Mechanism of quercetin improving rat coronary artery myogenic response under high glucose     

HOU Xiao-min  ZHANG Ming-sheng  ZHAO Liang-yuan  QI Ding-ming  QIN Xiao-jiang 《园艺学报》2017,33(10):1801-1805

AIM: To investigate the mechanism of quercetin improving rat coronary artery myogenic response under high glucose (HG) by measuring muscle tension of coronary arterial ring and recording voltage-gated K⁺ channel (Kv) current of coronary artery smooth muscle cells by whole cell patch clamp. METHODS: The coronary rings from the normal SD rats were acutely isolated, and then divided into 6 groups: (1) control group; (2) HG group; (3) HG+low dose (3 μmol/L) of quercetin group; (4) HG+moderate dose (10 μmol/L) of quercetin group; (5) HG+high dose (30 μmol/L) of quercetin group; (6) HG+C6303 (PKC inhibitor)+high dose of quercetin group. Determinations of coronary artery response to vasoconstrictor (60 mmol/L KCl or 0.1 mmol/L U46619) or vasodilator (ACh at 10^-9~10^-5 mol/L) were performed, and the percentage of coronary ring tension was calculated using the contraction as 100% caused by 60 mmol/L KCl. The rat coronary artery smooth muscle cells were acutely isolated for recording the Kv current using whole cell patch clamp. RESULTS: Compared with control group, the contraction amplitudes to 60 mmol/L KCl or 0.1 mmol/L U46619 were significantly increased under HG incubation. Quercetin intervention concentration-dependently reduced the coronary artery contraction amplitude. Incubation of PKC specific inhibitor C6303 attenuated the effect of quercetin. Compared with control group, the diastolic amplitude to ACh decreased significantly in HG group, and quercetin intervention concentration-dependently increased the coronary artery diastolic amplitude. Incubation of PKC specific inhibitor C6303 attenuated the effect of quercetin. Compared with control group, HG incubation inhibited Kv current of coronary artery vascular smooth muscle cells significantly, and quercetin intervention attenuated the inhibitory effect of HG on Kv current intensity. Incubation of PKC specific inhibitor C6303 attenuated the effect of quercetin. CONCLUSION: Quercetin has a protective effect on myogenic response of coronary artery under HG and the effects is related to the increase in Kv current and the activation of PKC in vascular smooth muscle cells.  相似文献   

Model construction of rat coronary artery smooth muscle cell endoplasmic reticulum stress induced by thapsigargin     

CHEN Xiao-yan  DENG Chun-yu  KUANG Su-juan  YANG Hui  RAO Fang  SHAN Zhi-xin  LIN Qiu-xiong  JIANG Li 《园艺学报》2017,33(1):128-132

AIM: To investigate the primary culture method for coronary artery smooth muscle cells (CASMCs), and to establish the endoplasmic reticulum stress (ERS) model in CASMCs of SD rats. METHODS: CASMCs were cultured by tissue explant method. The morphological characteristics were observed under optical microscope. The marker proteins of CASMCs, including α-SMA and SM-MHC, were identified by immunofluorescence technique. The protein expression levels of BiP and CHOP, the marker molecules of ERS, were determined by Western blot. RESULTS: The spindle-shaped CASMCs climbed out from the edge of coronary artery tissues after 6 d, and formed the typical "hill and valley" growth pattern of CASMCs at 9~10 d. The result of immunofluorescence technique showed that α-SMA and SM-MHC were positively expressed. The results of Western blot showed that the protein expression of BiP and CHOP in TG (1 and 2 μmol/L) treatment groups was increased compared with control group. Compared with control group, the protein expression of BiP and CHOP was significantly increased after 1 μmol/L TG treatment for 24 and 48 h. CONCLUSION: CASMCs can be successfully cultured by tissue explant method. ERS model of CASMCs was established by 1 μmol/L TG treatment for 24 h.  相似文献   

