Gene Discovery and Characterization from Non-sequenced Medicinal Plants     

D. Inzé 《分子植物育种》2007,5(2):169-169

Plant-derived secondary metabolites still constitute important resources for currently prescribed drugs and for the discovery of active principles which are effective in new indication areas. Promising compounds are often exclusively found in wild medicinal plants and the classical plant-based pharmaceutical production approach has been hampered by low yields, lack of reproducibility and the high complexity of plant extracts （Rischer and Oksman-Caldentey, 2005）. Influencing the accumulation of target compounds by breeding or genetic engineering is greatly impeded because in most cases the general biosynthetic routes are incompletely understood.  相似文献   

昆虫取食和剪叶刺激对油松针叶内部分防御物质的诱导效应     

石媛媛  冯金周  于连海  高宝嘉 《河北农业大学学报》2017,40(1)

为了研究自然条件下昆虫取食及剪叶刺激对油松诱导抗性的影响,本试验应用香草醛—盐酸法,亚硝酸钠—硝酸铝比色法以及紫外分光光度法,分析了剪叶和不同程度油松毛虫取食处理后,混交林和纯林中油松针叶内部分次生代谢物和蛋白酶抑制剂活性的动态变化。结果表明:剪叶及不同程度油松毛虫取食能够诱导缩合单宁、黄酮含量和胰蛋白酶抑制剂、胰凝乳蛋白酶抑制剂活性增加。与对照相比,胰凝乳蛋白酶抑制剂在混交林中的活性比纯林更为明显。说明剪叶及昆虫取食可诱导增加油松针叶内的缩合单宁和黄酮含量、提高胰蛋白酶抑制剂和胰凝乳蛋白酶抑制剂的活性,进而增强油松的抗虫性,林分类型可在一定程度上影响油松诱导防御物质的变化。  相似文献   

宁夏农民中专学历教育探析   总被引：1，自引：1，他引：0  

田野  王丽慧  孙庆礼  赵红梅 《宁夏农林科技》2012,53(5):133-134

结合工作实际,从宁夏农民接受教育的现状入手,分析了农民学历教育存在的一些问题,探索了解决问题的办法。  相似文献   

景泰川灌区次生盐渍化及其防治     

李朝刚 《干旱区研究》1989,6(2):54-56

景泰川灌区位于甘肃省北部,景泰县内。1974年景泰电力提水工程建成,最大提水高度406.2米,设计流量10.5米/秒,有效灌溉面积30万余亩。本区为干旱区大陆性气候,年平均降水量186毫米,蒸发量3600毫米,且风沙多。过去由于没有灌溉条件,大面积土地长期荒芜。景电工程的建成,彻底改变了本区的自然面貌和生产条件,短短的十四年间,这里建成了荒漠中的大片绿洲。但是,随着土壤灌溉,灌区的次生盐渍化已经露头。根据第二次土壤普查统计,己经盐化面积30482亩,占总耕地面积的10.16%。因此,充分发挥提黄河水灌溉的效益,并防治土  相似文献   

荧光93菌体和代谢物对全蚀病菌和小麦生长影响的初步研究     

张中鸽  彭于发  黄大昉  陈彩层  张杰 《中国生物防治》1992,(3)

对荧光假单胞菌工程菌剂“荧光93”菌体和代谢物的作用特性进行了比较。结果表明:该菌剂能保护小麦根系免遭全蚀病危害,对植株生长有促进作用。菌体的抑菌能力强于代谢物,浸种处理防效分别为44.8％和27.8％;代谢物具有更强的刺激植株生长作用,在无病条件下,代谢物灌根处理鲜重增加34.5％,菌体处理鲜重增加7.5％。培养基成份对代谢物的作用有一定影响:以马铃薯葡萄糖培养产生的代谢物,抑菌效果好;营养肉汁酵母粉培养产生的代谢物,促生作用好。据此推断,荧光93菌剂代谢产物中含有抑制全蚀病菌和刺激植株生长的物质。  相似文献   

两种拟青霉菌提取物对菜蚜的控制作用   总被引：1，自引：0，他引：1       下载免费PDF全文

徐伟松  ;钟国华;李畅方;罗建军;胡美英 《植物保护》2007,33(6):35-38

浸叶法毒力测定结果表明,淡紫拟青霉(Paecilomyces lilacinus)菌株MCWA18和拟青霉(Paecilomycessp.)菌株X1甲醇萃取物对萝卜蚜无翅成蚜48 h后LC50分别为0.3435 mg/mL和0.8309 mg/mL。网室盆栽试验,菌株MCWA18和X1甲醇萃取物2.5 mg/mL处理7 d后对菜蚜的防治效果分别为92.24%和87.06%,田间小区试验处理后7 d,防治效果分别为81.82%和81.70%,相当于1.3%鱼藤氰乳油500倍的防效。  相似文献   

Genetic and physiological evidence for the production of N-acyl homoserine lactones by Pseudomonas syringae pv. syringae and other fluorescent plant pathogenic Pseudomonas species     

