Infection with foot-and-mouth disease virus (FMDV) induces a natural killer (NK) cell response in cattle that is lacking following vaccination     

Jared R. Patch  Pervaiz A. Dar  Ryan Waters  Felix N. Toka  Jose Barrera  Christopher Schutta  Ganesh Kondabattula  William T. Golde 《Comparative immunology, microbiology and infectious diseases》2014

Natural killer (NK) cells play a role in innate antiviral immunity by directly lysing virus-infected cells and producing antiviral cytokines such as interferon gamma (IFN-γ). We developed a system for characterizing the bovine NK response to foot-and-mouth disease virus (FMDV), which causes a disease of cloven-hoofed animals and remains a threat to livestock industries throughout the world. IL-2 stimulation of PBMC resulted in poor killing of human K562 cells, which are often used as NK target cells, while lysis of the bovine BL3.1 cell line was readily detected. Depletion of NKp46-expressing cells revealed that 80% of the killing induced by IL-2 could be attributed to NKp46⁺ cells. In order to characterize the response of NK cells against FMDV in vivo, we infected groups of cattle with three different strains of the virus (A24 Cruzeiro, O1 Manisa, O Hong Kong) and evaluated the cytolytic ability of NK cells through the course of infection. We consistently observed a transient increase in cytolysis, although there was variation in magnitude and kinetics. This increase in cytolysis remained when CD3⁺ cells were removed from the preparation of lymphocytes, indicating that cytolysis was independent of MHC-T cell receptor interaction or γδ T cell activation. In contrast, animals monitored following vaccination against FMDV did not exhibit any increase in NK killing. These data suggest that NK cells play a role in the host immune response of cattle against FMDV, and contrast with the suppression of NK activity previously observed in swine infected with FMDV.  相似文献   

A systematic review of the efficacy of prophylactic control measures for naturally-occurring canine leishmaniosis,part I: Vaccinations     

C.E. Wylie  M. Carbonell-Antoñanzas  E. Aiassa  S. Dhollander  F.J. Zagmutt  D.C. Brodbelt  L. Solano-Gallego 《Preventive veterinary medicine》2014

Canine leishmaniosis (CanL) is an important zoonotic disease; however, the efficacy of available vaccines for the prevention of naturally-occurring Leishmania infantum (L. infantum) infection in dogs remains unclear.  相似文献   

Immune response and disease resistance in Atlantic salmon (Salmo salar L.) fed three levels of dietary vitamin E and the effect of vaccination on the liver status of antioxidant vitamins   总被引：3，自引：0，他引：3  

Bjarte Lygren  Brit Hjeltnes  Rune Waagbø 《Aquaculture International》2002,9(5):401-411

Atlantic salmon were fed 40, 300 or 1100 mgall-rac--tocopheryl acetate kg feed^–1 for 12 weeks.After 6 weeks half of the fish in each group were marked and vaccinated (i.p.injection) against furunculosis and vibriosis. Liver -tocopherol levelsreflected the dietary input after 6 and 12 weeks of feeding. Noimmunomodulatoryeffects of dietary vitamin E on baseline levels of the immune parameters beforevaccination were detected in this study, as evaluated by antibody dependent andspontaneous plasma complement activities. In general, vaccination increasedplasma complement activities and the number of antigen specific antibodyproducing cells as compared with unvaccinated control fish, but with nodifferences with respect to the vitamin E regimes. Also the ability ofunvaccinated fish to withstand experimental furunculosis was unaffected bydietary vitamin E. The concentrations of -tocopherol and ascorbic acidinthe liver were, however, negatively affected 6 weeks post vaccination comparedto respective unvaccinated fish.  相似文献   

Development of vaccination strategies for the management of rabies in African wild dogs     

