沙芥幼苗叶片小热激蛋白基因的克隆与表达     

于晓婧  王萍  武兆昕  杨永升  许珂  尚鹏 《湖南农业大学学报(自然科学版)》2021,47(6)

在对转录组和蛋白组数据联合分析的基础上,通过RT–PCR方法克隆得到沙芥幼苗叶片2个小分子热激蛋白基因PcHSP26.5和PcHSP17.8,运用生物信息学软件分析它们的核苷酸和编码蛋白,通过Real–time PCR分析其在高温、低温、NaCl、ABA和干旱复水胁迫下的表达模式。PcHSP26.5和PcHSP17.8开放阅读框分别为699 bp和462 bp,分别编码282和153个氨基酸,其中PcHSP26.5包含1个内含子。PcHSP26.5、PcHSP17.8的相对分子质量分别为26 480、17 490,理论等电点分别为6.36、5.99;均无跨膜结构与信号肽位点,属于亲水蛋白。亚细胞定位预测和进化树分析表明,PcHSP26.5主要定位于线粒体中,属于MⅡ亚族;PcHSP17.8主要定位于细胞质中,属于CⅠ亚族。实时荧光定量PCR结果表明：高温胁迫下,沙芥PcHSP26.5和PcHSP17.8基因的相对表达量均在1 h达到顶峰,之后下降;低温胁迫下,沙芥PcHSP26.5基因的相对表达量在0.5 h达到顶峰,之后下降,沙芥PcHSP17.8基因的相对表达量在0.5~12 h内低于对照,24 h时出现上升趋势;NaCl胁迫下,沙芥PcHSP26.5和PcHSP17.8基因的相对表达量分别在 1 h和3 h达到顶峰,之后下降;ABA胁迫下,沙芥PcHSP26.5和PcHSP17.8基因的相对表达量均在2 h达到顶峰,之后下降;干旱复水胁迫下,沙芥PcHSP26.5和PcHSP17.8基因的相对表达量均在干旱9 d达到顶峰,复水后下降。  相似文献   

外部冲击对蔬菜价格波动的影响——基于面板向量自回归模型（PVAR）的实证分析   总被引：1，自引：0，他引：1  

罗超平  李伟毅  翟琼 《中国蔬菜》2013,1(10):16-22

构建面板向量自回归模型（PVAR）的基础上，实证分析了外部冲击对蔬菜价格波动的影响，
结果发现：除GDP 增长率对蔬菜价格波动具有明显的冲击影响外，其他变量对蔬菜价格方差贡献都非常小，
在1.5%～3.3% 之间。同时，从变量冲击反应观察，当给蔬菜补贴LBT 一标准差冲击，蔬菜价格LJG 表现
为负响应并在第3 期达到最大；当给蔬菜成灾面积LCZ 一标准差冲击，蔬菜价格LJG 最初表现为负响应
并在第3 期转为正响应；当给GDP 增长率一标准差冲击，蔬菜价格LJG 表现为明显的正响应；当给城镇
居民收入LCZSR 一标准差冲击，蔬菜价格LJG 表现为负响应。由此可见，蔬菜价格对外部冲击反应的效
果并不理想，对部分外部冲击的反应存在明显的不合理的滞后性。健全蔬菜产业市场机制、提高蔬菜流通
效率、保障蔬菜供需平衡等是抑制蔬菜价格剧烈波动的重要措施。  相似文献   

Identification of cDNA for the HSPA2, HSPA5 and HSPA8 orthologs of the heat shock protein 70 family from guinea fowl (Numida meleagris)     

Shigehisa IWAMOTO  Yohei KOIKE  Kazuyoshi HOSOMICHI  Hiromi HARA  Yutaka YOSHIDA  Hiroshi OGAWA  Kei HANZAWA 《Animal Science Journal》2005,76(5):519-524

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Amelioration of Drought-Induced Transplanting Shock in Rice by an Abscisic Acid Analog in Combination with the Growth Retardant Tetcyclacis     

A. A. Flores-Nimedez  K. Dörffling  B. S. Vergara 《Journal of Agronomy and Crop Science》1995,174(3):145-150

The protective effect of a synthetic terpenoid analog of abscisic acid (ABA), coded LAB 173711, on transplant shock in rice was investigated under field conditions. Rice seedlings (Fujisaka 5 and IR 20) were taken from a seed bed and their roots were immersed in aqueous solutions of the growth regulators for 24 h. Afterwards they were drought-stressed for 3 or 6 h by exposing them to direct sunlight before they were transplanted to the field. A pretreatment with either LAB 173711 alone or in combination with the growth retardant tetcyclacis reduced the loss of water during the exposure to sunlight, increased the percentage of survival of the seedlings in the field, and increased the percentage of grain filling of the plants which had survived. The combined application of growth regulators was most effective and may be useful for minimizing transplant shock in rice and other crops.  相似文献   

谷氨酰胺对免疫细胞的影响研究进展     

牟彬  周小秋  林燕 《动物营养学报》2007,19(Z1)

谷氨酰胺作为一种条件性必需氨基酸,在动物处于特殊生长阶段如幼龄期或在应激期间都显示出明显的免疫促进作用。动物免疫力的高低直接取决于各种免疫细胞如淋巴细胞、中性粒细胞、巨噬细胞的生长情况和免疫应答能力,谷氨酰胺的免疫促进作用与影响免疫细胞生长和免疫应答能力的发挥密切相关。本文就谷氨酰胺与免疫细胞增殖、分化、凋亡、分泌和热休克反应的研究进展做一综述。  相似文献   