秤锤树叶片内生真菌的分离鉴定及其对植株生长的影响     

李霞  曹昆  丛伟 《广西农业生物科学》2010,(1):75-81

本研究从秤锤树叶片中分离内生菌进行分子鉴定,并接种到无菌组培苗中研究其对植株生长的影响。研究结果显示,从秤锤树叶片中共分离获得7株真菌。扩增出的6个菌株经ITS测序,在NCBI网站上进行基因序列比对,鉴定出其中5株菌株均属于球毛壳菌（Chaetomium globosum）,1株属于子囊菌（As-comycete）。进一步实验表明：分离的菌株多数对组培苗的株高生长影响不大,可初步鉴定其为内生菌,其中的属于球毛壳菌的F和H菌株对无菌苗的株高有明显的促进作用。  相似文献   

冻融对粉质黏土强度指标的影响     

李洪峰 《东北林业大学学报》2012,40(6):108-110,136

依托同三高速公路扩建工程方正—哈尔滨段加宽一侧粉质黏土路基,采用室内三轴试验方法,研究了不同压实度、不同含水率下不同冻融次数后土体强度指标的变化规律。试验结果表明粉质黏土冻融后强度指标发生规律性变化:土的黏聚力损失随着冻融次数增多而增大,同一压实度下含水率高的土体黏聚力损失幅度较大;土的内摩擦角随着冻融次数的增多有逐渐增长的趋势,但增长幅度较小。运用Matlab对试验数据进行回归分析,建立了同一压实度下粉质黏土黏聚力、内摩擦角与含水率和冻融次数的回归方程。该方程能够直观模拟冻融对粉质黏土强度指标的影响规律,计算结果与实测结果吻合良好,可用于冻融后粉质黏土黏聚力和内摩擦角的计算。  相似文献   

高效液相色谱内标法测定斑蝥中斑蝥素的含量     

刘小松  王方方  国光梅  汪冶 《山地农业生物学报》2012,31(2):162-164

采用HPLC法,建立高效液相色谱内标法测定斑蝥中斑蝥素的含量。结果表明,斑蟊素在0.05～1.00mg/mL范围内线性良好(R=0.999 9),其平均回收率为97.74%。该方法简便、快速、准确、重复性好,可用于斑蝥中斑蝥素含量测定。  相似文献   

非洲鸵鸟冠状动脉的解剖观察   总被引：5，自引：0，他引：5  

肖传斌  刘忠虎  蒋媛媛  徐亚平  高春生  王平利  梁宏德 《河南农业大学学报》2005,39(2):218-221

采用血管铸型和组织透明方法对6羽非洲鸵鸟心脏冠状动脉的分支分布情况进行了研究,试验结果表明,非洲鸵鸟心脏左、右冠状动脉均开口于同侧主动脉窦内;左冠状动脉分为浅支(旋支)和深支(前降支),浅支先分出左心房支布于左心房,主干分支分布左心室的左缘壁和隔壁及右心室隔壁的部分;深支又依次分出动脉圆锥支、左缘支和前室间隔支,动脉圆锥支分布动脉圆锥及肺动脉干,左缘支分支分布于左室侧壁,前室间隔支分布于室间隔前1／3部,前降支主干分支分布于左心室前壁,右冠状动脉也分为浅支(旋支)和深支,浅支分出窦房结支和右心房支,主干延续为右室后支,窦房结支分布于窦房结和升主动脉,右心房支分布于右心房,右室后支分布于右心室的隔壁;深支分出后室间隔支,主干延续为右室前支,后室间隔支分布于室间隔后2／3部,右室前支分布于右心室前壁及心右缘壁。  相似文献   

