排序方式： 共有45条查询结果，搜索用时 15 毫秒
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[目的]在自发性II型糖尿病小鼠（db／db）基础上建立1种II型糖尿病肾病小鼠模型,为糖尿病肾病新药研发提供高效优质的服务平台。[方法]用II型糖尿病小鼠（db／db）,分别在其4周龄和8周龄进行左侧单肾切除手术．待其康复1周后,再将动物按周龄大小分成2组,另外2组对应周龄的db／db小鼠进行假手术,作为阴性对照组。每2周测定空腹6小时血糖浓度和体重,每4周收集尿液,测定24h微量尿白蛋白总量。[结果]8周龄阴性对照组小鼠与其对应进行单侧肾切除的小鼠空腹6h血糖和体重并无明显差异,但24h微量尿白蛋白总量显著增高,4周龄阴性对照组小鼠与其对应进行单侧肾切除的小鼠相比,其空腹6h血糖和24h微量尿白蛋白总量并无显著增高。[结论]在db／db小鼠8周龄进行单侧肾脏切除可以加速db／db小鼠糖尿病肾病的发生和发展。 相似文献
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AIM To observe the effect of curcumin （Cur） on lupus nephritis （LN） and its possible mechanism. METHODS Thirty 10-week-old MRL/lpr lupus mice were randomly divided into MRL/lpr group， Cur-L and Cur-H group with 10 mice in each group， and C57BL/6 mice （n =10） served as normal control （NC） group. The mice in Cur-L group and Cur-H group were given intragastric administration of Cur at 100 and 200 mg·kg-1·d-1 for 12 weeks， respectively， and the same volume of normal saline was given to the mice in NC group and MRL/lpr group. The urine protein was detected， and the morphological changes of the renal tissue were observed by HE staining after treatment. The levels of serum creatinine （SCr）， blood urea nitrogen （BUN） and serum anti-double-stranded DNA （dsDNA）， and interleukin-1β （IL-1β）， IL-6 and tumor necrosis factor-α （TNF-α） levels in serum and renal tissues were detected. The protein levels of p-IκB， NF-κB， NLRP3 and caspase-1 in the renal tissues were determined by Western blot. RESULTS Compared with MRL/lpr group， the content of urine protein in Cur groups was significantly reduced， and the renal injury was relieved. The SCr， BUN， serum anti-dsDNA， and the serum and renal levels of IL-1， IL-6 and TNF-α were all significantly reduced， and the protein levels of p-IκB， NF-κB， NLRP and caspase-1 in the renal tissue were significantly decreased （P <0.05）. CONCLUSION Cur has a certain protective effect on the kidney of MRL/lpr mice， and its mechanism may be related to the inhibition of NF-κB and NLRP3 signaling pathways. 相似文献
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Miyazaki M Soeta S Yamagishi N Taira H Suzuki A Yamashita T 《Research in veterinary science》2007,82(1):76-79
Cauxin, a member of mammalian carboxylesterases (EC 3.1.1.1), is excreted as a major urinary protein in the domestic cat. Urinary cauxin is derived from the kidney proximal straight tubules. Here, we report changes in the renal expression and urinary excretion of cauxin in cats with tubulointerstitial nephritis (TIN). Immunohistochemistry using anti-cauxin antibody showed fewer cauxin-positive tubules in 15 TIN cases than in normal animals. In areas with tubulointerstitial damage, fibroblasts and inflammatory cells replaced renal tubules, and cauxin-positive tubules consequently disappeared. Urine was analysed in six of the 15 cases. In the two cases with mild tubulointerstitial changes, urinary cauxin was detected using SDS-PAGE with Coomassie staining. In the four cases with severe tubulointerstitial changes, urinary cauxin was below the detection limit using Western blotting. These results indicate that the renal expression and urinary excretion of cauxin decrease with the progression of TIN in cats. 相似文献
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