Detection and genetic diversity of porcine rotavirus A,B and C in eastern Australian piggeries     

B Genz  J Gerszon  Y Pollock  B Gleeson  R Shankar  MJ Sellars  RJ Moser 《Australian veterinary journal》2023,101(4):153-163

Rotaviruses (RV) have a high prevalence in piggeries worldwide and are one of the major pathogens causing severe diarrhoea in young pigs. RV species A, B, and C have been linked to piglet diarrhoea in Australian pig herds, but their genetic diversity has not been studied in detail. Based on sequencing of the structural viral protein 7 (VP7) RVA G genotypes G3, G4 and G5, and RVC types G1, G3, G5, and G6 have been identified in Australian piggeries in previous studies. Although occurrence of RVB was reported in Australia in 1988, no further genetic analysis has been conducted. To improve health management decisions in Australian pig herds, more information on RV prevalence and genetic diversity is needed. Here, 243 enteric samples collected from 20 pig farms within Eastern Australia were analysed for the presence of RV in different age groups using a novel PCR-based multiplex assay (Pork MultiPath™ enteric panel). RVA, RVB, and RVC were detected in 10, 14, and 14 farms, respectively. Further sequencing of VP7 in selected RV-positive samples revealed G genotypes G2, G5, G9 (RVA), G6, G8, G14, G16, G20 (RVB), and G1, G3, G5, G6 (RVC) present. RVA was only detected in young (<10 weeks old) pigs whereas RVB and RVC were also detected in older animals (>11 weeks old). Interestingly, RVB and RVC G-type occurrence differed between age groups. In conclusion, this study provides new insights on the prevalence and diversity of different RV species in pig herds of Eastern Australia whilst demonstrating the ability of the Pork MultiPath™ technology to accurately differentiate between these RV species.  相似文献   

乳腺中脂肪细胞的转化和更迭     

《中国畜牧杂志》2019,(9)

雌性动物的乳腺组织是由胚胎时期的外胚层发育而来,出生时只有少量导管和皮下基质结构,而后在性成熟、妊娠和泌乳期乳腺发育达到峰值,这一特点使乳腺成为出生后唯一可以重复再生的器官。在乳腺发育到退化的循环中,乳腺的上皮细胞、基质白色脂肪细胞、棕色脂肪细胞和肌上皮细胞经历了一系列转化和更迭,脂肪细胞的动态转化反映了乳腺的功能变化。研究乳腺细胞的转化和更迭与母畜的泌乳直接相关,对延续母畜的生产效率有重要意义。本文就乳腺中脂肪细胞转化方面的最新研究进展进行综述,为深度揭示乳腺发育过程中细胞更迭的机制提供前沿研究信息。  相似文献   

Cloning and Sequence Analysis of PTTG1 Gene in Luchuan Pig     

QIN Zhao-xian  NONG Su-qun  GUAN Zhi-hui  LIANG Dan  QIN Qian  LAN Xi  PAN Tian-biao  XIE Bing-kun 《中国畜牧兽医》2016,43(9):2223-2229

This experiment was aimed to clone PTTG1 gene CDS sequence of Luchuan pig,and was analyzed by bioinformatics methods.A pair of special primers was designed according to predicted sequence of porcine PTTG1 in GenBank.The coding sequence of PTTG1 in Luchuan pig was amplified by RT-PCR,its gene sequence characteristics and protein structure was systemically analyzed by bioinformatics techniques.The results showed that the cloned PTTG1 fragment included a 609 bp CDS (coding 202 amino acids).The sequence multi-aligned results showed that Luchuan pig shared 90.15%,87.85%,87.52%,87.03%,76.03%,74.38%,55.74% and 44.48% of similar nucleotide sequence with that of Bos,Pan troglodytes,Homos,Macaca,Rattus,Mus,Gallus and Danio retio,respectively.The protein structure analysis results showed that the protein attributed hydrophilic protein without signal peptide,localized in cell cytoplasm and had 16 phosphorylation sites.The phylogentic tree of amino acid indicated that PTTG1 was highly conserved in the process of evolution of different species.The cloning and analysis of PTTG1 gene provided an important foundation for further study biological function of porcine PTTG1 during early embryonic development.  相似文献   

Study on Developmental Expression of ApoCⅡ Gene mRNA in Liver Tissues of Pigs     

LIU Cui-cui  GUO Xiao-hong  CAO Guo-qing  GAO Peng-fei  PU Zhong-de  NIU Jiao-yan  ZHANG Qi  LI Meng  JIN Yu-shu  LI Bu-gao 《中国畜牧兽医》2015,42(10):2726-2731

The aim of this study was to investigate the developmental patterns of ApoCⅡ gene mRNA in liver in Mashen and Large White pigs, and study the relationship between the expression level of ApoCⅡ and the lipid metabolism in pigs.The mRNA relative expressions of ApoCⅡ gene in liver at seven stages of 1,30,60,90,120,150,and 180-day old in Mashen and Large White pigs were determined by quantitative Real-time PCR.The results showed that the developmental trend of ApoCⅡ mRNA expression in liver between Mashen and Large White pigs was different.The ApoCⅡ mRNA abundance was decreased from birth to 60-day old,then increased at 90-day old,and decreased again after that in Mashen pig.However,the relative expression amount in Large White pig was gradually decreased from birth to 150-day old and increased again at 180-day old.Except for the ApoCⅡ mRNA expression amount at 1-day old,the differences of the expression amount at other stages in Mashen and Large White pigs were significant or extremely significant (P<0.05 or P<0.01).The ApoCⅡ mRNA expression in liver was affected by age and breed,and could play an important role in lipid metabolism in pigs.  相似文献   

