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41.
LIAO Guo-ning LI Qing-fen FENG You-mei DENG Yao-zu LI Zhuo-ya GONG Fei-li MA Ding 《园艺学报》2004,20(1):38-41
AIM: To inhibit specifically the u-PAR expression in highly invasive cell subclones and block its function in those cells invasion. METHODS: A cDNA fragment of u-PAR obtained by RT-PCR was inserted into a plasmid vector named pcDNA3 in the reverse direction. Then an antisense RNA expression plasmid for u-PAR was constructed and transfected into highly invasive cell subclones. The u-PAR expression in resistant cells was examined by RT-PCR and immunohistochemical assay. RESULTS: Compared to the control cells, the content of mRNA and protein of u-PAR in transfected cells decreased sharply, and the rate of inhibition was 53% and 73%, respectively. CONCLUSION: The results indicated that an antisense vector for u-PAR might played a specific inhibitory role in the cells. This model is useful for observing the inhibitory effects of the antisense vector for u-PAR on invasion by highly invasive cell subclones of human prostate carcinoma. 相似文献
42.
AIM: To investigate the physiological function of the novel serine protease SNC19 protein and its possible role in cancer invasion and metastasis. METHODS: Monoclonal antibodies directly against SNC19 extracellular domain was prepared. The protein and SNC19 mRNA expression were determined in different kinds of cell lines respectively by Western blot and Northern blot analysis. Cellular migration and adhesion abilities were assayed by monoclonal antibody blocking method. RESULTS: Western blot analysis showed there were two bands of SNC19 protein in BCAP37, COLO205, SW480 cells at about 120 kD and 60 kD while only one band in SW620 cells at 60 kD; Northern blots showed a approximate 3.4-kilobase fragment appearing in most epithelial-derived cell lines with this only form and high levels but no detection was obtained in OV, TCA8113, KB and SGC7901 cells. In antibody blocking experiments, the migration of SW480 cells was significantly inhibited compared with the control and the abilities of SW480/SW480, SW480/NIH3T3 adhesion increased at the beginning of the experiments, but the difference reduced along with the time passed.CONCLUSION: SNC19 protein is closely related with cellular homogeneous and heterogeneous adhesion as well as cellular motility. As a novel serine protease, it may participate both in physiological and pathological processes, such as cell migration, tissue remodeling and cancer invasion and metastasis. 相似文献
43.
HE Qiang HUANG Mei-jin PENG Bao-gang LIANG Li-jian SHEN Shun-li ZHOU Fan 《园艺学报》2007,23(12):2369-2373
AIM: To explore whether or not ErbB2-induced oncogenic transformation and invasion are mediated by FAK.METHODS: Parental FAK-/- and FAK+/+ cells were used and infected with retrovector particles expressing ErbB2 in order to acquire ErbB2-overexpressed cells,i.e.,FAK-/--ErbB2 and FAK+/+-ErbB2.The role of FAK signaling was explored by analyzing the parameters such as cell survival,invasiveness and tumorigenicity shown by both FAK-/- and FAK+/+ cells in which ErbB2 was overexpressed.RESULTS: ErbB2 was overexpressed and functionally activated in both FAK-/- and FAK+/+ cells upon infection with retrovector particles.The ErbB2-induced anchorage-dependent cell survival,cell invasiveness as well as tumorigenicity in vivo were dependent on FAK.CONCLUSION: FAK is essential for cell survival,tumorigenicity and invasiveness induced by ErbB2,and its possible mechanism involves in ErbB-FAK-Src-MAPK pathway. 相似文献
44.
LIU Jian-hua HUANG Kai-hong LI Xue-xian SONG Li-bing GUO Bao-hong FENG Yan ZENG Mu-sheng 《园艺学报》2007,23(10):2018-2022
AIM: To investigate the relationship between expression of Bmi-1 (B cell-specific MLV integration site-1) in gastric cancer and its clinicopathologic significance.METHODS: 146 surgical patients with gastric carcinoma were followed up at least 2 years.Expression of Bmi-1 protein was examined by immunohistochemistry in their archival paraffin embedded tissue specimens.RESULTS: The intensive positive rate of Bmi-1 expression in gastric cancer was 67.8% (99/146).Expression of Bmi-1 was highly correlated with tumor size,clinical stage,lymph node metastasis and T classification (P<0.05),but not with sex,age,tumor differentiation,etc.(P>0.05).The survival rate in the patients with Bmi-1 expression was much lower than that in those patients without Bmi-1 expression (P<0.01).Multivariate analysis indicated that Bmi-1 expression,T classification,lymph node metastasis,distant metastasis,tumor size and postoperative chemotherapy were all significantly prognostic factors of gastric carcinoma.CONCLUSION: Overexpression of Bmi-1 in patients with gastric carcinoma enhances the possibility of invasion and metastasis,implying a poor prognosis.Bmi-1 may serve as fairly a good prognostic factor to indicate biologic behavior and prognosis in gastric carcinoma. 相似文献
45.
