利用酵母双杂交系统筛选与草莓镶脉病毒移动蛋白P1互作的寄主因子     

张享享  蒋西子  李帅  蒋磊  江彤 《植物病理学报》2019,49(1):56-63

 构建感染草莓镶脉病毒(SVBV)森林草莓的酵母cDNA文库,利用酵母双杂交系统,筛选出与SVBV P1蛋白互作的15种寄主因子。生物信息学分析发现,这15种寄主因子参与茉莉酸途径、泛素化、光合作用、抗病抗逆、蛋白修饰、蛋白运输和氧化还原等多种生物过程。另外,这些寄主因子还具有其他分子功能,包括氧化还原酶活性、蛋白二硫化物异构酶活性和金属离子结合活性等。本研究初步探讨了P1与寄主因子的互作机理,为揭示SVBV侵染森林草莓以及SVBV在寄主中扩展的分子机制提供理论依据。  相似文献   

氯虫苯甲酰胺和氟虫腈对黏虫细胞色素P450基因表达的影响     

刘俊杰  苏栩  李亚设  谢兰芬  张百重  陈锡岭 《植物保护学报》2019,46(3):563-573

为筛选出黏虫Mythimna separata参与杀虫剂解毒代谢的主效细胞色素P450基因,采用叶片浸渍法测定了用于处理黏虫3龄幼虫的亚致死浓度,通过构建转录组测序文库并结合数字基因表达(digital gene expression,DGE)对不同处理的黏虫进行测序,并运用实时荧光定量PCR(realtime quantitative polymerase chain reaction,RT-qPCR)技术验证12个P450基因的表达情况。结果表明,用于处理黏虫的氯虫苯甲酰胺和氟虫腈亚致死浓度LC_(10)分别为0.15、13.66 mg/L;对照样品、氟虫腈处理样品和氯虫苯甲酰胺处理样品分别获得59 521 504、64 838 148和41 722 990个原始序列数据,分别获得57 441 216、62 368 912和40 285 164个过滤后的序列数据;过滤后的序列长度分别为8.62、9.36和6.04 G;碱基错误率均为0.02%;Phred数值大于20、30的碱基占总碱基的百分比均高于90.59%;鸟嘌呤+胞嘧啶(guanine cytosine,GC)含量分别为47.16%、48.94%和47.55%,表明转录组测序质量较高;黏虫受氯虫苯甲酰胺胁迫后,29个P450基因表达量上调,27个P450基因表达量下调;黏虫受氟虫腈胁迫后,23个P450基因表达量上调,26个P450基因表达量下调;12个P450基因表达量的RT-qPCR技术检测结果与DGE测序文库显示的结果基本一致。  相似文献   

Characterization of sulfoxaflor resistance in the brown planthopper,Nilaparvata lugens (Stål)     

Xun Liao  Ruoheng Jin  Xiaolei Zhang  Ehsan Ali  Kaikai Mao  Pengfei Xu  Jianhong Li  Hu Wan 《Pest management science》2019,75(6):1646-1654

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Trp‐1999‐Ser mutation of acetyl‐CoA carboxylase and cytochrome P450s‐involved metabolism confer resistance to fenoxaprop‐P‐ethyl in Polypogon fugax     

Ning Zhao  Lu'an Ge  Yanyan Yan  Shuang Bai  Dandan Wang  Weitang Liu  Jinxin Wang 《Pest management science》2019,75(12):3175-3183

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All-trans retinoic acid attenuates blood-brain barrier injury induced by cerebral ischemia-reperfusion in rats through JNK/P38 MAPK signaling pathway     

LI Ming-hang  TIAN Xiao-cui  AN Rui-di  ZHANG Qian  YANG Mei  XIANG Fei  WANG Yu-chun  XU Lu  DONG Zhi 《园艺学报》2019,35(1):112-118

AIM: To investigate the effect of all-trans retinoic acid (ATRA) on blood-brain barrier after cerebral ischemia-reperfusion (CIR) injury in rats and its possible role mechanism.METHODS: Male SD rats were randomly divided into sham group, model (CIR) group and CIR+ATRA (10, 30 and 90 mg/kg) groups. The rat model of CIR injury was established by MCAO thread occlusion method. After ischemia for 1.5 h and reperfusion for 24 h, the neurological functional behavioral score, cerebral infarction volume, brain water content and Evans blue content were determined. The activity of matrix metalloprotein-9 (MMP-9) was measured by gelatin zymography. The protein levels of claudin-5, occludin, ZO-1, JNK, p-JNK, P38, p-P38 and MMP-9 in the brain tissues were determined by Western blot.RESULTS: Compared with CIR model group, ATRA at 30 mg/kg significantly improved neurological function, and decreased cerebral infarction volume, brain water content, Evans blue content and the degradation of tight junction proteins in ischemic area (P<0.01). The activity and protein expression of MMP-9 in ischemic brain tissue were decreased (P<0.01). The phosphorylation of JNK and P38 was inhibited and the protein levels of p-JNK and p-P38 were decreased (P<0.01).CONCLUSION: ATRA reduces the damage of brain tissue and the destruction of blood-brain barrier induced by CIR in rats. The protective effect may be related to inhibiting the activation of JNK/P38 MAPK signaling pathway and MMP-9.  相似文献   

