番鸭小肠碱性磷酸酶的分离纯化及部分性质研究     

刘建昌  余万凯  池雪林  刘姗姗 《福建农林大学学报(自然科学版)》2010,39(2)

分离纯化番鸭小肠中碱性磷酸酶,并对其酶促动力学性质进行研究.结果表明:该酶催化磷酸苯二钠水解反应时最适温度为45℃,最适pH为9.72,米氏常数(Km)为4.76 mmol.L-1.Mg2+对碱性磷酸酶活性有明显激活作用;Cd2+浓度小于0.4 mg.L-1时对碱性磷酸酶有激活作用,而浓度高于0.4 mg.L-1时则有抑制作用;Cu2+对碱性磷酸酶活性有明显抑制作用.  相似文献   

鹅源H5N1禽流感病毒感染对雏鸭红细胞免疫功能的影响     

郑世民  葛依阳  马宏伟  高雪丽  刘超男  吕晓萍 《东北农业大学学报》2019,50(1):45-51

将100只21日龄雏鸭随机分为AIV感染组(I)和对照组(C),应用免疫学和细胞培养及MTT等方法动态检测AIV感染雏鸭血液红细胞免疫黏附能力、红细胞免疫自身调控能力以及红细胞促外周血淋巴细胞增殖能力。结果表明,雏鸭感染AIV后1～14 d,红细胞C_(3b)受体花环(E-CRR)率明显低于对照组,而红细胞免疫复合物花环(E-ICR)率明显高于对照组;AIV感染雏鸭后3～21 d,红细胞C_(3b)受体花环促进率(RFER)显著低于对照组,且红细胞C_(3b)受体花环抑制(RFIR)率显著高于对照组;AIV感染雏鸭的红细胞对血液T、B淋巴细胞增殖促进能力明显低于对照组。表明AIV感染引起的雏鸭红细胞免疫黏附功能、自身调控能力和T、B淋巴细胞增殖能力下降,与AIV感染导致雏鸭外周血免疫功能降低密切相关。  相似文献   

大豆黄酮对白羽番鸭繁殖性能及相关激素水平的影响     

张响英  唐现文  刘海霞  朱淑斌 《家畜生态》2008,(6):43-45

在基础日粮中分别添加6mg/kg和12mg/kg大豆黄酮饲喂44周龄的白羽番鸭。结果发现：6mg/kg大豆黄酮组番鸭产蛋率显著提高（P〈0.01）,血清孕酮和T3水平上升（P〈0.01）,而12mg/kg大豆黄酮组番鸭产蛋率呈下降趋势;两试验组种蛋受精率和孵化率均明显下降。试验结果表明：大豆黄酮可通过调节体内激素分泌,进而影响番鸭的生殖功能。  相似文献   

Effects of Fermented Peanut Vine on Production Performance,Slaughter Performance,Serum Biochemical Indexes and Feces' Routine Ingredients in Muscovy Ducks     

LIU Zhenni  LEI Xiaowen  KONG Zhiwei  ZHONG Yunping  XIE Hualiang  TAN Donghai  WU Yijian 《中国畜牧兽医》2007,47(11):3493-3501

The aim of this study was to explore the nutritional value of fermented peanut vine and its effects on the production performance,slaughter performance,serum biochemical indexes and feces' routine ingredient in Muscovy ducks.The feasibility of fermented peanut vine that applied to grain-saving feeding in Muscovy ducks was also explored.Three hundred and sixty 15-day-old male Muscovy ducks were randomly divided into 4 groups,6 repetitions in each group,15 ducks each repetition.The ducks in control group were fed with basic diet,while in group Ⅰ were fed with 90% basic diet+10% fermented peanut vine,in group Ⅱ were fed with 85% basic diet+15% fermented peanut vine and in group Ⅲ were fed with 80% basic diet+20% fermented peanut vine.After 7 days pre-feeding period and 48 days feeding period,production performance,slaughter performance and organ indexes were then measured.Meanwhile,serum,muscle and fresh feces were collected for measurement of the levels of biochemical indexes and feces'routine ingredients.The results showed that:① Compared to unfermented peanut vine,CP and Ca levels of fermented peanut vine were elevated by 31.82% (P<0.01) and 97.40% (P<0.01) respectively,while CF,P and H₂O levels were decreased by 2.35% (P<0.05),20.69% (P<0.05) and 53.23% (P<0.01) respectively.Nutritional value of fermented peanut vine was clearly increased.② Compared to control group, F/G of group Ⅰ and Ⅱ were decreased by 6.51% (P<0.01) and 6.84% (P<0.01) respectively.The leg muscle rate of groupⅠ,Ⅱ and Ⅲ were improved by 20.50% (P<0.05),25.55% (P<0.05) and 9.27% (P>0.05) respectively,and liver indexes were increased by 9.90% (P>0.05)、12.23% (P<0.05) and 3.18% (P>0.05) respectively,gizzard indexes were elevated by 17.70% (P<0.05),30.82%(P<0.05) and 36.66% (P<0.05) respectively,and glandular stomach indexes were increased by 1.37% (P>0.05),32.99% (P<0.05) and 36.43% (P<0.05) respectively.③ Fermented peanut vine could affect the levels of TP,ALB,GLB,A/G,ALT,AST,AST/ALT,UREA,UA and CRE in serum of Muscovy ducks.Compared to control group,N,P,K and H₂O levels in feces of ducks were decreased in the test groups.In conclusion,10% and 20% fermented peanut vine could markedly improve the production performance,reduce cost to gain ratio,and did not affect meat performance.But fermented peanut vine might affect the function of liver,kidney and immune in Muscovy ducks.  相似文献   