Factors Associated with Survival in Septicemic Foals: 65 Cases (1988–1995)     

J'mai M. Gayle  Noah D. Cohen  M. Keith Chaffin 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1998,12(3):140-146

In order to identify variables obtained at admission that could be used to predict survival in septicemic foals, medical records of 65 foals diagnosed with septicemia were reviewed. Initially, variables were analyzed independently (univariate analysis) for association with survival. Of the physical examination and historical data examined using univariate analysis, the ability to stand at admission, respiratory rate ≥ 60 breaths per minute (bpm), and normal-appearing mucous membranes were significantly associated with survival. Foals with history of induced parturition were significantly less likely to survive. The following hematologic and serum biochemical variables determined at admission were significantly associated with survival: white blood cell count a 6,000 cells/μL, neutrophil count <4,000 cells/μL, serum albumin concentration <2.2 g/dL, serum glucose concentration <120 mg/ dL, blood pH ≥ 7.35, and positive base excess. The administration of plasma at admission was significantly associated with survival. Stepwise multiple logistic regression analysis was performed to evaluate the association between survival and variables identified as significantly associated with survival in bivariate analysis. The final multivariate model selected included the variables standing, duration of clinical signs (24-hour intervals) prior to admission, respiratory rate a 60 bpm, neutropenia (> 4,000 cells/μL), and neonatal age category. The probability of survival was significantly increased for foals that were standing, had a respiratory rate a 60 bpm, and that had a neutrophil count <4,000 cells/μL at admission. Probability of survival was significantly decreased for foals that had a longer duration of clinical signs prior to admission. For each 24 hours of duration, the estimated risk of death was increased by 5.8-fold.  相似文献   

A Simple Apparatus for Frequent Measurement of Gastrointestinal Intraluminal PCO₂     

Piper L. Wall DVM  PhD  †  Akella Chendrasekhar MD  †Gregory A. Timberlake MD  † 《Journal of Veterinary Emergency and Critical Care》1998,8(2):109-115

The accuracy and precision of an end-tidal infrared CO₂ monitor and customized capnography tubing system designed to measure gastrointestinal intraluminal CO₂ partial pressure (P_iCO₂) were tested in vitro. Samples were taken from the customized capnography tubing placed in either 5% or 10% CO₂ gas at discrete intervals ranging from one minute to twelve minutes. For a given time interval, the tubing PCO₂ measurement was a constant fraction of the actual PCO₂ (all standard errors < 0.02). For increasing time intervals, the ratio of the tubing PCO₂ to actual PCO₂ increased logarithmically. In the 5% and 10% CO₂, the regression coefficients were 0.89 and 0.85 for 8 French tubing and 0.99 and 0.91 for 6 French tubing. Beacuase of its accuracy and precision, this system may provide automated gastrointestinal CO₂ partial pressure monitoring at short intervals (e.g. 5 minutes), facilitating testing of the role of gastrointestinal P_iCO₂ information in treatment algorithms. (Vet Emerg & Crit Care, 1998; 8: 109–116)  相似文献   

热激处理对桃,李离体花枝2n花粉产生的影响   总被引：14，自引：1，他引：13  

张新忠  刘国俭 《园艺学报》1998,25(3):292-293

对桃和李通过休眠的花枝在２０℃条件下进行水培,减数分裂前期Ⅰ进行３２℃、１２ｈ热激处理,小孢子母细胞减数分裂四分体时期二分体发生频率为５．８２％～１６．０６％,２ｎ花粉比例为１３．３３％～２０．３７％,处理后花粉离体萌发率较低;３０℃恒温培养导致花蕾败育。二分体频率重复间、花朵间和品种间差异显著。  相似文献   

Induction of interspecific allo-tetraploids of Megalobrama amblycephala♀×Megalobrama terminalis♂ by heat shock     

Shuming Zou  Sifa Li  Wanqi Cai  Huaiyu Yang  & Xiayun Jiang 《Aquaculture Research》2008,39(12):1322-1327

Interspecific allo-tetraploidy was induced by a combination of hybridization of Megalobrama amblycephala ♀× Megalobrama terminalis ♂ and thermal shock. A thermal shock at 40 °C for 2 min applied to eggs 33 or 26 min (τ₀: 1.40) after fertilization resulted in a significantly ( P <0.01) higher yield of allo-tetraploids than hybrid crosses without heat shock. The yield of allo-tetraploids in embryos from mid-gastrula to hatching stages was 15.7–24.4% in different thermal shock treatment groups. The allo-tetraploidy rate in 330-day-old juveniles was 9.3% and 6.7% in different year treatment groups. After 2 years of rearing, allo-tetraploidy males attained sexual maturity with milk-like milt at the age of 2+, while allo-tetraploidy females at the age of 2+, and even at 3+, did not show any signs of sexual maturation exhibiting obviously delayed maturity. Only a few allo-tetraploidy females (three individuals) showed maturation characters at the age of 4+ and these females were induced to spawn.  相似文献   

The role of tumor necrosis factor-α (TNF-α) and the sphingosine pathway in sepsis-induced myocardial dysfunction     

Merilee F. Costello  DVM  DACVECC    Cynthia M. Otto  DVM  PhD  DACVECC    Leona J. Rubin  PhD 《Journal of Veterinary Emergency and Critical Care》2003,13(1):25-34

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