optical refractometers for assessing FTPI in dairy calves.

Animals

Two hundred Holstein calves from 1 to 11 days of age.

Methods

A cross‐sectional study was designed to measure serum IgG concentration by radial immunodiffusion (RID) assay, digital Brix and optical refractometers. The correlation coefficients (r) between the 2 refractometers were plotted against each other and against the measured IgG concentration from RID. The Se, Sp, and accuracy of digital Brix and optical refractometers for assessing FTPI using previously recommended cut‐offs were calculated. A receiver operating characteristic curve was created and used to identify the optimal cut‐off for this dataset.

Results

The RID IgG concentration was positively correlated with digital Brix (r = 0.79) and optical (r = 0.74) refractometers. The best combination of Se (85.5%), Sp (82.8%), and accuracy (83.5%) of digital Brix refractometer was at 8.3%Brix. For optical refractometer, the best combination of Se (80%), Sp (80.7%), and accuracy (80.5%) was at 5.5 g/dL.

Conclusions and Clinical Importance

Both refractometers exhibited utility in assessing FTPI in dairy calves.  相似文献