永胜县天保工程一期建设成效评价     

施奋华 《林业调查规划》2015,40(1):115-119

介绍永胜县天保工程在森林防火、森林病虫害、生物多样性保护、公益林建设以及资金管理使用等方面的情况。分析天保工程一期实施13年来的成效,认为森林覆盖率大幅度提高,生态状况和经济社会面貌发生了显著变化,森林资源实现了恢复性增长,工程生态效益、社会效益、经济效益显著,但也存在着林牧矛盾突出,公益林建设投资不足影响后期成效,森工企业基础设施及职工生活、工作条件差、物价水平上升使得职工生活水平急剧降低等问题。藉此提出相应对策与建议。  相似文献   

花卉的花期调控   总被引：6，自引：0，他引：6  

李长征 《山西林业科技》2003,(4):25-29

利用一些特殊的栽培技术可使花卉在自然花期之外按照人的意愿定时开放，此种栽培，开花期比自然花期提早的，称为促成栽培，比自然花期延迟的称为抑制栽培。该文介绍了促成栽培和抑制栽培的调控技术，包括温度处理、光照处理和栽培技术处理等。  相似文献   

栓皮栎物候期统计分析   总被引：1，自引：0，他引：1  

张贞  王艳莉  肖庆业  丁思统 《江西林业科技》2006,(5):39-40

通过样株物候观测记录、资料利用,典范相关分析,揭示出一组花变量和一组叶变量之间高度关联。  相似文献   

MSPD-GC-MS/MS法测定竹笋中26种有机氯农药残留量     

席慧  杨振兴  张帆 《湖南林业科技》2015,(4)

以基质固相分散-气相色谱-串联质谱法(先将粉粹的竹笋样品经弗罗里硅土基质固相分散技术净化,然后用20 m L丙酮—正己烷溶液(1+2,v/v)淋洗,GC—MS/MS检测,外标法定量)检测竹笋中26种有机氯农药残留量。结果表明:26种有机氯农药在5~200μg/m L范围内线性良好,相关系数均大于0.99。在0.01~0.1 mg/kg浓度范围内,26种目标物的平均加标回收率为81.1%~108.9%,相对标准偏差在3.3%~9.5%。该方法具有较好的准确度和精密度,各项技术指标均能满足竹笋中26种有机氯农药的检测需要。  相似文献   

No impact of a phytogenic feed additive on digestion and unspecific immune reaction in piglets     

Muhl A  Liebert F 《Journal of animal physiology and animal nutrition》2007,91(9-10):426-431

Two 35 day experiments were conducted to examine the influence of a commercial phytogenic feed additive (PFA) on nutrient digestibility and unspecific immune reaction of piglets in the post-weaning period. The PFA composition was inulin, an essential oil mix (carvacrol and thymol), chestnut meal (tannins), and cellulose powder as carrier substance. In each experiment, immediately after weaning 40 male castrated piglets were divided into four experimental groups (n = 10). Diets were based on wheat, barley, soy bean meal and fishmeal using lysine as the first limiting amino acid. In experiment 1, graded levels of the PFA were supplied (A: control; B: 0.05% PFA; C: 0.1% PFA; D: 0.15% PFA). Experiment 2 utilized equal diets with 0.1% of the PFA, but different lysine supply (A: control; B: 0.1% PFA; C: +0.35% lysine; D: 0.1% PFA + 0.35% lysine). At the end of the experimental period, acute phase proteins (APPs) haptoglobin and C-reactive protein were examined in individual blood plasma samples. Following each growth study, 16 animals (n = 4) were taken for sampling of ileal chyme and assessing of praecaecal digestibility of protein and amino acids. In addition, digesta samples of the duodenum and the total pancreatic tissue were utilized for determining the enzyme activity of alpha-amylase and trypsin. APP, praecaecal digestibility and enzyme activities did not significantly respond to the PFA supplementaion in diets.  相似文献   

Acute phase proteins as indicators of calf herd health     

Gånheim C  Alenius S  Persson Waller K 《Veterinary journal (London, England : 1997)》2007,173(3):645-651

