农林高校开展专业建设的研究与实践     

翟雪峰  李德才  姜中珠  孙新竹 《安徽农业科学》2014,(9):2818-2819

以东北林业大学为例,阐述了专业建设的几点看法.认为要加强专业建设,必须依托国家级重点学科、建设高水平师资队伍、优化人才培养方案、创新人才培养模式、对重点专业进行建设等措施,不断提高学校专业的整体建设水平.  相似文献   

溃疡病菌诱导下毛白杨防御相关基因的应答反应     

张秀英  宋瑞清  理永霞  吕全  刘振宇  张星耀 《安徽农业科学》2014,(12):3555-3558,3575

[目的]研究溃疡病菌诱导下毛白杨防御相关基因的应答反应.[方法]以毛白杨为试验材料,用溃疡病菌对7月龄幼苗进行接种,提取诱导前后的毛白杨树皮mRNA,通过反转录生成cDNA,构建72 h差异表达的SSH-cDNA文库.随机挑选199个阳性克隆进行测序,将测序结果与NCBI基因库中序列进行比较,并对这些EST在dbEST数据库进行同源检索分析,利用RT-qPCR对文库中7个基因进行进一步分析.[结果] 172个EST找到了同源序列,其中有32个序列与防御相关.在毛白杨受到溃疡病菌诱导时,这些防御基因的表达量明显上升.[结论]该研究为开展毛白杨抗溃疡病重要功能基因的克隆和鉴定奠定了基础.  相似文献   

稻瘟病抗病基因定位及克隆研究进展     

赵家铭  张丽霞  郑文静 《辽宁农业科学》2014,(2):47-49

稻瘟病是水稻生产中最严重的真菌病害,严重影响着水稻的产量。随着DNA分子标记的不断发展及水稻基因组测序的完成,稻瘟病抗病基因的定位克隆取得了突破性的进展,为稻瘟病抗病机理研究以及稻瘟病抗性育种奠定了重要基础。本文综述了稻瘟病抗病基因定位及克隆的研究进展,对稻瘟病抗病基因的研究成果在实践中的应用提出了展望。  相似文献   

植物耐盐基因研究及其在棉花上的应用     

张安红  王志安  肖娟丽  罗晓丽 《山西农业科学》2014,(6):640-642,652

盐害会对植物生长造成严重的危害、对农作物的产品品质和产量造成很大的损失。通过调控耐盐相关基因的表达,能提高转基因植物的耐盐能力。近年来,人们克隆了许多与耐盐相关的基因,主要包括渗透调节物合成基因、耐盐相关蛋白类基因、保护酶相关基因、转录因子的调控基因。主要综述了近年来植物耐盐相关基因的克隆及其在棉花基因工程中的研究应用等方面的一些进展,并对棉花耐盐基因工程的研究应用提出了对策和展望。  相似文献   

蔷薇科S-RNase基因型鉴定引物在仁用杏基因组中的通用性分析          下载免费PDF全文

刘梦培  乌云塔娜  朱高浦  赵罕  杜红岩 《林业科学研究》2014,27(6):860-865

正仁用杏为我国六大木本粮油战略性树种之一,是"三北"地区适应性最强、发展潜力最大的重要生态经济树种之一,被誉为"铁杆庄稼",其抗逆性强,也是集抗旱、抗寒(可低至-35℃)、抗风沙为一体的"先锋"树种。杏仁油中不饱和脂肪酸含量95%左右,其中单不饱和脂肪酸——油酸含量在70%以上,与橄榄油相近,富含丰富的蛋白质、不饱和脂肪  相似文献   

高等植物的花发育模型及其基因调控研究进展     

田云芳  袁秀云  蒋素华  马杰  崔波 《贵州农业科学》2014,(11)

花发育是高等植物生长发育过程中非常重要的事件之一。为了解花发育的分之机制,阐述了花器官决定同源异型基因作用模型的产生和发展过程,如经典的花发育 ABC 模型、随后发展起来的 ABCD、ABCDE 和四聚体模型,综述了花器官发育分子模型同源基因的调控机理等方面的研究成果,并展望了研究方向。  相似文献   