AIM: To investigate the angiogenesis status,the expression of vascular endothelial growth factor (VEGF),angiopoietin-1 (ANG-1),angiopoietin-2 (ANG-2),thrombospondin-1 (TSP-1) in cholangiocellular carcinoma (CCC) and relationship with tumor angiogenesis,differentiation,invasion and metastasis.METHODS: 33 specimen of surgically resected CCC were investigated.Immunohistochemical staining of CD34,VEGF,ANG-1,ANG-2 and TSP-1 was carried out.RESULTS: The mean MVD was (87.2±52.6)/mm2.VEGF positive expression was found in 75.6% cases;ANG-1 positive expression was observed in 36% cases;ANG-2 positive was detected in 57.6% cases and 45.5% cases exhibited positive TSP-1 expression.VEGF and ANG-2 expressions were found to be associated with significant higher level of MVD (P<0.01 and P<0.05,respectively).TSP-1 expression was found to be associated with significant low level of MVD (P<0.01).Positive TSP-1 expression was also found to be associated with higher level of intrahepatic metastasis (46.7% vs 5.6%,P<0.05).CONCLUSION: Considerable angiogenesis compared to other solid tumors can be observed in CCC.VEGF and ANG-2 might play a proangiogenic role and TSP-1 may play an inhibitory role.Although TSP-1 may increase the intrahepatic metastasis of CCC,neither MVD levels nor the expression of VEGF,ANG-1,or ANG-2 is associated with tumor differentiation,invasion and metastasis. 相似文献
46.
AIM:To Study on the expressive levels of PTTG and FHIT and detect their clinicopathological significances in gastric cancer and pericancerous tissues.METHODS:Immunohistochemical method was used on routinely paraffin-embedded sections of 49 cases with gastric cancer and 20 subjects with pericancerous tissues. RESULTS:The positive rate and score of PTTG in gastric cancer were significantly higher than those in pericancerous tissues (P<0.05,P<0.01),but those of FHIT were opposite (P<0.05,P<0.01),the positive cases of PTTG and negative subjects of FHIT showed severely-atypical hyperplasis. The positive rate and score of PTTG were significantly lower in the cases of infiltrating depth T1-T2 and without-metastasis of distant organs than those in the subjects of infiltrating depth T3-T4 and with-metastasis of distant organs(P<0.05,P<0.01). The positive rate and score of FHIT were significantly higher in the cases of infiltrating depth T1-T2,without-metastasis of lymphnodes,with-metastasis of the first site lymphnodes,and without metatstasis of distant organs than those in the subjects of infiltrating depth T3-T4,with-metastasis of lymphnodes,with-metastasis of the third site lymphnodes,and with-metastasis of distant organs(P<0.05,P<0.01). The significantly negative correlation was found between the score of PTTG and FHIT in gastric cancer tissues(r=-0.36,P<0.01). CONCLUSION:The expression of PTTG and FHIT might be important biological markers for reflecting the carcinogenesis,progression,invasive potential,metastastic status and prognosis of gastric cancer. The assays of PTTG and FHIT in benign lesions might have clinical values for the prevention and early-stage finding of gastric cancer. 相似文献
47.
de Freitas Campos C Cole N Van Dyk D Walsh BJ Diakos P Almeida D Torrecilhas A Laus JL Willcox MD 《Research in veterinary science》2008,85(2):349-352
The first reference map of the proteome of pooled normal dog tears was created using 2-dimensional polyacrylamide gel electrophoresis and the identity of a number of the major species determined using matrix-assisted laser desorption time of flight mass spectrometry (MALDI-TOF) and peptide mass fingerprint matching on protein sequence databases. In order to understand the changes in protein expression in the tear film of dogs with cancer, tears from such animals were similarly examined. A number of differences were found between the tears of healthy dogs and the dogs with cancer. Differences were found in levels of actin and albumin and in an unidentified protein which may be analogous to human lacryglobulin. These findings suggest that it may be possible to develop tear film analysis to provide a simple non-invasive test for the diagnosis and/or management of canine cancers. 相似文献
48.
