香蕉柠檬酸合酶基因MaGCS的克隆及生物信息学分析     

迟光红  周雪丽  李美英  徐碧玉  金志强 《热带作物学报》2009,29(8):12-18

采用RACE-PCR的方法首次在香蕉中获得一个柠檬酸合酶基因的cDNA序列。经序列测定和Blastx比对分析表明，该cDNA全长1 885bp，编码513个氨基酸残基，具有植物柠檬酸合酶基因的特征结构域，并与其他植物来源的乙醛酸循环体的柠檬酸合酶具有很高的序列相似性，将其命名为MaGCS(Musa glyoxysomal citrate synthase)，并对其进行生物信息学分析，初步预测其理化性质及功能等。  相似文献   

Bulked Segregant Analysis to Detect QTL Related to Heat Tolerance in Rice （Oryza sativa L.） Using SSR Markers   总被引：3，自引：0，他引：3  

ZHANG Gui-lian  CHEN Li-yun  XIAO Guo-ying  XIAO Ying-hui  CHEN Xin-bo  ZHANG Shun-tang 《中国农业科学(英文版)》2009,8(4):482-487

The study was undertaken to assess the genetic effect of quantitative trait loci （QTLs） conferring heat tolerance at flowering stage in rice. A population consisting of 279 F2 individuals from the cross between 996, a heat tolerant cultivar and 4628, a heat-sensitive cultivar, was analyzed for their segregation pattern of the difference of seed set rate under optimal temperature condition and high temperature condition. The difference of seed set rate under optimal temperature condition and high temperature condition showed normal distribution, indicating the polygenic control over the trait. To identify main effect of QTL for heat tolerance, the parents were surveyed with 200 primer pairs of simple sequence repeats （SSR）. The parental survey revealed 30% polymorphism between parents. In order to detect the main QTL association with heat tolerance, a strategy of combining the DNA pooling from selected segregants and genotyping was adopted. The association of putative markers identified based on DNA pooling from selected segregants was established by single marker analysis （SMA）. The results of SMA revealed that SSR markers, RM3735 on chromosome 4 and RM3586 on chromosome 3 showed significant association with heat tolerance respectively, accounted for 17 and 3% of the total variation respectively. The heat tolerance during flowering stage in rice was controlled by multiple gene. The SSR markers, RM3735 on chromosome 4 and RM3586 on chromosome 3 showed significant association with heat tolerance respectively, accounted for 17 and 3% of the total variation respectively. The two genetic loci, especially for RM3735 on chromosome 4, can be used in marker-assistant-selected method in heat tolerance breeding in rice.  相似文献   

Does the Sulfur Assimilation Pathway Play a Role in the Response to Fe Deficiency in Maize (Zea mays L.) Plants?     

《Journal of plant nutrition》2013,36(10-11):2111-2121

Abstract

The finding that the methionine is the sole precursor of the mugineic acid family phytosiderophores induced us to evaluate whether sulfur assimilation pathway has a role in plant response to Fe deficiency. Maize plants were grown for 10 days in nutrient solution (NS) containing 80 µM Fe in the presence (+S) or absence (?S) of sulfate. After removing the root extraplasmatic iron pool, half of the plants of each treatment (+S and ?S) were transferred to a new Fe deficient NS (0.1 µM final Fe concentration) (?Fe). The remaining plants of each pre‐culture condition (+S and ?S) were transferred to a new NS containing 80 µM Fe (+Fe). Leaves were collected 4 and 24 hours from the beginning of Fe deprivation period and used for chemical analysis and enzyme assays. Results showed that iron content in the leaves was lower in plants grown in S‐deficiency than in those grown in the presence of the macro‐nutrient. Iron deprivation produced an increase in the level of SH compounds in both nutritive conditions (+S and ?S). These observations are suggestive of some relationship between S nutrition and Fe uptake. For this reason, we next investigated the influence of Fe availability on S metabolism through the evaluation of changes in ATPs and OASs activity, the first and the last enzyme of S assimilation pathway respectively. Results showed that S‐starvation increased the activity of both enzymes, but this effect disappeared in plants upon Fe deficiency suggesting that S metabolism is sensitive to Fe availability. Taken together these evidences suggest that S metabolism is sensitive to soil Fe‐availability for plant nutrition and support the hypothesis of S involvement in plant response to Fe deprivation.  相似文献   