早酥梨病程相关基因非表达子1基因（NPR1）克隆及原核表达     

张范  张朝红  徐炎  王跃进 《农业生物技术学报》2010,18(1):18-23

本研究以成年早酥梨(Pyrus bretschneideri cv.Zaosu)叶片为材料,通过RT-PCR克隆早酥梨病程相关基因非表达子1基因(NPR1)并获得其全长序列1771 bp,开放阅读框为1761 bp,编码586个氨基酸(GenBank登录号:FJ769372).利用NCBI/Blastp和ClustalX软件进行相似性分析表明,目的基因编码蛋白质序列与日本梨(p.pyrifolia)、秋子梨(P.ussuriensis)、苹果(Malus xdomestica)、烟草(Nicotiana tabacum)和拟南芥(Arabidopsis thaliana)的NPR1蛋白相似性分别为99%、98%、98%、67%和59%.将其连接pGEX-4T-1原核表达载体并转化大肠杆菌(EScherichia coli)BL21,经IPTG诱导表达获得大小约为91 kD的目的融合蛋白,融合蛋白主要以包涵体形式表达,表达量约占总蛋白17%.  相似文献   

Does reduced MHC diversity decrease viability of vertebrate populations?     

Jacek Radwan  Aleksandra Biedrzycka 《Biological conservation》2010,143(3):537-544

Loss of genetic variation may render populations more vulnerable to pathogens due to inbreeding depression and depletion of variation in genes responsible for immunity against parasites. Here we review the evidence for the significance of variation in genes of the Major Histocompatibility Complex (MHC) for conservation efforts. MHC molecules present pathogen-derived antigens to the effector cells of the immune system and thus trigger the adaptive immune response. Some MHC genes are the most variable functional genes in the vertebrate genome. Their variation is clearly of adaptive significance and there is considerable evidence that its maintenance is mainly due to balancing selection imposed by pathogens. However, while the evidence for selection shaping MHC variation on the historical timescale is compelling, a correlation between levels of MHC variation and variation at neutral loci is often observed, indicating that on a shorter timescale drift also substantially affects MHC, leading to depletion of MHC diversity. The evidence that the loss of MHC variation negatively affects population survival is so far equivocal and difficult to separate from effects of general inbreeding. Some species with depleted MHC variation seem to be particularly susceptible to infection, but other species thrive and expand following severe bottlenecks that have drastically limited their MHC variation. However, while the latter demonstrate that MHC variation is not always critical for population survival, these species may in fact represent rare examples of survival despite of the loss of MHC variation. There is clearly a compelling need for data that would disclose the possible consequences of MHC diversity for population viability. In particular, we need more data on the impact of MHC allelic richness on the abundance of parasites or prevalence of disease in populations, while controlling for the role of general inbreeding. Before such evidence accumulates, captive breeding programs and other conservation measures aimed at inbreeding avoidance should be favoured over those protecting only MHC variation, especially since inbreeding avoidance programs would usually conserve both types of genetic diversity simultaneously.  相似文献   

地籍管理专业课程教学模式探讨     

路婕  冯新伟  吕巧灵 《安徽农学通报》2011,17(7):201-202,212

采取传统的专业课讲授模式会降低学生解决实际问题的能力,因此,需要改革传统的专业课教学模式,以适应当今社会对大学毕业生的要求。该文从＂地籍管理＂这门专业课的教学实践出发,指出专业课程教学定位要注重培养学生学习技能,并从培养学习兴趣、引导学生学会思考、设计综合实验、应用信息技术、培养学生热情、积极的生活态度等几个方面探索了土地资源管理专业课程教学模式的构建。  相似文献   

Pseudomonas putida BP25 alters root phenotype and triggers salicylic acid signaling as a feedback loop in regulating endophytic colonization in Arabidopsis thaliana     

《Physiological and Molecular Plant Pathology》2016

Endophytic Pseudomonas putida BP25 (PpBP25) triggered density dependent alterations on Arabidopsis thaliana Col-0 growth. Endogenous colonization of PpBP25 was found regulated within Arabidopsis that caused induction and repression of 131 and 74 plant genes, respectively. Induced genes like WRKY33, AtRLP19, ATL2, ATEXO70B2, pEARLI, RPS2, CBP60G, PLA2, CRK18, ATFBS1, DREB2A, TIR, RAP2.4, and MOS1 were components of defense and salicylic acid (SA) signaling. Development associated genes were found significantly repressed. Biased activation of phytohormone signaling with their associated fitness costs on plant growth was observed. The data suggests that PpBP25 colonization triggered expression of defense genes that restricted its own population in a feedback loop besides causing altered root phenotype.  相似文献