Variation of the storage protein phaseolin in common bean (Phaseolus vulgaris L.) from the Mediterranean area     

Lucia Lioi 《Euphytica》1989,44(1-2):151-155

Summary Variation of phaseolin, the major storage protein in Phaseolus vulgaris L., was analysed in seeds of a germplasm collection from the Mediterranean area. Results showed a number of subvariants differing from the reference patterns for the presence, absence or faintness of single polypeptides. Maximum concentration of variation was found in the Cyprus material. Sources and causes of variation are discussed.  相似文献   

Comparison of some selection procedures and objectives in faba beans (Vicia faba L.). I. direct and index selection for seed yield     

D. L. C. Brinkhorst Van Der Swan  A. Ph. De Vries 《Euphytica》1986,35(3):793-808

Summary Starting with two heterogeneous F₂ populations, each based on 20 cross combinations, different selection procedures were applied for four consecutive selection cycles. Three of the selection procedures involved seed yield per plant (SYP): (i) direct selection for SYP (DSY); (ii) indexselection for SYP (ISY) with plant length, the ratio of the vegetative top to plant length, end of grain filling and duration of the generative stage as auxiliary characters and finally; (iii) independent culling levels selection (ICL) for the index described above and crude protein content. For ICL-selection the attention is focussed on SYP in this paper.For all three selection procedures, predicted and realized responses were calculated and expressed as percentage of a corresponding non-selected population. The intensities of selection differed over selection methods and generations, mostly due to practical constraints.As far as predicted responses are concerned, ISY-selection seemed to be slightly superior to DSY-selection and DSY-selection to ICL-selection. This order may partly be due to differences in selection intensity. However, none of the predictions was very accurate, but the predictions of ICL-selection seemed to be the most accurate. This has been ascribed also to the relative mild selection intensity applied in ICL-selection. It is concluded that a decision in favour of a complicated selection procedure like index selection based on predicted responses only cannot be defended. As far as realized responses are concerned, DSY-selection seems to be slightly superior to the other two selection procedures, as it is the only procedure that resulted in a more or less stable positive response to selection.The auxiliary characters, especially plant length, showed a marked correlated response to all three selection procedures. In case of direct selection for SYP this seemed paradoxical with the inefficiency of index selection, but, it may reflect the differences in the genuine genotypic correlations and the estimated genotypic correlations.  相似文献   

Effect of selection for pollen grain size on various traits in common bean     

Consuelo Montes-R  Jeffrey W. White 《Euphytica》1996,90(1):59-63

Summary Selection among microgametophytes usually exploits variation in pollen grain germination. Studies of variation in pollen grain size in common bean (Phaseolus vulgaris L.) suggested that selection for size might lead to changes in sporophytic traits. To determine whether microgametophytic selection based on size would affect pollen grain size in subsequent generations or sporophytic traits that were correlated with pollen grain size, pollen grains from three crosses were separated into two size categories by sieving and then used to pollinate cv. Diacol Calima. Selection resulted in changes in pollen grain diameter for pollen from F₁, F₂ and F₃ plants for all crosses. In vitro germination indicated no differences between vigor of large and small grains, but extraction and sieving reduced germinability. F₁ seed from two of the crosses with size-selected pollen varied in weight according to pollen grain size, but in subsequent generations, the effect disappeared. Both size categories of selected pollen resulted in F₂ progeny with reduced numbers of seeds per pod as compared to controls, suggesting that the size selection process may have resulted in indirect selection for traits reducing seed set. The overall results suggested that genes determining pollen grain size in bean have little or no effect on sporophytic traits such as seed size and seed yield.  相似文献   

Breeding the common bean (Phaseolus vulgaris L.) for resistance to bean common mosaic virus: alternatives to backcrossing     

