黑曲霉植酸酶对肉仔鸡生长和磷利用率影响的研究     

丁奕 《水禽世界》2003,(9):17-18

选用180只1日龄AA肉鸡,随机分为1个对照组和1个试验组。研究在低有效磷日粮中添加黑曲霉AsN1980植酸酶干曲对肉仔鸡生长性能及磷利用的影响。研究结果表明,植酸酶对肉鸡生长和饲料中磷利用率有明显促进作用。料肉比减少0.233～0.256,磷的利用率提高7.63%～8.27%,均优于对照组(P<0.05)。  相似文献   

常用植物性饲料中的植酸酶活性及加热温度的影响   总被引：3，自引：0，他引：3  

韩延明  周安国  杨凤 《动物营养学报》1995,(3)

在pH5,0,37℃,反应15分钟的条件下测定了小麦、大麦、豆饼、玉米、菜籽饼和麦麸中的植酸酶活性(PU/g干物质),它们分别为1454、1278、204、183、334和2349。麦麸中植酸酶有较好的耐热性,在60℃或80℃的温度下加热1小时,其活性仅下降了7％,但在100℃的温度下加热1小时,植酸酶的活性却下降60％。  相似文献   

Phyzyme XP phytase improves growth performance and nutrient utilization in wheat-based diets fed to weaned pigs     

J.S. Sands  R.M. Kay 《Livestock Science》2007,109(1-3):264-267

The effect of phytase (Phyzyme XP) on performance and nutrient utilization in piglets fed wheat-based diets was evaluated using 24 male pigs averaging 7 kg. Four experimental diets containing either 0 (positive control, adequate nutrients), 0 (negative control (NC), reduced nutrients), 500 or 1000 U/kg of phytase were used for each of two dietary phases (day 0 to 14 and day 14 to 33). Feed and water were provided ad libitum. Animals were weighed at weekly intervals until day 28 and feed intake (DFI) recorded daily. From day 28 to 33 pigs were fed at 3 times maintenance energy intake and DFI recorded. Total amounts of faeces and urine were recorded for each individually housed pig. Digestibilities and balance of dry matter (DM), phosphorus (P), calcium (Ca), nitrogen (N) and gross energy (GE) were calculated for feed, faeces and urine. Compared to the NC, daily live weight gain was improved (P < 0.05) for pigs receiving the highest level of supplemental phytase in phase 2. Phytase addition at 1000 FTU/kg also increased faecal digestibilities of P and Ca (P < 0.05). Contrasts comparing NC and Phytase diets showed that Ca (P < 0.02) and P (P < 0.05) balance were improved.  相似文献   

Heat stability of endogenous and microbial phytase during feed pelleting     

B.A. Slominski  T. Davie  M.C. Nyachoti  O. Jones 《Livestock Science》2007,109(1-3):244-246

Heat stability of commercial preparations of phytase has been of concern for quite some time, and efforts have been made to develop new preparations of this enzyme. A study was conducted to determine the stability of commercially available phytase product (granulate; Phytase A) and the new experimental product (powder; Phytase B). In the in vitro study, incubation of 100 mg of each of Phytase A and B with 200 μl of buffer for 2 min (30 s at desired temperature) resulted in 27.6 and 10.4% loss of activity at 60 °C and 80.6 and 53.9% at 70 °C, respectively. Both enzyme products were further subjected to steam pelleting in feed mills located in Manitoba and British Columbia. In the Manitoba study, the temperature of pellets as recorded at the discharge averaged 67 °C and was similar to that determined in British Columbia (70 °C). Under such temperatures, which may have been lower than the actual temperatures within the pellet mill, the loss of endogenous phytase activity averaged 58.5% (from 451 to 187 U kg^− 1) and 42.5% (from 287 to 165 U kg^− 1) in the two mills, respectively. Following correction for endogenous phytase activity, Phytase A and B recovery averaged 36.4 and 49.9%, respectively, at the Manitoba site and 44.1 and 49.4% at the British Columbia site. It appears evident from this study that the heat stability of enzyme (protein) per se rather than the granulation technology is a primary factor determining the stability of microbial phytase during steam pelleting.  相似文献   

生长猪植酸酶的磷当量研究   总被引：6，自引：0，他引：6  

邓近平  姜洁凌  贺建华  范志勇  袁旭鹏 《动物营养学报》2007,19(2):166-171

本文旨在研究饲粮中添加植酸酶对植酸磷利用的影响,确定生长猪的植酸酶磷当量。本试验采用单因素试验设计,以NaH2PO4.2H2O提供外源无机磷,同时添加植酸酶,设计1个低磷基础日粮、4个加磷日粮和3个加酶日粮,分别提供0.4、0.8、1.2、1.6g/kg的有效磷或500、750、1000FTU/kg植酸酶。选择96头体重22kg左右的杜长大三元杂交猪,随机等分到8个处理中。通过回归分析,建立响应指标(猪趾骨折断强度和趾骨灰分磷含量)与外源磷添加量之间的线性关系,进而确定植酸酶的磷当量值。结果表明:(1)随外源磷和植酸酶水平的提高,试验猪生产性能明显改善(P<0.05),趾骨灰分磷含量和血磷水平都极显著地升高(P<0.01);(2)无机磷添加水平与响应指标之间存在较强线性关系:y=10.79x 27.23(x-添加磷(g/kg),y-趾骨强度(kg),R2=0.92);y=1.44x 6.86(x-添加磷(g/kg),y-灰分磷(%),R2=0.99);(3)植酸酶的磷当量值与日粮中植酸酶和无机磷水平密切相关,同时也受相应指标种类的影响。本试验条件下,500、750、1000FTU/kg植酸酶分别可替代1kg玉米-豆粕日粮中0.09g、0.44g和0.72g(折断强度)或0.23g、0.58g、1.06g(灰分磷)无机磷。  相似文献   