Correlation between renal ischemia-reperfusion injury and nucleolar stress     

XIE Hong-sheng  CAO Yuan  LE Chang-hao  LI Jia-qi  LI Bo-wen  KUANG Xiao-dong  XIAO Jian-sheng 《园艺学报》2019,35(7):1243-1247

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miR-204 inhibits proliferation of Hodgkin lymphoma cells by targeting Sirt1/p53 signaling pathway     

ZHENG Xiao-qiang  RUI Hong-bing  ZHANG Guang-hui 《园艺学报》2019,35(9):1557-1564

AIM: To investigate the effect of microRNA-204 (miR-204) on the proliferation of Hodgkin lymphoma cells and the underlying mechanism. METHODS: The expression of miR-204 and Sirt1 mRNA in Hodgkin lymphoma tissues was detected by RT-qPCR. After transfection with miR-204 mimic, Sirt1 siRNA and miR-204 mimic+pcDNA3.1-Sirt1 into the L428 cells, the cell viability and BrdU incorporation were measured by CCK-8 assay and BrdU assay, respectively. The protein levels of Sirt1 and acetylated p53 (ac-p53) were determined by Western blot.The targeting relationship between miR-204 and Sirt1 was verified by double luciferase reporter assay. RESULTS: The low expression of miR-204 and the high mRNA expression of Sirt1 were found in the Hodgkin lymphoma tissues. Compared with control group, the cell viability, BrdU incorporation and the protein levels of Sirt1 and ac-p53 were significantly decreased after L428 cells were transfected with miR-204 mimic or Sirt1 siRNA (P<0.05). Compared with miR-204 mimic alone group, the cell viability, BrdU incorporation and the protein levels of Sirt1 and ac-p53 were increased after L428 cells were co-transfected with miR-204 mimic and pcDNA3.1-Sirt1 (P<0.05). The results of double luciferase reporter assay confiermed that Sirt1 was the target gene of miR-204. CONCLUSION: The inhibitory effect of miR-204 on the proliferation of L428 cells may be achieved by inhibiting the expression of Sirt1 and promoting the up-regulation of ac-p53.  相似文献   

温室3P3R机械臂系统动力学建模与分析   总被引：1，自引：0，他引：1  

焦有宙  丁攀  赵大旭 《农业机械学报》2012,43(5):179-183

针对温室等设施农业环境,设计了一种具有3P3R机械臂结构的机器人,为了分析机械臂的操作性能并实现精确运动控制,对机械臂进行了运动学和动力学分析;采用Kane方法和旋量理论分析方法建立了机器人的操作臂运动学和动力学模型,利用该模型,针对原理样机的具体结构,在Mathematica环境下研究了机械臂的操作性能,得到在一定作业任务规划下,末端执行器的位姿变化规律,以及按照该规划轨迹运动时各关节的驱动力;结果表明,结合了Kane方法和旋量理论的动力学模型具有准确、简单、有效等特点,能够满足机械臂的运动学、动力学分析的要求。  相似文献   

石灰性土壤无机磷的形态分布及其有效性   总被引：66，自引：1，他引：66  

沈仁芳  蒋柏藩 《土壤学报》1992,29(1):80-86

本文应用蒋柏藩和顾益初(1989)提出的石灰性土壤无机磷的分级方法,对我国北方主要的石灰性土类进行了无机磷形态分级的研究,并对其有效性作出了初步评价。供试的甘肃、陕西和河南的16种土壤的无机磷形态的分布情况为:Ca₂-P平均占无机磷总量的1.34%,Ca-P占9.91%,Al-P占4.27%,Fe-P占4.40%,O-P占10.9%,Ca₁₀-P占69.1%。生物试验的结果表明:Ca₂-P型的磷酸盐是最有效的,也是作物磷素营养的主要来源;Ca₈-P、Al-P和Fe-P可以作为缓效磷源;Ca₁₀-P和O-P只是一种潜在磷源。本研究为石灰性土壤无机磷的研究和磷肥的合理施用提供了理论依据。  相似文献   

水稻对红壤中N、P的吸收利用   总被引：1，自引：1，他引：1  

傅志坚  王德先 《核农学报》1992,6(4):214-218

试验结果表明,当施氮量一定的情况下,增施磷、钾肥,氮肥利用率和水稻的产量都有显著的增加。谷粒产量和氮肥利用率为29.78g/盆和44.37％,分别比对照(单施氮肥)提高22.75％和9.62％。合理施用氮、钾肥,磷肥的利用率也有显著增加(为29.10％),比单施磷肥利用率提高6.88％。  相似文献