全血法鸡淋巴细胞转化试验最佳试验条件的研究   总被引：17，自引：0，他引：17  

吴建设  郑世军 《畜牧兽医学报》1997,28(3):212-216

通过丝裂原浓度、血液稀释度、灭活犊牛血清浓度和培养时间四个参数的测定，确定了鸡全血淋巴细胞转化试验的最佳培养条件。最佳试验方法如下：采用不含灭活犊牛血清的ＲＰＭＩ１６４０培养液，刺激管中ＣｏｎＡ浓度为４５μｇ／ｍｌ或ＬＰＳ浓度为２５μｇ／ｍｌ，血液稀释度为１：２０，４０℃培养５６ｈ，在培养结束前１６ｈ加＾３Ｈ－胸腺嘧啶核苷（＾３Ｈ－ＴｄＲ）。本法简单方便，可检测Ｔ、Ｂ细胞的功能状况及用于鸡病病理发  相似文献   

Cycloartane saponins from Astragalus peregrinus as modulators of lymphocyte proliferation     

Luisella Verotta  Marco Guerrini  N. A. El-Sebakhy  Aya M. Asaad  Soad M. Toaima  Moahamed E. Abou-Sheer  Ying-De Luo  John M. Pezzuto 《Fitoterapia》2001,72(8)

From Astragalus peregrinus, four cycloartane-type saponins have been isolated and their structures elucidated by spectral means as 20(R),24(S)-epoxy-9β,19-cyclolanostane-3β,6α,16β,25-tetrol 3-O-β- -glucopyranoside (1), 20(R),24(S)-epoxy-9β,19-cyclolanostane-3β,6α,16β,25-tetrol 3-O-α- -rhamnopyranosyl-(1→4)-β- -glucopyranoside (2), 20(R),24(S)-epoxy-9β,19-cyclolanostane-3β,6α,16β,25-tetrol 3-O-α- -rhamnopyranosyl-(1→2)-β- -glucopyranoside (3) and 20(R),25-epoxy-9β,19-cyclolanostane-3β,6α,16β,24(S)-tetrol (24-O-acetyl)- 3-O-α- -rhamnopyranosyl-(1→2)-(6′-O-acetyl)-β- -glucopyranoside (4). Compounds 2 and 3 showed to stimulate the proliferation of mouse splenocytes and were not significantly cytotoxic.  相似文献   

Lymphocyte transformation test in veterinary clinical immunology     

Ota Barta  Priscilla P Oyekan 《Comparative immunology, microbiology and infectious diseases》1981,4(2):209-221

Lymphocyte transformation test is a powerful tool in laboratory testing of immunologic competence of animals. The impaired function of the lymphocytes or presence of mitogenesis suppressing factors in the patient serum were detected by comparing lymphocyte transformation (expressed as thymidine incorporation) obtained in media containing either autologous, homologous, or fetal calf serum additions. Most valuable results were obtained by using at least two, preferably three, different phytomitogens: concanavlin A (Con A), pokeweed mitogen (PWM), and pl ytohemagglutinin (PHA) at optimal concentrations (Con A, 15 μg/ml, PWM and PHA, 5 μg/ml) and decreased concentrations (Con A, 5 μg/ml, PWM and PHA, 1 μg/ml). Mitogenesis induced by lipopolysaccharide was considerably smaller and not used routinely. With 2 × 10⁵ lymphocytes/well, the background count of unstimulated lymphocytes in autologous serum in healthy dogs was usually between 100 and 400 counts/min (CPM), in clinically healthy cattle and horses from 200 to over 2000 CPM. Higher CPM were rarely detected without clinical disease. Increased background counts were often associated with viral infections, leukemias and lymphoreticular hyperplasias, decreased background counts were associated with various diseases. The stimulation indexes (SI) of healthy animals in autologous serum with Con A, (5 μg/ml) or PWM or PHA (1 μg/ml) were in the range from 100 to 1000 in the dogs, in the tens for Con A and in hundreds for PWM and PHA in horses and cattle. Increased SI were present during the incubation period of various diseases. Decreased SI were associated with numerous infectious and lymphoreticular diseases and were caused by any of the following: (1) the presence of serum immunosuppressive factor(s) in the patient serum, (2) the decreased response of lymphocytes to mitogens, or (3) increased mitogenicity of lymphocytes due to unidentified serum factors in absence of phytomitogens.  相似文献   

江西麦饭石在免疫学上的研究(Ⅰ)——对兔细胞免疫的影响     

胡介卿  张文光 《江西农业大学学报》1992,(2)

本试验证实,江西麦饭石对兔淋巴细胞转化功能有明显的作用,可以推测,江西麦饭石中含有某些非特异性免疫促进剂。  相似文献   

In vitro stimulation of bovine milk lymphocytes standardization of the assay for bovine brucellosis     

L.F Ayanwale  J.B Kaneene  D.W Johnson  C.C Muscoplat  R.K Anderson 《Comparative immunology, microbiology and infectious diseases》1981,4(3-4):343-352