金针菇产植酸酶菌株的筛选及植酸酶基因区域的克隆   总被引：1，自引：0，他引：1  

刘世强  郭丽琼  林俊芳  郭安平 《食用菌学报》2005,12(3):1-6

从金针菇（Flammulina velutipes）7个菌株中筛选出1株高产植酸酶菌株.根据GenBank中植酸酶基因的保守区设计并合成一对特异性引物,以金针菇菌丝的总DNA为模板,通过PCR扩增,获得了一条长约790bp的片段.DNA序列测定结果表明,该片段长度为788bp,采用Blast软件进行序列比对发现,该片段与平田头菇（Agrocybe pediades）的植酸酶基因phy（GenBankAccession：CAC48160）编码的氨基酸具有64%的序列同源性.该片段含有2个内含子,含有植酸酶基因的活性位点高度保守序列（Active-site sequence）-RHGXRXPT.  相似文献   

25-OH-D3、维生素D3与植酸酶组合对肉仔鸡养分利用的影响     

韩明霞  魏时来  杨德智  李跃胜  都怡 《国外畜牧学(猪与禽)》2008,28(4)

本试验研究了25-OH-D3、维生素D3、植酸酶不同的水平组合对肉仔鸡养分利用的影响。试验分6个处理组,每个组的25-OH-D3、维生素D3和植酸酶的添加水平分别为:第1组(0μg/kg、2760IU/kg和0FTU/kg)、第2组(69μg/kg、0IU/kg和0FTU/kg)、第3组(69μg/kg、2760IU/kg和0FTU/kg)、第4组(0μg/kg、2760IU/kg和750FTU/kg)、第5组(69μg/kg、0IU/kg和750FTU/kg)、第6组(69μg/kg、2760IU/kg和750FTU/kg)。在生长试验的后期进行代谢试验,每组4只鸡(艾维茵肉仔鸡,♂),即4个重复。结果表明:肉仔鸡日粮中添加植酸酶能提高干物质、总能、粗蛋白、钙、总磷的利用率,25-OH-D3和维生素D3的添加效果相似,且超量添加对营养物质的利用并无好处,第4和5组是比较理想的组合。  相似文献   

农杆菌介导植酸酶基因转化大豆的研究   总被引：1，自引：0，他引：1  

刘琦  夏善勇  张军  段忠卫  刘鑫磊  李希臣 《大豆科学》2011,30(3)

以大豆胚尖为受体,利用农杆菌介导法将来源于泡盛曲霉的植酸酶基因phy转入黑农37和吉林小粒豆中。此法取材方便,操作简单,产生嵌合体的可能性低。在筛选培养基中经草胺膦(1.0 mg.L-1)筛选,获得抗性植株。对获得的草胺磷抗性植株采用除草剂(10%Basta)筛查表型鉴定与目的基因、筛选标记基因PCR分子检测相结合的方法,检测结果直观、可信度高。最终获得5株阳性植株,转化率为0.5%。  相似文献   

低磷饲粮添加植酸酶对肉仔鸡生长性能及消化率的影响   总被引：1，自引：0，他引：1  

尹红轩  李伟  张光辉  王晓可 《安徽农业科学》2009,37(6):2506-2507

以160只1日龄健康艾维因肉鸡为试材,研究在低磷饲粮中添加植酸酶（60 IU/kg）对其生长性能及养分消化率的影响。结果表明,添加植酸酶对肉仔鸡的日增重、耗料增重比、粗蛋白质和粗脂肪的消化率无显著影响（P〉0.05）,但能显著提高饲粮中钙和磷的消化率（P〈0.05）。  相似文献   

宇佐美曲霉植酸酶phy A基因的克隆     

王郡美  宋维平  张莉 《华北农学报》2006,21(3):5-8

植酸酶是催化植酸及其盐类物质水解成肌醇和磷酸的一类酶的总称,用其作为动物饲料添加剂既可以达到提高磷利用率的目的,又可以降低环境中的磷污染。真正具有开发价值的是利用微生物生产植酸酶。直接以宇佐美曲霉菌株2418基因组DNA为模板,对植酸酶基因phyA的成熟肽(分子长度为1 347 bp)进行了PCR扩增,再将PCR产物克隆入pBS-T载体,经DNA测序鉴定,与已发表的植酸酶基因同源性达到99%以上,这为进一步获得大量、高活性植酸酶以及研究和开发新型微生态制剂打下了基础。  相似文献