F. Vial  G. Rasmussen 《Biological conservation》2006,131(2):180-192

The standard objective of a vaccination strategy is to reduce the reproductive ratio, R₀, defined as the number of secondary host infections arising directly from introduction of a single infected individual into an otherwise fully susceptible population, to below 1. This ensures that only very small outbreaks are likely to arise. However, this objective usually requires a high level of vaccination coverage that is often expensive and logistically difficult to achieve. For the purposes of conserving rare species that are threatened by outbreaks of infectious disease, population persistence may be assured by a vaccination strategy designed to suppress only the largest outbreaks of disease that reduce the population to below a minimum viable population size. Such strategies targeting only a viable minimal ‘core’ of the population are likely to be logistically less demanding. Here we explore how these core vaccination strategies might be designed for African wild dogs (Lycaon pictus), an endangered canid species whose remaining populations are threatened by rabies outbreaks. We develop and analyse a population viability model with an explicit epidemiological model embedded within it. The model predicts that core vaccination campaigns, using vaccines that provide two years of immunity, and targets 30-40% of individuals within a population every 1-2 years would be successful in ensuring persistence of small populations, and required coverage levels can be even lower in larger populations. This strategy appears robust to synchronized introduction of rabies into packs, possible Allee effects, and the use of vaccines providing only one year of immunity.  相似文献   

Social-ethical issues concerning the control strategy of animal diseases in the European Union: A survey   总被引：1，自引：0，他引：1  

Nina E. Cohen  Marcel A.P.M. van  Asseldonk  Elsbeth N. Stassen 《Agriculture and Human Values》2007,24(4):499-510

In 2004 a survey was conducted in the member states of the European Union designed to gain greater insight into the views on control strategies for foot and mouth disease, classical swine fever, and avian influenza with respect to the epidemiological, economic and social-ethical consequences of each of these animal diseases. This article presents the results of the social-ethical survey. A selection of stakeholders from each member state was asked to prioritize issues for the prevention and control of these diseases. A majority of stakeholders chose preventive measures as the preferred issue. An analysis was done to determine whether there were differences in views expressed by stakeholders from member states with a history of recent epidemics and ones without such a history, and whether there were regional differences. There were no differences between member states with or without a history of recent epidemics. There were indeed regional differences between the priority orders from Northern and Southern Europe on the one hand, and from Eastern Europe on the other. Nina E. Cohen is a biologist and is a researcher at the Wageningen University. She is specialized in societal and ethical issues in human–animal relationships. Her current research is focused on the social-ethical issues concerning the prevention and control of foot and mouth disease, classical swine fever and avian influenza. Marcel A.P.M. van Asseldonk has studied animal science. Currently he works at the Institute for Risk Management in Agriculture (IRMA) of the Wageningen University. He is specialized in the design and pricing of insurance policies and animal health funds for the main livestock epidemics. Elsbeth N. Stassen is a veterinarian and professor of Animals and Society at the Wageningen University. Elsbeth Stassen is specialized in animal health, animal welfare and human–animal relationships. She was a member of a governmental welfare committee during the avian influenza epidemic in the Netherlands in 2003.  相似文献   

Vaccination strategies to conserve the endangered African wild dog (Lycaon pictus)     

K.C. Prager  R. Woodroffe  A. Cameron  D.T. Haydon 《Biological conservation》2011,(7):1940-1948

Pathogens such as rabies virus and canine distemper virus present a significant risk to the long-term survival of some endangered African wild dog (Lycaon pictus) populations. Vaccination of wild dogs is one approach that might reduce population extinction risks; however clear guidelines for how best to do this are lacking. Hence, we developed a mathematical model of wild dog population dynamics that integrates a pathogen transmission model and a vaccination process. Simulations indicated that the most effective vaccination strategy includes all age classes (when pups are 2-months old), prioritizes the breeding pair, and vaccinates at least 4 animals per pack included in the vaccination effort. In addition, populations for which an Allee effect, high rates of pathogen introduction, or low rates of recovery and immunity were simulated, required greater vaccination coverage (dogs/pack), to protect an equivalent number of packs compared to populations for which no Allee effect, low rates of pathogen introduction, or high rates of recovery and immunity were simulated. For populations in which some packs (high-risk) have a greater risk of pathogen exposure than others (low-risk), vaccinating both high- and low-risk packs, or only low-risk packs, is more effective than vaccinating only high-risk packs when pathogen introduction rates are high. Finally, model results suggest that vaccination of wild dog populations against pathogens, such as canine distemper virus, that do not cause 100% mortality and against which some wild dogs develop acquired immunity, may be unnecessary.  相似文献   