Isolation,Identification and Drug Resistance Analysis of Riemerella anatipestifer from Muscovy Duck     

XIAO Kun  CHEN Guang  ZHANG Jun  WEN Guilan  WEN Ming  CHENG Zhentao 《中国畜牧兽医》2007,47(12):4085-4092

In order to understand the drug resistance of Riemerella anatipestifer from Muscovy duck,this study carried out bacterial isolation and culture,Gram staining microscopy,pathogen detection,biochemical test,16S rRNA sequence analysis,PCR identification,drug sensitivity test and drug resistance gene detection for Muscovy duck suspected of Riemerella anatipestifer infection.The results of bacterial isolation showed that on the blood agar medium,the isolated bacteria grew creamy needle tip size colonies with smooth surface,neat edge,luster and translucency.The Gram-negative bacillus brevis was detected by Gram-negative staining microscopy,and it was named GZQN201907.In the biochemical test of GZQN201907,urea reaction was positive,but glucose,maltose,lactose and other biochemical reactions were negative.The 16S rRNA phylogenetic tree was in the same branch as Riemerella anatipestifer.And the OmpA gene of PCR identification results of the isolated strain were positive.The drug sensitivity test was sensitive to 18 antibiotics including cefuroxime,erythromycin and ceftazidime,moderately sensitive to carboxypicillin and ciprofloxacin,and sensitive to neomycin and cotrimoxazole.And the resistance genes could detect the β-lactam resistance genes VIM and TEM,tetracycline resistance genes tetB,macrolide resistance genes ermB and ermF.The results of drug sensitivity test and drug resistance gene detection indicated that GZQN201907 showed the same resistance phenotype and gene detection results for β-lactam,tetracycline and macrolide.In the animal regression test,all the ducklings inoculated with GZQN201907 died within 72 h,while the control group showed no symptoms,indicating that GZQN201907 was virulent to the ducklings.One strain of Riemerella anatipestifer from Muscovy duck was successfully isolated,which laid a foundation for the prevention and treatment of Riemerella anatipestifer from muscovich.  相似文献   

仔鸭实验性食盐缺乏症的症状和病理变化观察     

许美解  陈可毅 《湖南畜牧兽医》1997,(4):11-13

用含食盐量不同的全价日粮在自由饮水条件下，对樱桃谷雏鸭从1日龄起进行了喂饲试验．结果表明，喂饲不加盐日粮的雏鸭在7～18日龄期间全部发病死亡，出现典型的食盐缺乏症病状和病理变化．喂饲0．15％低盐日粮的雏鸭则表现食欲不振，生长发育较慢，但无死亡．  相似文献   

雏番鸭细小病毒病诊断技术和试剂的研究   总被引：8，自引：1，他引：7  

程由铨  胡奇林 《中国兽医学报》1997,17(5):434-436

根据番鸭细小病毒单克隆抗体（ＭＰＶ－ＭｃＡｂ）致敏的胶乳可与ＭＰＶ产生特异性凝集反应，而这种特异性凝集反应可被ＭＰＶ抗体所抑制的原理，建立了胶乳凝集试验（ＬＰＡ）和胶乳凝集抑制试验（ＬＰＡＩ）。致敏抗体蛋白浓度为０．５ｇ／Ｌ时，ＬＰＡ可检测出ＭＰＶ最小蛋白量为２μｇ／Ｌ。对ＬＰＡ与ＦＡ、ＬＰＡＩ与ＡＧＰ作了比较，结果：ＬＰＡ阳性率９０．５％，ＦＡ阳性率９２．４％，两者的符合率９２．４％；鸭血清ＬＰＡＩ抗体效价在Ｌｏｇ２５以下时，ＡＧＰ试验均阴性，前者比后者敏感性高３２～６４倍。ＬＰＡＩ抗体效价与保护力相关性试验结果表明，当鸭血清中ＬＰＡＩ抗体效价在Ｌｏｇ２１以上时，雏番鸭均能耐受强毒的攻击，两者呈正相关。致敏胶乳和抗原保存时间在４～８℃时，分别为５个月和６个月；在２２～２８℃时，分别为７ｄ和１５ｄ。  相似文献   

