克氏肺炎杆菌AS1.1736菌株培养条件研究     

管艺飞  郑艳  刘长江 《西北农业学报》2006,15(4):80-82,125

克雷伯氏肺炎杆菌（Klebsiella）AS1．1736具有较高的1，3-丙二醇转化率及底物浓度耐受性。从培养环境、培养时间及培养温度等因素对发酵的影响方面研究了克氏AS1．1736菌株的产菌量培养条件，结果表明，克氏肺炎杆菌AS1．1736菌株在32℃好氧环境下培养18h可获得较高的产菌量。  相似文献   

鸡肺炎克雷伯氏菌的分离鉴定   总被引：4，自引：1，他引：4  

彭远义  刘华英  吕寿英  王豪举  俞纯方 《西南大学学报(自然科学版)》1995,17(2):165-167

从自然病死鸡体内分离到一种革兰氏阴性的粗短杆菌，大小约０．５～０．９μｍ×０．８～１．６μｍ，两端顿圆或略尖，单个散在或两两相连，外有英膜包围，在麦康凯琼脂培养基上形成粘稠性粉红色菌落，不运动，不产生芽跑，经细菌学鉴定为肺炎克雷伯氏菌。该菌可致死小白鼠，对庆大霉素等药物敏感。  相似文献   

鲤鱼肺炎克雷伯氏菌分离与鉴定   总被引：2，自引：0，他引：2  

卢玉婷 《中国兽药杂志》2014,48(9):9-13

初步了解肺炎克雷伯氏菌在鲤鱼鱼体中的发病机制并筛选防治药物,为预防和治疗该病提供准确实验数据。对发病鲤鱼进行细菌分离纯化,通过革兰氏染色反应,生理生化反应测定单菌落特性,进行细菌回归试验和药敏试验。对注射菌液后发病的鱼进行细菌再分离,得到革兰氏阴性菌,并且与七叶苷及其它17种指标反应呈阳性,与戊二酰-甘氨酸-精氨酸-AMC及其它11种指标反应呈阴性,注射该菌液48 h后鱼体出现与疑似病例相同病症。分离得到的细菌,按《伯杰氏细菌鉴定手册》进行鉴定,确定为肺炎克雷伯氏菌。药敏试验结果表明该菌对亚胺培南等6种药物高度敏感。本试验首次从鲤鱼体内分离出肺炎克雷伯氏菌,并得到6种高度敏感的药物,同时阐明了肺炎克雷伯氏菌在鲤鱼中发病症状。  相似文献   

Integrated approach of metal removal and bioprecipitation followed by fungal degradation of organic pollutants from contaminated soils     

F. Baldi  V. Leonardi  A. D'Annibale  A. Piccolo  F. Zecchini  M. Petruccioli 《European Journal of Soil Biology》2007,43(5-6):380

This study reports the assessment of a novel process combining sequential treatments of a historically contaminated soil from the ACNA site (Cengio, Savona, Italy), a decommissioned industrial area. The soil was leached with 0.5 M citric acid leading to a removal of metals in the following order: Pb (74.2%) > Cu (72.6%) > Zn (40.2%) > Ni (55.7%) > Cd (41.5%) > Cr > (21.7%) Co > (19%) Fe (8.2%) with a concomitant low removal of organic contaminants (12%). The leachate was then incubated with the metal-resistant Klebsiella oxytoca strain BAS-10, capable of using residual citrate to produce an iron gel that co-precipitated metals. Concomitantly, the leached solid waste was bioaugmented with the autochthonous isolate of Allescheriella sp. DABAC 1 leading to a complete degradation of several organic contaminants, including trichlorobenzene, naphtalene, dichloroaniline and pentachloroaniline. The overall removals of organic contaminants by the fungus were 44.3% and 63.8% in non-leached and leached soil.  相似文献   

Using the spermosphere model technique to describe the dominant nitrogen-fixing microflora associated with wetland rice in two Egyptian soils     

A. M. N. Omar  C. Richard  P. Weinhard  J. Balandreau 《Biology and Fertility of Soils》1989,7(2):158-163

Summary This study is an attempt to describe the dominant N₂-fixing microflora associated with the roots of wetland rice. Rice cultivar Giza 171 was grown in a phytotron on two alluvial Egyptian soils for 8 days, a stage when the nitrogenase activity of undisturbed plants reached a level of 245 × 10^–6 mol C₂H₄ h^–1 g^–1 dry weight of leaf. The roots and rhizosphere soils were then used for counting and isolating dominant diazotrophs. Counts and initial enrichment steps were carried out on a selective medium made of an axenic rice plantlet, the spermosphere model, incubated under 1 % acetylene. The counts were very high, exceeding 10⁸ bacteria g^–1 dry weight of rhizosphere soil. Enterobacteriaceae were dominant; most isolates were Enterobacter cloacae belonging to different biotypes in the two soils. Enterobacter agglomerans, Citrobacter freundii and Klebsiella planticola were also present as members of the dominant microflora. Azospirillum brasilense and Azospirillum lipoferum were present as well, but less abundant.  相似文献   