农村受众的新闻媒介接触行为研究——基于MATLAB模拟方法     

康亢  贾殿华  李刚 《安徽农业科学》2016,(1):336-338

城镇化趋势下,农村受众利用媒介接触获取新闻呈多元化趋势。每个农村受众都是具有自智能性自决策性主体,其农村受众的媒介接触行为与自身内部属性存在联系。内部属性信息的异质化程度决定农村受众的媒介接触行为。该研究根据在云南省晋宁县的入户调查数据,提取农村受众的媒介接触行为与自身内部属性信息,基于MATLAB平台,通过模拟手段简化调查手段,生成模拟农村受众的媒介接触行为信息,通过回归分析得出,农村受众的媒介接触行为与自身内部属性相关,按影响程度大小依次为农业收入比例、年龄、耕地数量、家庭外出务工人数、信息需求度、受教育程度。  相似文献   

人和哺乳动物支气管动脉与肺动脉吻合现象研究进展     

杜晓霞  俞红贤  刘英  郭建强  赵建红  王宇  柳东阳  赵海涛 《动物医学进展》2007,28(5):46-49

对人和哺乳动物支气管动脉与肺动脉吻合问题的研究进行了概述.肺动脉与支气管动脉吻合的意义在于支气管动脉的高氧血液可以进入肺动脉代偿供应肺泡组织,肺动脉的营养血液也可以进入支气管动脉代偿供应肺间质.肺动脉与支气管动脉之间血管吻合常可以发生在胸膜面、支气管壁、终末细支气管或呼吸性细支气管等部位,也可以在毛细血管上发生支气管动脉与肺动脉的吻合.根据血管吻合的长度和口径,将其分为长吻合型和短吻合型,按照血管吻合的方式分为端-端吻合和端-侧吻合.在某些病理情况下,肺动脉与支气管动脉之间的吻合支还会通过管径扩张,增加血流量来供应局部的病变组织,因此有利于防御不利因素对机体造成的损伤,为机体实施正常的呼吸功能提供保障.  相似文献   

加热法对石膏刨花板性能影响的研究   总被引：1，自引：0，他引：1  

邓玉和  周梅  骆嘉言  雷文  张立方  张俊 《林产工业》2006,33(5):22-24

研究了加热温度和加压时间对石膏刨花板性能的影响。试验结果表明石膏固化时加适量的热量有助于提高石膏刨花板的性能;加热温度较高时石膏刨花板的性能比常温条件下压制的板的性能低。石膏固化时是吸热反应,石膏刨花板加压固化时,外加一定的热量有助于提高石膏的固化强度,因此板具有较好的物理力学性能。  相似文献   

DOPPLER SONOGRAPHIC EVALUATION OF THE DIGITAL BLOOD FLOW IN HORSES WITH LAMINITIS OR SEPTIC PODODERMATITIS     

SANTI WONGAUMNUAYKUL  CLAUDIA SIEDLER  HERMANN SCHOBESBERGER  CHRISTIAN STANEK 《Veterinary radiology & ultrasound》2006,47(2):199-205

The aim of this study was to evaluate the use of Doppler ultrasonography to monitor vascular blood flow dynamics in defined diseases of the equine digit in a noninvasive way. Doppler sonography was used to evaluate medial digital artery blood flow in eight horses with septic pododermatitis and four horses with laminitis in comparison with 10 horses of a control group. Doppler sonographic measurement and lameness examinations were performed in lame horses before treatment (day 0) and at 3, 6, and 9 days following treatment. Before treatment, blood flow velocities, arterial diameter, and flow volume were significantly higher in the lame horses, while pulsatility indices (PIs) were significantly lower (P < 0.05). Compared with the control group, there was no significant difference of resistive indices (RI) in the septic pododermatitis group, but these indices were significantly lower in the laminitis group. No significant difference between the two lame groups was recorded. After treatment, blood flow velocities and flow volume decreased significantly and PIs increased significantly in both lame groups, while RI and arterial diameter did not change. No correlation between Doppler parameters and the degree of lameness was found. Doppler sonography can be used to monitor vascular blood flow dynamics in horses suffering from septic pododermatitis and laminitis, while blood flow velocities and PI can serve as parameters for monitoring the course of the disease.  相似文献