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41.
Camila Lehnhardt Pires Selma Dzimidas Rodrigues Daniel Bristot Henrique Hessel Gaeta Daniela de Oliveira Toyama Wladimir Ronald Lobo Farias Marcos Hikari Toyama 《Marine drugs》2013,11(3):934-943
The sulfated polysaccharides from Solieria filiformis (Sf), Botryocladia occidentalis (Bo), Caulerpa racemosa (Cr) and Gracilaria caudata (Gc) were extracted and extensively purified. These compounds were then subjected to in vitro assays to evaluate the inhibition of these polysaccharides on the growth of Leishmania (L.) amazonensis promastigotes. Under the same assay conditions, only three of the four sulfated polysaccharides were active against L. amazonensis, and the polysaccharide purified from Cr was the most potent (EC50 value: 34.5 μg/mL). The polysaccharides derived from Bo and Sf demonstrated moderate anti-leishmanial activity (EC50 values of 63.7 μg/mL and 137.4 μg/mL). In addition, we also performed in vitro cytotoxic assays toward peritoneal macrophages and J774 macrophages. For the in vitro cytotoxicity assay employing J774 cells, all of the sulfated polysaccharides decreased cell survival, with CC50 values of 27.3 μg/mL, 49.3 μg/mL, 73.2 μg/mL, and 99.8 μg/mL for Bo, Cr, Gc, and Sf, respectively. However, none of the sulfated polysaccharides reduced the cell growth rate of the peritoneal macrophages. These results suggest that macroalgae contain compounds with various chemical properties that can control specific pathogens. According to our results, the assayed sulfated polysaccharides were able to modulate the growth rate and cell survival of Leishmania (L.) amazonensis promastigotes in in vitro assays, and these effects involved the interaction of the sulfated polysaccharides on the cell membrane of the parasites. 相似文献
42.
利什曼原虫实时荧光定量PCR方法的建立 总被引：1，自引：0，他引：1
以利什曼原虫的小环状动基体DNA(Leishmaniak DNA)为靶基因,建立实时荧光定量PCR检测利什曼原虫的方法。根据GenBank报道的利什曼原虫小环状动基体DNA保守序列设计合成特异性引物,经PCR扩增后与pMD18-T载体连接,转化入大肠杆菌DH5α感受态细胞。挑选阳性克隆重组质粒经鉴定正确后,作为模板建立SYBR-GreenⅠ荧光定量PCR标准曲线和熔解曲线。结果构建的标准曲线线性关系良好,相关系数为0.996,而且特异性强,重复性好。本研究成功建立了实时荧光定量PCR检测利什曼原虫的方法,该方法可用于利氏曼原虫病的诊断、流行病学监测和科学研究。 相似文献
43.
原虫是一种单细胞真核生物,整个虫体由一个细胞构成,可以寄生在机体的腔道、体液、组织或细胞内。原虫病严重危害人类健康和养殖业发展。目前,广泛使用的化学药物治疗会产生耐药性、药物残留和毒副作用等弊端。青蒿素作为一种从植物黄花蒿中提取的天然物质,对多种原虫具有治疗作用,与化学药物相比更加安全、廉价甚至更高效。本文对青蒿素在原虫病治疗中的作用和机理进行综述,旨在为其应用于原虫病治疗提供理论依据。 相似文献
44.
The clinical signs and histopathological features of a primary extragenital canine transmissible venereal tumour (TVT) are described. Three subcutaneous round alopecic nodules were located on the anterior and caudal dorsal region and in the ventral area of the neck. Cytologically, tumour cells were intermediate in size with a moderate amount of cytoplasm, and the nuclei were immature with finely reticular chromatin. The cytoplasm was lightly to heavily basophilic and contained distinct small vacuoles at the periphery. On the basis of these characteristics, a diagnosis of TVT was made and confirmed by histological and ultrastructural investigations. Leishmania amastigotes were detected in the cytoplasm of macrophages and neoplastic cells of the tumoral mass. The presence of the parasite within neoplastic cells is consistent with a histiocytic origin of TVT. 相似文献
45.
Proliferative glomerulonephritis in experimental Leishmania donovani infection of the golden hamster
W.E Agu J.P Farrell E.J.L Soulsby 《Comparative immunology, microbiology and infectious diseases》1981,4(3-4)
Extensive kidney involvement is reported in golden hamsters infected with Leishmania donovani. The lesion is characterised grossly by pale and enlarged kidneys, and histologically by thickening of the basal lamina of the glomerulus, distended convuluted tubules containing protein casts, constricted glomerular capillaries and infiltration of the interstitial tissues with mononuclear cells. Quantitative measurement of the glomerular cells labelled in vivo with tritiated thymidine showed a striking increase in the glomerular cell proliferation as the infection progressed. Using direct immunofluorescent technique, granular deposits of IgG, complement C3 and fibrinogen were detected in the glomeruli of the infected animals. A positive protamine sulphate test, as well as histological evidence, showed that disseminated intravascular coagulation had occurred in the infection. Although the antigens have not been identified, antigen-antibody complexes are strongly implicated in the pathogenesis of the proliferative glomerulonephritis observed in this study. 相似文献
46.