O. Z. Mukoko  N. W. Galwey 《Euphytica》1995,82(2):91-104

A series of field experiments was undertaken in order to determine whether resistance to bean common mosaic virus (BCMV) could be incorporated into genotypes of the common bean (Phaseolus vulgaris L.) suitable for cultivation in Zimbabwe without recourse to backcrossing. Six inbred genotypes carrying the resistance-conferring alleles at the loci I and Bc-3 were crossed with five locally-adapted inbred genotypes. The first experiment comprised F₃ progeny rows, each derived from a single unselected F₂ plant, the second, F₃ bulks selected for resistance, and the third, a comparison of selected and unselected F₂-derived F₄ lines. The number of days to flowering and to maturity, the incidence of mosaic and necrosis symptoms, seed yield and seed size were recorded. There was evidence that late flowering and maturity were associated with BCMV resistance in some crosses, though not strongly enough to present an obstacle to plant breeding. The incidence of virus symptoms and seed yield were influenced by genetic factors additional to the major resistance genes, and variation in seed yield was present not only between bulk populations of crosses, but also between single-row plots of lines within crosses. This indicates that early-generation selection for yield in the presence of BCMV, even among progeny selected for BCMV-resistace, is likely to be effective. However, the variation in yield among F₄ lines was least in the highest-yielding crosses, which may represent a limit to successful selection for yield. Seed size was partly under additive genetic control, but there was also evidence of non-allelic interactions. There was no association between large seed size, preferred by consumers, and susceptibility to BCMV in the progeny, indicating that the association between these characters in the parent lines is fortuitous and will not present an obstacle to plant breeding. It is noted that a considerable amount of useful genetic information can be obtained without recourse to elaborate crossing schemes, provided that unselected progeny are included in experiments as controls. The evidence presented indicates that resistance to BCMV can be combined with appropriate values of maturity date, yield and seed size without the need for backcrossing.  相似文献   

New Isozyme Markers in Vicia faba: Inheritance and Linkage     

M. J. Suso    M. T. Moreno  J. I. Cubero 《Plant Breeding》1993,111(2):170-172

Results on the inheritance of 6 enzyme systems: LDH, PGM, FDH, SKD, SOD, AAT from seeds of Vicia faba and the linkage relationships among these isozyme loci are presented. The allozymes at each one of these loci behaved in a codominant manner and segregated in the expected Mendelian ratio. Linkage tests between these loci showed that they segregate independently.  相似文献   

Genetic interpretations of the effects of bulk breeding on four populations of beans (Phaseolus vulgaris L.)     

John Hamblin  J. R. Morton 《Euphytica》1977,26(1):75-83

Summary The effects of bulk breeding on yield and its components in two high-yeilding and two low-yielding bean populations when grown at crop densities are interpreted in terms of gene action and selection. There was considerable additive variation in the crosses, dominance effects were variable but tended to be negative. The results were compared with results obtained at low density by another worker with the same populations, and this showed that interpretations of gene action in hybrid vigour depend on the test environment used. The interpretations were also compared with plant breeding interpretations made on the same material (Hamblin, 1977), and it was found that a single pattern of response to natural selection, in plant breeding terms, may be explained by more than one genetic interpretation and, conversely, a single genetic interpretation may result in different patterns of response in plant breeding terms. The value of composite crosses in plant breeding are discussed and it is concluded that there is a greater chance of producing improved lines from a large number of simple crosses than from a smaller number of composite crosses.  相似文献   

Yield, yield components and plant architecture in the F3 generation of common bean (Phaseolus vulgaris L.) derived from a cross between the determinate cultivar ‘Prelude’ and an indeterminate landrace     

Mohamed I. Dawo  Francis E. Sanders  David J. Pilbeam 《Euphytica》2007,156(1-2):77-87

Growth trials were conducted outdoors in the UK to determine the yield, yield components and plant architectural differences between determinate and indeterminate Phaseolus vulgaris bean plants. F3 lines derived from crosses between ‘Prelude’, a determinate cultivar and ‘V8’, an indeterminate landrace, were grown together with the parents and ‘Carioca’, an indeterminate landrace from Brazil. Data were recorded on flowering date, number of nodes on main stem at flowering, plant height at maturity, number of pods/plant, number of seeds/pod, number of seeds/plant, 100-seed weight, seed mass/plant, percentages of diseased and healthy seeds/plant. Determinate F3 lines had significantly lower (P < 0.05) seed mass/plant, fewer pods/plant, fewer seeds/pod, fewer seeds/plant, lower harvest index, shorter stems, earlier date of flowering, fewer nodes at flowering and fewer healthy seeds/plant than indeterminate F3 lines. However, determinate genotypes had significantly larger (P < 0.05) pods to accommodate their larger seeds compared with indeterminate lines. A similar situation was found when the yield and yield components of ‘V8’, ‘Prelude’ and ‘Carioca’ were evaluated; the highest seed mass/plant was given by ‘V8’ and the lowest by ‘Prelude’. Furthermore, some indeterminate genotypes with Type IV growth habit yielded significantly higher (P < 0.05) than the high-yielding parent, ‘V8’, indicating a positive influence on seed yield by crossing different genotypes.  相似文献   