AIM: To investigate the expression of long non-coding RNA PVT1 in ovarian cancer and the role of PVT1 in migration and invasion abilities of ovarian cancer cells.METHODS: The expression of PVT1 in ovarian cancer tissue, normal ovarian tissue and different ovarian cancer cell lines was detected by qPCR. Transwell assay was used to detect the invasion ability of ovarian cancer cells after PVT1 silencing. The migration ability of the ovarian cancer cells after PVT1 silencing was detected by scratch test. The interaction between PVT1 and microRNA (miR)-551 was analyzed by dual-luciferase reporter assay. The effect of miR-551-inhibitor on the invasion and migration abilities of ovarian cancer cells after PVT1 silencing was detected by Transwell assay and scratch test. The expression of Wnt signaling pathway-related proteins was determined by Western blot after PVT1 silencing. The effects of PVT1 silencing on tumor weight and volume of ovarian cancer were examined by subcutaneous tumor transplantation in nude mice.RESULTS: The expression of PVT1 in ovarian cancer tissue was significantly higher than that in normal ovarian tissue (P<0.05). The expression level of PVT1 in ovarian cancer cell line ES-2 was the highest. PVT1 silencing inhibited the invasion and migration abilities of the ovarian cancer cells. After PVT1 silencing, miR-551-inhibitor promoted the invasion and migration abilities of the ovarian cancer cells. The expression of Wnt signaling pathway-related proteins was decreased after PVT1 silencing (P<0.05). Compared with negative control group, the tumor volume and weight in PVT1-siRNA group were significantly decreased (P<0.05).CONCLUSION: PVT1 plays an important role in the development of ovarian cancer. PVT1 regulates the invasion and migration abilities of ovarian cancer cells through Wnt signaling pathway. 相似文献
49.
WANG Zhao-yan YANG Yu-ling YANG Zhi-yi YIN Chong-gao LI Hong-li LIU Yu-qing 《园艺学报》2018,34(6):1008-1013
AIM: To investigate the function of microRNA-125a-5p (miR-125a-5p) on epithelial-mesenchymal transition (EMT) of breast cancer cells via GSK-3β/Snail signaling pathway.METHODS: The expression of miR-125a-5p in normal breast epithelial cells and breast cancer cells, as well as the transfection efficiency of miR-125a-5p plasmid in MDA-MB-231 cells was detected by RT-qPCR. The chemotaxis ability and invasion ability were detected by chemotaxis assay and Transwell invasion assay. The changes of EMT-related markers, the protein level of phosphorylated glycogen synthase kinase-3β (p-GSK-3β) and the nuclear translocation of Snail were determined by Western blot. RESULTS: The expression of miR-125a-5p in the breast cancer cells was significantly lower than that in the normal breast epithelial cells. The expression of miR-125a-5p was significantly higher in MDA-MB-231/miR-125a-5p cells than that in MDA-MB-231/NC cells. The ability of epithelial growth factor (EGF) at 10 μg/L to induce chemotaxis of MDA-MB-231 cells was the strongest. Compared with MDA-MB-231/NC group, stimulation of EGF decreased the invasion ability of MDA-MB-231/miR-125a-5p cells, and resulted in the increase in E-cadherin expression, while significantly decreased the protein levels of vimentin and p-GSK-3β. Meanwhile, the nuclear localization of Snail was significantly inhibited. The invasion capacity of MDA-MB-231/miR-125a-5p+GAB2 cells was significantly enhanced compared with MDA-MB-231/miR-125a-5p+Con cells, the expression of E-cadherin was decreased, and the protein levels of vimentin and p-GSK-3β were significantly increased, while the nuclear localization of Snail was promoted. CONCLUSION: miR-125a-5p suppresses EMT via GSK-3β/Snail signaling pathway, thus inhibiting the invasion ability of breast cancer cells. 相似文献
50.
AIM: To construct the shRNA targeting anterior gradient protein 2 (AGR2) gene for exploring the effect of AGR2 on the biological behavior of nasopharyngeal carcinoma (NPC) cells.METHODS: The expression of AGR2 at mRNA and protein levels in NPC cell lines 6-10B and 5-8F was detected by real-time PCR and Western blot. The pSR-GFP/Neo-AGR2-shRNA expression vector targeting AGR2 was constructed. Based on the interference targeting AGR2, the cell migration and motility were determined by Transwell migration and motility assays.RESULTS: The expression of AGR2 was increased in NPC cell line 5-8F compared with NPC cell line 6-10B (P<0.05). When the AGR2 expression in 5-8F cells was interfered, the cell migration, invasion and tumorigenicity were weakened.CONCLUSION: The expression of AGR2 is up-regulated in NPC cell line 5-8F. pSR-GFP/Neo-CLU-shRNA successfully inhibits the expression of AGR2 in NPC cell line 5-8F. AGR2 inhibits the migration, invasion and tumorigenicity of 5-8F cells in vivo. 相似文献