Predictions for the timing and use of culling or vaccination during a foot-and-mouth disease epidemic     

Hutber AM  Kitching RP  Pilipcinec E 《Research in veterinary science》2006,81(1):31-36

First-fortnight incidence (FFI) is a modelling parameter that can be used to predict both the prevalence and duration of a foot-and-mouth disease (FMD) epidemic at regional and national levels. With an indication of how long an epidemic may last by the end of week two, it becomes possible to estimate whether vaccination would be economically viable from the start of an epidemic. Where FFI indicates that an epidemic is unlikely to last for as long as an export ban on agricultural produce, it may be inappropriate to implement a policy of 'vaccination to live'. Alternatively where FFI indicates that an epidemic will equal or exceed the ban length, then the benefits of vaccination should be considered at an early stage, during or after the first fortnight. Since blanket vaccination of the national or regional herds and flocks would be both costly and heighten the risk of producing carrier animals, targetting vaccination through risk assessment becomes useful.  相似文献   

Control of the taeniosis/cysticercosis complex: future developments     

Flisser A  Rodríguez-Canul R  Willingham AL 《Veterinary parasitology》2006,139(4):283-292

Cysticercosis is due to the establishment of the larval stage of the zoonotic cestode parasite Taenia solium. The infection causes substantial human morbidity and mortality, particularly in several Latin American countries and parts of Africa and Asia, as well as economic losses in pig husban dry due to condemnation of infected pork meat. The life cycle of T. solium includes human beings as definitive hosts and pigs as intermediate hosts. Cysticercosis is acquired by the ingestion of eggs released by human tapeworm carriers, who become infected after ingesting pork meat contaminated with cysticerci. Taenia solium transmission has been associated with poverty, lack of sanitary services and practices of rearing backyard pigs with free access to the areas that villagers use as toilets, as well as cultural behaviour. Nonetheless, due to the recent increase of migration and tourism, industrial countries are also reporting cases of human cysticercosis. There are many epidemiological studies that have been conducted mainly in Latin American countries that have evaluated intervention measures for control of cysticercosis including the development and testing of vaccines. Furthermore, the involvement of international agencies and institutions, such as the World Health Organization, the Food and Agriculture Organization and the International Livestock Research Institute, as well as the commitment of policymakers, scientists and field workers, are key means for the sustainable control and, hopefully, eradication of T. solium infections.  相似文献   

Vaccination Strategies for Mastitis     

R.J. Erskine 《Veterinary Clinics of North America: Food Animal Practice》2012

  相似文献   

Monitoring and identification of residues of anabolic preparations in slaughtered cattle by HPLC with diode array detection     

E. H. J. M. Jansen  H. van Blitterswijk  R. W. Stephany 《The Veterinary quarterly》2013,33(2):60-65

Summary

The new combination of isocratic high performance liquid chromatography (HPLC) with on line UV spectrum detection via a diode array configuration has been applied to the detection and identification of anabolics present in application sites of cattle.

Combination of the characteristic retention time in the HPLC chromatogram and a comparison of the full spectrum between 190–400 nm of the anabolic components with that of a standard resulted in a very reliable identification. By means of this method 117 samples of application sites were investigated for the presence of anabolic residues. Of the xenobiotic anabolics, 19‐nortestosterone (NT) was found most frequently (in 96 cases), whereas diethylstilbestrol (DES) was found in only 11 cases.

In all samples the identification of NT and DES was confirmed by high resolution gas chromatography‐mass spectrometry (GCMS).  相似文献