基于股份减持中的大股东隧道行为实证研究     

傅利平  张大勇 《湖南农业大学学报(自然科学版)》2011,(5):39-43

以2007~2009年被大股东减持的沪深A股上市公司作为样本，运用OLS与Logistic回归方法，实证分析了大股东通过操纵重大信息披露在股份减持过程中的隧道行为。得出了在减持前30个交易日被大股东减持的上市公司有显著的正累计超常收益，但减持后30个交易日则出现显著的负累计超常收益；被大股东减持的上市公司在减持前披露“利好”消息、减持后披露“利空”消息的概率高；民营控股上市公司大股东操纵上市公司信息披露的概率较高；公司估值(托宾值)越高，大股东信息操纵行为的概率也越大；被减持公司的净资产收益率(ROE)与总资产收益率(ROA)对大股东信息操纵行为的概率不存在显著影响等结论。  相似文献   

南方旱地作物栽培模式及发展趋势     

杨克相  罗阳春  方芳  蒲春蕾  罗峰  曾先堂  吴迅  何川  李钟  明充 《安徽农学通报》2016,22(6)

该文介绍了20世纪80年代以来,我国南方旱地主要作物栽培模式的演变形式,不同栽培模式的技术要点。根据现代农业发展的迫切需求、中国农村劳动力严重缺乏等现状,解析了当前农村土地流转的实际形式以及取得的部分成绩,展望了南方旱地农业,农村产业发展的方向。为南方旱地农业的发展提供借鉴。  相似文献   

重组猪抑制素蛋白质中毒素残留检测及其理化稳定性     

李辉  翟颖超  窦晓  张甜  果双双  施振旦 《江苏农业学报》2015,31(2)

为探索纯化的重组猪抑制素蛋白质毒素残留情况以及重组猪抑制素蛋白质稳定性,本研究利用鲎试剂、气相色谱和SDS-PAGE方法对重组猪抑制素蛋白质中的细菌内毒素、β-巯基乙醇的残留量及其蛋白质在不同温度、极端p H值和反复冻融条件下的稳定性进行检测。结果显示,经过3次等电点沉淀洗涤后,重组猪抑制素蛋白质中细菌内毒素和β-巯基乙醇的残留量大幅减少。通过在不同温度下的处理发现,重组猪抑制素蛋白质在4℃环境下较稳定,可以长期保存;但在37℃时,稳定性快速下降,随着温度上升而降解加速。通过极端p H值和反复冻融试验发现,重组猪抑制素蛋白质对极端p H值和反复冻融具有较好的耐受性。  相似文献   

新生仔猪的护理——以上海某规模猪场为例     

石保玉  张振玲 《猪业科学》2020,37(10):114-116

我国养猪业发展迅速，整体水平在不断提高，但是与养殖业较为发达的国家相比仍有一些差距。以每年每头母猪断奶的仔猪数（PSY）为例，目前国外养猪发达国家PSY普遍已超30头，而我国却仍在25头左右，仍有较大的提升空间。仔猪护理工作在生猪生产过程中尤为重要，一旦护理措施不当就会造成仔猪死亡，从而降低PSY，直接影响到养猪企业的经济效益。规模猪场仔猪死亡的主要原因就是由于仔猪护理不当所引起的，仔猪护理问题一直是养猪业的一个难题。文内以2020年4—5月上海某规模猪场某栋产房新生仔猪（共477头，其中压死8头，瘦弱致死12头，腹泻致死亡的32头）为例，介绍规模猪场的仔猪护理及注意事项，以期为仔猪护理工作提供借鉴。  相似文献   

猪胆囊中沙门菌L型的分离培养与invA基因检测     

佘晓玲  王涛  丁文静  吴文娟  潘耀振  汤可立  王丹霓  王和 《动物医学进展》2015,(3):25-28

为了解猪胆囊中沙门菌L型携带情况,在贵阳市屠宰场采集970例健康生猪的胆囊组织与胆汁标本,用常规细菌学方法和非高渗分离培养法分离沙门菌及其细菌L型,用PCR和核酸序列分析方法对稳定L型纯培养物进行沙门菌的invA基因检测。结果显示,970例生猪胆囊标本未检出沙门菌细菌型,细菌L型检出率为8.25%;80例细菌L型分离物中有50例invA检测阳性,占5.15%;占细菌L型阳性分离物62.50%。研究结果为生猪胆囊沙门菌L型感染的流行病学及其检查提供了依据。  相似文献   

莱芜猪胴体切块质量预判     

张伟力 《养猪》2014,(2):63-64

本文以莱芜猪肉质育种为例，介绍了在屠宰线上对胴体的肉色、大理石纹、特别是肌内脂肪进行主观评定预测的简单易行的方法，其结果可用于肉质选种选育。  相似文献