Fruit Yield,Plant Growth and Nutrient Status in Mango     

《International Journal of Fruit Science》2013,13(4):3-21

Abstract

Fruit yield and nutrient concentration of mango (Mangifera indica L.) cultivar ‘Osteen’ were studied in relation to rootstocks Gomera-1 and Gomera-3 for three years. The results indicated that the Gomera-3 root system had higher nutrient uptake and transport towards the scion. Data from grafted and ungrafted Gomera-1 and Gomera-3 trees proved similar with respect to N, P, and K utilization profile, with post-harvest accumulation and a decline during flowering, P and K concentrations significantly decreasing during fruit growth. These trends were largely the opposite for Ca and Mg. The Fe, Zn, Mn, and Cu concentrations rose during dormancy and fell at flowering. Fruit yield was higher with Gomera-1, while Gomera-3 gave fruit with greater weight and width. Tree height, trunk cross-sectional area, canopy diameter and volume were greater in grafted and ungrafted Gomera-3, this rootstock being the most vigorous. The yield efficiency was significantly greater on Gomera-1. Thus, cv. ‘Osteen’ grafted to Gomera-1 offered better yield and required a lower amount of nutrients.  相似文献   

Experimental Results on the Effects of Nonregular Spatial Patterns of Plants on Yield per Area     

M. Hühn 《Journal of Agronomy and Crop Science》1999,182(2):89-98

Based on several simplifying assumptions, a stochastic approach was developed which allows an estimation of the effects of nonregular spatial patterns of the distribution of individual plants on yield per area (F). In this approach, two random variables were attached to each plant: single plant yield (E) and individual space per plant (A). The latter was estimated by the area of Thiessen polygons. Yield per area was calculated theoretically by the expectation of the ratio E / A. Appropriate approximations of this expectation depend on the means (ē and ā), coefficients of variation (vE and vA) of E and A and their correlation (rEA). Yield per area can be decomposed into two additive terms: the first term gives the commonly used estimate ē/ā— or h(ā)/ā if a functional relationship between E and A is assumed: E = h(A). In this study, the two relationships E = k1 + k2 · ln A and E = A/(k3 + k4A) were used (with appropriately chosen constants k1, k2, k3, and k4). The second term in the decomposition of F can be interpreted as the effect of variable individual plant spaces on yield per area. In this paper, all theoretical concepts and results were applied to 17 experimental data sets of three cultivars of winter oilseed rape (Brassica napus L.). Single plant yields (E) and individual plant areas (A) were positively correlated with correlation coefficients from 0.64 up to 0.91. The ranges for both coefficients of variation were similar: 0.27 ≤ vE ≤ 0.65 and 0.28 ≤ vA ≤ 0.59. One obtains no significant differences in the goodness-of-fit for both tested relationships between E and A although the logarithmic relationship seems to be slightly superior. For only three data sets one obtains negative values for the percentage of the second term in the decomposition of F. This indicates an overestimation of yield per area by the commonly used estimates h(ā)/ā and ē/ā, respectively. These overestimations, however, are less than 5 %. In all other cases with positive values for the second term the yield per area is underestimated by the common estimates. For almost all data sets, however, the percentages of F which are explained by the common estimates are much larger than 90 %.  相似文献   

Irrigation Water Management for Efficient Water Use in Mulched Onion     

A. M. Abu-Awwad 《Journal of Agronomy and Crop Science》1999,183(1):1-7

Caused by the necessarily imperfect seed placement accuracy of sowing machines and, additionally, caused by many other biotic and abiotic factors, the resulting plant stands exhibit nonregular spatial distributions of its plants. Based on several simplifying assumptions, a stochastic approach is developed which allows an estimation of the effects of nonregular spatial patterns on yield per area. In this approach, two random variables are attached to each plant: single plant yield E and individual space A . The latter is estimated by the area of Thiessen polygons. Yield per area, calculated by the expectation of the ratio E/A , can be approximately expressed dependent on the means ( Ē and Ā ) and coefficients of variation ( v _E and v _A ) of E and A and their correlation ( r _EA ). In relation to the commonly used estimate Ē/Ā for yield per area, one obtains yield decreases if v _A / v _E  <  r _EA . This inequality, however, will be usually valid in the field of applications. The theoretical approaches and results were applied to three experimental data sets for drilled seeds of winter oilseed rape ( Brassica napus L.) (plant density: 60 plants m⁻², row distance: 10 cm). These data sets are characterized by different accuracies of longitudinal distributions within rows (58 %, 101 %, 150 %): yield depression increases with an increasing variability of plant distances within rows.  相似文献   

Role of Testa, Cotyledons and Embryonic Axis in Seed Dormancy of Groundnut (Arachis hypogaea L.)     