The potential for using acute phase proteins (APPs) in the assessment of herd health was studied by examining the levels of serum haptoglobin, serum amyloid A (SAA) and plasma fibrinogen in relation to clinical findings and leukocyte counts in calves. Two groups of calves from conventional dairy farms were studied. The animals were examined 10 times during the first six weeks after introduction into a new environment. Haptoglobin, SAA and fibrinogen were analysed and weight gain, disease symptoms and treatments were recorded. Analysis of antibodies against viral infections was performed. An acute phase reaction (APR) score was established at each sampling by combining the APP results and total leukocyte counts. The health status differed between the two groups, although no manipulation of health had been performed, except that the group with a higher incidence of disease had a concurrent experimental infection with lungworm as part of another study. In the group with a higher incidence of disease, the mean weight gain was significantly lower, and the number of sampling days with elevated serum concentrations of APPs, and the mean maximum concentrations of haptoglobin and fibrinogen were significantly higher compared to the healthier group. The APR score was significantly higher at days 4 and 8 of the study in the group with a higher incidence of disease. The results indicate that measurement of APPs could be a useful tool for evaluation of health in calf herds.  相似文献   

Determination phase at transition of gonocytes to spermatogonial stem cells improves establishment efficiency of spermatogonial stem cells in domestic cats     

Narong TIPTANAVATTANA  Araya RADTANAKATIKANON  Poul HYTTEL  Hanne HOLM  Supranee BURANAPRADITKUN  Piyathip SETTHAWONG  Mongkol TECHAKUMPHU  Theerawat THARASANIT 《The Journal of reproduction and development》2015,61(6):581-588

The development of germ cells has not been entirely documented in the cat especially the transition phase of the gonocyte to the spermatogonial stem cell (G/SSC). The aims of study were to examine testicular development and to identify the G/SSC transition in order to isolate and culture SSCs in vitro. Testes were divided into 3 groups according to donor age (I, < 4 months; II, 4–6 months; and III, > 6 months). In Exp. 1, we studied testicular development by histology, transmission electron microscopy and immunohistochemistry. In Exp. 2, we determined the expression of GFRα-1, DDX-4 and c-kit and performed flow cytometry. The SSCs isolated from groups II and III were characterized by RT-PCR and TEM (Exp. 3). Chronological changes in the G/SSC transition were demonstrated. The size, morphology and ultrastructure of SSCs were distinguishable from those of gonocytes. The results demonstrated that group II contained the highest numbers of SSCs per seminiferous cord/tubule (17.66 ± 2.20%) and GFRα-1⁺ cells (14.89 ± 5.66%) compared with the other groups. The findings coincided with an increased efficiency of SSC derivation in group II compared with group III (74.33 ± 2.64% vs. 23.33 ± 2.23%). The colonies expressed mRNA for GFRA1, ZBTB16, RET and POU5F1. Our study found that the G/SSC transition occurs at 4–6 months of age. This period is useful for isolation and improves the establishment efficiency of cat SSCs in vitro.  相似文献   

Serum Surfactant Protein D and Haptoglobin as Potential Biomarkers for Inflammatory Airway Disease in Horses          下载免费PDF全文

M. Bullone  M. de Lagarde  A. Vargas  J.‐P. Lavoie 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2015,29(6):1707-1711

Background

The identification of serum biomarkers of lung inflammation would facilitate the diagnosis of inflammatory airway disease (IAD) in horses.

Hypothesis

Horses with IAD have higher serum concentrations of markers of inflammation compared to controls.

Animals

Twelve horses with IAD and 10 control horses.

Methods

This was a prospective case–control study. Blood and BALF were collected from horses with IAD and controls. Serum concentration of surfactant protein D (SP‐D), haptoglobin, serum amyloid A (SAA) and of the soluble form of triggering receptor expressed on myeloid cells 1 (sTREM‐1) was measured using commercial ELISA tests.

