Periplaneta americana peptide decreases apoptosis of pig-ovary granulosa cells induced by H2O2 through FoxO1     

Caihua Kong  Kena Liu  Qin Wang  Rong Fu  Huaxin Si  Shiyan Sui 《Reproduction in domestic animals》2021,56(11):1413-1424

Oxidative stress can induce apoptosis of granulosa cells and lead to follicular atresia, thereby reducing the number of pigs giving birth. The aim of this study was to investigate the protective effect of Periplaneta americana peptide (PAP) on the apoptosis of the granulosa cells of pig ovaries (PGCs) induced by hydrogen peroxide (H₂O₂) via FoxO1. PGCs were treated with H₂O₂ to establish a cell apoptosis model. Cell viability was measured using the cell counting kit-8 (CCK-8) assay, and cell apoptosis was detected using flow cytometry. The malondialdehyde (MDA) level and nitric oxide (NO) content were detected to reflect the oxidative stress. Western blotting, qRT-PCR and overexpression were undertaken to determine the expression of FoxO1 and caspase-3, and immunofluorescence was used to detect FoxO1 in the nucleus and cytoplasm. PGCs were treated with 100 μM H₂O₂ for 6 hr, which resulted in oxidative damage and apoptosis and an apoptosis rate for PGCs of 32.95%. Next, PGCs were treated with 400 μg/ml PAP for 24 hr to repair the apoptosis induced by H₂O₂. PAP improved cell viability in H₂O₂-stimulated PGCs, the increased MDA level and NO content caused by H₂O₂ stimulation were reversed and the apoptotic rate of PGCs was reduced. The qRT-PCR and Western blotting results indicated that PAP decreased the H₂O₂-induced apoptosis and the expression of FoxO1 and caspase-3 in PGCs. The effect of PAP was the same following FoxO1 overexpression. FoxO1 was expressed in the nucleus when stimulated by H₂O₂ or overexpression; however, it migrated to the cytoplasm following PAP treatment. PAP decreased the apoptosis of PGCs induced by H₂O₂ by regulating FoxO1 expression and nuclear translocation.  相似文献   

纳米Al_2O_3改性水性木器漆耐磨性和硬度的研究     

龙玲  万祥龙  曲岩春 《林业科学》2011,47(9)

为提高水性聚丙烯酸酯木器漆的耐磨性和硬度,进行纳米Al2O3浆料的制备及其对水性木器漆改性的研究,探讨分析不同制备方法和Al2O3添加量对漆膜耐磨性和硬度的影响。结果表明:以聚丙烯酰胺为分散剂、聚乙二醇和辛基酚聚氧乙烯醚为润湿剂、聚乙烯吡咯烷酮为稳定保护胶,在高速搅拌下制成的纳米Al2O3浆料,在透射电镜下观察发现纳米Al2O3颗粒分布均匀,具有良好的分散性;在不影响漆膜透明度的情况下,漆膜耐磨性和硬度随Al2O3添加量增加得到提高,添加量在1.5%时漆膜具有较好的耐磨性和硬度;纳米杂合工艺法的漆膜耐磨性和硬度优于后添加法制备的水性木器漆涂膜。  相似文献   

国内外致泻大肠埃希氏菌O157:H7的检测标准比较及建议     

孟静  钟立霞  郭颖慧  程祥龙  伊廷存  霍胜楠 《中国乳业》2023,(4):76-81

致泻大肠埃希氏菌O157:H7（STEC O157:H7）是大肠埃希氏菌中致病性最严重的一种食源性致病菌,主要存在于牛肉、牛奶、水果及其制品中,对身体健康造成很大危害,甚至引发死亡。食品中STEC O157:H7检测尤为重要。本文对国内外STEC O157:H7的检测标准进行比较,提出我国标准在样品前处理、快速筛选方法的应用等方面需要加强,以便为该菌快速准确检测提供帮助,实现与国际标准化体系建设接轨,满足实验室检测需要。  相似文献   

Effect of H2O2 in the Bioremediation of Petroleum Hydrocarbon Pollutant     

HUO Dan-qun  YAO Wei-jin 《保鲜与加工》2007,(10):130-133

The high efficient strain was separated and filtrated from the soil contaminated by petroleum hydrocarbon. The authors, we focus on the effect of H2 O2 in the bioremediation of petroleum hydrocarbon pollutant. The results show that the optimal concentration of H2O2was 200 mg/L at one time. At the lag phase of the strain, H2O2 was added in per eight hour, and at logarithmic phase, H2O2 was added in per two hour. Biodegradation rate of petroleum hydrocarbon was increased from 38. 1% to 83. 1%. The bioremediation of petroleum hydrocarbon pollutant is remarkably by double effects of deep oxidation and oxygen supply of H2O2.  相似文献   