Screening techniques and sources of resistance to abiotic stresses in cool-season food legumes     

F. L. Stoddard  C. Balko  W. Erskine  H. R. Khan  W. Link  A. Sarker 《Euphytica》2006,147(1-2):167-186

Summary The adaptability and productivity of cool-season food legumes (chickpea, faba bean, lentil, pea) are limited by major abiotic stresses including drought, heat, frost, chilling, waterlogging, salinity and mineral toxicities. The severity of these stresses is unpredictable in field experiments, so field trials are increasingly supplemented with controlled-environment testing and physiological screening. For drought testing, irrigation is used in dry fields and rain-out shelters in damp ones. Carbon isotope discrimination (Δ¹³C) is a well-established screen for drought tolerance in C3 cereal crops which is now being validated for use in grain legumes, but it is relatively expensive per sample and more economical methods include stomatal conductance and canopy temperature. Chickpea lines ICC4958 and FLIP87-59C and faba bean line ILB938 have demonstrated good drought tolerance parameters in different experiments. For frost tolerance, an efficient controlled-environment procedure involves exposing hardened pot-grown plants to sub-zero temperatures. Faba beans Cote d’Or and BPL4628 as well as lentil ILL5865 have demonstrated good freezing tolerance in such tests. Chilling-tolerance tests are more commonly conducted in the field and lentil line ILL1878 as well as derivatives of interspecific crosses between chickpea and its wild relatives have repeatedly shown good results. The timing of chilling is particularly important as temperatures which are not lethal to the plant can greatly disrupt fertilization of flowers. Salinity response can be determined using hydroponic methods with a sand or gravel substrate and rapid, efficient scoring is based on leaf symptoms. Many lines of chickpea, faba bean and lentil have shown good salinity tolerance in a single article but none has become a benchmark. Waterlogging tolerance can be evaluated using paired hydroponic systems, one oxygenated and the other de-oxygenated. The development of lysigenous cavities or aerenchyma in roots, common in warm-season legumes, is reported in pea and lentil but is not well established in chickpea or faba bean. Many stresses are associated with oxidative damage leading to changes in chlorophyll fluorescence, membrane stability and peroxidase levels. An additional factor relevant to the legumes is the response of the symbiotic nitrogen-fixing bacteria to the stress.  相似文献   

Genetics of colour traits in common vetch (Vicia sativa L.)     

Doza M.S. Chowdhury  Jane M. Rathjen  Max E. Tate  Glenn McDonald 《Euphytica》2004,136(3):249-255

Five parents of common vetch (Vicia sativa L.) having orange/beige cotyledon colour, brown/white testa colour, purple/green seedling colour and purple/white flower colour were crossed as a full diallele set. The inheritance patterns of cotyledon, testa or seed coat colour, flower and seedling colour, were studied by analyzing their F₁, F₂, BC₁ and BC₂ generations. The segregation pattern in F₂, BC₁ and BC₂, showed that cotyledon colour was governed by a single gene with incomplete dominance and it is proposed that cotyledon colour is controlled by two allelic genes, which have been designated Ct₁ and Ct₂. Testa colour was governed by a single gene with the brown allele dominant and the recessive allele white. This gene has been given the symbol H. Two complementary genes governed both flower and seedling colours. These flower and seedling colour genes are pleiotropic and the two genes have been given the symbols S and F.  相似文献   

Present status and future strategy in breeding faba beans (Vicia Faba L.) for resistance to biotic and abiotic stresses     

D. A. Bond  G. J. Jellis  G. G. Rowland  J. Le Guen  L. D. Robertson  S. A. Khalil  L. Li-Juan 《Euphytica》1993,73(1-2):151-166

Progress is being made, mainly by ICARDA but also elsewhere, in breeding for resistance to Botrytis, AScochyta, Uromyces, and Orobanche; and some lines have resistance to more than one pathogen. The strategy is to extend multiple resistance but also to seek new and durable forms of resistance. Internationally coordinated programs are needed to maintain the momentum of this work.Tolerance of abiotic stresses leads to types suited to dry or cold environments rather than broad adaptability, but in this cross-pollinated species, the more hybrid vigor expressed by a cultivar, the more it is likely to tolerate various stresses.  相似文献