Lymphocytes of bovine milk origin were investigated by immunostimulation in vitro to standardize the assay for measuring the immune responses of the cells which might be useful in further understanding the immunopathology and diagnosis of bovine brucellosis. The lymphocytes were separated from whole freshly collected milk by centrifugation. The pellet of lymphocytes was washed in RPMI-1640 medium, cultured at different concentrations for different days and with Brucella abortus soluble antigen strain 1119-3 and Concanavalin A. Each culture was labelled with 1.0 μCi of methyl-[³H]thymidine 16–18 hours prior to termination of incubation at 37 C. Termination was done by cooling to 4 C. The cells were harvested for liquid scintillation counting spectrometry. In the groups of calfhood vaccinated cows and nonexposed milkers, a milk lymphocyte concentration of 2.0 × 10⁶/ml of medium yielded a statistically significant blastogenesis. The Brucella abortus soluble antigen concentration of 4.4 μg of protein/well was found optimal to induce significant immunostimulation. A period of 4 days of incubation of the milk lymphocyte in the test was found optimal in inducing statistically significant blastogenesis in this system.  相似文献   

仔猪痢清对动物细胞免疫功能影响的初步研究     

洪伟鸣  邢晓玲  郁杰  葛竹兴  王妲妲 《安徽农业科学》2009,37(1):142-143

[目的]研究仔猪痢清口服液对动物细胞免疫功能的影响。[方法]分别采用MTT法和中性红吞噬法测定仔猪痢清口服液对猪淋巴细胞转化和小鼠腹腔巨噬细胞吞噬作用的影响。[结果]给药组猪淋巴细胞的转化显著高于对照组;给药组小鼠腹腔巨噬细胞活性与生理盐水对照组相比有显著性差异。[结论]该口服液具有良好的促进ConA诱导的猪外周血T淋巴细胞转化作用和促进小鼠腹腔巨噬细胞吞噬中性红作用,表明其具有良好的免疫增强效果。  相似文献   

Changes in Regulatory T Cells in Dogs with Cancer and Associations with Tumor Type     

K. O'Neill  A. Guth  B. Biller  R. Elmslie  S. Dow 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2009,23(4):875-881

Background: Regulatory T cells (Treg) have been shown to suppress antitumor immunity and often are increased in humans and rodents with cancer. However, Tregs have not been well studied in dogs with cancer and it is not known if certain tumor types are associated with increased Tregs.
Hypothesis: We hypothesized that Treg percentages would be increased in dogs with cancer and that Treg percentages would be higher in dogs with certain types of cancer.
Animals: The percentages and numbers of Tregs and nonregulatory T cells and B cells were assessed in 34 dogs with cancer and 9 age-matched control dogs. Dogs evaluated included 14 dogs with sarcoma, 7 dogs with carcinoma, 7 dogs with lymphoma, and 6 dogs with mast cell tumor.
Methods: Numbers and percentages of Tregs, CD4⁺, and CD8⁺ T cells and B cells were determined using flow cytometry and compared between control dogs and dogs with cancer.
Results: The percentage of Tregs was significantly increased overall in dogs with cancer compared with control dogs. When tumor types were compared, Treg percentages were significantly increased in dogs with carcinoma. The Treg/CD8 T cell ratio was significantly higher in dogs with cancer compared with control dogs and was also significantly increased in 2 dogs with T-cell lymphoma.
Conclusions: Treg percentages in blood were increased in dogs with cancer, particularly in dogs with carcinoma. The Treg/CD8 ratio also identified tumor-specific abnormalities in dogs with cancer. These findings indicate that tumor-specific factors may affect Tregs in dogs.  相似文献   

What is your diagnosis? Fine‐needle aspirate of a third eyelid mass in a Paint horse     

Gupta A  Bhaskaran M  Storey E  Carter RT  Wakamatsu N  Gaunt SD 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2012,41(2):299-300

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A comparison of the lymphocyte subpopulations of pigs experimentally infected with porcine circovirus 2 and/or parvovirus   总被引：3，自引：0，他引：3  

Kim J  Chae C 《Veterinary journal (London, England : 1997)》2003,165(3):325-329

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The role of flow cytometry in companion animal diagnostic medicine     

Tarrant JM 《Veterinary journal (London, England : 1997)》2005,170(3):278-288

Flow cytometry is a powerful tool for characterising the composition of complex cell populations. The accuracy and precision of this technology for describing and enumerating cells exceeds traditional methods. The number of diagnostic veterinary laboratories with access to a dedicated machine is increasing, and there is the potential to offer a clinical flow cytometry service. The improved availability of monoclonal antibodies (mAb) to cell markers expressed by the leukocytes of companion animals, permits the implementation of comprehensive mAb panels suitable for diagnosis of lympho- and myeloproliferative disease. Reticulated erythrocyte and platelet quantification, antiglobulin assays for immune-mediated cytopenias, lymphocyte subset analysis, and immunophenotyping of lymphoma and leukemia, have been validated for companion animal samples on the flow cytometer. It is now timely to consider the role of flow cytometry in diagnostic practice, and the requirement for quality assurance and standardization of testing procedures.  相似文献