Preparation of V2O5 Semiconductor Thin Films From Induststrial V2O5     

YANG Shao li  XU Chu shao  CHEN Hou Sheng  HU Zai yong 《保鲜与加工》2002,(5):97-100

  相似文献   

ZrO_2对Al_2O_3基泡沫陶瓷过滤器的性能影响   总被引：1，自引：0，他引：1  

任凤章  王哲  李锋军  刘平  马战红  贾淑果  田保红 《拖拉机与农用运输车》2007,34(3):28-29,32

以Al2O3为主要成分的料浆中加入ZrO2,采用有机泡沫浸渍法,用1560℃烧结制备Al2O3基泡沫陶瓷过滤器,并测定不同ZrO2含量过滤器样品的抗压强度、热震次数并观察其显微结构,实验结果表明:Al2O3基泡沫陶瓷的抗压强度、抗热震性能随ZrO2加入量的增加先增加,而后略有下降。在ZrO2加入量为20%(重量)时制品的抗压强度及抗热震性能最佳,其最高抗压强度达2.31 MPa,1100℃热震循环次数为16次。  相似文献   

The rehabilitation of an irrigation system along the Yellow River     

Puli Lou  Linxiang Hou 《Irrigation and Drainage Systems》1988,2(1):9-19

The People's Victory Irrigation System which diverts water from the Yellow River of China covers a total irrigable area of 59 000 ha. The system encountered some serious problems in the first decade of its operation — salinity and waterlogging of irrigated land, siltation of irrigation and drainage channels, as well as a low efficiency of water use. This paper describes a series of structural and functional measures which have been adopted in the past 24 years for the rehabilitation of the system, including: improvement of the existing drainage system and construction of new drainage system on agricultural land, conjunctive use of surface and groundwater supplies, a comprehensive programme to reduce the levels of siltation as well as the implementation of improved water management practices. A model for the optimal operation of the system by using system analysis theory as an aid in reducing the operation and maintenance (O & M) costs has been developed in recent years. The results are that the soil salinity has been controlled and the agricultural production has increased whilst the efficiency of water use has improved and the siltation levels reduced. Experience gained on this system has been successfully used in developing and managing other irrigation projects along the lower reaches of the Yellow River. The proposed procedure of solving the said optimal model has also embodied some benefits from reducing O & M costs in operation.  相似文献   

旱区覆膜滴灌棉田N 2 O排放对化肥减量有机替代的响应     

陶瑞  张前前  李锐  刘涛  褚贵新 《农业机械学报》2015,46(12):204-211

覆膜滴灌条件下,采用静态箱-气相色谱法研究了不同施肥策略:CK(不施肥)、CF(N 300 kg/hm~2;P2O590 kg/hm~2;K2O 60 kg/hm~2)、60%CF+OF(普通有机肥6 000 kg/hm~2)、60%CF+BF(生物有机肥6 000 kg/hm~2)对棉田土壤N_2O排放的影响,旨在明确滴灌棉田连续不同施肥策略下土壤N_2O的排放特征。结果表明,棉花生育期N_2O排放通量表现为施肥处理大于不施肥处理,滴灌施肥后第3/4天N_2O排放通量顺序为CF60%CF+OF60%CF+BFCK,而滴灌后第7/8天N_2O排放通量则表现为有机肥处理高于化肥处理,滴灌施肥结束后表现与之相同;生育期的N_2O排放总量以100%化肥处理(CF)最高,与其相比,60%CF+OF和60%CF+BF处理分别降低3.75%和8.37%,N_2O排放系数则分别降低1.39%和73.8%;相关及通径分析均表明,与土壤NH+4-N相比,NO-3-N与N_2O排放的关系更密切。  相似文献   

Soil nitrogen and denitrification potential as affected by land use and stand age following agricultural abandonment in a headwater catchment     

K. ZHANG  X. CHENG  H. DANG  C. YE  Q. ZHANG 《Soil Use and Management》2012,28(3):361-369

Changes in vegetation and soil properties because of agricultural abandonment may affect soil nitrogen (N) and associated processes. We investigated soil N (total N: TN, inorganic N: NH₄–N and NO₃–N) and denitrification potential in cropland, pine plantations and abandoned agricultural land along a secondary succession sequence (grassland→shrubland→secondary forest) in a headwater catchment in the Qinling Mountains, northwest China. The results show that the soil denitrification potential differed significantly among the five land‐use types with the highest potential in the secondary forest, followed by grassland, shrubland, cropland and plantations. The denitrification potential of the 20‐ to 40‐cm layer was significantly lower compared with the topsoil (0–20 cm) across all land‐use types. TN, soil organic matter (SOM) and NH₄–N increased significantly with stand age, whereas there was an opposite trend in soil pH. However, the denitrification potential did not relate to stand age in a linear manner. We conclude that changes in soil TN, SOM and pH during vegetation succession following agricultural abandonment are critical controls on the denitrification potential.  相似文献   

纳米CuI催化偶联反应合成二苯醚类农药中间体     

潘攀  蔡振亚  叶加久  杨春燕  徐小岚 《现代农业科技》2010,(9):25-26

二苯醚是一种重要的农药中间体,通过W/O微乳法,制备出形貌均一的球状纳米CuI,并应用于催化C-O交叉偶联反应中,在温和的反应条件下,合成了具有优良产率的二苯醚类化合物,该方法可在今后的农药合成中应用。  相似文献