Nitrogenase activity associated with leaf,root, and rhizosphere soils in an Indian tropical forest     

N. Gajendiran  A. Mahadevan 《Biology and Fertility of Soils》1989,8(1):71-74

Summary N₂ fixation (acetylene reduction assay) by phylloplane microorganisms was measured in dominant and co-dominant plant species growing in a tropical rain forest. No significant acetylene reduction was recorded with intact leaf samples. Azotobacter sp., Beijerinckia sp., Derxia sp., and Klebsiella pneumoniae were isolated as phylloplane N₂-fixing bacteria. Azospirillum lipoferum was only isolated from soil samples containing the roots of Poaceae. Nitrogenase activity was recorded in culture derived from the roots and rhizosphere soil samples, although low acetylene reduction activity indicates that these associations did not provide large amounts of N to the systems studied.  相似文献   

Characterization of bla CTX-M Gene in One Klebsiella pneumoniae Isolate from Sick Chickens in China     

HU Gong-zheng  HU Han  LIU Bao-guang  YUAN Li  LIU Jian-hua  PAN Yu-shan  WU Hua  CHEN Yu-xia 《中国农业科学(英文版)》2012,(10):1714-1720

  相似文献   

甘肃地区绵羊支原体病原菌的分离培养     

朱琳  常卫华  张勇  叶得河  赵兴绪 《草业科学》2013,30(9):1428-1432

在甘肃省5个地区采集了100份绵羊血清样本,并对100份样品进行了血清学及病原菌分离培养试验。血清学检测初步鉴定为14份阳性,其中11份来自于同一地区。然后对两例疑似患病羊做病原分离鉴定以及微生物学诊断和PCR鉴定,并对病原菌进行革兰氏染色。本研究成功地从病羊身体中分离出细小、纤细的呈球形、双球形、线状、螺旋状、半月状和短杆状等形态的绵羊肺炎支原体。  相似文献   

蟒蛇肺炎克雷伯氏菌的分离与鉴定          下载免费PDF全文

郭桂英  李祥  黎丹  廖承红  杨诺  曾纪峰  郑继平  张立领  余集楠 《热带生物学报》2013,4(4):332-334

亚洲岩蟒主要栖息在热带与亚热带森林,具有较高的药物、营养和经济价值,人工养殖中常因肺炎而死亡.为调查该病病因,对海南省养殖场发病蟒蛇进行心脏穿刺采集血样、细菌培养、革兰氏染色镜检、16S rRNA基因序列测定和BLAST分析等方法寻找病原,结果表明,分离菌株为肺炎克雷伯氏菌.  相似文献   

黄喉拟水龟肺炎克雷伯菌外膜蛋白 A 的原核表达与纯化     

莫超迪  覃呈欢  戴龙喜  韩鹏夫  纪月鑫  韦友传 《广东农业科学》2023,50(3):137-143

【目的】克隆黄喉拟水龟肺炎克雷伯菌外膜蛋白A(KpOmpA)基因,构建重组质粒并诱导其表达,纯化获取重组蛋白KpOmpA,为黄喉拟水龟抗肺炎克雷伯菌的相关疫苗研究奠定基础。【方法】以黄喉拟水龟肺炎克雷伯菌DNA为模板,克隆获得KpOmpA基因后与pET-32a表达载体连接;将构建的重组质粒pET-32aKpOmpA转化大肠杆菌BL21(DE3)感受态细胞,经异丙基-β-D-硫代半乳糖苷（IPTG）诱导,通过SDSPAGE电泳检测KpOmpA重组蛋白的表达情况,并对重组蛋白进行可溶性分析;经Ni-IDA琼脂糖纯化树脂柱纯化KpOmpA重组蛋白,应用Western blot方法鉴定纯化蛋白。【结果】KpOmpA基因CDS序列全长为1 071 bp,推定编码356个氨基酸,与其他肺炎克雷伯菌的OmpA氨基酸序列同源性超过99%,高度保守。重组质粒pET-32a-KpOmpA经双酶切、测序确认基因序列无移码或突变,表明重组质粒成功构建。转化后的BL21感受态细胞经终浓度为1 mmol/L IPTG在37℃下诱导4 h后进行SDS-PAGE检测,结果表明,KpOmpA重组蛋白大量表达,以包涵体形...  相似文献