C. Korsi Dumenyo  Asita Mukherjee  Wesley Chun  Arun K. Chatterjee 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(6):569-582

N-acyl homoserine lactones (AHLs) function as cell density (quorum) sensing signals and regulate diverse metabolic processes in several gram negative bacteria. We report that strains of Pseudomonas syringae pvs. syringae (Pss), tabaci and tomato as well as P. corrugata and P. savastanoi produce difussible AHLs that activate the lux operons of Vibrio fischeri or the tra::lacZ fusion of Agrobacterium tumefaciens. In Pss strain B3A, AHL production occurs in cell density dependent manner. Nucleotide sequence and genetic complementation data revealed the presence of ahlI_Pss, a luxI homolog within the Ahl⁺ DNA of Pss strain B3A. The DNA expresses in AHL-deficient strains of P. fluorescens and E. carotovora subsp. carotovora (Ecc), and restores extracellular enzyme production and pathogenicity in the Ecc strain. The derivatives of Pss strains B3A and 301D carrying chromosomal ahlI::lacZ do not produce AHL, but like their wild type parents, produce extracellular protease and the phytotoxin syringomycin as well as elicit the hypersensitive reaction in tobacco leaves. While these strains also produce a basal level of -galactosidase activity, the expression of ahlI::lacZ is substantially stimulated in the presence of multiple copies of the DNA or by the addition of cell-free spent cultures containing AHL. The activation of -galactosidase production occurs with spent cultures of some, but not all Pseudomonas strains which produce AHL as indicated by the Lux and tra::lacZ assays. Pss strains deficient in the global regulatory genes, gacA or lemA, produce very low levels of AHL. Since inactivation of ahlI_Pss eliminates AHL production and since Ahl⁺ Pseudomonas strains carry the homolog of ahlI_Pss, we conclude that ahlI_Pss specifies a key step in AHL biosynthesis and it has been conserved in many plant pathogenic pseudomonads.  相似文献   

Comparative Disposition Kinetics of Albendazole in Sheep Following Oral and Intraruminal Administration     

Swarnkar CP  Sanyal PK  Singh D  Khan FA  Bhagwan PS 《Veterinary research communications》1998,22(8):545-551

The pharmacokinetics of albendazole was studied in sheep following single oral and intraruminal administration at nematocidal dose rates. The disposition curves of its metabolites indicated increased uptake of the drug in sheep following intraruminal as compared to oral dosing (p<0.05). The increased bioavailability of benzimidazole anthelmintics given by the intraruminal route could be exploited for optimizing the use of anthelmintic for sustained parasite control in small ruminants.  相似文献   

添加苯二氮化合物F89对皖西白鹅生长和代谢性能的影响     

葛盛芳  葛继志  韩正康  陈杰 《中国家禽》1998,(10)

利用生理调节剂Ｆ８９提高皖西白鹅的摄食量,加速动物生长。日粮中添加２ｍｇ／ｋｇＦ８９,连续饲喂８～３５日龄皖西白鹅,日摄食量较对照组增加６．０２％,增重提高９．４０％,耗料比下降２．８９％。对育肥期鹅的增重也有明显影响。血液代谢物分析表明：饲喂Ｆ８９后,皖西白鹅血清中葡萄糖和尿酸浓度下降,β－脂蛋白浓度上升。提示Ｆ８９不仅能促进鹅的摄食量,而且可以影响鹅的代谢机能,促进生长  相似文献   

中草药饲料添加剂对河西肉牛血液生化指标影响的研究     

周学辉  杨世柱  李伟  郭兆斌 《中国草食动物》2012,(6):23-25

为了提升河西肉牛产品特别是血液制品的质量及产业效益,利用饲料中添加中草药添加剂的方法对河西肉牛架子牛进行了育肥、屠宰试验。采取低剂量中草药组、高剂量中草药组和对照组肉牛血样,用专门仪器和方法测定了血液中5种血清矿物质离子、7种血清代谢产物及5种血清酶等生化指标的含量。结果表明:高剂量中草药组肉牛血清中的Na、Mg离子及乳酸脱氢酶含量均极显著高于低剂量组和对照组(P<0.01),Cl离子含量极显著高于对照组(P<0.01),其葡萄糖、肌酐、甘油三酯含量均显著高于对照组(P<0.05),谷丙转氨酶显著低于其他两组肉牛(P<0.05),而谷草转氨酶(AST)却显著高于其他两组肉牛(P<0.05);低剂量组和高剂量组肉牛血清中的高密度脂蛋白极显著高于对照组(P<0.01),碱性磷酸酶极显著低于对照组肉牛(P<0.01),而谷丙/谷酸则显著高于对照组肉牛(P<0.05)。结论:试验配制的中草药饲料添加剂对肉牛血液生化指标有良好的影响,对高剂量组肉牛的影响更为明显;饲喂中草药饲料添加剂的肉牛生长发育、营养状况均显著好于对照组,但高剂量组更优;利用高剂量中草药饲料添加剂饲喂的肉牛,其血液在制造保健用品方面有广阔开发前景。  相似文献