黑羽番鸭LPL基因克隆及在肌肉发育中的表达规律     

王丽华  段修军  董飚  孙国波  卞友庆 《安徽农业大学学报》2014,41(3):385

脂蛋白脂酶(LPL)是水解甘油三酯的关键性酶,可以为细胞提供游离的甘油三酯,并作用于脂蛋白循环,与机体的脂质代谢有密切关系。本研究利用同源克隆策略克隆黑羽番鸭LPL基因,并采用实时荧光定量PCR技术分析其在肌肉组织发育过程中mRNA表达规律。结果表明,黑羽番鸭LPL基因cDNA序列长度为1515 bp,包括了1485 bp的编码区,编码494个氨基酸。与禽类LPL基因核苷酸和氨基酸序列同源性均在90%以上,与人和哺乳动物同源性在70%~75%之间。实时荧光定量PCR检测结果显示,LPL基因在第2周龄胸肌中mRNA表达量较低,然后开始上升,达到最大值再出现下降并保持在一定水平上;公鸭腿肌表达规律和公鸭一致,但是母鸭腿肌表达量呈波浪状。  相似文献   

Quantitative transmission characteristics of different H5 low pathogenic avian influenza viruses in Muscovy ducks     

Éric Niqueux  Jean-Paul Picault  Michel Amelot  Chantal Allée  Josiane Lamandé  Carole Guillemoto  Isabelle Pierre  Pascale Massin  Guillaume Blot  François-Xavier Briand  Nicolas Rose  Véronique Jestin 《Veterinary microbiology》2014

EU annual serosurveillance programs show that domestic duck flocks have the highest seroprevalence of H5 antibodies, demonstrating the circulation of notifiable avian influenza virus (AIV) according to OIE, likely low pathogenic (LP). Therefore, transmission characteristics of LPAIV within these flocks can help to understand virus circulation and possible risk of propagation. This study aimed at estimating transmission parameters of four H5 LPAIV (three field strains from French poultry and decoy ducks, and one clonal reverse-genetics strain derived from one of the former), using a SIR model to analyze data from experimental infections in SPF Muscovy ducks. The design was set up to accommodate rearing on wood shavings with a low density of 1.6 ducks/m²: 10 inoculated ducks were housed together with 15 contact-exposed ducks. Infection was monitored by RNA detection on oropharyngeal and cloacal swabs using real-time RT-PCR with a cutoff corresponding to 2–7 EID50. Depending on the strain, the basic reproduction number (R₀) varied from 5.5 to 42.7, confirming LPAIV could easily be transmitted to susceptible Muscovy ducks. The lowest R₀ estimate was obtained for a H5N3 field strain, due to lower values of transmission rate and duration of infectious period, whereas reverse-genetics derived H5N1 strain had the highest R₀. Frequency and intensity of clinical signs were also variable between strains, but apparently not associated with longer infectious periods. Further comparisons of quantitative transmission parameters may help to identify relevant viral genetic markers for early detection of potentially more virulent strains during surveillance of LPAIV.  相似文献   

Effect of semen extenders and storage time on quality of Muscovy duck (Cairina moschata) drake semen during the entire reproductive season     

Ewa Łukaszewicz  Anna Jerysz  Bronisława Chełmońska 《Reproduction in domestic animals》2020,55(8):943-950

Avian semen dilution with appropriate extender allows to prolong the fertilizing ability of sperm stored in vitro. In the present study, the impact of extenders and time of storage on morphology of Muscovy duck (Cairina moschata) drake semen were examined. Semen was collected twice a week, using male stimulation by a female method, from 12 adults (29 weeks old) drakes kept individually in cages, under controlled environmental conditions. Freshly collected, pooled ejaculates were divided into three part: neat undiluted sample, and diluted 1:1 with Schramm (SCH) or Watanabe (W) extender and stored at 4°C. Morphological examination of all samples was conducted after dilution and then, after 3 and 6 hr of storage. The storage of undiluted semen caused decrease (p ≤ .01) in live morphologically normal sperm, from 79.73% in the freshly collected ejaculates to 55.75% and to 12.12% after 3 and 6 hr of storage, respectively (average calculated for the entire reproductive season). In the semen diluted with Schramm's extender the adequate values attained 86.84, 79.65 and 61.66%, and using Watanabe extender 84.77, 83.58 and 75.25%, respectively. The period of semen storage and the type of extender caused significant (p ≤ 0,05; p ≤ 0,01) changes in sperm morphology. The longer period of storage contributed to the decrease in number of morphologically normal sperm, whereas their content in Watanabe extender after 3 and 6 hr of storage was higher (p ≤ .01) than in semen diluted in Schramm extender.  相似文献