Ikeda-Garcia FA Lopes RS Ciarlini PC Marques FJ Lima VM Perri SH Feitosa MM 《Research in veterinary science》2007,83(1):105-108
The present study aimed to evaluate the renal and hepatic responses in eight dogs with visceral leishmaniasis submitted to treatment with meglumine antimoniate and to verify the occurrence of possible side effects. Urinalysis, hepatic and renal function tests were carried out in all animals at up to seven moments. After the end of a six-month observation period, all dogs were euthanized. Before the beginning of the experiment urinary and biochemical alterations were observed in four dogs due to the changes caused by the parasite itself. These alterations included the presence of renal cells, cylindruria, proteinuria, azotemia, hyperproteinemia and hypoalbuminemia. One dog died on the third day after treatment because an aggravation of the clinical picture, probably due to the medication. During the course of the study, an increase in hepatic enzymes was verified in two animals. Sixty days after the beginning of the treatment four dogs showed remission of clinical signs. The other three were asymptomatic with persistent biochemical alterations. From these, two presented recurrence of clinical signs about 150 days after the beginning of the treatment while in the other, hyperproteinemia persisted. Meglumine antimoniate was not efficient to treat dogs with severe renal dysfunction and the side effects observed were pain at the site of injection and the probable transient hepatotoxicity, evidenced by biochemical examinations, but without the presence of clinical signs. 相似文献
47.
Real-time PCR assay in Leishmania-infected dogs treated with meglumine antimoniate and allopurinol 总被引：1，自引：0，他引：1
Manna L Reale S Vitale F Picillo E Pavone LM Gravino AE 《Veterinary journal (London, England : 1997)》2008,177(2):279-282
A real-time PCR assay was exploited for monitoring the Leishmania DNA load in different tissues from 18 naturally-infected dogs before and after treatment with a combination of meglumine antimoniate (100mg/kg/day, subcutaneously) and allopurinol (10mg/kg/day, orally) for 30 days. After the combined therapy, allopurinol was continued at the same dose until the end of the observation period. Whole blood samples, lymph node aspirates, and skin biopsies were collected at the time of diagnosis, 1 month after starting therapy, and every 3 months for 2 years. In six dogs parasite load assessments continued every 6 months for a further 3 years. At each assessment, the dogs were examined for signs of disease and a clinical score was recorded. At diagnosis, the highest Leishmania DNA load was detected in lymph node aspirates. From 1-6 months post-therapy a general improvement in clinical conditions was recorded in all dogs, which correlated with a decrease in the parasite DNA load in all tested tissues, even though it was less pronounced in lymph node aspirates. In the period from 9-24 months post-therapy, a re-increase in parasite load was observed in the tissues of some dogs, concomitant with a disease relapse. The results show that the combined therapy with meglumine antimoniate and allopurinol promoted a clinical improvement which was accompanied by a reduction in the parasitic load in the blood, skin and lymph nodes but, even after long period of allopurinol administration alone, Leishmania may persist in dog tissues. 相似文献
48.
A.A. da Silva Filho D.O. Resende M.J. Fukui F.F. Santos P.M. Pauletti W.R. Cunha M.L.A. Silva L.E. Gregrio J.K. Bastos N.P.D. Nanayakkara 《Fitoterapia》2009,80(8):478-482
Baccharis dracunculifolia (Asteraceae), the most important plant source of the Brazilian green propolis (GPE), displayed in vitro activity against Leishmania donovani, with an IC50 value of 45 µg/mL, while GPE presented an IC50 value of 49 µg/mL. Among the isolated compounds of B. dracunculifolia, ursolic acid, and hautriwaic acid lactone showed IC50 values of 3.7 µg/mL and 7.0 µg/mL, respectively. Uvaol, acacetin, and ermanin displayed moderate antileishmanial activity. Regarding the antiplasmodial assay against Plasmodium falciparum, BdE and GPE gave similar IC50 values (about 20 µg/mL), while Hautriwaic acid lactone led to an IC50 value of 0.8 µg/mL (D6 clone). 相似文献
49.
Crude extracts and column fractions from the red algae Asparagopsis taxiformis and A. armata from the Strait of Messina (Italy) were screened for the production of antimicrobial compounds. Extracts from both species revealed remarkable antiprotozoal activity against Leishmania, revealing such algae as a great source of natural antiprotozoal products. 相似文献
50.