A. Bandyopadhyay  P. C. Nautiyal  T. Radhakrishnan  H. K. Gor 《Journal of Agronomy and Crop Science》1999,182(1):37-41

Experiments were carried out during winter–summer (January–June) season to understand the role of seed testa, cotyledons and embryonic axis in imparting dormancy of some groundnut cultivars belonging to different habit groups. Crop was harvested at four maturity stages; 90, 100, 110 and 121 days after emergence (DAE). After drying the pods in shade for 2 days, the germination of seeds with (GST) and without (GSW) testa in rolled germination towels and seeds, and embryonic axes (GEM) in culture media from individual plants of a cultivar was studied. Seed testa played an important role in imparting dormancy followed by the cotyledons, and embryonic axis. However, the nature of dormancy of embryonic axis appeared to be different from that of the testa and cotyledons. Results suggested that the dormancy in groundnut is regulated mainly by testa (a maternal tissue) in the Spanish type, but by cotyledons, and embryonic axis (both zygotic tissue) as well as testa in Virginia types. Thus the genetic control of seed dormancy in groundnut appears to be quantitative in nature.  相似文献   

利用RIL群体和自然群体检测与花生含油量相关的SSR标记     

黄莉  赵新燕  张文华  樊志明  任小平  廖伯寿  姜慧芳  陈玉宁 《作物学报》2011,37(11):1967-1974

以远杂9102×中花5号杂交后代衍生的重组近交系F₈代家系为材料，在含油量测试的基础上，选用10份低油材料(平均含油量52.91%)、12份高油材料(平均含油量58.85%)以及亲本进行SSR引物筛选，通过631对SSR引物扩增，筛选出来源于7对引物的13个有显著差异的片段可以有效区分低油材料和高油材料。以这7对差异引物在F₈ RIL群体中扩增，对20份低油家系材料(含油量<55%)和45份高油家系材料(含油量>56%)进行统计分析，获得1个与花生含油量相关的分子标记2A5-250/240，其中，标记2A5-250为低油材料(含油量<55%)所拥有，相符率为95.0%，标记2A5-240为高油材料(含油量>56%)所拥有，相符率为88.9%。用SSR标记2A5-250/240检测11份高油(平均含油量为55.93%)栽培种花生和11份低油(平均含油量为48.41%)栽培种花生，结果表明，标记2A5-240与高油栽培种花生的符合率为63.6%，2A5-250与低油栽培种花生的符合率为90.9%。在19份高油(平均含油量为58.60%)野生花生中，10份野生花生能检测到标记2A5-240。综合分析RIL群体和自然群体的研究结果表明，标记2A5-250/240可用于花生含油量分子标记辅助选择。  相似文献   

番木瓜组培种苗推广应用的几个关键技术问题     

周鹏  沈文涛  黎小瑛  言普 《热带作物学报》2011,32(2):354-358

重点讨论番木瓜成龄侧芽外植体污染率高、不同品种继代培养基差异较大、继代苗易发生玻璃化、组培苗诱导生根困难等关键技术问题,并对今后番木瓜组培苗推广应用作了展望。  相似文献   

Shape and distribution of knots in a sample of picea abies logs     

《Scandinavian Journal of Forest Research》2012,27(1):50-56

In an attempt to develop a better understanding of the effect of knottiness on the yield of logs for sawn timber products, the present study examines the position and geometrical characteristics of 429 knots found in seven Norway spruce (Picea abies (L.) Karst) middle logs from southern Finland. The raw data used in this study were in the form of coordinates of points located on the knot surface recorded in a cylindrical (R, L, T) reference frame. The data were smoothed using a non linear three‐dimensional model. A curvature analysis of the knot pith was used to differentiate the curved and straight portions of the knots. This analysis made possible calculation of knot angles, symmetry, ovality and volume. The major finding of this study is the uneven distribution of knots around the log circumference. Nearly 50% of the total knot volume was concentrated in one‐third of the log facing the south‐east. Knots were also more prominent in higher portions of the logs studied. These observations support the argument that the uneven distribution of knots offers potential for improving the grade yield of timber sawn from trees harvested in high‐latitude countries.  相似文献