Results

Horses with IAD had higher serum concentration (log‐transformed values) of SP‐D (mean ± SD: 1.773 ± 0.51), haptoglobin (6.657 ± 0.202) and SAA (0.128 ± 0.396) compared to controls (0.942 ± 0.226, 6.38 ± 0.22, −0.398 ± 0.319, respectively; P < .01 for all). Furthermore, the concentrations of SP‐D and haptoglobin combined allowed differentiating the 2 groups (IAD: 8.43 ± 0.564, controls: 7.322 ± 0.249, P < .0001) with a sensitivity and specificity of 100% when a cut‐off of 7.70 (log value) was employed.

Conclusions and Clinical Importance

Surfactant protein D and haptoglobin serum concentrations could be a diagnostic aid in IAD. Further studies are necessary to establish the specificity of our findings before they can be applied in everyday practice.  相似文献   

Plasma iron,C‐reactive protein,albumin, and plasma fibrinogen concentrations in dogs with systemic inflammatory response syndrome          下载免费PDF全文

Carlos Torrente DVM  MSc  PhD  Edgar G. Manzanilla DVM  PhD  MPVM  DECPHM  Luis Bosch DVM  MSc  Laura Fresno DVM  PhD  Montserrat Rivera del Alamo DVM  PhD  DECAR  Anna Andaluz DVM  PhD  Yolanda Saco DVM  PhD  Rafael Ruiz de Gopegui DVM  PhD  DECVIM 《Journal of Veterinary Emergency and Critical Care》2015,25(5):611-619

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Regional differences in the effects of various doses of cerulein upon the small-intestinal migrating motor complex in fasted and non-fasted sheep     

Romański KW 《Journal of animal physiology and animal nutrition》2007,91(1-2):29-39

There are no precise data concerning the effect of cerulein on the small-intestinal migrating motor complex (MMC) in sheep. Thus, the effort was directed towards the elucidation of the role of cerulein, a cholecystokinin (CCK) amphibian analogue, in the regulation of MMC cycle in conscious fasted and non-fasted sheep in various regions of the small intestine. In six sheep, equipped with bipolar electrodes in the entire small intestine and with one duodenal strain gauge force transducer in four of these animals, the continuous myoelectric and motor activity recordings were performed. During control period, two normal consecutive MMC cycles were recorded in fasted and non-fasted animals and then cerulein was slowly injected at the dose 1 (during 30 s), 10 (during 30 s or 60 s) or 100 ng/kg (during 30 s, 60 s or 120 s) or infused at the dose 0.5 or 1.5 ng/kg/min during 60 min. After cerulein administration at least two consecutive MMC cycles were also recorded. It was found that cerulein in moderate and higher doses injected in both fasted and non-fasted animals, especially during phase 2a or 2b MMC, inhibited phase 3 in the duodenum in at least one-third of the experiments and significantly increased the MMC cycle duration. The application of 100 ng/kg of cerulein during 120 s in the course of phase 2b MMC prolonged cycle duration from 70 +/- 21 to 113 +/- 19 min (p < 0.05) or from 51 +/- 18 to 113 +/- 19 min (p < 0.01) in fasted and non-fasted sheep respectively. The first phase 3 MMC arriving after cerulein administration at the highest dose was usually abnormal in approximately 60% of the experiments. The second phase 3 observed following the hormone injection was abnormal much less frequently and to the lesser extent. Furthermore, both cerulein doses significantly shortened the duration of phase 3 MMC either in fasted (from approximately 5.5-6.5 cpm to 4-5 cpm) or in non-fasted sheep (from approximately 4.5-5.5 to 3-4.5 cpm). The effects of cerulein exhibited the dose-response character. They were more pronounced when cerulein was injected during phase 2b or 2a MMC when compared with phase 1 MMC. The effects of cerulein on the ileal MMC were less evident, although the inhibition of the ileal phase 3 in response to both higher injected doses was observed occasionally. Infusions of cerulein in non-fasted sheep exerted similar effects as cerulein injections. It was stated that cerulein exerts marked regulatory effect upon the small-intestinal MMC in sheep. Although it remains uncertain whether the efficient doses of cerulein used in the present study correspond to physiological or pharmacological doses of CCK it seems likely that most of these doses were physiological. Thus, it is concluded that cerulein participates in the control of MMC cycles in the digestive and interdigestive states in sheep. It is possible that CCK is a physiological regulator of the ovine small-intestinal MMC.  相似文献